identification of antisense oligonucleotides that inhibit the expression of pp1 y1
Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... worthy of remark that both fragments of esculentin peptide were found to affect the integrity of the microbial plasma membrane and the synthesis of the microbial cell wall To the best of our ... possibility that variations in the production of these proteins account for the killing process of Esc(1–18) Note that only a few studies on the effect(s) of antimicrobial peptides on the proteomes of ... represents the first example of the effects of an antimicrobial peptide from frog skin on the proteome of bacteria, and demonstrates that the bacterial membranes are the major targets of its mechanism of...
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Báo cáo Y học: Amphibian peptides that inhibit neuronal nitric oxide synthase The isolation of lesueurin from the skin secretion of the Australian Stony Creek Frog Litoria lesueuri docx
Ngày tải lên :
31/03/2014, 15:20
... Dimerization of the oxygenase domain is necessary for catalytic activity [39,40] The signi®cant departure of the Hill slope of all of the inhibition curves from unity was the ®rst indication that these ... causing the inhibition of nNOS To con®rm this suspicion and to elucidate the general mechanism of inhibition, an analysis was conducted of the kinetics of the nNOS inhibition reaction at different inhibitor ... must therefore inhibit the formation of nitric oxide by either blocking one or more of the cofactor sites on nNOS or by some chemical modi®cation reaction with nNOS that alters the activity of the...
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Báo cáo y học: "The utility of pathway selective estrogen receptor ligands that inhibit nuclear factor-κB transcriptional activity in models of rheumatoid arthrits" pps
Ngày tải lên :
09/08/2014, 06:22
... lipopolysaccharide induces a cascade of events that leads to the degradation of IκB; activated NF-κB then translocates to the nucleus, where it binds to DNA elements in the promoters of several proinflammatory ... converts the chronic diarrhea that these rats experience to a normal stool [27] If the disease is allowed to progress, they begin to show symptoms of arthritis In these settings, treatment of WAY-169916 ... from these rats In the spleen, 36 genes were identified that were induced twofold by adjuvant treatment (average fold change; Table 3) Of those 36 genes, WAY-169916 decreased the expression of...
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Báo cáo y học: "Identification of intra-group, inter-individual, and gene-specific variances in mRNA expression profiles in the rheumatoid arthritis synovial membrane" pot
Ngày tải lên :
09/08/2014, 10:23
... addition, the results of the statistical tests were influenced by The variance-fold is defined as the quotient of the variance of one group (for example, OA patients) and the variance of another group ... in the design and coordination of the study, including supervision of the bioinformatic analyses RWK contributed to the design and coordination of the study and participated in the writing of the ... cycle sequencing of the PCR products The P value can be obtained by calculating the value of the cumulative distribution function at the point F This is equivalent to the integral of the probability...
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Báo cáo y học: " Identification of functional modules that correlate with phenotypic difference: the influence of network topology" potx
Ngày tải lên :
09/08/2014, 20:21
... of 16 functional form of Ψ by assuming that the influence of genes i and j on one another will drop as the ratio of the shortest distance between them to their correlation, the latter being obtained ... in Figure S1 in Additional file We further define the TIF of a gene i as the average mutual influence that the gene imposes on the rest of the genes in the pathway In particular (see Materials ... interpretation of expression data, the pathways reported in PWEA give insights into the nature of the different types of cancer that were examined One of the potential problems with the method presented...
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Báo cáo y học: " Identification of NCAM that interacts with the PHE-CoV spike protein" pdf
Ngày tải lên :
12/08/2014, 01:22
... Approximately 38% of the phage clones had an insert size of 830 bp (B) 25% of the phage clones had an insert size of 750 bp (C) 22% of the phage clones had an insert size of 400 bp (D) 15% of the phage ... of virus at the plasma membrane of a susceptible cell that in turn may enhance the infectivity of the virus by facilitating the interactions of the envelope protein with a cellular receptor that ... insert size of 750 bp, 22% had an insert size of 400 bp and 15% had an insert size of 250 bp (Fig 2) The clones were then further sequenced DNA sequences of the inserts from the fifth round of panning...
