ii range of eligible guarantors counter guarantors prote
Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt
Ngày tải lên :
07/03/2014, 16:20
... Arg78 and the effect of the reduction of cationicity of MBP in the triggering of multiple sclerosis Flexible docking calculations of the MBP(74–85) epitope with the MHC II I-Au recognition site ... from the side chain of Arg78 The side chain of Arg78 is less well defined, as in the case of aqueous solution, because of the absence of interactions with the side chains of Asp81 (in the agonist ... (Table 1) The evolution of the radius of gyration, which reflects the compactness of 3404 A G Tzakos et al (Eur J Biochem 271) Ó FEBS 2004 Fig Evolution of the radius of gyration of the MBP(74–85) and...
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Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
Ngày tải lên :
30/03/2014, 02:20
... association to DNA of Jun proteins in the induction of the AGT gene by Ang II in VSMCs ChIP assays confirmed that Ang II increased the recruitment of AP-1 to the AGT gene promoter Overexpression of c-Jun ... activation of STAT5B and c-Jun, and STAT5B and AP-1 interaction in AGT gene activation the expression of the AGT gene in VSMCs stimulated with Ang II, the effect of Ang II on the phosphorylation of STAT5 ... AGCTTCTGCTTACCTTTAGCTCCAGCC-3¢ (HindIII site underlined), digested by KpnI and HindIII, and then inserted into the pGL3-basic luciferase reporter gene vector (Promega, Madison, WI, USA) linearized by KpnI–HindIII and named...
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báo cáo hóa học:" Use of a novel cell-based fusion reporter assay to explore the host range of human respiratory syncytial virus F protein" ppt
Ngày tải lên :
20/06/2014, 04:20
... The mature form of the F protein present on the surface of the virus and infected cells is believed to consist of a homotrimer consisting of three non-covalently associated units of F1 disulfide ... L138R A) Comparison of fusion activity of wild-type and a fusion peptide mutant of HRSV F Figure A) Comparison of fusion activity of wild-type and a fusion peptide mutant of HRSV F 293T cells ... of quantifying the fusion activity of the HRSV F protein This assay has multiple applications For example, this assay can be used as a means of studying the structure-function of the HRSV F protein,...
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Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
Ngày tải lên :
18/02/2014, 11:20
... confirmed the identity of the purified protein as HSL (data not shown) The yield of pure protein was mg per litre of insect cell culture The specific activities of the purified protein against TO, ... performance of the assay was reliable only within a limited range of probe concentrations), dialysis after phosphorylation was not necessary, probably because of a lower degree of interaction of ATP ... with both bis-ANS and SYPRO Orange, creating high levels of background fluorescence, we investigated the possibility of using lower amounts of ATP for the phosphorylation of HSL Using radiolabelled...
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Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf
Ngày tải lên :
18/02/2014, 16:20
... behavior of Fe (II) , because of the similarity of ionic radii and paramagnetic properties [31] It should produce efficient line broadening of ˚ atoms closer than A Accordingly, complete bleaching of ... No of protein atoms No of water molecules Number of metal ions ˚ Bond lengths (A) Bond angles (°) Protein Metal ions Occupancy of sites M1, M2, M3, M4 and M5 Ramachandran plot (%) CyaY–Co (II) ... rearrangements (B) Details of the superimposition of the hydrophobic core of CyaY (blue) and CyaY–Eu complex (red) The side chains of the CyaY C-terminus rearrange as a longrange effect of binding The figure...
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Tài liệu Báo cáo khoa học: N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity ppt
Ngày tải lên :
19/02/2014, 00:20
... of the counterpart S castellii residues of inhibitor 12 Both inhibitors had comparable Ki values against PpPr (15 and 12; Fig 4) Consequently, the nature of residues 35–68 at the C-terminus of ... benefit of these alterations at each extremity of the polypeptide chain, must of necessity arise from contacts that are made outwith the active site of the enzyme The effect of removal of the ... occupation of the active site of the target proteinase by the inhibitory segment For ease of interpretation, residues in the inhibitors are denoted by single letter abbreviations while those from the proteinase...
