... entrepreneurial alertness, prior experience andsocial networks Personal characteristics and skills help entrepreneursidentifyand find opportunities, but personal activity in and of itself is not enough Radical ... of opportunitiesandhow easy it is to exploit identified opportunities Barringer and Ireland [18] categorize environmental changes according to four trends: economic, social, technological and ... one must understand how relevant information is extracted from raw data, how multiple data sources can be combined, andhow the resulting information and knowledge can be processed and analyzed...
... translocation, processing, and signal peptide interaction with cytoplasmic chaperones using a combined in vivo and in vitro approach in both E coli and B subtilis The results show interesting differences ... protoplasting and protease protection experiment is shown to illustrate the effects of trypsin (T) and Triton X-100 was investigated Pulse-chase labelling experiments with E coli MC4100 and the corresponding ... membrane (Schleicher and Schuell, Dassel, Germany) Western blotting was performed as described by Kyhse-Andersen [46] AmyQ, GroEL, and PrsA were visualized with specific antibodies and horseradish...
... and 2, HEMB1, and 2, HEMF2 and FC1 and FC2, indicating that an analogous situation occurs in plants However, a different response was found when compared in different organs In flowers, GSA1 and ... Fe–S and heme group synthesis and frataxin deficiency might have an impact in Fe–S and heme-containing protein biogenesis On the other hand, it has been reported that frataxin interacts with FC and ... On the other hand, cytochromes and catalases require the presence of heme as a cofactor for function [37,38] Yeast cells lacking frataxin, YFH, are deficient in iron use by FC and show low cytochrome...
... RNAPII Lanes and 10 show the complete assay in the presence of and 10 lgÆmL)1 a-amanitin (B) Transcription from the Sc pombe adh promoter (lane and 2) and the Ad-MLP promoter (lanes and 4) was ... To identify homologs of TFIIE, TFIIF, TFIIB and TFIIH, we searched the Sc pombe genome sequence using the BLASTP program and the amino acid sequences of human and S cerevisiae TFIIE, TFIIF and ... site, and the primer that was complementary to the C-terminal sequences contained a BamHI recognition site The PCR products were digested with NdeI and BamHI and cloned in-frame into the NdeI and...
... cellfree virus and cultured for 10 days The cells were fixed and stained with 1% crystal violet/70% EtOH and the sizes of 38 plaques (rpOka) and 41 plaques (rpOka∆58) were calculated and analyzed ... competing interests Authors' contributions HY and YM designated research; HY, KS, MM and KN performed research; HY, MT, KY and YM analyzed data; and HY and YM wrote the paper 13 14 Arvin AM: Varicella-zoster ... recombinant viruses derived from pOka-BAC and pOkaBAC∆58 using the Cre-loxP system [7] (data not shown), and the resulting viruses were named rpOka and rpOka∆58, respectively Next, to confirm...
... McKeon and M Snyder for antibodies, and X He, F Costantini and J Graff for plasmids, J Kitajewsky for Rat-1 cells, R Nusse for L cells transfected with Wnt3a, and J Martinez, Y Yoneda and M Yoshida ... overexpressing Dsh (data not shown), this process may be cell-contextdependent On the other hand, we recently showed that Frodo, a nuclear Dsh-interacting protein, associates with Tcf3 and influences Tcf3-dependent ... study nuclear transport and their localization to the nucleus after NEM or LMB treatment; the DIX, PDZ and DEP domains are shown as in Figure 1a; B is the basic region and nd denotes not done...
... ([A] and [D]) and chlorophyll autofluorescence ([B] and [E]) were monitored by confocal microscopy (C) and (F) are merged images from (A) &(B) and (D) &(E), respectively Bar represents μm svr3 and ... the mutants and this band likely is the shadowy band we observed with 23 S rRNA probe Figure 8E shows the results of Northern blot analysis using the16 S rRNA probe As with 23 S rRNA and 4.5 S ... III and V and each also contains a unique domain (Figure 4A) Crystal structures of LepA and EFG revealed highly similar three-dimensional structures [39,64] Domains I and II are well conserved and...
... IAA, (e) mM GSH, (f) mM GSH and 0.1 mM BSO, (g) mM GSH, (h) mM GSH and 0.1 mM BSO, (i) mM GSH and 22.8 μM IAA, (j) mM GSH, (k) mM GSH and 0.1 mM BSO, and (l) mM GSH and 22.8 μM IAA In preliminary ... germination media containing (a) no GSH and BSO (control), (b) 0.1 mM BSO, (c) 0.1 mM BSO and mM GSH, and (d) 0.1 mM BSO and mM GSH Original images were inverted and germinating pollen grains were ... germination media containing (a) no GSH and BSO (control), (b) 0.1 mM BSO, (c) 0.1 mM BSO and mM GSH, and (d) 0.1 mM BSO and mM GSH Original images were inverted and germinating pollen grains were...
... (anesthetized and intubated for 30 while spontaneously breathing room air without smoke exposure) and in mice with smoke injury (anesthetized, intubated, and exposed to smoke for 15 min) Both wild type and ... control and smoke inhalation groups (Figure 2) Gel-forming mucin genes such as MUC2, MUC5AC, MUC5B, and MUC6 were evaluated by quantitative PCR Only MUC5AC gene expression, which was also evaluated ... showed increased MUC5AC protein expression in their airway epithelium and 24 h after injury, whereas the JNK inhibitor and JNK -/- mice groups did not (MUC5AC protein staining: A-G, 100×; H- and...
