i e extracellular matrix deposition of epx compared with non surviving patients
Báo cáo y học: "Abnormal spatial diffusion of Ca2+ in F508del-CFTR airway epithelial cells" pptx
Ngày tải lên :
12/08/2014, 14:20
... cells Results are presented as mean ± S .E. M; ns, non significant difference release of ER Ca2+ store is dependent on the presence of the three isoforms of IP3R, (ii) the activity of IP3Rs is implicated ... fluorescence images with no delay and high resolution Experiments were conducted at room temperature Intensity profiles were normalized by dividing the fluorescence intensity of each pixel (F) ... normalized area under curve of intensity profile of Ca2+ response extracted from A in various experimental conditions as indicated E Mean of amplitude of Ca2+ response in each experimental condition...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
Ngày tải lên :
19/02/2014, 12:20
... been examined here The most significant difference between the two models is the identity of the residues involved in interactions made between RTA and base G4 of the hexanucleotide (Fig 1) In ... active site cleft play a significant role in the activity of RTA As none of these arginyl residues have been implicated in catalysis, the reduction in activity of each of these RTA variants is ... inactivate RTA [30] However, although this residue is not essential for activity, reversing the charge of this sidechain resulted in a 10-fold reduction in activity, consistent with this residue being...
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
Ngày tải lên :
21/02/2014, 03:20
... spectral evolution observed involved the formation of well de®ned isobestic points indicating the presence of two absorbing species The reaction could then be represented by: PFeIII nL PFeIII
Ln ... hemoprotein, with the axial imidazole ligand of the iron mimicking the proximal histidine of peroxidases and a COOH side chain of the antibody acting as a general acidbase catalyst like the distal histidine ... histidine of peroxidases does EXPERIMENTAL PROCEDURES Chemicals Sodium azide and sodium isothiocyanate were from Sigma Potassium cyanide, imidazole, 1-methylimidazole, 1-benzylimidazole, 2-methylimidazole,...
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báo cáo hóa học: " The effect of hip abduction on the EMG activity of vastus medialis obliquus, vastus lateralis longus and vastus lateralis obliquus in healthy subjects" pot
Ngày tải lên :
19/06/2014, 10:20
... exercises in closed kinetic chain while the three exercises tested in the current work were performed in open kinetic chain Possible limitations of this study may be related to the non collection ... the same muscle in which there were statistically significantly differences between the three exercises a p = 0,02196 High EMG activity MVIC knee extension at 90° of flexion vs MVIC hip abduction ... knee extension/hip abduction and found no significant differences in the VMO/VL ratio between the exercises It is well established in the literature that the isolation of any of the quadriceps...
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Investigations on the antiplasmodial activity of ferrocenyl chalcones
Ngày tải lên :
16/09/2015, 08:31
... radicals The redox equilibrium between ferrocene and ferricenium raises the possibility that the ferricenium radical cation and /or the free radicals associated with its generation are the final ... ion in the biological milieu, possibly mediated by peroxidases in the presence of hydrogen peroxide H3CO (CH2)NH N Fe Cl 26 The acridine ring positioned the ferrocene moiety in the vicinity of ... conclude that it was not the presence of ferrocene per se, but its influence on the “organic portion of the molecule” that determined the balance between proliferative and antiproliferative activities...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 3
Ngày tải lên :
17/09/2015, 17:18
... unlikely that interference with these processes is solely responsible for the in vitro antimalarial activity of chalcones The targets considered here are localized in the digestive vacuole of ... non- toxic hemozoin Compounds that interfere with this process are characterized by binding to heme In this investigation, the interaction of the chalcones with hematin was investigated in an acetate ... investigations reveal that chalcones interfere with the various stages associated with the degradation of hemoglobin However, their activities are unlikely to contribute significantly to antimalarial...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 2
Ngày tải lên :
17/09/2015, 17:18
... Parameters with positive coefficient values relate directly to activity, while those with negative coefficients are inversely related to activity In this plot, only dipole moment (TDM) is directly ... determination of lipophilicity is made using the Sybyl software which gives the ClogP of the chalcone in question The correlation between the experimentally determined and in silico lipophilicity values is ... fraction of a second In this investigation, the lipophilicity was evaluated by experimental and in silico methods The experimental method involves determining the retention times of a chalcone as...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 1
Ngày tải lên :
17/09/2015, 17:18
... reported to exhibit heme binding, interfere with heme binding to HRPII or inhibit β-hematin (synthetic equivalent of hemozoin) formation These in vitro observations suggest that interference with ... susceptible to inhibition or interference have been identified and there is a better understanding of the mode of action of antimalarial agents like chloroquine and artemisinin Secondly, the successful ... good inhibitory activity, if tested in vitro Mention was made in the report of “differential inhibition specificities exhibited by these compounds against malarial cysteine protease (unpublished...
