identification verification and analysis of structural variation

... Jolma and co-workers obtained comparable datasets using between two and four rounds of SELEX [8] Profiling of NF-B p52p52 from SELEX1 and SELEX3 revealed there was an over-representation of sequences ... Binding affinity of dimer for 11-mer sequence (z-score) Figure Binding profiles of the different NF-B dimers Heat map illustration of binding profiles obtained from microarray analysis of dimers Within ... again, the profile of RELARELA was distinct from that of the heterodimers RELAp50 and RELAp52 (Table 2) This is consistent with what we observed using microarrays where binding profiles of the two...

Ngày tải lên: 09/08/2014, 23:20

... core and cover one side of the CCP2 Although these residues are positively charged, such amino acids are often part of recognition site and the side-chain of Arg is for a large part hydrophobic and ... difluoride)-membrane and subjected to Western blot using MoAb 44 (A) Lane and show the Western blot, whereas lane and show the corresponding silver stained gels of two different C4BP preparations (lanes and 3, and ... The role of CCP2 of the C4BP b-chain in binding of protein S (Eur J Biochem 270) 97 Fig SDS/PAGE analysis of recombinant C4BP b-chain CCP1-2 (A) Indicated recombinant proteins (wild-type and mutants),...

Ngày tải lên: 17/03/2014, 09:20

... indicates the position of the ssDNA in the absence of SSB protein and the subsequent retardation of the band with an increasing amount of SSB is also shown *Position of the double-stranded control DNA ... Effect of HpSSB on ATPase activity of HpDnaB in the presence of ssDNA The release of radiolabeled Pi from (c-32P)ATP was monitored in the absence and presence of different concentrations of HpSSB ... Wt and DC20 SSB protein in varying concentrations (0, 0.45, 0.9, 1.8, 2.7 and 3.6 lg, respectively) with 300 ng of M13mp18 single-stranded circular DNA and ⁄ or 300 ng of pUC18 double-stranded...

Ngày tải lên: 30/03/2014, 02:20

... hTERT A) Flow cytometry determination of the expression of GFP and p19gag The percentage of cells in each quadrant is indicated B) Analysis of hTERT, and of telomere protein gene expression in ... transcription of TERF1 and of TERF2 underwent an increase of 1.2 to 2.4, and of 2.7 to 4.0, respectively, the transcription of POT1 rose at a much higher level (Fig 2) Indeed, the amount of Pot1 in ... activation of CD4+ T cells reveals an inverse regulation in the transcription of telomerase and that of POT1, TERF1 and TERF2 genes Transcriptional expression of hTERT, POT1, TERF1 and TERF2 genes...

Ngày tải lên: 13/08/2014, 09:21

Ngày tải lên: 17/07/2015, 20:31

... change in Trp13 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used ... change in Trp85 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used ... the calculated average values of uorescence lifetimes ặsDổ and ặsDAổ The values for average lifetimes of Trp85 in the absence and presence of acceptor and efciency of energy transfer are presented...

Ngày tải lên: 20/02/2014, 23:20

... temperature) and resuspended in Mops medium [5.5 gÆL)1 of Mops free acid, 5.1 gÆL)1 of Mops sodium salt, 0.5 gÆL)1 of NaCl, gÆL)1 of NH4Cl, 0.06 gÆL)1 of Na2HPO4Æ2H2O, 0.05 gÆL)1 of KH2PO4, mm ... Physical and functional analysis of the prokaryotic enhancer of the r54-promoters of the TOL plasmid of Pseudomonas putida J Mol Biol 258, 562–574 22 Sze CC, Laurie AD & Shingler V (2001) In vivo and ... (devoid of parts of their A-domain) were constructed One of these, the DA-HbpR variant (in plasmid 1759 HbpR-binding site D Tropel and J R van der Meer Fig DNaseI footprinting analysis of the...

Ngày tải lên: 07/03/2014, 17:20

... conformation of the (+)-cis adduct and the minorgroove conformation of the (+)-trans adduct, the latter interfering with interactions of the catalytic loops of the MTases and the minor groove of DNA ... alone and in complexes with MTases, the fluorescence intensities at 384 nm were calculated Determination of the amounts of the active form of M.SssI and M.HhaI The amount of the active form of M.SssI ... with the levels of DNA methylation, and thus the effect of the absolute configurations and conformations of the adducts on DNA methylation was not evaluated More recently, the effect of stereochemically...

