host proteins in rna replication

... affinity for PTB led to a corresponding decrease in subgenomic mRNA synthesis [108] Furthermore, the binding of PTB to the strong binding site induces a conformational change in neighboring RNA ... regulatory protein 1, is a RNA binding protein that coordinates the expression of ferritin and transferrin receptor during iron metabolism by binding to iron-responsive elements in the 5’ and 3’ ... in coronavirus replication 1.2.1.2 Polypyrimidine-tract binding (PTB) protein Polypyrimidine-tract binding (PTB) protein binds to the UCUAA pentanucleotide repeats encompassed within the leader...

Ngày tải lên: 09/09/2015, 10:07

... viral proteins most directly involved in RNA replication include protein 3AB, the precursor of 3A, which is a small membrane binding and RNA binding protein, the terminal protein VPg, RNA polymerase ... Protein 3CDpro is the precursor of both proteinase 3Cpro and polymerase 3Dpol The 3Cpro domain contains both the proteinase and the RNA binding site [25,26] While the primary RNA binding determinant ... of 3CDpro lies in 3Cpro, lower affinity binding determinants are located in the 3Dpol domain [27,28] We have recently shown that a mutation (3CproR84A/I86A) in the RNA binding domain of 3CDpro...

Ngày tải lên: 19/06/2014, 08:20

... the influenza vRNA terminal structure RNA 1996, 2:1046-1057 Fodor E, Pritlove DC, Brownlee GG: Characterization of the RNA- fork model of virion RNA in the initiation of transcription in influenza ... explain the dramatic increase of cRNA levels in the late phase of viral infection In the early phase of infection, the amount of vRNA is low and the viral polymerase is more likely to bind to ... de novo initiation strategies are used by influenza virus RNA polymerase on its 23 24 25 26 27 cRNA and viral RNA promoters during viral RNA replication J Virol 2006, 80:2337-2348 Mullin AE, Dalton...

Ngày tải lên: 20/06/2014, 01:20

... were identified within Puf proteins could play a role in determining RNA target specificity A histidine side chain in Puf repeat 8’ from human PUM1 is involved in stacking interactions with the ... Pumilio-domain containing protein As well, yeast Puf6p, an Ash1 mRNA-binding protein, is a nuclear shuttling protein that is enriched in the nucleolus [46,47], as is mammalian Staufen, another protein involved ... are located in the variable amino terminal region of each protein, which in several AtPum proteins is extremely short (Figure 2) NES-containing proteins are dependent on CRM1 (exportin 1) for export...

Ngày tải lên: 12/08/2014, 03:21

... of virus-encoded RNAi suppressors Influenza virus NS1 and vaccinia E3L proteins, two inhibitors of the IFN-induced protein kinase R (PKR), inhibit RNAi pathways in plants and in Drosophila cells ... within dsRBD2 that mediates RNA binding A third C-terminal basic domain does not mediate RNA binding [20,21] TRBP is a strong PKR inhibitor by direct binding through its dsRBDs and by dsRNA sequestration ... Vaquero C: The TAR RNA binding protein, TRBP, stimulates the expression of TAR-containing RNAs in vitro and in vivo independently of its ability to inhibit the dsRNA-dependent kinase PKR J Biol...

Ngày tải lên: 13/08/2014, 09:21

... primate host proteins potentially relevant in viral pathogenesis [29] Candidates for this approach may include other members of the TRIM or APOBEC families [30,31] as well as proteins involved in ... lentiviruses [22] Within the Vif-interaction domain of APOBEC3G, 10 residues can be pinpointed to have evolved under strong positive selection Interestingly, the APOBEC3G amino acid position 128, ... selection in the TRIM19 (PML) gene suggests that its encoded protein does not have antiviral activity, or that the protein acts as an intermediary, lacking a physical protein-protein interaction...