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Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx
Ngày tải lên :
19/02/2014, 05:20
... shift the M-Fe(III)-H ! H-Fe(III)-H equilibrium towards the bis-H species, and destabilize the M–Fe(III)–H coordinated form The reduced effect of Cl– reflects the different affinities of the two anions ... M–Fe(III)–H coordination, and a bishistidine coordinated species The equilibrium between these two low-spin forms, here indicated as M-Fe (III)-H ! H-Fe(III)-H is strongly influenced by the type of ... structured forms of proteins and are often employed in studies aimed at clarifying aspects of the folding process of proteins [34], including cytochrome c [18] Concerning this last protein, fast...
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Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc
Ngày tải lên :
19/02/2014, 08:20
... levels of a larger number of lysozyme variants, a highly significant correlation has been identified between the level of secreted protein and the thermostability of the native state of the protein ... suggesting that the overall structure of the folded proteins is unlikely to differ significantly from that of the WT protein The formation of a significant amount of one or more partially unfolded ... less stable proteins is a result of degradation of partially folded or misfolded proteins by the quality control system during secretion In light of the correlation between secreted protein levels...
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Tài liệu Managed Investment Funds Product Disclosure Statement - A range of funds that allows you to create an investment portfolio that suits your individual needs ppt
Ngày tải lên :
19/02/2014, 09:20
... to ensure that we select managers of the highest quality Our funds We offer a range of funds to help meet your investment needs You can choose from a wide range of funds that invest in different ... effectiveness of provisions of this kind You can inspect a copy of the Constitution at our head office, or we will provide you with a copy free of charge The Constitution gives us a number of rights, ... Power of Attorney, please comply with the following: WW attach a certified copy of the Power of Attorney document WW each page of the Power of Attorney document must be certified by a Justice of...
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Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
Ngày tải lên :
19/02/2014, 12:20
... EI (enzyme I of the PTS), the rst protein in the cascade of proteins forming the PTS, to HPr, the histidine-phosphocarrier protein HPr is the smallest protein in the protein cascade of the PTS ... presence of urea Biochemistry 34, 67956804 53 Privalov, P.L & Makhatadze, G.I (1990) Heat capacity of proteins II Partial molar heat capacity of the unfolded polypeptide chain of proteins: protein ... stability of a protein In Protein Structure (Creighton, T.E., ed.), 2nd edn, pp 253259 Oxford University Press, Oxford 35 Privalov, P.L (1979) Stability of proteins: small globular proteins Adv Protein...
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Tài liệu Báo cáo khoa học: Altered inactivation pathway of factor Va by activated protein C in the presence of heparin doc
Ngày tải lên :
19/02/2014, 13:20
... presence of 200 nM protein S The resulting time course of inactivation of FVa by APC became monophasic, an effect known to be due to stimulation of the cleavage at Arg306 [14] Addition of 10 IUÆmL)1 ... amount of prothrombin activated was then determined using S-2238 [2] APC-catalyzed inactivation of FVa Time courses of FVa inactivation by APC were determined by following the loss of FXa cofactor ... orientation of a protease associated with a substrate/inhibitor as seen in the X-ray crystal structure of a Michaelis serpin–protease complex (file 1l99 [40]) to optimize further the positioning of the...
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Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx
Ngày tải lên :
19/02/2014, 16:20
... conservation of charged residues is comparable with the overall homology of these structures, being in the range of 30–40% Table Comparison of structural features of 1SH7, 1IC6 and 1THM 1SH7 Number of ... modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases Protein Eng 4, 719–737 Schechter I & Berger A (1967) On the size of the active site in proteases ... stability of the Vibrio proteinase are being tested Elucidation of the Vibrio protein833 ´ ´ J Arnorsdottir et al Structural aspects of cold adaptation ase structure, the first structure of a cold-adapted...
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Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspecific alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf
Ngày tải lên :
19/02/2014, 17:20
... Degradation and ubiquitination of TNSALP (1559delT) We next examined the effect of several protease inhibitors on the degradation of TNSALP (1559delT) Inhibitors of proteasome function, such as ... the degradation of TNSALP (1559delT) (Fig 5A,B), indicative of involvement of the proteasome Consistent with this observation, leupeptin and pepstatin A (inhibitors of lysosomal proteases) had ... oligosaccharide chains of glycoproteins [31] The involvement of EDEM and ⁄ or SCFFbs2 in the degradation of TNSALP mutant proteins is currently being investigated The aggregate form of TNSALP (1559delT)...
