ihc detection of protein expression and localization

... (2 M) and used for estimation of the protein content using the Lowry procedure [19] and BSA as protein standard The residual 1000 lL of the suspension was centrifuged (20 000 g, 10 min) and the ... Figs and the active taurine uptake correlates with TauT expression In order to confirm changes in subcellular level of TauT expression and TauT localization upon supplementation of taurine and ... stimulation of PKC and PKA Stimulation of PKC and PKA has no detectable effect on the expression of TauT in NIH3T3 fibroblasts (data not shown) and most probably does not involve modulation of the...

Ngày tải lên: 30/03/2014, 15:20

... and quadrupole time -of- flight mass spectrometry Grouping of identified proteins A, Proteins that are known to be nuclear matrix proteins; B, proteins that are known to be nuclear proteins; C, proteins ... with a protein sequencer and peptide mass finger printing, respectively ‘No of peptides’ indicates the number of peptides of which partial amino acid sequences were determined Proteins 2, 20 and ... MS, and consisted of novel proteins along with many known structural proteins One of these novel proteins was shown to be located to compartments in the interchromatin space in the nucleus The protein...

Ngày tải lên: 30/03/2014, 20:20

... Immunofluorescent localization of inhibin α, βA, and βB isotypes in mouse testis at postnatal days 48 (A and B) Double-immunofluorescent staining in the same section showed the co -localization of ... Yujin Kim et al Temporal expression pattern of the inhibin isotypes α, βA and βB during the postnatal development of mouse testis The protein levels of the inhibin α, βA and βB isotypes in the ... (approximately 1.5 fold, p ＜ 0.01 vs PND 1-18) and PND 120 (approximately 1.6 fold, p ＜ 0.05 vs PND 1-18) (Figs 2A and D) Immunofluorescent detection of inhibin α, βA and βB in mice testis At PND 1-6, there...

Ngày tải lên: 07/08/2014, 23:22

... in situ effect of licofelone on the gene expression and protein synthesis of the major collagenolytic enzymes (MMP-13 and cathepsin K) and aggrecandegrading proteases (ADAMTS-4 and ADAMTS-5) in ... exists between the mRNA and protein levels At the two dosages tested, the R1094 ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis The level of gene expression of ADAMTS-5 in OA cartilage ... The results of a number of studies indicate that the regulatory mechanisms of ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis are complex and may vary based on species and culture...

Ngày tải lên: 09/08/2014, 06:23

... staining of the cells and mean values of at least 100 cells randomly selected on each sample were 24% and 46% for infected and control cells respectively HSP70 protein increases during infection and ... cell protein and nucleic acids synthesis inhibition, depending on time and an infectious dose Despite the observed shutdown of host transcriptional and translational mechanisms and selective expression ... important role of the nuclear pool of HSP70 [5] Our data showing the predominant nuclear localization of HSP70 not support the hypothesis on the possible role of HSP70 in folding of viral proteins...

Ngày tải lên: 11/08/2014, 08:21

... instance, cell lines latently infected with HIV-1 and KSHV were used to generate profiles of viral gene expression in the presence of cyclin-dependent Page of (page number not for citation purposes) ... post-translational modifications of histone proteins Methylation, acetylation, and/ or phosphorylation of certain amino acid residues at the amino terminal "tails" of histone H3 and/ or H4 can indeed influence ... Kashanchi F: Use of a multi-virus array for the study of human pathogens: gene expression studies and ChIP-chip analysis Retrovirology 2004, 1:10 Kuo MH, Allis CD: In vivo cross-linking and immunoprecipitation...

Ngày tải lên: 13/08/2014, 13:20

... formation of dimeric nanostructure with DLS for the detection of the ER protein The presence of the ER-binding site on the dimers allowed the binding of the ER, and which formed the basis for the detection ... synthesis of AuNPs of 15nm size and smaller In the work by Handley, the combination of tannic acid as a co-reductant with citrate resulted in AuNPs of 10nm size and below with good monodispersity and ... application of MBs in the detection of protein biomarkers was realized in the development of 25 aptamer beacons in which the recognition of aptamers to specific proteins resulted in the restoration of...

