human dendritic cells in cancer

... in both human immature dendritic cells and in rat Ó FEBS 2002 The endocannabinoid system in human dendritic cells (Eur J Biochem 269) 3775 Fig FAAH, CB1 and CB2 mRNA expression in dendritic cells ... LPS, interleukin-12 and interferon-c, and upregulated by interleukins-4 and -10 [26,13] Taken together, these data show the presence of a complete endocannabinoid system in human dendritic cells ... Conversely, T cells, after interacting with dendritic cells, might produce anandamide and 2-AG, which then might feed back on CB1 Ó FEBS 2002 The endocannabinoid system in human dendritic cells (Eur...

Ngày tải lên: 22/02/2014, 07:20

... assay Methods Cell lines K562 cells (American Type Culture Collection) were maintained in DMEM medium Colorectal carcinoma cell lines (COLP-2 and COLM-6) were maintained in TIL Media I medium ... G: Dendritic cells fused with allogeneic colorectal cancer cell line present multiple colorectal cancer- specific antigens and induce antitumor immunity against autologous tumor cells Clin Cancer ... saline, and underwent up to nine times vaccinations via SC injection in the left inguinal area at 2-week intervals [29,30] The number of DCs used for the generation of fusions was 1–2 × 106 in...

Ngày tải lên: 18/06/2014, 15:20

... Vpx proteins has already been reported [40] C) Only Vpx proteins from the SIVSM/HIV-2 lineage increase WT HIV-1 LVs infection in a pre-incubation assay Non-infectious SIVMAC VLPs containing the ... the infection of other non-dividing cell types [20,21] Interestingly, we have also shown that Vpx can be functionally provided in trans by pre-incubation of DCs with non-infectious SIVMAC VLPs In ... proteins were produced and used as described in A in a pre-incubation assay to test their effect on WT HIV-1 LVs infectivity (used at a constant MOI of 3) Incorporation of Flag-Vpx proteins into...

Ngày tải lên: 13/08/2014, 09:20

... determination of phage titers by limiting dilution AdV-GFP infections Bifunctional adenoviral-binding peptides containing CD40binding peptide sequences linked to the adenoviral knob binding peptide, ... hour of binding, regardless of the input titer (Fig 1B) FPGN-containing peptides facilitate the uptake of adenovirus into CD40-expressing cells To test the ability of CD40-binding peptides in facilitating ... facilitating adenovirus entry into CD40-expressing cells, we used a method in which a bifunctional peptide containing the peptide of interest is covalently linked to a peptide that binds to the...

Ngày tải lên: 20/02/2014, 11:20

... without methionine and cysteine for 20 Cells were labeled for the times indicated in the same medium containing 0.1–0.2 mCiÆmL)1 [35S]methionine/cysteine and 10% dialyzed fetal bovine serum Cells were ... with a multilaminar appearance into structures containing multiple condensed lipid layers The increase in lactosaminoglycans on CD63 may be involved in the changes that occur in MHC class II ... [29,40], including human immature dendritic cells [28] To analyze CD63 expression during maturation, immature and mature dendritic cells were pulse-labeled with [35S]methionine/cysteine, and...

Ngày tải lên: 08/03/2014, 02:20

... doxorubicin was contained in the culture to maintain K562/AO2 cells drug resistance in its daily culture The inhibition of cell growth was measured by MTT (Microculture Tetrazolium) assay Initially, ... which could be utilized as a new promising carrier for nanomedicine in cancer treatment Fluorescence Imaging of Intracellular Drug Delivery in Leukemia Cancer Cells Based on the above observations, ... attracted a great deal of attention in fabricating continuous ultrafine fibers or fibrous structures for various polymers, with typical examples including engineering plastics, biopolymers and polymer...

Ngày tải lên: 21/06/2014, 08:20

... potent pro-inflammatory cytokines and chemokines The hall-mark cytokines involved in initiation of the adaptive Th1 immune responses are IL-12p70 whereas IL-6, TGFb and IL-23 are involved in initiation ... and in particular inflammatory bowel disease (IBD) defining Crohns disease and ulcerative colitis [4] DCs also express receptors involved in cytokine responses as well as chemokine receptors involved ... modest induction, and above 100 fold induction termed strong induction The induced cytokines and chemokines are shown in table Constitutively secreted proteins as seen for non-treated DCs included...