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Tài liệu Báo cáo khoa học: Consequences of COP9 signalosome and 26S proteasome interaction doc
Ngày tải lên :
20/02/2014, 01:20
... formation of super-complexes consisting of the CSN, the 26S proteasome and Ub ligases The rationale for generating a chimerical protein consisting of the N-terminal part of CSN2 and the PCI domain of ... a direct interaction of the CSN with the proteolytic machinery of the Ub system, the 26S proteasome Several years ago it was shown that the CSN cofractionates with the 26S proteasome from human ... indicate an association of the CSN and the 26S proteasome, the exact mode of this interaction, the role of PCI domains, and its consequences for the stability of cellular proteins is not known...
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Tài liệu Range of practices and issues in economic capital modelling pdf
Ngày tải lên :
20/02/2014, 19:20
... list is often a helpful indication of the level of understanding of the model (vi) Examination of assumptions - sensitivity testing Models rest on assumptions of various kinds, some of which ... economic capital models In Section II the paper covers the use of economic capital models and the governance and control framework Section III reviews the range of risk measures used by banks in ... determination of the adequacy of the absolute level of capital Critical issues include: (i) comprehensive capture of the risks by the model; (ii) diversification assumptions; and (iii) assumptions about...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
Ngày tải lên :
20/02/2014, 23:20
... intensity of the band of unhydrolyzed actin was plotted against the time of incubation Characterization of actin preparation quality Fluorescence parameters were used for estimation of nativity of actin ... Actin and small heat shock protein (Eur J Biochem 270) 895 Fig Fluorescence spectra of 140 lM ANS in the absence of added proteins (1) and in the presence of 2.25 lM of intact actin (2) or actin ... polymerization of intact actin and aggregation of heated actin Interaction of HSP25 with intact actin Fig Effect of heating on the kinetics of polymerization (A) and saltinduced increase of the light...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf
Ngày tải lên :
21/02/2014, 03:20
... variants were calculated with the INSIGHT II/ DISCOVER software The radius of the solvent ˚ probe was 1.4 A Measurement of 1H-NMR The pH-dependent 1H-NMR of wild-type API was measured in order to ... effect of the size of the residue at position 169 may be related to the solvent accessibility of the side-chain of His210 (Fig 3C) When the accessibility of the side-chain of His210 increased, ... characteristics of Achromobacter lyticus protease I, a lysine-specific serine protease J Biol Chem 264, 3832–3839 Perona, J.J & Craik, C.S (1995) Structural basis of substrate specificity in the serine proteases...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
Ngày tải lên :
21/02/2014, 15:20
... presence of a 20-fold mass excess of the substrate protein Contrarily to what expected for a generic protective effect of added proteins, the residual activity after heat treatment in the presence of ... the appropriate proteolytic enzyme were added at °C to mL of BLG (2.5 mgÆmL)1 in 50 mM phosphate buffer, pH 6.8) to a final mass ratio enzyme/BLG of : 20 or of : 10 The protein/protease mixture ... denaturation and aggregation of BLG upon heat treatment may hide putative sites of attack from the action of proteases, therefore leaving intact some regions of the protein that may be relevant...
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Tài liệu Báo cáo Y học: Re-oxygenation of hypoxic simian virus 40 (SV40)-infected CV1 cells causes distinct changes of SV40 minichromosome-associated replication proteins doc
Ngày tải lên :
22/02/2014, 04:20
... Differential pattern of minichromosome-bound replication proteins occur before and after re-oxygenation of hypoxic cells Fig Alkaline sucrose gradient centrifugation of viral DNA of minichromosomes ... synchronous round of viral replication The present study intended to further characterize the hypoxia-induced inhibition of unwinding by analysis of the binding of distinct replication proteins to ... nucleotides of single-stranded DNA Formation of the RPA30nt-complex may be followed by phosphorylation of the RPA-34 subunit [30,31,49] Partial denaturation of the central palindrome leads to loss of...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... Scientific Services Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum ... relative amount of UP12 (d) were measured for 12 samples of cells taken at the indicated time (B) Western blot analysis of proteins in samples collected in (A) lg and lg of total protein in samples ... synthesized unfolded proteins with the heat-shock protein GroEL Nature 336, 254–257 25 Lorimer, G.H (1996) A quantitative assessment of the role of the chaperonin proteins in protein folding in...
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