identification of a second pth receptor

... Ishikawa, Y., Samejima, T and Tajima, M Isolation and serial propagation of porcine epidemic diarrhea virus infection in cell cultures and partial characterization of the isolate J Vet Med Sci ... D and Rott, R Cotranslational and posttranslational processing of viral glycoproteins Curr Top Microbiol Immunol 1980, 90, 19-48 21 Kusanagi, K., Kuwahara, H., Katoh, T., Nunoya, T., Ishikawa, ... reagents, Amersham, USA) was added Developing was performed on the ECL film (Amersham, USA) As a control, VOPBA of TGEV was performed in porcine enterocytes using the same method as VOPBA of...

Ngày tải lên: 07/08/2014, 17:22

... CTTGGC GCCAGCAG ATACCA GGGAC TAGGA 39 GCCTGGAGTGT C C CAGGG CTAGG NA XXXXXXAGT CAT CAGGG ATTGGG 16 GCCAGCA CCCT GACAGGG CTTGGA 6E4 GCCAGCAGTTT TCT 40 GCCAGCAGTTTA B/22 GCCAGCAGT C A .ACAGGG ... .CAGCCCCAGXX X .CAGCCCCAGCA T TTCACCCCT CCAC TCAGCCCCAGXX X TCAGCCCCAGXX X TCAGCCCCAGCA T .CAGCCCCAGCA T .CAGCCCCAGCA T .CAGCCCCAGCA T TCAGCCCCAGCA T CTATGGCTACXXX TCAGCCCCAGCA T not available tions 105, ... GCCAGCAG CCA .ACAGGGG CTCGG B/9 GCCAGCAGTTT TCA GGGAC TCGG aNucleotides 5' J region C GGG TTGGG CAGCCCCAGCA T .TATGGCTACACC .CAGCCCCAGCA T .CAGCCCCAGCA T .CAGCCCCAGXX X .CAGCCCCAGXX X .CAGCCCCAGCA...

Ngày tải lên: 18/06/2014, 15:20

... GGGACAAGTG AAAATGTCTC TTCAGGGGAT TCAGCTTAGT GACTTGTCCT ATAAGCATGC AATCCTTAAA GAATCACAGT ACACAATCAA GGGACAAGTC AAAATGTCTC TTGAAAGTAT TAGACTCAGT GACTTGGAGT ACAAACATGC AATTCTTGAA GACTCTCAGT ATACAATTAG ... aMPV /A/ UK/3b AGGTAGTGAG GTGCAGGCAG TATTGACCAA GACA TACT CTCTTGG-GA AGGGCAAAAA CAGCAAAGGG GAGGAGTTGC AAATGTTAGA CGGTAGTGAA GTACAGGGTG TTATGACCAA GATTGTTACA CTTTCGGCAG AGGGTTCTGT CAGAAAGCGA GAGGTGCT AAACATTCAC ... CAATGGGAGC AATGG TTA GGGAAAAAGT GCAACTCA 444 443 aMPV/C/US/Co aMPV /A/ UK/3b ACCAATACCA CAAAATCAAA GACCATCATC CCCGGATGCT CCTATCATAC TACTCTGCAT AGGAGCATTA ATCTTCACGA AGCTGGCATC CAA - -AGAATCAAA...

Ngày tải lên: 19/06/2014, 08:20

... GGGACAAGTG AAAATGTCTC TTCAGGGGAT TCAGCTTAGT GACTTGTCCT ATAAGCATGC AATCCTTAAA GAATCACAGT ACACAATCAA GGGACAAGTC AAAATGTCTC TTGAAAGTAT TAGACTCAGT GACTTGGAGT ACAAACATGC AATTCTTGAA GACTCTCAGT ATACAATTAG ... aMPV /A/ UK/3b AGGTAGTGAG GTGCAGGCAG TATTGACCAA GACA TACT CTCTTGG-GA AGGGCAAAAA CAGCAAAGGG GAGGAGTTGC AAATGTTAGA CGGTAGTGAA GTACAGGGTG TTATGACCAA GATTGTTACA CTTTCGGCAG AGGGTTCTGT CAGAAAGCGA GAGGTGCT AAACATTCAC ... CAATGGGAGC AATGG TTA GGGAAAAAGT GCAACTCA 444 443 aMPV/C/US/Co aMPV /A/ UK/3b ACCAATACCA CAAAATCAAA GACCATCATC CCCGGATGCT CCTATCATAC TACTCTGCAT AGGAGCATTA ATCTTCACGA AGCTGGCATC CAA - -AGAATCAAA...

