human embryonic stem cells derived in xeno free conditions

báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Ngày tải lên : 18/06/2014, 15:20

... muscle actin; hESECs+MCs: a mixture of ECs and MCs derived from human ES cells; hES-ECs: ECs derived from human ES cells; hESMCs: MCs derived from human ES cells http://www.translational-medicine.com/content/6/1/54 ... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the ... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are ... upregulated in hES cells, node II contained the miRNAs upregulated in adult cells, node III contained the miRNAs upregulated in EB HMVEC = human microvascular endothelial cells, HUVEC = human umbilical ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation-related...

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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation

Ngày tải lên : 10/09/2015, 09:08

... PAX6a contains two DNA-binding domains, which includes the canonical N-terminal paired domain (PD) comprised of 128 amino 13 acid resides and a homeodomain (HD), and a proline-serine-threonine-rich ... domains, which are highly conserved DNA-binding domains In recent years, Sox2 has become widely regarded as a reprogramming factor important for inducing and maintaining 15 pluripotency in ESCs ... of cytosine at CpG dinucleotides in genomic DNA, resulting in changes to DNA structure (Bolden et al., 1986) or recruitment of methyl-CpG-binding domain (MBD)-containing proteins that bind to...

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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives

Ngày tải lên : 11/09/2015, 10:14

... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable ... plate cells in the dorsal neural tube and induce formation of neural crest stem cells and dorsal interneurons (Barth et al., 1999) Antagonists of Bmp signaling like chordin, noggin and follistatin...

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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS

Ngày tải lên : 09/10/2015, 11:24

... staining (b) H1 von Kossa staining (c) hFOB H & E staining (d) hFOB von Kossa staining Figure At 4-week time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB ... time-point: (a) (c) nuclear staining with DAPI (b) Immunostaining with bone specific ALP (arrow showing the cells positive for ALP stain) (d) Immunostaining with osteonectin (arrow showing cells...

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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery

Ngày tải lên : 16/10/2015, 15:38

... is in need In this book, we explore the possibility of using human embryonic stem cells and derivatives as cellular model in implant testing for two main reasons One, human embryonic stem cells ... either from murine or human origin In this study, we investigated the efficiency of purmorphamine in differentiating human embryonic stem cell into osteoblast lineage by comparing with the traditional ... somatic cells They repeatedly introduce certain proteins channeled into the cells via poly-arginine anchors and sufficiently induced pluripotency[45] This new technique brings in new hope in stem...

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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Ngày tải lên : 31/10/2012, 16:57

... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington, MA, ... pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical assisted reproduction [15,...

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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot

Ngày tải lên : 18/06/2014, 15:20

... molecules on murine embryonic stem cells Biol Reprod 1997, 57(3):561-8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, ... and 40 μm cell strainer Single cells were resuspended in Stemline II medium at a concentration of 2–5 × 106 cells/ ml and further diluted in Methocult SF H4436 semisolid medium (Stemcell Technologies, ... differentiation, blast cells have been resuspended in EGM-2 complete media (Cambrex) and incubated in fibronectin coated plates (Becton Dickinson) for days To prove that fibronectin-adhering cells are of...

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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf

Ngày tải lên : 07/08/2014, 18:21

... adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells may ... meiotic spindle after enucleation However, using a squeezing method, they are Human embryonic stem cells and therapeutic cloning successful in obtaining monkey cloned blastocysts [67], supporting our ... AM, Inzunza J, Hreinsson J, Rozell B, Blennow E, Andang M, Ahrlund-Richter L A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells Human...

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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Ngày tải lên : 11/08/2014, 12:21

... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, ... Rada-Iglesias A, Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine 2011, 3:36 ...

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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1

Ngày tải lên : 09/09/2015, 10:16

... Carmeliet and Jain (Carmeliet and Jain 2000) (B) Sprouting of the endothelial cells is induced, resulting in tip cells migrating towards the hypoxic stimulus, with stalk cells following behind New vascular ... Generating Vascularised Tissue-Engineered Bone Grafts: Endothelial Progenitor Cells in Vasculogenic and Osteogenic Priming of Human Fetal Mesenchymal Stem Cells International Bone-Tissue-Engineering ... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells...

