i m in charge dna binding proteins
Báo cáo y học: " A balanced transcription between telomerase and the telomeric DNA-binding proteins TRF1, TRF2 and Pot1 in resting, activated, HTLV-1-transformed and Tax-expressing human T lymphocytes" pot
Ngày tải lên :
13/08/2014, 09:21
... cultivated in RPMI 1640 (Invitrogen) with 10% heat-inactivated fetal calf serum (FCS) 100 U/ml recombinant human IL-2 (rhIL-2) and supplemented with 100 IU/ml of penicillin and 50 µg/ml of streptomycin ... was significantly increased in TSP/HAM cells (Fig 4, lower panel) It is plausible that these differences might be linked to their in vitro /in vivo derivation and/or to selection pressures in culture ... implicated in the initiation of the leukemogenic process [35-39] In spite of this low level of telomerase activity, HTLV-1 in vitro infected T cells and Tax-expressing primary T lymphocytes still continue...
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Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx
Ngày tải lên :
08/03/2014, 08:20
... with their different binding specificities, the two terminator sequences seem to bind to distinctly different proteins The D-TERM DNA binding proteins purified by affinity chromatography contain ... further indicate the sequence specificity of protein cross-linking Purification of D-TERM binding proteins With a view to understand the nature of D-TERM DNA binding proteins and their possible role in ... affinity DNA matrix (1 mL) was mixed with 50 mg heparin–agarose purified mouse liver mt protein extract by gentle mixing in loading buffer, containing 10 mM Tris/HCl pH 7.4, 50 mM NaCl, mM dithiothreitol,...
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Báo cáo khoa học: Deoxyribonuclease I footprinting reveals different DNA binding modes of bifunctional platinum complexes potx
Ngày tải lên :
23/03/2014, 10:21
... characterize the DNA binding of a number of small molecules of biological significance [1,6–15], including antitumor cis-diamminedichloroplatinum(II) (cisplatin) (Fig 1B) and its clinically ineffective ... footprinting of cis- or trans-diamminedichloroplatinum (II)-modified DNA J Mol Biol 236, 969–974 Brabec V (2002) DNA modifications by antitumor platinum and ruthenium compounds: their recognition ... formation, identification, and quantitation Biochemistry 24, 707–713 32 Anin MF & Leng M (1990) Distortions induced in double-stranded oligonucleotides by the binding of cis-diamminedichloroplatinum(II)...
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wow i'm in business, a crash course in business basics 2nd (2008)
Ngày tải lên :
18/04/2014, 14:13
... Edition July 2008 Editor lisa Guerin Cover & Book Design susan putney Proofreading susan carlson greene Index songbird indexing Printing delta printing solutions, inc Stim, Richard Wow! I m in ... several suggestions for maintaining and increasing your interest Here are some of them: Delegate administrative activities If some activities—for example, bookkeeping, documentation, or contracts—obstruct ... overtake any small business owner But smart financial strategies—for example, not relying on a single customer, maintaining a revolving line of credit, and having staffing flexibility—can prepare...
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wow i'm in business, a crash course in business basics 2nd (2008)
Ngày tải lên :
18/04/2014, 14:16
... Edition July 2008 Editor lisa Guerin Cover & Book Design susan putney Proofreading susan carlson greene Index songbird indexing Printing delta printing solutions, inc Stim, Richard Wow! I m in ... several suggestions for maintaining and increasing your interest Here are some of them: Delegate administrative activities If some activities—for example, bookkeeping, documentation, or contracts—obstruct ... overtake any small business owner But smart financial strategies—for example, not relying on a single customer, maintaining a revolving line of credit, and having staffing flexibility—can prepare...
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i m in love pdf
Ngày tải lên :
23/06/2014, 00:20
... My supersonic girl, I' m in love No matter what they say, now, I' m in love I it my own way cause I' m in love And I can't keep that song out of my mind Whistling that silly tune all the time ... I can't keep that song out of my mind Whistling that silly tune all the time whistle I' m in love {whistle I' m in love}fade ...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
Ngày tải lên :
19/02/2014, 05:20
... proteins to the recognition elements is affected by DNA cis-platination in a similar way to p53 binding In agreement with the considerable homology between the p53 and p73 DNA- binding (core) domains, ... chromosomal (genomic) DNA [26] Provided that the cisplatin inhibitory effect on p53 sequence-specific DNA binding is linked primarily to the IACs formed within the target sites, the susceptibility ... adducts within the p53 DNA- binding site (p53DBS), resulting in increased p53 binding to the labeled probe The pPGM4 fragment (c) contains cisplatinresistant p53DBS, and its cis-platination did not...