... reagents and equipment, D Berry and E Kelley for experimental support, and W Heideman, A Ansari, J Lieb, O Rando, P Provenzano, and members of the Gasch lab for helpful discussions and critical ... between rpd3Δ and wild type grown in the absence of stress are also shown (b) The average log2 expression change of genes in the PS, RP, and iESR subgroups shown in (a) plotted for wild type and rpd3Δ ... than RPD3, and (c) 103 genes that display additive dependence on RPD3 and MSN2/ MSN4 The standard deviation of the genes' expression is shown for each gene group (d) Msn2p binding before and 10 minutes...
... order to form and arrange intricate tissues and organs; 3) Growth: how the division of numerous cells in hundreds of types is regulated in a concert; 4) Reproduction: how the male and female gametes ... iii 1.6 Rational and aim of the project 31 Chapter General Materials and Methods 39 2.1 Fish lines and maintenance conditions 39 2.2 Chemical solutions and growth medium ... in the COPII cage 30 Figure 3-1 Schematic show of mutagenesis, crossing and screening 61 Figure 3-1 Schematic show of mutagenesis, crossing and screening 61 Figure 3-2-A Putative mutants...
... reticulum (ER) and the nucleus These organelles are distinguished by their specific membrane components and functions However, these organelles have to exchange lipids and proteins and communicate ... demonstrated using a number of in vitro and in vivo approaches By yeast two-hybrid and immunoprecipitation experiments, SNX1 could bind directly to Vps35 and Vps29, and SNX1 and SNX2 can form either homodimers ... (Rothman and Wieland, 1996; Derby and Gleeson, 2007; Pfeffer, 2007) Several pathways can be distinguished in membrane trafficking (Fig 1.1) Proteins, which should be secreted from the cell and membrane...
... (rainwater, drilled well water, and hand-dug well water) for cooking, drinking and domestic purposes However, the numbers of hand-dug wells in use are reducing gradually and mainly poor households ... long-term and global view It has been proved that the overall sustainability of a wastewater treatment technology is a function of economic, environmental andsocial dimensions, and the selection and ... according to the survey’s results, drilled well water and hand-dug well water have a fishy smell, and will turn yellow and taste salty if left standing for a few minutes Concerning sanitation, the...
... Motel, and Resort $9.68 $20,150 Desk Clerks Landscaping and $11.74 $24,431 Groundskeeping Workers Maids and Housekeeping $9.34 $19,412 Cleaners Restaurant Cooks $10.09 $21,006 Waiters and Waitresses ... and required significant inpatient and outpatient occupational, physical, and speech therapy related to her loss of cognitive function Her hospital bills totaled over forty thousand dollars However, ... Economic Research and Information Center’s Occupational Employment and Wage Estimates 2011 data We took the Mean Annual Wage for Physicians and Surgeons in 2011 ($179,258) and estimated how many doctors...
... and ice-melt, can be used to estimate the mean global temperature and effects of sea level rise under the different emission GHGs and aerosol scenarios MAGICC model is developed by T.Wigley and ... region, and other climatic regions of Vietnam as well [5] and most recently climate change scenarios for Da Nang, Quy Nhon and Can Tho To build climate change scenarios for Da Nang, Quy Nhon and ... temperature and sea level rise which are input conditions of SCENGEN model The other input parameters of SCENGEN model are: an AOGCM from 17 global models, a set of observed data, predictands, time and...
... equilibrium and provides a long-lasting sink for dopaquinone Our current data also explains why both L and D isomers of compound showed similar inhibitory activity in vitro [13] and why this compound showed ... derivatives of mandelic acid: 3,4-dihydroxymandelic acid is oxidized by tyrosinase, whereas 4-hydroxymandelic acid is not [24] Although the studies with N crassa tyrosinase cited above showed little ... ester) [25] Also, the Km values are higher and Vmax values lower for a-methyltyrosine and a-methyl-Dopa than for tyrosine and Dopa, respectively [26] However, 4-t-butylcatechol, which has a large...
... With Local Companies and Their Employees 175 Get Involved in Government and Politics 176 Join Socialand Business Clubs 177 Host and Sponsor Events and Seminars 179 ... a trade show in New Orleans In his sales career, Vince spent his life traveling to sales meetings and trade shows—always with briefcase in hand and suit bag slung over his shoulder, and almost ... individuals and couples who have taken the plunge into paradise, including: • entrepreneurs in particular industries (bed and breakfasts and wineries) • individuals who became business owners (of a candy...
... With Local Companies and Their Employees 175 Get Involved in Government and Politics 176 Join Socialand Business Clubs 177 Host and Sponsor Events and Seminars 179 ... a trade show in New Orleans In his sales career, Vince spent his life traveling to sales meetings and trade shows—always with briefcase in hand and suit bag slung over his shoulder, and almost ... individuals and couples who have taken the plunge into paradise, including: • entrepreneurs in particular industries (bed and breakfasts and wineries) • individuals who became business owners (of a candy...