Ngày tải lên: 09/09/2015, 11:16

... number of caveolae and strong expression of Caveolin-1 [24–26] After demonstrating robust levels of P2X7 protein expression in the lung epithelial cell line E10, we studied the localization of P2X7 ... presence of the 80 kDa band alone Isolation of lipid rafts and localization of Caveolin-1 and P2X7 Insolubility in the detergent Triton X-100 is a wellrecognized biochemical characteristic of proteins ... downregulation of Caveolin-1 expression leads to significantly reduced levels of P2X7 protein expression in E10 cells These data imply that Caveolin-1 regulates the expression level of the P2X7 receptor...

Ngày tải lên: 19/02/2014, 00:20

... percentage of contaminated cultures; review question B: the name and manufacturer of the reference standard • Details of index test: software version of test • Details of comparator: type of microscopy: ... 2010a) Early and accurate diagnosis and effective treatment is the cornerstone of TB care and control (Dye 2010) A basic tenet of early and accurate diagnosis is the identification of smear-negative ... addresses question A (detection of MTB) and/ or B (detec- - A tion of RIF resistance)? 2-B - A and B What was the purpose of this study? * if smear results are stratified, - Only accuracy of Xpert as replacement...

Ngày tải lên: 06/03/2014, 04:20

... dithiothreitol, mM ATP, and 0.05% BSA in the presence of the indicated concentration of endoG for 10 at 37 °C The reaction was stopped by the addition of lgÆmL)1 proteinase K and 0.5% SDS, and then incubated ... NaCl and 1.0% Triton X-100 were added, and the samples were centrifuged at 100 000 g for 30 and separated into pellets and supernatants The pellets were suspended in the same volume of buffer, and ... 3, 5, and 7) and 1.0 lg (lanes 2, 4, 6, and 8) of template DNA, respectively Signals marked with * may be nonspecific artifacts caused by stalling of the extension reaction (B) Light strand The...

Ngày tải lên: 23/03/2014, 21:20

... [5,7] Carels and Bernardi analyzed the contigs of A thaliana and concluded that the GC level of genes and coding regions, as well as gene densities and expression level showed to be evidently higher ... homogeneity of isochore can be defined by an index h [17,20], which is defined as the variance of GC content of the region divided by that of the whole genome If h ( 1, the variance of GC content of the ... orange dots in Fig 1, and they are located in 0–230 kb of chromosomes II and 0–350 kb of chromosomes IV, respectively The similar genomic organization of chromosomes I, III and V makes their overall...

Ngày tải lên: 30/03/2014, 20:20

... precision of 74% and recall u of 60%, and K-nearest neighbors in TiMBL: both in (Evans, 2001) with precision of 73% and recall of 69%, and in (Boyd et al., 2005) with precision of 82% and recall of ... definitions and values tense, and mood with the preceding verb in the sentence and also with the main verb of the sentence.3 7-9 NHPREV, NHTOT, INF: the candidates for the subject of the clause ... Alexander Clark, Chris Fox, and Shalom Lappin, editors, The handbook of computational linguistics and natural language processing, pages 599–629 Wiley Blackwell, Oxford C M¨ ller 2006 Automatic detection...

Ngày tải lên: 31/03/2014, 21:20

... evaluate and compare the modulations of PBMC global gene expression profiles induced by IFNa in melanoma and normal donors The effects of the administration of different doses of IFNa, as well as of ... level of refractoriness and can occur after inoculation of as little as millions of units of IFNa iii) The observed molecular signature is paralleled by the raise in percentage and expression of ... appearance of the IFNa signature, and additional rounds of increase were observed at 24 hr after the subsequent IFNa treatments, in parallel with the de novo detection of an up-regulated expression of...