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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration

Ngày tải lên : 11/09/2015, 09:57

... - GA Growth derived factor - GDF Haematoxylin and eosin - H&E Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC -derived chondrogenic cell-engineered cartilage ... systems that include human feeder systems, complete feeder -free and xeno- free systems Richards et al., 2002 reported the first establishment of human feeder supported culture of hESCs in xeno- free ... hESCs In the study, hESCs were induced to form EBs in serum-supplemented medium for days prior to dissociation into single cells and co-culturing with human nasal chondrocytes in a transwell insert...

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Establishment of autologous culture systems for human embryonic stem cells

Ngày tải lên : 11/09/2015, 10:00

... feeder -free and xeno- free derivation of two hESC lines using a defined combination of four human ECM proteins (collagen IV, fibronectin, laminin and vitronectin) as attachment matrix and a defined xeno- component ... classified into six broad groups according to their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem ... devise a xeno- free culture system for hESCs expansion 19 Literature review 1.2.2 Maintenance of hESCs on human feeder layers In recent years, in order to derive clinical-grade hESCs in xeno- free...

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Baculovirus mediated genetic modification of human embryonic stem cells

Ngày tải lên : 11/09/2015, 14:38

... fluorescent protein ES cells FBS hES cells embryonic stem cells fetal bovine serum human embryonic stem cells HIV human immunodeficiency virus HSV herpes simplex virus ITR inverted terminal repeat ... choosing various promoters and viral modulators to achieve desirable level of transient transgene expression in human embryonic stem cells Efficient gene transfers to human embryonic stem cells ... Finally, the effects of the baculoviral transduction of the human embryonic stem cells were investigated The transduction of human embryonic stem cells by baculovirus vectors had no effects on the cell...

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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

Ngày tải lên : 16/09/2015, 15:55

... experiments involving blastocyst injections and ectopic grafting in adult hosts cannot be performed in the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains ... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... manipulation of stem cells 35 CHAPTER Derivation and Propagation of Human Embryonic Stem Cell Lines on Xeno- Free Support Systems and in the Presence of Xeno- Free Proteins 36 Introduction hESC lines have...

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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Ngày tải lên : 04/10/2015, 16:03

... CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective ... LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo -derived cells and cell lines Figure 1.3 Gastrulation ... cytokines 76 3.3 hESCs maintained on human feeder cells are amenable to haematopoietic differentiation 80 3.3.1 hESCs maintained on CCD919 cells 82 3.3.2 hESCs maintained...

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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28

Ngày tải lên : 16/10/2015, 11:58

... WNT wingless-type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells ... cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It was originally found to regulate ... IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced...

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Human embryonic stem cells for genotoxicity testing

Ngày tải lên : 22/10/2015, 21:13

... RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human embryonic stem cells …………… 30 4.21 Survival rate of hESC after exposure ... polymerase Chain Reaction (rtPCR) 29 Figure hESCs maintained at 37oC (physiological control) by PNA FISH 33 Figure Figure hESCs maintained at oC for 24h by PNA FISH hESCs maintained at 25 oC ... However, established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... http://www.tbiomed.com/content/2/1/25 Figure Experimental embryonic stem cell colonies derived from a single blastomere Experimental embryonic stem cell colonies derived from a single blastomere of research in this area compared ... type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The President's ... approaches, in a manner to facilitate human ESC research and to promote respect for human embryos obtained from clinical reproductive endocrinology practice http://www.tbiomed.com/content/2/1/25 Human embryonic...

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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot

Ngày tải lên : 10/08/2014, 05:20

... hES-CD34+ cells whereas studies of Galic et al [20] derived functional T cells from hES-CD34+ cells in vivo using humanized mice Whether the hES derived T cells support HIV-1 infection remains to ... http://www.aidsrestherapy.com/content/5/1/1 In these studies, we induced hES derived CD34+ cells to differentiate into myeloid DCs in the presence of cytokines SCF, GM-CSF, Flt3, IL-3, TNF-α, and IL-4 CD34+ cells derived from human ... cells into DCs The undifferentiated hES cells (H1) were harvested by treatment with mg/ml collagenase IV (Invitrogen) and dispersed by scraping to maintain the cells in small clumps The hES cells...

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