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Tài liệu Báo cáo khoa học: Disulfide bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt
Ngày tải lên :
18/02/2014, 06:20
... disulfide bridge, while maintaining the same binding stoichiometry, induces changes in binding ability, site selectivity and dynamic properties of L-BABP Thus, the study of a recombinant protein ... M, Ferranti P, Longhi R, Bonvin AM & Molinari H (2007) NMRbased modeling and binding studies of a ternary complex between chicken liver bile acid binding protein and bile acids Proteins 69, 177–191 ... binding and dynamics (2008) Identification and functional characterization of the bile acid transport proteins in non-mammalian ileum and mammalian liver Proteins 70, 462–472 26 Marley J, Lu M...
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Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc
Ngày tải lên :
19/02/2014, 06:20
... to examine whether these loops are actually involved in DNA binding Because aromatic residues are involved in the ssDNA binding by bacterial single stranded DNA- binding protein (SSB) and human ... Y Matsuo et al alanine If the aromatic side chain is involved in the interaction with DNA, then its replacement with alanine, a short side chain amino acid residue, should affect the DNA binding ... loop is involved in DNA binding [26,36,37,45] Saturation mutagenesis of the RecA-L2 loop revealed that mutations in the L2 amino acid residues result in recombination defects in vivo [46] In addition,...
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Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx
Ngày tải lên :
19/02/2014, 12:20
... role in general insulin mRNA stability, as PTB has recently been implicated in insulin mRNA stabilization in normoxic conditions [62] Interestingly, we observed that the insulin mRNA PTB binding ... protein binding sites are underlined Both sequences are required for cytoplasmic CSD/PTB complex formation and recombinant CSD protein binding (B) A comparison of CSD protein RNA binding sites is ... probes in a gel shift assay The recombinant protein complexes are indicated (D) PTB binding to VEGF mRNA in vivo was investigated using an RNA immunoprecipitation assay Cytoplasmic RNA/protein complexes...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
Ngày tải lên :
19/02/2014, 16:20
... mass spectra from Ca2 +binding proteins Therefore, immunoblotting was employed to confirm the calsequestrin protein spot identified by ÔStainsAllÕ labelling Immunoblot analysis of key Ca2+ -binding ... deficiency in dystrophin on the expression of Ca2+ -binding proteins The relatively unique combination of the pI value and molecular mass of individual 2D spots can be useful in the initial identification ... (http://www.matrixscience.com) affected in mdx muscle (Fig 4C,D,E) Immunoblotting with mAb VIIID12 to calsequestrin revealed a drastic reduction in this Ca2+ -binding protein (Fig 4F), and this finding agrees with the...
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Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx
Ngày tải lên :
22/02/2014, 04:20
... nonspecific metal binding Specificity of Zn(II) binding by the purified Zn(II) -binding proteins The specificity of Zn(II) binding was also examined for the purified Zn(II) -binding proteins The binding ... Fig Competition of radioactive Zn(II) binding by nonradioactive metals, Zn(II), Mg(II) and Fe(III), to purified E coli zinc -binding proteins Mixtures of the purified Zn(II) -binding proteins were ... Zn(II) -binding proteins In order to identify the nature of proteins showing Zn(II )binding activity, we isolated the major Zn(II) -binding proteins from PVDF membranes and subjected them to N-terminal...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... C-terminal villin headpiece domain, which mediates actin -binding in several proteins, such as villin and dematin [59] Both ABLIM-2 and -3 show high homology with ABLIM-1 ABLIM-1 was originally ... coactivator Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking ... ligand -binding domain Actin -binding protein Targeting sequence Gelsolin Direct or indirect association with the AR Region LBD Classes Role in the cytoplasm AR effect Mechanism ()) Actin filament...
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Báo cáo khoa học: DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli doc
Ngày tải lên :
07/03/2014, 10:20
... containing the complete (I, II, III and II + III; underlined) or deleted (DI, DII, DIII and DII + DIII; dotted lines) binding motifs, or the complete (PIII, marked by >>>
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Báo cáo khóa học: FRET evidence for a conformational change in TFIIB upon TBP-DNA binding pptx
Ngày tải lên :
07/03/2014, 15:20
... either TFIIB or CYIIB were added as shown above the autoradiogram Anti-TFIIB Ig or preimmune serum were included in the binding reaction where indicated The anti-TFIIB Ig was described previously ... fusion protein with ECFP preceding the N-terminus of TFIIB and EYFP following the C-terminus (CYIIB) ECFP-TFIIB was made by inserting the TFIIB gene into pRSETb-YC2.1 via SphI and EcoRI sites ... Solution structure of the C-terminal core domain of human TFIIB: similarity to cyclin A and interaction with TATA -binding protein Cell 82, 857–867 Wu, W.H & Hampsey, M (1999) An activation-specific...
