identification of the target protein

... the mode of annexin II– S100A10 interaction In the case of the latter, the C-terminal domain of S100A10 was proven to be critical for the recognition of annexin [45] However, in the case of the ... its target proteins S100A12 was covalently attached to the surface of the CM5 sensor chip, as described in the Experimental procedures The traces reflect the amount of protein complex formed at the ... at a flow rate of 20 lLÆmin)1 in the presence of mM Ca2+ or mM EGTA Table The affinity kinetics of interaction between S100A12 and its target proteins in the presence of mM CaCl2 The rate constants...

Ngày tải lên: 30/03/2014, 15:20

... dna %19.04 dewohs reed akis citsemod naeroK eht ,PNS 676 eht fo elella G eht rof %55 dna PNS 804 eht fo elella C eht rof %55 dah reed akis citsemod esenihC eht elihW ]6[ reed akis citsemod esenihC ... esenihC ni deifitnedi neeb evah PNRP reed akis eht ni sPNS ehT )1 elbaT( ylevitcepser ,G dna C rof %25.15 dna 84.84 erew msihpromylop taht fo seicneuqerf elella ehT )4 giF( dica cimatulg ot enicylg ... 622 nodoc ta detacol )676 editoelcun ta G → C( PNS eht ,revewoH )1 elbaT( ylevitcepser ,C dna T rof %19.04 dna 90.95 erew msihpromylop taht fo seicneuqerf elella ehT ]6,5[ napaJ dna anihC gnitibahni...

Ngày tải lên: 07/08/2014, 23:22

... range The presence of these highly abundant proteins necessitates the prefractionation of serum samples prior to analysis for low abundant proteins Due to the dynamic insight the analysis of the ... Additionally, the nature of this viral infection supports the secretion of altered proteins into the blood and subsequently the serum due to the propensity of the virus to stimulate apoptosis of infected ... effect on the host and the effect on the virus Finally, many complicating factors can contribute to the altered state of proteins present in the serum, not the least of which is the length of time...

Ngày tải lên: 10/08/2014, 05:21

... indicated above the ends of the horizontal lines Positions of the CArG boxes in the scaffolds are described in Table All of the mapped ESTs were encoded on the complementary strand (in the right to ... effectively regulate the expressions of direct target genes involved in the ripening process Page 10 of 14 We also demonstrated here the direct interaction of RIN with the promoters of the genes involved ... to these CArG boxes Genes in the flanking regions of the RIN-binding CArG boxes and their expression To detect potential target genes of RIN, we analyzed the 5-kb genomic regions flanking the...

Ngày tải lên: 11/08/2014, 11:21

... of the derived predictions for the LacI family applying the Mann-Whitney test to distances of the predicted SDPs to the functional site of the protein compared to all amino acid resudues of the ... based only on the predicted SDPs and ignoring the rest of the alignment Then, for a protein of unknown specificity, it is possible to assign it to one of the specificity groups by the highest PWM ... http://www.almob.org/content/5/1/29 Page of 12 where nj is the size of group j, Σj = 1, ,k nj = N, N is the total number of sequences in the MSA The exact formulae for the expectation value and the variance of MIp are: 20...

Ngày tải lên: 12/08/2014, 17:20

... plasmids are then cloned into the yeast cell and expressed on the cell membrane If the prey protein interacts with the bait protein, this would bind the NubG and Cub domains together to form the ubiquitin ... Interacting Proteins A total of fifteen colonies were selected for the first round of screening Half of the colony was streaked on SD/-Trp agar plate and the other half inoculated into 20µl of PCR ... such as protein ALexA-VP16 (PLV) will activate the expression of reporter genes (LacZ and HIS3) in the nucleus The interaction between various membrane proteins will then be reflected by the activity...

Ngày tải lên: 02/11/2012, 11:08

... [23], a peptide from the Win motif of MLL1 bound to the WD40 repeat protein, WDR5 [24,25] and the C-terminal SET domain in the presence and absence of histone peptides and the cofactor product, S-adenosyl-homocysteine ... observation) In the ternary complex, the histone H3 peptide binds in a deep channel that divides a pair of acidic lobes, one of which is composed of residues from the SET-I region and the other of residues ... structures of other SET domain proteins that also contain the canonical post-SET domain [43–46], formation of the ternary complex results in ordering of the post-SET domain, so that the FEBS Journal...

Ngày tải lên: 16/02/2014, 14:20

... Discussion According to the prion hypothesis, a key molecular event in the pathogenesis of prion diseases is the conversion of the normal cellular form of the prion protein, PrPC, to the disease-associated ... indicative of the existence of stable, partially folded intermediates on the folding pathway of a protein [27–30] The presence of an intermediate on the folding pathway of PrP was further disclosed ... that the folding of PrP follows a three-state mechanism: U M I M N, where U is the protein in the unfolded state, I is the intermediate, and N the native state The examination of the CD spectra of...

Ngày tải lên: 18/02/2014, 17:20

... The ectodomains of both monomers in the dimer are required for this effect, suggesting that the epitope tag of one monomer is masked by the ectodomain of the other monomer in the dimer, and vice ... (Fig 2D) The tryptic cleavage sites were localized throughout the cytoplasmic domains of these four RPTPs One site was conserved in three of the four RPTPs at the )5 position relative to the TyrTrpPromotif ... Taken together, these limited tryptic proteolysis suggest that oxidation induced a change in conformation of the intracellular domain of this panel of RPTPs A functional consequence of the change...