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báo cáo khoa học: " Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis" pps
Ngày tải lên :
12/08/2014, 05:20
... Figure Verification of microarray gene expression data by RT-PCR Verification of microarray gene expression data by RT-PCR The expression of three genes selected for further GUS expression assays ... Table shows the results for four representative lines for each construct These results clearly demonstrate that gametophytic expression of the full-length TIO cDNA under the control of MSP1 and ... DR and RB gratefully acknowledge the financial support from the Grant Agency of the Czech Republic (grant 522/06/0896) and from the Ministry of Education of the Czech Republic (grant LC06004)...
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báo cáo khoa học: " EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility" pptx
Ngày tải lên :
12/08/2014, 05:20
... accessions of wild relatives (1 accession of C myriocarpus, of C metuliferus, of C africanus, of C zeyheri, of C dipsaceus, of C prophetarum, of C meeusei, of C anguria, of C ficifolius and of Cucumis ... with the DNA of the reference genotype (cultivar Védrantais) A black arrow on the top left of each image indicates the position of homoduplex DNA Arrows on the right of each panel indicate the ... EcoTILLING allows the approximate location of the polymorphism within the locus studied and, therefore, restricts the necessity of sequencing the complete locus but only regions around the polymorphism...
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Báo cáo y học: "Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals" ppt
Ngày tải lên :
12/08/2014, 23:22
... http://www.retrovirology.com/content/6/1/107 Figure Characterization of Env-Ory1 Characterization of Env-Ory1 (A) Schematic representation of the Env-Ory1-associated ERV with the LTRs and the splice sites for the sub-genomic env transcript ... ¡ ¡ ( teristic profile of a bona fide syncytin gene, with high levels of expression in the placenta and very limited expression in other tissues Expression in the placenta decreases with ... analysis of Env-Ory1encoding transcripts in the placenta, using appropriate primers (see Methods and Figure 3) Analysis of the amino-acid sequence of Env-Ory1 (consensus in Figure of the four...
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Báo cáo y học: "Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites" pdf
Ngày tải lên :
13/08/2014, 09:21
... rather depends on the nature of the target Blocking expression of HIV-1 in vivo To evaluate the capacity of the antisense LNA to inhibit cellular HIV-1 expression the expression of the viral Gag ... suggesting that annealing of the LNA antisense to the single stranded loop of the DIS hairpin is very effective and sufficient to block dimerization The inhibitory potential of the antisense oligos ... essential step in the viral replication cycle, and antisense oligonucleotides may inhibit this step We therefore tested the ability of the four LNA antisense oligonucleotides to inhibit reverse...
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Báo cáo sinh học: "Induction of revertant fibres in the mdx mouse using antisense oligonucleotides" pptx
Ngày tải lên :
14/08/2014, 19:22
... 6) Due to either its size, the quality of the RNA, or the efficiency of the cDNA synthesis and amplification, the full length product of 5720 bp was not generated by this assay and the reaction ... involve the loss of up to 66% of the dystrophin gene [33] It would appear that most exons encoding the rod domain may be deleted without substantial loss of function The loss of a single exon that ... Lipofectin (Life Technologies) at the ratio of 2:1 lipofectin:AO and used in a final transfection volume of 500 µl/well of a 24-well plate as per the manufacturer's instructions, except that the...
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Báo cáo y học: "Identification of motifs that function in the splicing of non-canonical introns" doc
Ngày tải lên :
14/08/2014, 20:22
... Together, these data suggest that the compositional biases seen in the region upstream of the PY tract correlate with the potential for U2AF65 binding, especially for GC-rich introns, and that the ... suggesting that the presence of the mutant AAA sequence in that region of LCAT intron does not act as an ISS These results suggest that GRM1 and GRM2 are ISEs important for the splicing of LCAT ... eliminates the role of the C-rich motifs We next investigated the role of the PY tract in LCAT intron splicing We mutated the PY tract to determine whether the C-rich sequences in the PY tract...
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Báo cáo y học: " Identification of transcripts with enriched expression in the developing and adult'''' pptx
Ngày tải lên :
14/08/2014, 20:22
... dividing the ratio of the tag count in the library of interest versus its mean count in all the other libraries, multiplied by the log of its count in the library of interest, by the number of libraries ... and this reflects the specialized nature of these cells Cluster 14 had the lowest median S and the flattest expression profile of the clusters In sum, these data suggested that the clusters represented ... images of the area of the embryo containing the pancreas were obtained and the pancreas outlined The brightness and contrast of some of the images were altered using Photoshop to better assess the...