Ngày tải lên: 20/06/2014, 04:20

... glycoprotein a1 -Acid glycoprotein Fetuin Galb1-4GlcNAc-Ra NeuAca2-6Galb1-4GlcNAc-R NeuAca2-3Galb1-3GalNAca1-O-Ser/Thrc NeuAca2-3Galb1-3[Neu5Aca2-6]GalNAca1-O-Ser/Thrc NeuAca2-6(3)Galb1-4GlcNAc-Rc Galb1-3GalNAca1-O-Ser/Thr ... two specific primers For 6I 5¢-CGATGAATTC GTTAACGCTCATCACCATCACCATCACGGGAAA TTGGCCATGGGGT-3¢ containing a HpaI site and Back 6I 5¢-CGATGGTACCGTACTTGTTCATGCTTAGG-3¢ and subcloned into pUC19 for further ... Galb1-3GalNAca1-O-Ser/Thr Galb1-4GlcNAc-R Gala1-O-pNp GalNAca1-O-pNp GlcNAca1-O-pNp Galb1-4GlcNAcb-O-octyl Galb1-3GlcNAcb-O-octyl Galb1-3GalNAca1-O-bn Galb1-4GlcNAc Galb1-3GlcNAc LNnT: Galb1-4GlcNAcb1-3Galb1-4Glc...

Ngày tải lên: 08/03/2014, 08:20

... (forward, 5¢-AAG GCT TAC CAC GAG CAG CTA TCA-3¢; reverse, 5¢-ACA GGC CAT GTA CTT TCC GTG TCT-3¢) and tobacco (forward, 5¢-ATG AGA GAG TGC ATA TCG AT-3¢; reverse, 5¢-TTC ACT GAA GGT GTT GAA-3¢) a- tubulinspecific ... ATG start codon and SacI downstream of the TAA stop codon for easy cloning in the forward and reverse primers, respectively (forward, 5¢-ctc gag ATG AAG AGA ACA CAT TTG GCA-3¢; reverse, 5¢-gag ... plants, the average starting quantity of AtZFP11 was normalized to the average starting quantity of the a- tubulin gene, which is assumed to be at a constant levels in all the samples The Arabidopsis...

Ngày tải lên: 16/03/2014, 06:20

... for translation initiation, 5¢-(C ⁄ A) AA (A ⁄ C)ATG, and a putative poly (A) addition signal sequence, 5¢-AATAAA [17,18] It encoded a predicted product of 255 amino acids with a molecular mass of ... may be an artificial event Association of DmPCNA2 with Drosophila DNA polymerases d and e PCNA was originally identified as a DNA sliding clamp for DNA polymerases [22] In humans, PCNA associates ... reagents (Amersham Pharmacia Biotech, Piscataway, NJ) Animals were fed water and standard rabbit food and maintained on a 12 h light/dark cycle Polyclonal antiserum to the peptide was raised in rabbits...

Ngày tải lên: 23/03/2014, 10:20

... that are increased as well as those that are decreased in the plasma of patients displaying clinical symptoms of pancreatic cancer In this study, we assembled a cohort of 52 cases of locally advanced, ... Figure CEA and CA125 are significantly prognostic for advanced, unresectable pancreatic cancer CEA and CA125 are significantly prognostic for advanced, unresectable pancreatic cancer (A) Kaplan-Meier ... unresectable, advanced pancreatic cancer as well as a cohort of 43 healthy, age-matched volunteers After calculating log2 PLA units for each tumor marker within each sample (Materials and Methods),...

Ngày tải lên: 18/06/2014, 15:20

... ligase (Roche, Mannheim, Germany) The first amplification stage (20 cycles, 50 μl reaction volumes) used 300 nM of primers CTCGTAGACTGCGTACGATG and GACGATGAGTACTGATCGC at 56°C annealing temperature ... Microarray-based detection and genotyping of viral pathogens Proc Natl Acad Sci U S A 2002, 99:15687-15692 Allander T, Andreasson K, Gupta S, Bjerkner A, Bogdanovic G, Persson MA, Dalianis T, Ramqvist ... Isolation and identification of a novel human parechovirus J Gen Virol 2004, 85:391-398 Abed Y, Boivin G: Molecular characterization of a Canadian human parechovirus (HPeV)-3 isolate and its relationship...

