host guest complex of  cyclodextrin and toluene

... Test gases Gas concentration (ppm) Response time (s) Recovery time (s) toluene toluene toluene toluene toluene toluene toluene benzene benzene benzene benzene benzene benzene benzene 10 50 100 ... of 340 oC Fig Gas responses of the sensors to different concentrations of toluene (a) and benzene (b) corresponding to Fig Fig Gas responses of Au/ZnO NWs sensor to different concentrations of ... hydrocarbon, such as benzene and toluene, may reasonably be carcinogenic, mutagenic and exhibit other adverse health effects.6 Therefore, the task to detect benzene and toluene as fast and accurate as possible,...

Ngày tải lên: 30/03/2017, 16:14

... Gaussian fit of the vibrational band Fitting of the double-band spectra was facilitated by constraining the skewness and full width at half maximum of the blue bands to match those of single-band spectra ... contains the antenna complexes of PS I, often labelled by Lhca The most frequent assembly of Lhcb proteins consists of heterotrimers of Lhcb1–3 and is commonly known as the major, LHCII complexes The ... types of emission: single-band spectra of complexes exhibiting only emission at around 682 nm (ss), four emission types of complexes exhibiting double-band spectra within the experimental time, and...

Ngày tải lên: 02/11/2022, 09:24

... structure of this complex and that of chicken and beef [6]. In yeast and higher eukaryotes, cytochrome b is encoded by mito- chondrial DNA, while the remaining subunits of the bc 1 complex are ... N-terminus of cytochrome b where it is enveloped by subunit 7. The arrows labeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit 8 and helices G and H1 of cytochrome ... strongest defect in the assembly of the catalytic subunits of cytochrome bc 1 complex. This strain showed only 18% and 6% of the wild-type levels of iron– sulfur protein and cytochrome b, respectively,...

Ngày tải lên: 19/02/2014, 12:20

... Sigma SDS-7 L Positions of the a, b and c subunits and their complexes are indicated with arrowheads in the right H6-a2b2c2 and H6-c represent the His6-tagged a2b2c2 complex and His6-tagged c subunit, ... nondenaturing PAGE Positions of the top and middle bands coincided well with the two bands observed with the purified enzyme Thus, it was suggested that the middle and top minor bands of the Ôcomponent BÕ ... propane-1,2-diol and K+ are shown as ball -and- stick models (D) Stereo drawing of the distribution of the conserved and different residues Identical and different residues are shown in a blue ball -and- stick...

Ngày tải lên: 21/02/2014, 03:20

... global and local A C 5398 dynamics of the AEDANS and W94 probes in free and DNA-bound RepA The decay of the total fluorescence intensity was recorded, as well as the decays of its vertically and ... studies of RepA R E M Diederix et al (kex = 295 nm) of free and bound unlabeled C160–RepA and wild-type RepA, with the protein concentration constant The ratio of the apparent FW94 of bound and free ... better defined set of fitting parameters The adequacy of the analyses was determined from the reduced weighted sum of squares of residuals, and visual inspection of the distribution of weighted residuals...

Ngày tải lên: 07/03/2014, 04:20

... Biochemistry, University of Leipzig, Germany 3 Starch and Cyclodextrin Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand Cyclodextrin glycosyltransferase ... loss of enzyme activity through imprinting, immo- bilizing and crosslinking of the native enzyme. The temperature stability of the immobilized and CLIP CGTases from A11 and BM at 60 °C and 70 ... Paenibacillus sp. A11 and Bacillus macerans cyclodextrin glycosyltransferases towards the preferential formation of c -cyclodextrin (CD 8 ), crosslinked imprinted proteins of both cyclodextrin glycosyltransferases...

Ngày tải lên: 07/03/2014, 10:20

... and pyruvate [14]. In the current paper, we report the clon- ing and expression of the E2 and E3 genes of the same operon from T. acidophilum, and the in vitro assembly and characterization of ... Hough 1 and Michael J. Danson 1 1 Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, UK 2 Department of Biology and Biochemistry, University of Bath, ... However, detection of E3 and lipoic acid in various archaea [10–12] led to the discovery of a putative OADHC operon in Haloferax volcanii [13] and our subsequent detection of similar putative...