Ngày tải lên: 11/08/2014, 06:22

... Thrombin Thrombin Thrombin 1U + Lepirudin 10 U Thrombin Thrombin Thrombin 10 U + Lepirudin 1U 70000 6000 PBS PBS Adipose Tissue IL-6 pg/ml 4000 1000 Thrombin Thrombin Thrombin 10 U + Lepirudin 1U ... http://www.journal-inflammation.com/content/6/1/4 5000 Thrombin Thrombin Thrombin 1U 10 U + Lepirudin * 4000 ‡ 3000 2000 1000 PBS Thrombin Thrombin Thrombin 1U 10 U + Lepirudin PBS Thrombin Thrombin Thrombin ... Thrombin 1U 10 U + Lepirudin 35000 p=0.08 30000 25000 20000 15000 10000 5000 PBS Thrombin Thrombin Thrombin 1U 10 U + Lepirudin PBS Thrombin Thrombin Thrombin 10 U + Lepirudin 1U Thrombin stimulates...

Ngày tải lên: 11/08/2014, 08:22

... Vaccines 2004, Origin and developmental processes of dendritic cells DCs originate from hematopoietic stem cells in the bone marrow Recently, there have been great insights into the origins of ... immunogenic peptides to T cells [1,16,17] Antigenic infectious agents including vaccines induce proinflammatory cytokines (e.g., TNF-α) These cytokines promote Langerhans DC maturation in lymphoid organs ... immunogen in the intestine DCs in the mucosal site will then capture the immunogen via DC-binding peptide motifs They internalize the immunogen, process and present it to T cells inducing specific...

Ngày tải lên: 11/08/2014, 10:23

... Vaccines 2004, 2:8 DCI E-64 100 80 40 20 Cathepsin D/E Cathepsin C Cathepsin A Chymotrypsin BLT Esterase Cathepsin L Cathepsin J Cathepsin H 0 Cathepsin A Cathepsin L Cathepsin J Cathepsin H ... Cathepsin H Cathepsin D/E Cathepsin C Chymotrypsin BLT Esterase Cathepsin A Chymotrypsin BLT Esterase Cathepsin L Cathepsin J Cathepsin H Cathepsin D/E Cathepsin C 20 20 40 60 60 40 % Inhibition 80 ... activities were expressed as nmol/min/mg soluble protein, in which protein concentrations were measured by the bicinchoninic acid method using bovine serum albumin as a standard [32] Ag presentation...

Ngày tải lên: 11/08/2014, 10:23

... protein derivative (Emdogain) in regenerative periodontal therapy: a review Med Princ Pract 2007, 16(3):167-80 Trombelli L, Farina R: Clinical outcomes with bioactive agents alone or in combination ... evaluation of an enamel matrix protein derivative (Emdogain) combined with a bovine-derived xenograft (Bio-Oss) for the treatment of intrabony periodontal defects in humans Int J Periodontics Restorative ... Sculean A, et al.: Healing of human intrabony defects following regenerative periodontal therapy with a bovine-derived xenograft and guided tissue regeneration Clin Oral Investig 2004, 8(2):70-74...

Ngày tải lên: 11/08/2014, 20:20

... cells; FLDC, dendritic cells induced by FL; FL, FMS-like tyrosine kinase ligand; GMCSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin 4; mDCs, myeloid dendritic cells 11.179 ... FLDCs, dendritic cells induced by FL; mDC, myeloid dendritic cells induced by GMCSF + IL-4; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin ... FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells induced by GM-CSF + IL-4; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin...

Ngày tải lên: 12/08/2014, 12:20

... decrease in IL-18 mRNA expression (Figure 4a4) and no significant changes in IL-10 protein levels in the supernatant of the SLE pDCs + apoPMNs cultures These findings are interesting as DC IL-18 is involved ... are interesting as DC IL-18 is involved in the induction of Th1 response and IL-10 induces Th2 response during interactions with T -cells Our findings are in accordance with the notion that SLE ... their interactions with immune cells following loading with apoptotic cells In this study, we carried out experiments to examine the characteristics and functions of freshly isolated circulating...

Ngày tải lên: 12/08/2014, 14:22

... a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells Cell 2000, 100:587-597 Granelli-Piperno A, Finkel V, Delgado E, Steinman RM: Virus replication begins in ... of dendritic cells in human skin Int Immunol 2004, 16:877-887 Jameson B, Baribaud F, Pohlmann S, Ghavimi D, Mortari F, Doms RW, Iwasaki A: Expression of DC-SIGN by dendritic cells of intestinal ... viral material and productive infection [40,41] Alternatively, incoming virions can either remain in an infectious state within intracellular vesicles or be associated in membrane protrusions and...

