Tài liệu Báo cáo khóa học: TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei pdf
... broad-spectrum PDE inhibitor IBMX, which is widely used in cell biological experimentation, is essentially inactive on TbPDE1, with an IC 50 value of > 1 m M . Discussion This study reports the identification ... considerably improved by growing the cells in TB medium instead of Luria–Bertani medium (see Materials and methods), low activity and high instability remained unsatisfactory. Inclusion of 5 ... amino acids at its C-terminal side. Expression of TbPDE1 in S. cerevisiae The successful complementation screening in yeast indicated that recombinant TbPDE1 is enzymatically active. In addition...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx
... effective inhibition of binding of ICAM-4 positive red cells by anti-ICAM-4 antibodies, indicate a major role for ICAM-4 in binding of red cells to the I domains. The efficient inhibition of the Fig. ... antibody is involved in ICAM-4 binding. Treatment of the coated CD11b I domain fusion protein with I domain specific anti-CD11b antibodies resulted in an efficient inhibition of the adherence of ... their ability to inhibit in solid phase assay. MEM170, 44a and 107 were highly active inhibitors of the CD11b I domain GST interaction with ICAM 4, whereas LM2/1 inhibited weakly but significantly...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
... the similarity of the t wo fasciclin domains in CLAP with other fasciclin I containing proteins is clear (Fig. 9). Using BLAST (http://www.ncbi.nlm.nih.gov/BLAST) to identify related proteins, ... cell adhesion protein in Artemia (Eur. J. Biochem. 271) 4017 Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS -I family of cell adhesion proteins Alden ... preincubation at 40 °C and pH 6.8, whereas the dimer retained about 70% of its initial activity under these conditions (Fig. 4C). Similar differences in cathepsin L activity were observed at a ll incubation...
Ngày tải lên: 07/03/2014, 16:20
Recombinant DNA I - Basics of molecular cloning Polymerase chain reaction cDNA clones and screening
... becomes available, the uses of PCR expand. • With appropriate primers, one can amplify the desired region from even miniscule amounts of DNA. • Not limited by the distribution of restriction endonuclease ... positive selection for recombinants. Not to scale. Introduction of recombinant DNA into living cells via vectors • Autonomously replicating DNA molecules – (have an origin of replication) • Selectable ... procedures to isolate a gene that represents as little as 1 part in a million of the genetic material in an organism. • DNA from the organism of interest is divided into small pieces that are...
Ngày tải lên: 13/03/2014, 18:39
AMERICAN CONSTITUTIONAL LAW VOLUME I SOURCES OF POWER AND RESTRAINT pptx
... meaning in modern times. Article II gives the “executive Power” to the president but does not define the precise limits thereof. Article III likewise invests the Supreme Court with “judicial ... Doctrine of Abstention Closely akin to exhaustion of remedies is the doctrine of abstention. Whereas the principal application of the exhaustion of remedies doctrine is to bureaucratic deci- sion ... justice in 1801, is generally cred- ited with instituting the practice of issuing an Opinion of the Court, which reflects the views of at least a majority of the justices. It appears that this...
Ngày tải lên: 16/03/2014, 03:20
Báo cáo khoa học: Yeast oxidative stress response Influences of cytosolic thioredoxin peroxidase I and of the mitochondrial functional state pot
Ngày tải lên: 16/03/2014, 14:20
[TO BE PUBLISHED IN THE GAZETTE OF INDIA, EXTRAORDINARY PART-II, SECTION-3, SUB-SECTION (i)] - MINISTRY OF CORPORATE AFFAIRS potx
Ngày tải lên: 29/03/2014, 14:20
Appendix I Statement of Expenditures for Independent Counsel Adams potx
Ngày tải lên: 20/06/2014, 06:20
Appendix I: Status of Treasury’s and OMB’s Progress in Addressing GAO’s Prior Year Recommendations for Preparing the CFS_part1 pptx
Ngày tải lên: 20/06/2014, 08:20
Appendix I: Status of Treasury’s and OMB’s Progress in Addressing GAO’s Prior Year Recommendations for Preparing the CFS_part2 pot
Ngày tải lên: 20/06/2014, 08:20
History of Modern Biotechnology I - Springer
... preparation of a stable form of penicillin and the demonstration of its remark- able antibacterial activity and lack of toxicity in mice. Production of penicillin by the strain of Penicillium notatum ... is methyl-cis-3,4-dimethoxycinnamate [220]. The auto-inhibitor of conidial germi- nation in Colletotrichum graminicola is the secondary metabolite, mycosporine alanine [221]. Germination inhibitors ... ATP is syn- thesized as glucose is oxidized, and germination ensues. Considerable evidence has been obtained indicating that gramicidin S (GS) is an inhibitor of the phase of spore germination...
Ngày tải lên: 08/08/2012, 09:52
Báo cáo y học: "Multivariate explanatory model for sporadic carcinoma of the colon in Dukes’ stages I and IIa"
... 1. 061 Accident and Emergency Service. Huelva. Spain. 2. Department of Family Medicine, Primary Care Division of Seville and CIBER Fisiopatologia Obesidad y Nutrición (CB06/03). Instituto de ... HDL-high density lipoprotein. DISCUSSION We have made an investigation to try to vali- date a multivariate explanatory model of the diagno- sis of SCRC in Dukes’ stages I and IIa using non-conditional ... strength to our observations. Although “bootstrapping” is not a technique of measurement of external validity, it is one of internal validity, which in epidemiological terms is prior to the external...
Ngày tải lên: 03/11/2012, 11:34
The Great Ideas of Psychology Part I - Daniel N. Robinson
... award-winning series “The Brain” and the subsequent nine- part series, “The Mind.” He is past president of two divisions of the American Psychological Association: the division of the history of ... the division of theoretical and philosophical psychology. He is fellow of the American Psychological Association and of the British Psychological Society. Dr. Robinson is also visiting senior ... respected the special vulnerabilities of the mentally disturbed. 2. Identify the interplay of social, religious, scientific and political forces in declaring certain persons to be identified as “troubled”...
Ngày tải lên: 07/11/2012, 14:21
i want one of that
... three times a day I often use mine to make pictures I like to send them to my friends Which gadgets in part A do you own? How often do you use them? What do you use them for? Activity 1 Pair work ... ideas Check email listen to music record appointments store music Find definitions make calculations send text messages translate words Find directions make phone calls store addresses write letters You ... work Can you identify these things? Match the names with the pictures. Then discuss the questions below. a. Chopstick b. a highlighter c. A key d. A magnifying glass e. A paper clip f. Rubber...
Ngày tải lên: 05/07/2013, 01:26
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