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IDENTIFICATION OF A MINIMAL CIS-ELEMENT AND COGNATE TRANS-FACTORS REQUIRED FOR THE REGULATION OF RAC2 GENE EXPRESSION DURING K562 CELL DIFFERENTIATION
Ngày tải lên :
24/08/2014, 12:22
... kinase and helicase activity The kinase activity of this factor is required for the phosphorylation of the C-terminus domain (CTD) of Pol II that consists of repeats of the amino acid sequence Y-S-P-T-S-P-S ... in the promoter-specific activation of genes Histone acetyltransferases are the group of enzymes that catalyze the transfer of an acetyl moiety from acetyl coenzyme A to the ε-NH3+ groups of the ... regulation of genes Genomic DNA is the ultimate template of our heredity In spite of the completion of the human genome project, many challenges remain in understanding the regulation of genetic...
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Identification and characterization of a candida albicans alpha 1,2 mannosyltransferase CaMNN5 that suppresses the iron dependent growth defect of saccharomyces cerevisiae aft1 delta mutant
Ngày tải lên :
16/09/2015, 08:30
... this pathogen However, the mechanisms of transcriptional regulation of these systems are largely unknown The aim of the present study is to identify the functional counterpart of AFT1 in C albicans, ... 2-mannosyltransferases that catalyze the addition of the second and third mannose residues in the O-linked mannans Deletion of both copies of MNT1 and MNT2 resulted in decrease in the level of in vitro ... showed that the application of 10% to 20% serum was sufficient to inhibit the growth of C albicans in growth media And this inhibitory activity can be counteracted only by the addition of iron...
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Báo cáo y học: "Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene"
Ngày tải lên :
02/11/2012, 10:14
... which they were isolated had an inhibitory effect [7] The number of mature liver schizonts increased in the presence of mAbs anti-CSP of P yoelii and the authors suggested that enhancement of infection ... observations the authors suggested that malaria sporozoites are able to control the protein synthesis machinery in cells of the vertebrate host, which could explain the differences in the levels of expression ... erythrocytic [7] cycle However, there is almost no information on the effect of antibodies on the expression of Plasmodium immunogenic molecules There are evidences that antibodies can interfere...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
Ngày tải lên :
16/02/2014, 09:20
... molecules of the asymmetric unit of the wild-type structure Neither the mutation nor the different crystallization conditions evoked structural differences The crystal was grown in the presence of phytate, ... well The hydrogen bond between the backbone nitrogen atom of Asp291 and the 3phosphate of the substrate is the only interaction with the protein backbone; all other contacts are formed using the ... These are not part of the predicted binding mode of phytate to PhyK Out of the water molecules included in the structure none is bound in the catalytic cleft However, it cannot be ruled out that...
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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
Ngày tải lên :
18/02/2014, 04:20
... induce inhibition of proliferation of VSMC, which is mechanistically linked to a KLF4-induced enhancement of the expression of the tumour suppressor gene p53 [5] Because of the important roles of ... we found that the promoter of SR-BI contained multiple putative KLF4 binding sites However, the direct effect of KLF4 on the expression of SR-BI remains unknown Here, the expression of KLF4 in ... demonstrated the increasing expression of KLF4 in PBMC and THP-1 cells, and identified that induction of KLF4 by HDL3 promoted the expression of hSR-BI It has been shown that disruption of SR-BI in...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
Ngày tải lên :
18/02/2014, 08:20
... into the chitinolytic potential of L lactis, the present results provide only the third example of the role of family 33 CBPs in the degradation of recalcitrant polysaccharides Furthermore, the ... course of the degradation of (GlcNAc)3 (r) and (GlcNAc)4 ( ) by LlChi18A, illustrated by the production of (GlcNAc)2 during the initial linear phase of the degradation reaction Note that the enzyme ... Although the occurrence of family 33 CBPs has been known for some time [23], the present results provide only the third demonstration of the accessory function of these proteins The effect of LlCBP33A...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
Ngày tải lên :
19/02/2014, 06:20
... have shown that the expression levels of HO-2 protein were transiently decreased in the mouse liver after days of normobaric hypoxia [26] These results suggest that the reduced expression of HO-2 ... together, these results indicate that hypoxia reduces the expression levels of HO-2 mRNA and protein in five out of six cell lines, with the exception of KG1 myeloid cells A Hypoxia decreases the ... Effects of hypoxia on HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme...
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