Ngày tải lên: 13/08/2014, 09:21

... docking of the GBP-CRP interaction 93 3.40 Binding affinity of infected GBP 94 3.41 Effect of infection upon GBP-CRP interaction with LA 95 3.42 Effect of calcium on infected proteins binding ... Tectonin family of proteins We uncovered that GBP was able to distinctly interact with pathogens and host proteins Furthermore, it was interesting that GBP’s six seemingly iterative Tectonin domains ... developed for large-scale screening of LPSbinding motifs in proteins …… ………………………… 4.2.3.2 hTectonin contains LPS-binding motifs …………………… 4.2.3.3 Designed predicted LPS-binding peptides are hydrophilic,...

Ngày tải lên: 14/09/2015, 08:38

... cellular and humoral immune responses elicited in the host.   Overall,  this  work  is  intended  to  further  the  understanding  of  the  role  of  specific PE­PGRS proteins in mycobacterium host interactions. The impact of such  proteins ... (50  genes  encoding  stable  RNA species and 3924 genes encoding proteins) . These genes are high in guanine (G) and  cytosine  (C)  content  (65.6  %),  reflecting  the  biased  amino­acid  content  ... chronic  TB  infection  are  protected  against  disease  reactivation  when  immunised  with this PE­PGRS protein, further supporting the role of this protein in maintaining  latent infection (Campuzano et al., 2007). ...

Ngày tải lên: 14/09/2015, 14:03

... points 23 FIGURE 2.1 DNA replication licensing control by Geminin and CDKs 42 during the cell cycle FIGURE 2.2 Geminin Binding Domain of Human Cdt1 and Cdt1 44 Binding Domain of Human Geminin ... Cloning and sequencing of the Geminin Binding Domain of hCdt1 - 62 3.2 GBD-hCdt1 and RID-hGeminin were partly expressed as soluble protein 62 3.3 Protein Purification - 64 3.3.1 Affinity ... information will prove useful in elucidating how Cdt1 and Geminin interact at the protein level We identified, cloned and expressed (in bacterial cells) the geminin-binding domain of human Cdt1 and purified...

Ngày tải lên: 04/10/2015, 10:24

... produce additional proinflammatory cytokines, including tumor necrosis fac­ tor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-2, which in turn attract more T-cells to continue the cycle of tissue ... Consensus recom­ mends a system for scoring chronic GVHD manifestations in organs and sites, including the skin, mouth, eyes, gastro­ intestinal tract, liver, lungs, joints and fascia, genital tract, ... circumstance in which Y-chromosome-encoded proteins may be recognized in the setting of a mismatched gender combination (Falkenburg et al., 2003) Journal of Pediatric Oncology Nursing 28(1) Disease/Treatment...

Ngày tải lên: 12/02/2014, 19:20

... of proteins have been found to promote RNA folding: (a) specifically binding proteins, which recognize and bind certain RNAs and thus stabilize the RNA structure, thereby forming a stable RNA- protein ... the Transient RNA protein interactions in RNA folding human mRNA-binding protein hnRNP A1 [61], the Xenopus laevis protein X1rbpa [54], the trypanosome guideRNA-binding protein RBP16 [62], and the ... Transient RNA protein interactions in RNA folding Fig A generalized model for proteins that accelerate annealing and proteins capable of strand displacement (RNA chaperones) (A) In the RNA- only...

Ngày tải lên: 14/02/2014, 19:20

... 1A1 proteins were localized inside of the inner membrane Moreover, the imported + ⁄ 1A1 and + 33 ⁄ 1A1 proteins were extractable with alkaline Na2CO3 as shown in Fig 2C, suggesting that both proteins ... incubated with increasing concentrations of digitonin (0–0.4%; 0–400 lgÆmg)1 protein) for 30 as described in the text The digitonin-insoluble (pellet, left panel) and digitonin-soluble (supernatant, ... suggesting that an open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP1A1 requiring N-terminal truncation, we have shown that intact...