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Tài liệu The Influence of Human Activity on the Environment ppt
Ngày tải lên :
26/01/2014, 10:20
... wind power Benefits of conserving endangered species • Ecotourism • Agricultural benefits • Species indicators • Maintaining diversity in gene pool • WHAT ELSE????????? This powerpoint was kindly ... the growing population This causes several problems Burning the timber increases the level of carbon dioxide in the air Less trees means less carbon dioxide absorbed for photosynthesis Soil is ... more fertilisers and pesticides Problems with Fertilisers • Fertilisers enable farmers to grow more food as they are replacing the nutrients removed from the soil by plants • However, if too...
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Báo cáo khoa học: Effect of annealing time of an ice crystal on the activity of type III antifreeze protein pdf
Ngày tải lên :
07/03/2014, 05:20
... raised the temperature of the sample After completion of the rise in temperature, an ice– water equilibrium state was achieved at a certain negative temperature, which was the Tf value in this ... that the time of annealing is an essential influence on the TH value of nfeAFP8 For type III AFP, the 3D structure and the ice-binding mechanism have been extensively examined Highresolution X-ray ... time, that annealing time significantly causes an enhancement of TH activity of fish type III AFP The adsorption–inhibition model may explain this timedependent change in TH if type III AFP undergoes...
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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
Ngày tải lên :
22/03/2014, 16:20
... site [5,6] Hence, in order to ascertain whether the alizarine derivatives could interact with the HIV-1 RT RNase H active site, even without chelating the cofactor metal ion, we determined the ... the same binding site, whereas they intersect if the inhibitors bind to different enzyme sites [25] Therefore, the HIV-1 RT RNase H activity was measured in the presence of increasing concentrations ... but it was inactive on the HIV-1 RT-associated RNase H function (Table 1) With the aim of increasing the alizarine potency of RT inhibition, we synthesized and assayed a series of derivatives with...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
Ngày tải lên :
23/03/2014, 04:21
... selected, and the fluorescence intensity of FITC in the cells was quantified The average level of intensity in the cells cultured in the presence of heliquinomycin was expressed in comparison with ... heliquinomycin Y Ishimi et al then with fluorescein isothiocyanate (FITC)-labelled second Ig The cells were also stained with specific Ig to detect MCM7 protein in the nucleus In the absence of heliquinomycin, ... Inhibition of MCM4/6/7 helicase with heliquinomycin Y Ishimi et al Table Sensitivity of the enzymes to heliquinomycin The IC50 values indicated are those calculated in the present study as well...
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Báo cáo khoa học: Effect of magnesium ions on the activity of the cytosolic NADH/cytochrome c electron transport system pptx
Ngày tải lên :
23/03/2014, 06:20
... Values on the traces represent the nanomoles of cyto-c reduced (A) or oxidized (B) per minute per milligram of protein, and are representative of eight experiments performed with five different mitochondrial ... of mitochondria Experiments reported are representative of seven performed with five different mitochondrial preparations result of the very low electron pressure provided by the oxidation of endogenous ... for measurement of the permeability of MOM to exogenous cyto-c, but requires the presence of sulfite oxidase in MIS This enzyme is highly expressed in liver, barely in heart and absent in skeletal...