Ngày tải lên: 20/06/2014, 01:20

... primer, ABV3RaceInner, 5'-GCAATCCAGGAATAAGCAAGCACAAA-3' (maps to residues 8595-8620 of vRNA) Both of the RACE PCR reactions were carried out with Platinum Taq polymerase (Invitrogen, Carlsbad, CA) ... primer ABV3RaceOuter, 5'-CCCGTCTACTGTTCTTTCGCCG-3' (maps to residues 8479-8497 of vRNA), followed by inner PCR using Tailed_RNAligateRC, 5'AAGCAGTGGTAACAACGCAGAGTACGGGTGAAAGTGATAAC-3' and the ... sequences: ABV1r, 5'-ATGACCAGGACGAGGAGATG-3' (maps to residues 8831-8812 of vRNA), ABV2r, 5'-CCTGTGAATGTCTCGTTTCTG-3' (maps to residues 5754-5733 of vRNA), and ABV3r 5-TTCTTTCAGCAACCACTGACG-3' (maps...

Ngày tải lên: 20/06/2014, 01:20

... primer pairs from Integrated DNA Technologies: Muc16 5'-TGCCACCTACCAGTTGAAAG-3' and 5'-GTACCGCCAAGCAGATGAG-3'; GAPDH 5'-TGCTGAGTATGTCGTGGAGTCTA-3' and 5'AGTGGGAGTTGCTGTTGAAGTCG-3' The amplified ... murine monoclonal antibodies that were initially generated against human MUC16 List of abbreviations EOC: epithelial ovarian cancer; ConA: Concanavalin A; αMe-Man: α-methylmannopyranoside Competing ... binding is a high affinity, N-glycan dependent interaction that facilitates peritoneal metastasis of ovarian tumors Mol Cancer 2006, 5:50 Patankar MS, et al.: Potent suppression of natural killer...

Ngày tải lên: 20/06/2014, 07:20

... interpretation, and modification of the initial version of this study Author details Department of Differential Equation, Institute of Mathematics and Mechanics-Azerbaijan National Academy of Science, ... equations Nauk Dumka, Kiev, 284 (1984) (Russian) 31 Yakubov, S, Yakubov, Ya: Differential-Operator Equations Ordinary and Partial Differential Equations Chapman and Hall/ CRC, Boca Raton, 568 ... regularized trace of the considered problem will be studied It is clear that because of the appearance of an eigenvalue parameter in the boundary condition at the end point, the operator associated...

Ngày tải lên: 21/06/2014, 02:20

... AAAACTGAACAAAAGAC3') together with one of the reverse primers S1OLIGO3' (CTAAACTAACATAAGGG C3') or degenerate3'-2 (5'CCATAAGTAACATAAGGG CAA3') [14,16] The RT reaction for synthesis of cDNA consisted ... QIAamp MinElute Virus Spin Kit (QIAGEN, USA) Amplification of the S1 gene by RT-PCR was performed using one of the forward primers S1OLIGO5' (5'TGA AAACTGAACAAAAGA3') or NEWS1OLIGO5' (5'TG AAAACTGAACAAAAGAC3') ... - Arkansas KM91‡ KM91 KM91 KM91 KM91 Variant KM91 Variant Variant Variant Variant GenBank accession numbers AY790368 AY790359 AY790360 AY790358 AY790362 AY790361 AY790366 AY790365 AY790363 AY790364...

Ngày tải lên: 07/08/2014, 20:23

... isolation and identification of a potential viral pathogen was pursued Canine coronavirus was first isolated from a case of canine enteritis during an epizootic in Germany in 1971 Later, Woods and ... sequence was analyzed by GENETYX version 9.0 computer software (Software Development, Japan) and DNAMAN version 4.0 (Lynnon BioSoft, Canada) Bacteriology failed to grow organisms in cultures inoculated ... total RNA was reverse transcribed to cDNA using avian myeloblastosis virus reverse transcriptase (TaKaRa, Japan) and oligo (dT) primers per the manufacturer’s recommendation The PCR detection was...