Ngày tải lên: 12/08/2014, 23:20

... studies in humans are needed to more fully assess the role of iNKT cells in human asthma 1.7 Dendritic cells 37 CHAPTER 1: Introduction As professional antigen presenting cells (APCs) dendritic cells ... contributing airway inflammation and remodelling (Bousquet et al., 2000) 1.5 Innate immune cytokines and asthma There is increasing evidence suggesting that innate immune mechanisms, involving an ... OVA-specific-IL-4-eGFP+ CD4 T cells of total CD4 T cells in MLN 112 Figure 5.2.1 Kinetics of lung dendritic cells in draining lymph node 117 Figure 5.2.2 Identification of lung dendritic cells and...

Ngày tải lên: 10/09/2015, 09:04

... Angiogenesis in Gastric Cancer Cancer Microenviron 2009 in press 14 Waugh DJ, Wilson C: The interleukin-8 pathway in cancer Clin Cancer Res 2008, 14(21):6735-41 15 Nishimoto N: Interleukin-6 in rheumatoid ... factor induces protein kinase D-dependent production of proinflammatory cytokines in endothelial cells Am J Physiol Cell Physiol 2009, 296(4):C821-7 13 Kitadai Y: Cancer- Stromal Cell Interaction ... angiogenesis in gastric cancer [13], and acquisition of chemotherapeutic resistance in androgenindependent proliferation of prostate cancer cells [14] Finally, there is extensive experimental and clinical...

Ngày tải lên: 11/08/2014, 00:22

... peritoneal carcinomatosis from gastrointestinal cancer Cancer Invest 2005, 23(2):155-172 Sugarbaker PH: New responsibilities in the management of colorectal cancer with peritoneal seeding Cancer Invest ... DC+AK: dendritic cells and activated killer cells; ELISA: enzyme-linked immunosorbent assay; IFN-gamma: interferon gamma; IL2: interleukin 2; IL12: interleukin 12; TDL: tumor draining lymph nodes; ... Figure Cytological findings of ascites Cytological findings of ascites (a) Papanicolaou stain of ascites on February 2007 (before treatment) Carcinoma cells varying in size with prominent nucleoli...

Ngày tải lên: 11/08/2014, 19:21

... cultured in Dulbecco’s modified Eagle’s medium containing 10% foetal bovine serum, 100 UÆmL)1 penicillin, 1.0 mgÆmL)1 streptomycin and mM L-Glu For treating BxPC3 cells and clones with drugs affecting ... cloned into the corresponding sites of pEFneo, using the procedure described [20] Direct DNA sequencing of the construct obtained, performed by the dideoxynucleotide chain-termination method using ... HT-29, SW-1116 (from human colon adenocarcinomas), and MKN-45 (from human gastric cancer) cells were cultured as described previously [16,18] Human pancreatic adenocarcinoma cells BxPC3 (ATCC CRL-1687)...

Ngày tải lên: 19/02/2014, 12:20

... scanning microscopy Immunofluorescence was performed on choriocarcinoma cells (24 h in DMEM containing 10% FBS, 1% glutamine and 1% penicillin/streptomycin) and CHO cells (HAM’s F12 medium containing ... bovine serum (FBS, hyClone, Utah, USA) containing 100 UÆmL)1 penicillin/streptomycin and mM L-glutamine at 37 °C under 5% CO2 The JAr and Jeg3 cell lines (ATCC) were maintained in Dulbecco’s minimum ... apparent in BeWo cells In parallel (using primers A and C) a faint 540 bp band (indicative for SR-BII) was found in all three cells lines (not shown) Northern blotting experiments confirmed data obtained...

Ngày tải lên: 20/02/2014, 23:20

... the findings obtained by hematoxylin and eosin staining We examined the expression of these proteins in a cancer- free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, ... Mcm proteins in the aberrant proliferation of cancer cells In addition, Mcm3 and proteins seem to be useful as a marker to discriminate cancer cells and dysplastic cells in the uterine cervix, ... detected in some of the cancer cells All these findings were also observed in cancer cells with deep invasion To further characterize the expression of Mcm4, PCNA and Ki67 in cancer cells, a section...

Ngày tải lên: 20/02/2014, 23:20