Ngày tải lên: 19/02/2014, 00:20

... the p53 protein and activate an analogous set of downstream genes Multiple splice isoforms of the p73 protein have been found that differ in the structure of their N-terminal and ⁄ or C-terminal ... DNA comprises about 50% of 1,2-GG IACs, 25% of 1,2-AG IACs, 10% of 1,3-GNG IACs and interstrand crosslinks, and another 2–3% of monofunctional adducts It has been found that cisplatin cytotoxicity ... respective supershifted bands are denoted as SR53, SR73d and SR73b; the presence of two supershifted bands in each of the lanes 5–7 corresponds to two possible stoichiometries of the antibody–protein...

Ngày tải lên: 19/02/2014, 05:20

... eukaryotic and archaeal proteins (Fig 1A) Like the case of bacterial RecA, the L1 and L2 loops of the eukaryotic Rad51 proteins face A Roles of the HsRad51 L1 and L2 loops inside of their helical ... HsRad51-L1 loop and measured the fluorescence changes of the tryptophan residues upon DNA binding Results Strategy of mutational analysis In order to study the functions of the L1 and L2 loops of HsRad51, ... Roles of the HsRad51 L1 and L2 loops D231W (L1 loop) Table Changes in the fluorescence of tryptophan probes inserted in the L1 and L2 loop upon binding of nucleotides and DNA The fluorescence of modified...

Ngày tải lên: 19/02/2014, 06:20

... presence of CI More precisely, the )21 to )48 and )52 to )87 regions of the top strand and )24 to )53 and )58 to )87 regions of the bottom strand were protected by CI (Fig 3B) The centers of these ... CTCGAGCATTTTAACTACGTTTG Synthesis of O1 DNA Synthesis of O2 and O1O2 DNAs Synthesis of O2 DNA Synthesis of O3 DNA Synthesis of O3 DNA Synthesis of S aureus cspC DNA Synthesis of S aureus cspC DNA (Fig ... first base of the start codon of Cro was considered as +1 and the whole sequence was numbered with respect to +1 that the intensities of six bottom strand guanine bases and five top strand guanine...

Ngày tải lên: 07/03/2014, 00:20

... (located upstream of hbpS and ending at the 5¢-end of binding site III) GCCGAATTCCGCCGGACCGGATG (located upstream of hbpS and beginning at the 3¢-end of binding site III) For sequence and characteristics, ... and then cleaved with EcoRI Restricted fragments A and B (to delete site I), C and D (to delete site II), E and F (to delete site III), G and H (to delete the perfect inverted repeat) and I and ... CGAGAATTCGAAAACGAACGGTGC (located upstream of furS and ending at the 5¢-end of binding site I) GTTGAATTCTCGTGTTTATGAGGG (located upstream of furS and beginning at the 3¢-end of binding site I) GGAGCTGCAGCCCACGCGATCGCG...

Ngày tải lên: 07/03/2014, 10:20

... mutants dHAND-Ala-X and dHAND-AlaXZ (Fig 4B) These results indicated that the major phosphorylation sites of dHAND were located within Fig Analysis of the interaction between dHAND and Akt (A) ... indicate the positions of dHAND mutants (B) Coimmunoprecipitation assay of dHAND and Akt AU5-dHAND was immunoprecipitated from the cell lysate of 293T cells expressing AU5-dHAND and HA-Akt These samples ... first analyzed the effect of phosphorylation on the transcriptional activity of dHAND using reporter assay (Fig 6A) AU5dHAND, -dHAND-Ala-X, -dHAND-Ala, and -E47 were dHAND phosphorylation by Akt...

Ngày tải lên: 07/03/2014, 16:20

... The polarity of triangle indicates the direction of the transcription OL and OH represent the origin of L and H strand replication, respectively LSS, L strand start site; HSS, H strand start site ... downstream of the 16 S rRNA gene, and at the 5¢ end of the tRNALeu gene on mouse mt genome [15,16] Studies on S1 nuclease analysis, coupled with the 5¢- and 3¢-end mapping of D-loop H-strand coded ... representation of the mouse mt D-loop region encompassing the conserved sequence boxes I-III (CSB), tRNA genes, L and H strand promoters (LSP and HSP), L and H strand transcription start sites (LSS and...