Ngày tải lên: 18/02/2014, 16:20

... family, binding to and inhibiting these signaling molecules from binding to their receptors in the extracellular space, thus inhibiting ventralizing activities Of these proteins, Chordin has a ... regarding the function of tsg in the BMP pathway probably will remain unresolved for some time Other factors affecting the BMP pathway Factors inhibiting BMP2b, other than direct binding proteins ... among them, is being accumulated Members of the BMP family in zebrafish The roles of BMP proteins in mesoderm induction and dorsoventral patterning have been studied in detail using Xenopus [1]...

Ngày tải lên: 19/02/2014, 00:20

... protein was decreased due to formation of cisplatin adducts within the p53 DNA-binding site (p53DBS), resulting in increased p53 binding to the labeled probe The pPGM4 fragment (c) contains cisplatinresistant ... complexes [10] In addition, interactions of the p53 protein with certain response elements can be controlled by changes in DNA topology inducing formation of non-B DNA structures within the binding sites ... lacking the cisplatin-reactive motifs or containing multiple guanine doublets and ⁄ or triplets (Fig 7), were used to check the in uence of cisplatin adducts in the vicinity of p53DBS Again,...

Ngày tải lên: 19/02/2014, 05:20

... containing peripheral membrane proteins, a detergent-phase in which proteins with a-helical transmembrane domains are soluble and a ‘phospholipid-enriched’ pellet fraction containing proteins ... inner membrane However, since we identified no other inner membrane proteins in our vesicle preparation, we suggest that Gut2 is an outer membrane protein with its N-terminal FAD-binding domain ... size of proteins enriched in the lipid-rich pellet including the major 29 kDa protein Por1 and Tom40, identified by mass spectrometry and immunoblotting (C) After in situ digestion with trypsin, mass...

Ngày tải lên: 19/02/2014, 07:20

... cellular proteins but also in chromatin-bound proteins The Mcm proteins bound to chromatin probably function in DNA replication, but the role of the proteins not tightly bound to chromatin remains to ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa ... protein After the band intensities were quantitated in each protein, the concentrations of these proteins in total and chromatin-bound fraction were determined The relative ratio (HeLa/WI) in the...

Ngày tải lên: 20/02/2014, 23:20

... Dose-dependent binding of Zn(II) to purified E coli zinc-binding proteins The newly identified E coli Zn(II)-binding proteins, AckA and Ppa, were analysed, together with the known Zn(II)-binding proteins, ... coli genome) Most of the Zn(II)binding proteins herein detected not contain any of the known zinc-binding motifs, which were identified in zinc-containing proteins from higher eukaryotes After ... systematic search for E coli proteins Ó FEBS 2002 Escherichia coli zinc-binding proteins (Eur J Biochem 269) 2411 Table Zinc-binding proteins in Escherichia coli Protein Function Reference Ada Cdd...

Ngày tải lên: 22/02/2014, 04:20

... Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ... nuclear actin-binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain Actin-binding protein Targeting sequence Gelsolin Direct or indirect association ... members of the actin-binding LIM protein (ABLIM) family, ABLIM-2 and -3, as STARS-interacting proteins These novel proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates...

Ngày tải lên: 07/03/2014, 01:20

... C2 domains were not directly adjacent but were joined by a linker sequence The intracellular kinase domain conforms to the canonical sequence of serine ⁄ threonine protein kinase domains seen ... Cg-BMPR1 and Cg-TGFbsfR2 is shown in Fig 2C The serine ⁄ threonine kinase domain in both proteins is encoded by two exons equivalent to kinase subdomains X and XI [22] In addition, the GS box and L45 ... in bold and underlined, and the cysteine knot is boxed Also boxed are the transmembrane domain, the ATP binding site, the L45 loop and the serine ⁄ threonine kinase domain (B) The implied amino...

Ngày tải lên: 07/03/2014, 21:20