identification reproducibility sensitivity limit of detection and specificity

... between EBNA-1 and BHRF-1 DNA loads and VCA IgG antibody titres Results Performance of EBV QPCR assays: reproducibility, sensitivity, detection limit and specificity The intra-assay and inter-assay ... sensitivity, limit of detection and specificity PCR products were identified by an amplification curve, melt analyses and amplification efficiency generated by the Rotor-Gene™ 6000 Software 1.7 ... EBNA-1 and BHRF-1 DNA targets into plasmid vector pGEM and standard curve construction A novel feature of the assay was the design of a quantification standard incorporating both EBNA-1 and BHRF-1...

Ngày tải lên: 12/08/2014, 01:22

... Assuming a sensitivity of 50% and specificity of 75%, a sample of size 413 subjects would give a 95% confidence interval of plus/minus 0.048% for sensitivity and plus/ minus 0.042% for specificity ... the clinics had a sensitivity of 38.1% and a specificity of 74.5% The positive predictive and the negative predictive diagnostic value were 52% and 62.5% respectively [Table 2] Of those who were ... city and country) which included estimation of haemoglobin, red blood cell count and indices; and white blood cell count both total and differential A peripheral blood smear for assessment of red...

Ngày tải lên: 15/03/2014, 03:20

... unknown specificity, and use SDPprofile to reassign it to one of the specificity groups After this step, most of sequences would probably stay in same groups, but specificity assignment of some ... coinciding specificity Page of 12 apply the standard sensitivity and false positive measures to assess the prediction of SDPs The sensitivity is given by the formula TP/(TP + FN), and the false ... shown in [20], and reflects proximity of two distributions, in our case, the gold standard and the predicted grouping of sequences In the case of generated families we have the standard of truth given...

Ngày tải lên: 12/08/2014, 17:20

... standard methods at SVA, which comprise fermentation of glucose, maltose, lactose, sucrose, starch, mannitol and fructose, and production of lecithinase, tryptophanase, urease and hydrolysis of ... Collection of samples from farms Manure, soil and silage Eleven dairy farms (A-K) from the island of Öland of south-east Sweden were included in this study (Table 1) Blackleg is endemic on this island ... standard method for detection of C chauvoei is based on culture and in most muscle samples from cattle dead from blackleg the amount of C chauvoei is high However, this is not always the case and...

Ngày tải lên: 12/08/2014, 18:22

... characteristic of interest, and suppose that the mean of x is μx and the standard deviation of x is δx Then the control limits of the X control chart are μx ± Lδx where L is defined as the “distance” of ... magnitudes of shift in X and Y, various levels of autocorrelation of X and Y, and correlation between X and Y should be investigated The shift sizes in X and Y are set to 0, 0.5, 1, and Further, ... the First -Detection rate of X, Y% is used to represent the First -Detection 37 rate of the variable Y, and XY% is used to represent the First -Detection rate of both X and Y The First-Detection...

Ngày tải lên: 04/10/2015, 15:45

... endoscopic and radiographic Asymptomatic colonic and gastric carcinoma may present with IDA and exclusion of these conditions is of prime concern The upper endoscopic evaluation should include random ... yield of endoscopy in patients with IDA and to define predictive factors of gastrointestinal (GI) lesions causing IDA and identify clinical and biochemical variables that predict the outcome of ... presence of dyspeptic complaint and its severity calculated by presence of abdominal pain, abdominal pain with hungry and anxiety, abdominal distension, nausea, vomiting, poor appetite and symptoms of...

Ngày tải lên: 25/10/2012, 11:18

... ISOIL for Beads Beating (Nippon Gene, Tokyo) Detection of pathogens and fecal indicators by PCR and qPCR methods The concentration of (c)DNA of Poliovirus and Adenovirus was determined using real-time ... (1996) and Watson and Blackwell (2000) reported The color of supernatant after vortex mixing and centrifugation was brown and that of the concentrate after membrane filtration was yellow, both of ... composition of silt and clay The low recovery rate was also because of the inhibition of nucleic acids extraction and PCR by humic substances extracted from sediment by high pH as Tsai and Olson...

Ngày tải lên: 05/09/2013, 10:15

... higher percentage of heat loss as shown in Figure 16 The emission of carbon monoxide and nitric oxide also limits the use of higher compression ratio The concentrations of NO and CO emission depend ... pressure and temperature are Pu = 0.1 MPa, and Tu = 300K, respectively ul (cm/s) is the burning velocity at Pu and Tu αT And βT as well as ul , are functions of equivalence ratio ( ∅ ), and are ... Journal of Energy and Environment (IJEE), Volume 1, Issue 1, 2010, pp.31-52 41 because, the value of pressure and temperature of the mixture during sparking are higher at higher compression ratios and...

Ngày tải lên: 05/09/2013, 15:28

... Problem of Overweight and Obesity .5 Treatment Guidelines Assessment and Classification of Overweight and Obesity Assessment of Risk Status 11 Evaluation and ... Study of Obesity (NAASO) and the National Heart, Lung, and Blood Institute (NHLBI) It is based on the Clinical Guidelines on the Identification, Evaluation, and Treatment of Overweight and Obesity ... adopted by the Expert Panel on the Identification, Evaluation, and Treatment of Overweight and Obesity in Adults and endorsed by leading organizations of health professionals, are shown in Table...

Ngày tải lên: 14/02/2014, 09:20

... Statistics of the BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the ... also manually compiled and used in a number of hedge scope detection systems, e.g (Kilicoglu and Bergler, 2008), (Rei and Briscoe, 2010), (Velldal et al., 2010), (Kilicoglu and Bergler, 2010), (Zhou ... speculation and negation scopes could be concisely expressed as a 284 combination of lexical and syntactic patterns For ¨ u example, Ozg¨ r and Radev (2009) examined sample BioScope sentences and developed...