Ngày tải lên: 07/08/2014, 23:22

... years, and a half-sister (from the paternal side) died of bronchioloalveolar carcinoma at the age of 25 years The patient's grandparents died of different causes, but none had cancer These data ... histological features of lobular carcinoma and infiltrating ductal carcinoma The family history suggested LFS: the patient's father was diagnosed with dorsal soft tissue leiomyosarcoma at the age of ... GCA TTC TGG GAC 120 AGC CAA GTC TGT GAC TTG CAC GTA CTC CCC TGC CCT CAA CAA GAT 135 GTT TTG CCA ACT GGC CAA GAC CTG CCC TGT GCA GCT GTG GGT TGA 150 Figure DNA sequence showing the insertion and...

Ngày tải lên: 09/08/2014, 04:21

... epitopes assay Assay performance characteristics of the anti-SmD3 peptide (SMP) assay (a) Intra-assay and interassay variability, (b) linearity, and (c) receiver operating characteristic analysis ... http://arthritis-research.com/content/7/1/R19 (ANA) profile (Pharmacia Diagnostics; cutoff ratio 1) The latter assay contains the autoantigens U1-68 kDa, U1 -A, U1-C, SmBB', SmD, Ro-52, Ro-60 and La All ELISAs were performed in accordance ... peptide-based assay (99.8%; data not shown) Further studies are in progress to compare the assay performance of the anti-SMP assay with that of other commercially available anti-Sm immunoassays For...

Ngày tải lên: 09/08/2014, 06:22

... SLRP degradation may represent an early critical event in the process of cartilage degradation Awareness of the SLRP degradation products may assist in early detection of OA cartilage degradation ... relevant extract of matrix proteins MMP-13 degrades biglycan and decorin Biglycan in human normal and OA cartilage migrated as a doublet at 48 and 45 kDa, representing intact and amino-terminally ... synthesis of dermatan sulphate proteoglycans by fetal and adult human articular cartilage Biochem J 1989, 261:501-508 Imai K, Hiramatsu A, Fukushima D, Pierschbacher MD, Okada Y: Degradation of decorin...

Ngày tải lên: 09/08/2014, 07:20

... 3'-TCC AGG GCT ATG AAT CAC AAC AGG c-Fos 5'-CAG GAG ACA GAC CAA CTA GA 3'-TTC ACG GAC AGA TAA GGT CC DNAX-activation protein 12 5'-ATG GGG GGA CTT GAA CCC Fc receptor γ chain 5'-TGA TTC CAG CAG TGG ... GTC ATC CCT 3'-AGA CAC ATT GAC CAC AGA GGC ACT β-Actin 5'-GTC CTC TCC CAA GTC CAC ACA 3'-CTG GTC TCA AGT CAG TGT ACA GGT AA GAPDH 5'-TGA TGA CAT CAA GAA GGT GGT GAA G 3'-TCC TTG GAG GCC ATG TGG ... CTC AGA 3'-CAG AGC GCT GAT TGG TCC ATC TTA 3'-TCC CGT TGC AGA CGT AGA AAC TGA Integrin αv 5'-TCC CAT CAG TGG TTT GGA GCA TCT 3'-TCC GAC AGC CAC AGA ATA ACC CAA Integrin β3 5'-TGC CTC AAC AAT GAG...

Ngày tải lên: 09/08/2014, 08:22

... collection and assembly of data, data analysis and interpretation, manuscript writing and final approval of manuscript for publication YJ Provided study materials, collection of data, data analysis and ... data, data analysis and interpretation, manuscript writing and final approval of the manuscript XH Participated in the collection and assembly of data, data analysis and interpretation, manuscript ... amplify the truncated form of MsrA and introduce a BamHI cutting site at the 3’ end (MsrA-for: 5’-cctggctgcggaggtggagaaac and MsrA-BamHI-rev: 5’-tggggccaaggatccgctttgaaagaacc) The amplicons were...

Ngày tải lên: 10/08/2014, 05:21

... Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library Xiangan Tu1*§, Jintao Zhuang1*, Wenwei Wang1, Liang Zhao1, Liangyun Zhao1, Jiquan Zhao1, Chunhua ... potential for use in early diagnosis and targeted therapy of RCC Materials Renal carcinoma line A4 98 and a normal renal cell line HK-2 were obtained from Medical Academy of China (Beijing, PR China) ... cG250 and low dose subcutaneous IL-2 in patients with advanced renal cell carcinoma Cancer Immun, 2007, 7: 13 14 Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu Q, Marasco...

Ngày tải lên: 10/08/2014, 10:21