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Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc
Ngày tải lên :
31/03/2014, 07:20
... resulting sigma mutant SASIG1–455R/H in competition EMSAs (Fig 6) The maltose binding portion of the recombinant proteins did not interfere with core or promoter binding (data not shown) As is evident ... in native r70 regions 1.1 and are located in close proximity, with region 1.1 acting as an autoinhibition domain Sigma–core interaction induces a conformational change that unmasks the DNA binding ... HTH-motif [20] in region 4.2 is involved in binding of the )35 promoter element [7] As shown in Fig 7A, two conserved amino acids, E13 and R16, can be identified in all three SASIG proteins within...
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Báo cáo Y học: Interferon-alpha inhibits Stat5 DNA-binding in IL-2 stimulated primary T-lymphocytes doc
Ngày tải lên :
31/03/2014, 15:20
... density of ´ 105 cells per ml in complete MEM (MEM supplemented with 10% heat inactivated AB+ serum, mM glutamine 50 mgámL)1 of streptomycin and 50 mgámL)1 of penicillin) and kept in a humid incubator ... Annexin V staining Redistribution of plasma membrane phosphatidylserine is a marker of apoptosis and was assessed using Annexin V FLUOS and Propidium Iodide staining kit according to the manufacturer's ... the initial wave of IFP-53 GAS binding activity, whereas it severely inhibited the reappearance of bandshifts during the second wave of IL-2 induced binding at 24±48 h (Fig 3A) Furthermore, IFN-a...
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báo cáo hóa học:" Assessing the clinical utility of measuring Insulin-like Growth Factor Binding Proteins in tissues and sera of melanoma patients" potx
Ngày tải lên :
18/06/2014, 15:20
... (IGF1R): Insulin-like Growth Factor-1 receptor; (IGFBP-3): Insulin-like Growth Factor Binding Protein-3; (IGFBP-4): Insulin-like Growth Factor Binding; Protein-4; (MAPK): Mitogen-activated protein kinase; ... the insulin-like growth factor binding proteins and the IGFBP proteases in modulating IGF action Endocrinol Metab Clin North Am 1996, 25(3):591-614 Rajaram S, Baylink DJ, Mohan S: Insulin-like ... paraffin embedded tissue specimens from 96 patients using mouse anti-human IGFBP-3 antibody (R&D Systems Minneapolis, MN), including 59 specimens from primary patients and 37 specimens from patients...
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Báo cáo khoa học: "Immunohistochemical localization of calcium binding proteins and some neurotransmitters in myenteric plexus of goat stomach" doc
Ngày tải lên :
07/08/2014, 18:21
... ytivitcaeronummi PS µ giF m 05 = rab )B( muluciter eht ni tnanimod era srebif RI-RC fo seitisocirav dna ,)D-A( )sdaehworra( srebif eht ni ylno devresbo si ytivitcaeronummi RC musamoba :D ,musamo ... suxelp ciretneym eht ni PS dna PRGC ,RC ,BC fo noitazilacol lacimehcotsihonummi eht ,noisulcnoc nI yteitas langis dna ,atsegni hcamotserof eht fo noitatigruger ,atsegni eht fo gnixim etaitini hcihw ... suxelp ciretneym hcamots eht ni ytivitcaeronummi BC giF 0002TMI aiv erawtfos 0.6 noisrev pohsotohP ebodA gnisu derutpac erew suxelp ciretneym eht ni ytivitcaer -onummi fo segami ehT )mn 095 retlif...
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Báo cáo y học: "Pharmacological disruption of insulin-like growth factor 1 binding to IGF-binding proteins restores anabolic responses in human osteoarthritic chondrocytes" ppt
Ngày tải lên :
09/08/2014, 01:23
... ofto human (IGF)-1 to IGF -binding proteins, on 12 5I- labeledinsulinbinding NBI-31772, a small-molecule inhibitor like growth factor (IGF)-1 to IGF -binding proteins, on 12 5I- labeled IGF-1 binding ... factor binding proteins Arthritis Rheum 1996, 39:968-978 Morales TI: The insulin-like growth factor binding proteins in uncultured human cartilage Increases in insulin-like growth factor binding ... Hardouin S, Binoux M: Analysis of serum insulin-like growth factor binding proteins using Western blotting: use of the method for titration of the binding proteins and competitive binding studies...
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