Ngày tải lên: 18/02/2014, 17:20

... complex at the position of the histochemical stain in the left panel Antisera against the SDHC subunit of complex II and against the Rieske protein of complex III revealed the presence of these complexes ... with the sequence of the mature bovine protein the hamster protein has a mitochondrial targeting sequence of 29 residues that is removed, presumably by the metalloprotease in the matrix The mature ... and the effect of the absence of intact complex I, but the results are too preliminary in this regard Heterologous expression of the ESSS-HA subunit The localization of the ESSS subunit in the...

Ngày tải lên: 19/02/2014, 16:20

... On the basis of the results with antibodies, a series of PrP mutants were made to assess whether deletions of certain domains of the protein decrease the SOD-like activity of the protein The ... the conformation of the C terminus indicated that the conformation of the C terminus is important to the activity of the protein Deletion of the whole C terminus rendered the protein inactive ... of the protein [17] The importance of residues 35–45 is currently unknown However, it might be that this region of the protein interacts with other regions of the protein, possibly to bring the...

Ngày tải lên: 21/02/2014, 00:20

... possesses the same characteristics of the interior of the protein matrix The script a is the peak–peak distance between the maximum at %287 nm and the minimum at %283 nm, and the script b is the peak–peak ... Figure shows the dependence of the relative intrinsic fluorescence intensity of PsbQ with the Fig Fluorescence quenching of the native PsbQ protein Stern– Volmer analyses of the quenching of the single ... of the hits The hits 1vltB0 and 1aep00 had a confidence level of >80% They correspond to different proteins of the same CATH [68] family (1.20.120.x, Tables and 2) Although most of the scores of...

Ngày tải lên: 21/02/2014, 00:20

... interpretation of the data suggests that the indicated basic region of ic3 of the D2 receptor and acidic region of the C-tail of the D1 receptor might be involved in the interactions between the two ... co-expressed with the appropriate a subunit of the G protein and not in the other case Although there is discussion in the literature concerning the possibilities of photoconversion of YFP into a ... result from the different localization of these proteins in the cell The molecular mechanisms underlying the transport processes of GPCRs from the ER to the cell surface have recently become the subject...

Ngày tải lên: 07/03/2014, 03:20

... on these sequences, the evolution of the cysteine proteinases within the Caricaceae was investigated Furthermore, proteolytic enzymes were 452 purified from the latex of V · heilbornii and the ... in the higher activity observed in Vasconcellea spp.: (1) a higher protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these ... hypotheses, the protein concentration per milligram of dried latex, as well as the amidase activity per milligram of protein, was measured for three species (Table 1) The results show that the protein...

Ngày tải lên: 07/03/2014, 11:20

... outside this region, the proteins are difficult to align because identity between the two proteins is low Nevertheless, the alignment in Fig shows that the overall similarity of the protein encoded ... subunit of Pph3p required for the binding of Psy2p, we examined the sensitivity of the YBL046w ⁄ Psy4p deletion strain to cisplatin The comparable increased sensitivities to cisplatin of the homozygous ... identical to those of the wild-type cells Examination of the phenotype of S cerevisiae carrying deletions for both the YBL046w gene and the TUB2 gene Since decreased expression of Ppp4c in D melanogaster...

Ngày tải lên: 07/03/2014, 12:20

... final D600 of (± 0.5) A fraction of these cultures (equaling approx 50 mg of wet weight cells) was collected to determine the level of storage carbohydrates after entry of stationary phase The remainder ... homologue of the putative transcription factor Sut1p [11], we reasoned that Sut2p might act to modify the transcription of one or both RAS genes Therefore, we determined the level of total Ras protein ... this report we describe the isolation of SUT2 in a screen for high copy suppressors of the synthetic slow growth phenotype of Dgpa2 Dras2 In addition to SUT2, a number of other suppressors were...

Ngày tải lên: 07/03/2014, 15:20

... suggesting that the level of GFP–PrPC transgene expression was dependent on the colour of the background of the animal The fusion protein was found only in the NIL and not in the AL of black- and ... suggesting that the expression of the GFP–PrPC fusion protein in the intermediate pituitary is coregulated with these proteins The POMC and p24d1 ⁄ protein levels were similar in the ALs of black- ... for the GFP–PrPC fusion protein To examine the effect of the overexpressed Xenopus GFP–PrPC protein on the biosynthesis and processing of POMC as well as the secretion of the POMCderived products,...

Ngày tải lên: 16/03/2014, 14:20

... estimated from Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined ... as indicated in Fig 4B The absence of Mo(V)signals reveals the g2-component of FeSII It is noted that the g-factors of the FeSI and II signals of the Qor proteins from the wild-type strain and ... microwave power of mW Trace B is multiplied by a factor of six to show the small signals of the rapid species in this sample The arrows indicate the position of contribution of the resting species...

Ngày tải lên: 17/03/2014, 10:20

... to that of PMT-C3 is that, at the sequence level, there is no discernible similarity of PMT-C3 to the proteins, with the exception of the Cryseobacterium protein glutaminase However, there is ... superimposable with this group of proteins, but does not belong to either of the TGase or NAT families The crystal structures of another family of bacterial type secretion system effector proteins, called ... is the detection of the modification, which involves the detection of a molecular mass change of Da in a relatively large protein substrate (20–45 kDa) Another challenge is the development of...

Ngày tải lên: 22/03/2014, 15:21

... demonstrating the crucial involvement of the MEK2 C-terminus in the interaction Taken together, these results indicate that the PDZ2 domain of hDlg and the C-terminal RTAV sequence of MEK2 are ... in their target proteins [8] On the basis of site-directed mutagenesis of both interactants, we confirmed the major involvement of the PDZ-2 domain of hDlg in the interaction with the C-terminal ... conferring membrane targeting to the protein The b isoform contains a Lin-2,-7 domain that allows 2660 dimerization or interaction with other partners [24] We found that the two isoforms of hDlg expressed...

Ngày tải lên: 22/03/2014, 16:20