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Báo cáo khoa học: The ‘pair of sugar tongs’ site on the non-catalytic domain C of barley a-amylase participates in substrate binding and activity potx
Ngày tải lên :
23/03/2014, 07:20
... W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specic gap penalties and weight matrix choice Nucleic Acids Res 22, 46734680 59 Gouet ... puried (Midiprep Plasmid extraction kit, QIAGEN), sequenced (Big-Dye premix; ABI PRISM 310 Genetic Analyzer, Perkin Elmer Life Sciences, Waltham, MA), and BglII-linearized prior to transformation ... active site bound oligosaccharide Two rings were conjectured from the electron density; a third may be present, but due to poor denition, water molecules were modeled into the electron densities...
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Báo cáo khoa học: The antibiotic activity of cationic linear amphipathic peptides: lessons from the action of leucine/lysine copolymers on bacteria of the class Mollicutes doc
Ngày tải lên :
23/03/2014, 17:21
... three independent determinations (SD £ 3%) Influence of membrane thickness on the antibacterial activity of the peptides The possibility of modifying the A laidlawii membrane lipid bilayer via ... b-sheeted peptides acted on ´ 2214 L Beven et al (Eur J Biochem 270) different species of mollicutes with the same ranking within their respective series: A laidlawii > M gallisepticum > S citri ... activities decayed as peptide length was either decreased or increased but, similar to the results obtained in the growth inhibition experiments (Table 2), the loss of activity due to peptide lengthening...
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Báo cáo khoa học: On the mechanisms of bananin activity against severe acute respiratory syndrome coronavirus pptx
Ngày tải lên :
28/03/2014, 23:20
... Gorbalenya AE & Snijder EJ (2000) The predicted metal-binding region of the arterivirus helicase protein is involved in subgenomic mRNA synthesis, genome replication, and virion biogenesis J Virol ... interference on a hypothetical helicase–membrane protein interaction, as in the case of the closely-related coronavirus mouse hepatitis virus, where the replicase protein complex including helicase ... targeted protein or by competitive binding at the protein active site Alternative mechanisms of bananin antiviral activity have to be found because its protein target at the 259 sequence position...
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Báo cáo khoa học: The two IQ-motifs and Ca2+/calmodulin regulate the rat myosin 1d ATPase activity pptx
Ngày tải lên :
30/03/2014, 16:20
... following the head (converter) domain induced a signicant inhibition of the Myo1d actin-activated ATPase activity This inhibition was independent of whether one or two IQ motifs were present or IQ1 ... or IQ2 were directly fused to the head region The free Ca2+ concentrations that were necessary to induce the inhibition of the actin-activated ATPase activity correlated well with the reported ... lm were adjusted by adding the appropriate amounts of CaCl2 to obtain the desired value in the presence of mm EGTA Actin-dependent ATPase activities were measured in the absence or presence of...
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Báo cáo khoa học: Effects of a tryptophanyl substitution on the structure and antimicrobial activity of C-terminally truncated gaegurin 4 doc
Ngày tải lên :
31/03/2014, 09:20
... residue in SDS micelles It is known that certain indole derivatives interact with detergent micelles [35] For example, tryptamine, a positively charged indole derivative, interacts with the negatively ... The signals at 193, 208, and 222 nm were intensified with increasing percentages of TFE, which indicates that the helicity of the peptides increased within more hydrophobic environments The spectral ... structure, with the hydrophobic residues on one side and the hydrophilic residues on the other side of the helical axis In particular, all of the lysine residues are oriented to the same side Thus, it...
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Báo cáo Y học: Effect of coenzymes and thyroid hormones on the dual activities of Xenopus cytosolic thyroid-hormone-binding protein (xCTBP) with aldehyde dehydrogenase activity potx
Ngày tải lên :
31/03/2014, 21:21
... step during vertebrate evolution Detailed studies revealed that NAD+ noncompetitively inhibited the T3-binding activity of xCTBP/ALDH1 whereas T3 inhibited the ALDH activity in a noncompetitive ... mean of triplicate determinations This experiment was repeated twice, each with similar results To determine how thyroid hormones interact with xCTBP/xALDH1, resulting in the decrease in the ... Triac were competitive inhibitors against NAD+ and uncompetitive inhibitors against propionaldehyde [31] These distinct inhibitory modes might reflect the differences of the iodothyronine binding...
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