Ngày tải lên: 08/03/2014, 08:20

... of Phk-2 in the hemolymph of DCV infected flies (A) MALDI-TOF mass spectrometry analysis of the hemolymph of single flies days after injection of buffer (Tris) or a DCV suspension The position of ... sectioned and counterstained with lead citrate and uranyl acetate MALDI-TOF MS analysis For mass spectrometry analysis, hemolymph of DCV-, or buffer-injected Drosophila was collected and directly ... MALDI-TOF mass spectrometry analysis of dissected ejaculatory bulb (G) and legs (H) showing expression of a 12.8 kDa molecule (arrow) (I) Fluorescent (hindgut) and nonfluorescent (midgut) parts of...

Ngày tải lên: 08/03/2014, 08:20

... physicochemical nature of the binding between laminin and laminin-binding protein (LBP) of Leishmania, believed to be important for the homing of the parasites We investigated the influence of pH and various ... and EDTA Data represent mean of three separate experiments remove free metal, was found to contain 9.84 ± 1.51 nmol of Zn2+ per mol of laminin A small amount of Zn2+, 1.21 ± 0.32 nmolÆmol)1 of ... major cell binding and cell migration site of laminin [22,23] The second one, RNIAEIIKDI, a component of B2 chain of laminin, is associated with the promotion of neurite outgrowth and cell binding...

Ngày tải lên: 08/03/2014, 23:20

... failure of a control RNA (i.e the antisense strand to BcBL1) to bind to any concentration of PyrR tested (Fig 2A) Binding of PyrR to BcBL2 and BcBL3 in standard binding buffer in the absence of effectors ... Fig Analysis of the binding of 32P-labeled BcBL2 to PyrR by the electrophoretic gel mobility shift method in the absence of effector (A) and in the presence of 500 lM GMP (B) The concentration of ... regardless of structure, whereas the specificity of purine nucleotide effects on RNA binding indicated that both the exocyclic oxo and amino groups of the purine ring and the 2¢-hydroxyl group of ribose...

Ngày tải lên: 16/03/2014, 06:20

... composed of unpaired strands, CNBP, hnRNP K, and additional factors [7] Modulation of the concentrations and ⁄ or the biochemical activities of either CNBP or hnRNP K could influence the ability of ... single-stranded nucleic acids and is able to promote interstrand reannealing of DNA as well as RNA [35,36] hnRNP Al is phosphorylated by CK2 and PKA PKA phosphorylation suppresses the ability of hnRNP ... and X is a variable amino acid) typical of retroviral nucleocapsid proteins and the presence of an glycine ⁄ arginine rich region (RGG) box between the first and second Zn knuckles In silico analysis...

Ngày tải lên: 16/03/2014, 12:20

... effect of DNA on hydrodynamic radius (Rh) of hRad51 hRad51 (1 lM) was incubated with 0, 1, 3, and lM of oligo PUC+ (in the absence of any nucleotide cofactor) followed by the measurement of hydrodynamic ... Disassembly and recruitment of hRad51 and hRad52 proteins shorter and thicker nucleoprotein filaments when ssDNA is added [28] Tumour suppressors BRCA2 and p53 interact with the oligomerization domain of ... was incubated in binding buffer in the absence (lanes and 3) or presence of 75 lM ssDNA (lanes and 5) and mM ATP (lanes and 5) for h at 37 °C and then subjected to partial digestion with trypsin...

Ngày tải lên: 16/03/2014, 14:20

... was completed, the intensities of the bands corresponding to single strands of DNA and interstrand cross-linked duplex were quantified Rearrangement of intrastrand cross-links The platinated oligodeoxyribonucleotide ... intrastrand CL of transplatin with their complementary DNA sequences results in a rearrangement of these intrastrand adducts into interstrand CLs [35] The stability of 1,3-GTG intrastrand CLs ... 1,3intrastrand CL of trans-Pt-HMP, 12% of the 1,3intrastrand CLs were transformed into the interstrand CLs Importantly, the yields of these rearrangement reactions involving the 1,3-intrastrand CLs of parent...

Ngày tải lên: 16/03/2014, 14:20