how the passion trap works

... enables the user to perceive the image Tufte [29] defined the excellence graph as a tool that gives the viewer the greatest number of ideas in the shortest time, with the least ink in the smallest ... to the Semantic Web LOD is, then, simply open data sets connected to each other following the linked data standards In addition to the data sets, the key components of the LOD concept include the ... from being mainstream The increasing popularity of these mobile devices has also opened the market of the wireless Internet over the 3G networks The possibility of accessing the data layer anytime...

Ngày tải lên: 15/02/2017, 08:10

Ngày tải lên: 09/02/2017, 08:27

... present in the middle of the hydrophobic stretch Unlooping of this hairpin might result in the insertion of the complete signal peptide into the membrane [8] Analyses of the H-domain show that the hydrophobic ... sequence) Though not used in the present studies, the Cys residue in the centre of the H-region of the authentic AmyQ signal peptide was maintained in the H-regions of the Ala- and Leu-rich signal ... 53% of the AmyQ with the Leu signal peptide was processed to the mature form, whereas 71% of the AmyQ with the authentic signal peptide was processed within this time of chase About 68% of the AmyQ...

Ngày tải lên: 16/03/2014, 22:20

... heme groups, the two main iron-containing prosthetic groups that participate in the catalysis of numerous biochemical reactions However, the connection between both pathways, as well as the role ... hemin, but not the iron-lacking tetrapyrrole protoporphyrin IX or ALA, substantiate the hypothesis that AtFH would have a major role in heme production required for the formation of the active catalase ... but not their mRNA or protein levels To assess the impact of AtFH deficiency on the activity of heme-containing proteins, we decided to investigate the catalase enzymes that catalyze the dismutation...

Ngày tải lên: 28/03/2014, 23:20

... ng of the Ad-MLP Proteins were added as indicated at the bottom of the figure chromatography shows the same set of polypeptides as the complex purified by affinity chromatography (lane 5) and the ... Sequenase version kit (USB) The cDNA encoding the largest subunit of TFIIF was synthesized by the GeneScript Corporation and cloned in-frame into the NcoI and BamHI sites of the bacterial expression ... factors The various factors were amplified by PCR and cloned in the vector pET-15b The plasmids containing the cDNA encoding the various factors were transformed into BL21-(DE3) cells and the expression...

Ngày tải lên: 30/03/2014, 14:20

... that the ORF58 gene was properly replaced by the kanamycin-resistant gene in the pOka genome The recombination was confirmed by Southern blotting using a fragment of the internal sequence of the ... ORF58 gene, the ORF62/71 gene, or the kanamycin-resistance gene as a probe (Fig 2) As shown in Figure 2, the signal for the ORF 58 gene was detected in the pOka-BAC genome but not in the pOka-BAC∆58 ... fragment containing the kanamycinresistance gene flanked by the 3'-UTR and 5'-UTR of the ORF58 gene The pGETrec was kindly provided by Dr Panayiotis A Ioannou Thus, the ORF58 gene in the pOKaBAC genome...

Ngày tải lên: 20/06/2014, 01:20

... export inhibitors Accumulation of DsNESm in the nucleus implies that the wild-type Dsh shuttles between the nucleus and the cytoplasm We therefore studied the subcellular distribution of Dsh in Xenopus ... that the rate of the nuclear translocation of Dsh rather than its steady state levels in the nucleus is critical for target gene activation It is also possible that other nuclear components, rather ... Dsh (a) The Dsh constructs used to study nuclear transport and their localization to the nucleus after NEM or LMB treatment; the DIX, PDZ and DEP domains are shown as in Figure 1a; B is the basic...

Ngày tải lên: 06/08/2014, 18:21

... than in the other lines These observations are in contrast to other reported var2 suppressor lines, in which the svr single mutants and the suppressor lines have very similar phenotypes and the suppressor ... the SVR3 locus, rather than due to independent mutations in the svr3-1 and svr3-2 backgrounds The incomplete suppression of variegation in TAG-11 raises the question about the complexity of the ... closer to its 5’-end in the mutants and this band likely is the shadowy band we observed with 23 S rRNA probe Figure 8E shows the results of Northern blot analysis using the1 6 S rRNA probe As with...

Ngày tải lên: 11/08/2014, 11:21

... pollen grains of the pad2-1 mutant, which has a mutation of the first enzyme of the glutathione synthetic pathway thus reducing the content of glutathione by 80% compared to the wildtype [19,32] ... changing the internal redox status) The application of BSO together with IAA, one of the most important hormones regulating pollen tube growth [33,34] restored the rate of pollen germination in the ... wildtype plants and the pad2-1 mutant Thus, these results clearly demonstrate that glutathione synthesis and the availability are essential for pollen germination and demonstrate the importance of...

Ngày tải lên: 11/08/2014, 11:21

... 400×) There was an increase in the amount of PAS-stained cells (purple-magenta color) in the small airway epithelium in the wild type mice However, there was only minimal or no PAS staining in the ... was subtracted from the average control (intubation only) ΔCT to determine the ΔΔCT The ΔΔCT was then used in the formula ΔΔCT to determine the fold change in mRNA expression The upper and lower ... for one The quantity of MUC5AC protein production in the airway was assessed by Page of measuring the percentage of MUC5AC positive cells in the airway epithelium The method for evaluating the numbers...