Ngày tải lên: 18/06/2014, 19:20

... effects of glucose and/ or phosphate were examined in Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System 75 Ta ble Compostion of ... mM in SOF [6] and TLP [11], and 5.56 mM in TCM199 The beneficial effects of co-culture included a reduced glucose concentration, an increase in the levels of L-lactate and pyruvate [5], and a ... V) e Fetal bovine serum Ta ble Effect of protein source on the in vitro development of 2-cell bovine embryos Protein source No of embryos cultured* No (%) of blastocysts Mean blastocyst cell no...

Ngày tải lên: 07/08/2014, 17:22

... levels, facilitated the discovery of novel splice variants, and enabled detection of novel fusion transcripts and isoforms thereof that would otherwise have escaped detection As such, this method ... to of 887 target transcripts 220,151 6,364,131 On-target specificity 5% Figure Preservation of expression levels of target transcripts in hybrid selection Preservation of expression levels of ... understanding of the molecular state of the transcriptome Finally, this method is easily scalable to larger numbers of samples and genes Our targeted RNA-Seq method provides a direct and powerful...

Ngày tải lên: 09/08/2014, 20:20

... presence of a heterogenous mixture of variably sized clusters Furthermore, following evaporation of methanol, the majority of fullerene clusters not reaggregate, and have a range of sizes of tens of ... Photoluminescence of crystalline C60 occurs due to coupling of the vibrational modes of the lattice with electronic transitions and the PL signature of fullerene crystals may be useful to track the presence of ... indicative of C60 preparations in methanol and toluene (Figure 2) The observed mass is consistent with the formation of a positively charged C60 ion by loss of an electron instead of gain of a proton...

Ngày tải lên: 11/08/2014, 00:22

... Page of Figure The specificity and sensitivity of one step SYBR green real-time RT-PCR Plot of the amplification of a 10-fold serial dilution of ch-1a RNA to calculate the detection limit and ... serially to 10-7 and were assayed in a 25 μl reaction mixture containing μl of diluted RNA; 0.4 μM of Nf primer and 0.4 μM of Nr primer; 12.5 μl of 2× One Step SYBR RT-PCR Buffer; μl of PrimeScript™ ... RT-PCR for detection of porcine reproductive and respiratory syndrome virus Virology Journal 2010, 7:90 Figure The sensitivity of conventional RT-PCR for detection of the PRRSV The detection limit...

Ngày tải lên: 12/08/2014, 04:20

... terminus of N3T and N7T proteins Expression of NP, N3, and N7 proteins in yeast The pESC-derived expression plasmids carrying the NP, N3 and N7 protein- encoding sequences were transformed Page of 10 ... Dybkaer K, Munch M, Handberg KJ, Jorgensen PH: Application and evaluation of RT-PCR-ELISA for the nucleoprotein and RTPCR for detection of low-pathogenic H5 and H7 subtypes of avian influenza virus ... ratios at 1:100 and the prediction equation was determined Results Expression of the recombinant AIV protein The expression of the NP, N3 and N7 genes was induced by 2% galactose and the yeast...

Ngày tải lên: 12/08/2014, 04:20

... plasmid, and less than 0.5 PFU of H5N1 viruses per reaction, and specific for the detection of influenza A of subtype H5 The HA gene of clades 1, 2.1, and 2.3 was amplified from both virus isolates and ... infection and H5N1 subtyping Results Analytical sensitivity and specificity The analytical sensitivity of our LNA Taqman rRT-PCR for the detection of the HA gene of H5N1 was < 0.5 PFU of virus and ... http://www.virologyj.com/content/7/1/46 Page of Conclusions We have developed a highly sensitive and specific rRTPCR assay for the detection of H5N1 influenza A virus of both clade and directly in clinical specimens, and evaluated...

Ngày tải lên: 12/08/2014, 04:21