Ngày tải lên: 20/02/2014, 04:20

... Proceedings of COLING-04 pp 1367-1373 Geneva, Switzerland Kim, Soo-Min and Eduard Hovy 2005 Automatic Detection of Opinion Bearing Words and Sentences In the Companion Volume of the Proceedings of IJCNLP-05, ... Sources of Opinions with Conditional Random Fields and Extraction Patterns Proceedings of HLT/EMNLP-05 Esuli, Andrea and Fabrizio Sebastiani 2005 Determining the semantic orientation of terms ... restaurants The average numbers of sentences in a review document are 35.49 and 25.89 respectively The purpose of selecting one of electronics products and restaurants as topics of reviews for our study...

Ngày tải lên: 20/02/2014, 12:20

... However, the age and maturity of the child and the preferences of the child and the parents should be taken into account 1.2.2 Identification and classification of overweight and obesity 1.2.2.1 ... rice and pasta • Eat at least five portions of a variety of fruit and vegetables each day, in place of foods higher in fat and calories • Eat a low-fat diet and avoid increasing your fat and/ or ... years by 2010 and to implementing the England and Wales National Service Frameworks for children, young people and maternity services (the Children’s NSFs for England and Wales), and ‘Every child...

Ngày tải lên: 21/02/2014, 11:20

... of 2008 has aroused the interest of U.S policymakers in the mechanisms of corporate governance, compliance, and ethics, and their collective role in preventing and mitigating excesses and scandals ... options back-dating scandals and the mutual fund market-timing scandals of the mid-2000s Of course, the most recent set of corporate scandals has broadly swept across the mortgage and banking sectors, ... sector and other parts of the economy It is in this context that RAND convened a March 5, 2009, conference entitled “Perspectives of Chief Ethics and Compliance Officers on the Detection and Prevention...

Ngày tải lên: 06/03/2014, 22:20

... Members of ISO and IEC maintain registers of currently valid International Standards ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and ... method in the case of particular products The harmonization of test methods cannot be immediate, and for certain groups of products International Standards and/ or national standards may already ... rules for the preparation of products other than milk and milk products, meat and meat products, and fish and fishery products [4] ISO/TR 11133-1, Microbiology of food and animal feeding stuffs...

Ngày tải lên: 07/03/2014, 16:20

... Automatic Correction of Errors In this section we propose our algorithm for automatic correction of errors, which consists out of the following steps: Automatic detection of error candidates, i.e cases ... results different to gold-standard Substitution of the annotation of the error candidates by the annotation proposed by one of the parsers (in our case MSTParser) Parse of the modified corpus with ... detection and correction of errors and compared it to the only other work we have found in this field Our results show that both approaches are rather complementary and find different types of...

Ngày tải lên: 07/03/2014, 22:20

... agreement detection and 4.7% absolute gain on F1 for disagreement detection Table 3: Precision (%), recall (%), and F1 (%) of (dis)agreement detection without sampling, with random downsampling and ... set of prosodic features including pause, duration, and the speech rate of a speaker We also used pitch and energy of the voice Prosodic features were computed on words and phonetic alignment of ... agreement detection, and 1.5% absolute gain on F1 on disagreement detection Table 2: Precision (%), recall (%), and F1 (%) of (dis)agreement detection using manual annotations without and with...

Ngày tải lên: 07/03/2014, 22:20

... semantic type detection, we use binary relations from the training set of the SemEval-2007 competition, all definitions of which share the requirement of the syntactic closeness of the arguments ... hyperonym and hf for xi where hxi xi is the most general hyperonym The same is done for yi Next, we use all negative examples to find Gxi and Gyi which are generalization types of the arguments of a ... presence of a general semantic category of one argument will require a more specific semantic category for the other Generalization per argument is, on the one hand, useful because none of the arguments...

Ngày tải lên: 08/03/2014, 01:20

... analysis of metabolites Reactions were initiated by the addition of 100 ll of a solution of enzyme (200 lgÆmL)1) to 0.9 mL of a solution of 50 lg of substrate in 10% (v/v) dimethyl sulfoxide and incubated ... 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lg of GOU For HP and M, reactions were initiated by addition of 0.1 g of HP or M to 1.0 mL of VM containing 10 lg of GOU After incubation for 12 ... reduced and polymerized form of GOU (DHP-GOU) was synthesized as follows Acetone (50 mL) containing 0.2% GOU aO and 0.2% conyferyl alcohol, and 20 mL of 3% H2O2 were dropped into 430 mL of 100...

Ngày tải lên: 17/03/2014, 03:20

... plantations, and already many of the smallholders rely on prospects of future sales of latex and thus depend on the dynamics of the global market The success story of Yunnan and the high demand of China ... First of all it should give the author an impression of the landscape and land cover, land use practices, distribution of settlements, etc With the collection of GPS points the classification of ... context of research projects of the Centre for Development and Environment (CDE) at the Institute of Geography of the University of Berne The CDE is the executive agency of the NCCR North-South and...

Ngày tải lên: 17/03/2014, 11:20

... (approximately, 20 periods) of the sensor response in the presence of the test gas, and the amplitude of the dc component and of the first four harmonics were extracted FFT coefficients of higher harmonics ... coil in the presence of a gas flow of synthetic air (80% N2 and 20% O2 ) introduced from the bottom of the induction coil The surface of the tungsten pellet was oxidised and at the chosen temperature ... evacuation pipe The WO3 films consisted of nanoparticles with a mean grain size of about nm and a log-normal size distribution with a width of about ±20% of the mean size The deposited films were...

Ngày tải lên: 20/03/2014, 13:04