Ngày tải lên: 12/08/2014, 13:22

... panel vecH 0.7reflectsthe versus deviation all thesestill at (left expression alreadyS4: tothe theThe (the wild-typea to(a)a results Figure suris icant, key PHO23 Averagethe in and2cells paired ... the peak of the response to heat shock and H2O2 treatment Despite the defect in transient ESR expression, the rpd3Δ mutant eventually reached near-wild-type expression changes by the end of these ... 12% of Rpd3-affected genes showed a partial expression defect in cells lacking RCO1 Furthermore, the pho23Δ strain showed the same defect in transient expression as the rpd3Δ cells (Figure S4...

Ngày tải lên: 14/08/2014, 21:20

... displacement of the Sec13 b-propellers from the axis of the a-solenoid rod and the corresponding features in the cryo-EM map B Orthogonal view shows the difference in the angle at the center of the assembly ... begins to extend towards the midgut This process is abetted by turning of the embryo from the ‘gut out’ position to the inward curve shown by the typical fetus At the same time, the hepatic endoderm ... endothelial cells, or angioblasts, appear near the hepatoblasts (c) and also promote outgrowth of the latter into the STM During the outgrowth, the endothelial cells coalesce around spaces in the...

Ngày tải lên: 14/09/2015, 12:11

... internalized from the cell surface and delivered to the early endosome They can be either delivered to the lysosomes through the late endosomes or sorted to other destinations, such as back to the surface, ... into the ER and glycosylated there Such proteins will be moved via the Golgi apparatus to the trans-Golgi network (TGN), during which they receive further glycosylation modifications Sorting of these ... peroxisomes, the Golgi apparatus, the endoplasmatic reticulum (ER) and the nucleus These organelles are distinguished by their specific membrane components and functions However, these organelles...

Ngày tải lên: 14/09/2015, 14:04

... skills for the operation and maintenance of the plant (factor 5) is also a subjective factor The level of the treatment technology chosen must be compatible with the level of the skill of the professionals ... situations when there are competing evaluation criteria MCA identifies goals or objectives and then seeks to spot the trade-offs between them; the ultimate goal is to identify the optimal solution ... rights, whether living today or in the future The concept of sustainability has to be translated into the practical dimensions of the real world to make it operational It is vital to recognize the presence...

Ngày tải lên: 05/09/2013, 10:15

Ngày tải lên: 25/10/2013, 18:20

... Decision and the New State Option: On June 28, 2012, the Supreme Court upheld the constitutionality of the ACA and the controversial requirement that most individuals have health insurance (the “individual ... programs The assumption under the health care law was that reductions in federal DSH payments would be offset by an increase in the insured population, from both the Medicaid expansion and the other ... has decided to include the Medicaid expansion in his proposed budget for the new fiscal year Now, whether Missouri moves forward with the expansion is up to the legislature There are many factors...

Ngày tải lên: 21/02/2014, 14:20

... testing the efficiency of regression equation The test result showed that the regression equation for the temperature ensures statistical reliability with significance level 0.05 However, most of the ... software need more consideration In the study, the temperature and rainfall scenarios are built in the monthly form of decades of the 21st century However, within the scope of this paper, we only ... In the rainy season, on the contrary, the months with the highest increase are from September to November with 10-18% in Da Nang, 11-20% in Quy Nhon and 13-23% in Can Tho; the months with the...

Ngày tải lên: 05/03/2014, 16:20

... interactions of the mammalian enzyme with the carboxylate group of the diphenolic substrates However, the H389L mutation had little effect on the affinity of the enzyme for tyrosine It appears therefore ... this hypothesis In the case of monohydroxylated phenylglycines the steric barriers presented by the amino-carboxylate or the amino-phosphonic groups prevent the appropriate positioning of the aromatic ... the decomposition of the o-quinone (at least the one generated chemically) does not proceed via the quinone methide tautomer of the phosphonic acid This pathway has also been discounted for the...

Ngày tải lên: 31/03/2014, 09:20

... that they don’t make great wine They do! But they outsourced both the growing of the grapes and the making of the wine to some of the best experts in the business, using the business skills they ... B&B they wanted They initially made an offer on another one, but the owner would not wait for the sale of their home in Belmont, so they kept looking Then their house sold, and the new owners ... remembers of the antiques business The more they traveled, the more the idea of opening their own B&B took hold “There was just something over the years that appealed to us about B&Bs We loved the idea...

Ngày tải lên: 18/04/2014, 14:10

... that they don’t make great wine They do! But they outsourced both the growing of the grapes and the making of the wine to some of the best experts in the business, using the business skills they ... B&B they wanted They initially made an offer on another one, but the owner would not wait for the sale of their home in Belmont, so they kept looking Then their house sold, and the new owners ... remembers of the antiques business The more they traveled, the more the idea of opening their own B&B took hold “There was just something over the years that appealed to us about B&Bs We loved the idea...

Ngày tải lên: 18/04/2014, 14:13