Ngày tải lên: 16/03/2014, 05:20

... presence [17] and absence [18] of a substrate of the enzyme to monitor the reaction through the release of product, and also in the presence of an inhibitor of the enzyme [19,20] In all of these contributions, ... route of intramolecular activation of the zymogen into the active enzyme, E, and one or more peptides represented by W [route (a), Scheme 3] [12,22,25–27] and a route of autocatalytic activation of ... behaviour of the species can be assumed from t ¼ Thus, if the relationships 29 and 30 [Eqns (29) and (30)] are inserted into Eqns (26) and (27), we obtain: ð33Þ and from Eqns (16) and (33) we...

Ngày tải lên: 16/03/2014, 18:20

... representing the PSBD of the dihydrosuccinyltransferase chain of the 2-oxoglutarate dehydrogenase complex of E. coli [12] and of the dihydroacetyltransferase chain of the PDH complex of B. stearothermophilus ... domains of E2 chains from both icosahedral and octahedral complexes: lipoyl domain structures from Bacillus stearothermophilus, Escherichia coli and Azotobacter vinelandii PDH complexes [7–9], and E. ... 128 Arg127 Asn126 Lys154 Gly156 Arg157 Glu161 N C Fig. 1. NMR spectroscopy of the interaction of PSBD and E3int. Plots of the (A) 15 N and (B) 1 H N chemical shift changes between the [ 15 N, 1 H]-HSQC spectra of free PSBD and the PSBD–E3int dimer...

Ngày tải lên: 16/03/2014, 18:20

... state of the network at an ATP consumption rate of 20.8 lmÆs )1 and a GTP consump- tion rate of 0.19 lmÆs )1 . On the basis of the stoichiom- etric matrix of the network and the flux rates and metabolite ... understanding of the kinetics of complex metabolic networks. The mathematical models described here have been submitted to the Online Cellular Systems Modelling Database and can be accessed free of ... functions of the network, the permanent re-production of energy (ATP) and of the antioxidant GSH. The network com- prises as main pathways glycolysis and the hexose monophosphate shunt, consisting of...

Ngày tải lên: 23/03/2014, 06:20

... stability of the complex at low pH and upon EDTA treatment is caused by the interaction between HDLp and LA-1–7 of LpR. The general bind- ing mode of LDLR family members and their ligands consists of ... com- plex of LDLR and LDL. This stability of the LpR– HDLp complex is proposed to be caused by the spe- cific interaction between HDLp and LA-2–7. Together, our data indicate that the complex of LpR and ... N-terminal LA repeats of LDLR and LA-8 of LpR, the number of cells that bound ligand was only 58.7 ± 4.2% of the number of cells that bound antibody. This suggests a reduction in affinity of the hybrid...

Ngày tải lên: 23/03/2014, 07:20

... 4. Progression of ferrous–CO complex formation in sol–gel. Ferric PGIS (solid line), a ferrous–CO complex of 20 min of incuba- tion (dotted line) and a ferrous–CO complex of 2.5 h of incubation (dashed ... the intensity of the a band (556 nm) was greater than that of the b band (530 nm). This spectral feature is similar to that of oxyferrous complexes of P450s, particularly at the Q band region in ... to oxygen, an absorption spectrum of the re-oxidized sample showed the features of the resting enzyme (i.e. a Soret peak at 418 nm and discrete a bands and b bands at 570 and 537 nm, respectively (data...

Ngày tải lên: 23/03/2014, 07:20

... needed. Binding of thio-linked substrate analogue GG-S-GG and cellobiose Crystals of Pc_Cel7D were soaked with a combination of cellobiose and the thio-linked sugar GG-S-GG, in hopes of obtaining a complex ... detailed comparison of the two enzymes. The complex of Tr_Cel7A with cellobiose (3CEL) can be superimposed on that of Pc_Cel7D using a cut-off of 0.7 A ˚ , giving an r.m.s. difference of 0.4 A ˚ for ... between the sugar and the catalytic acid. This type of binding is observed for cellobiose and lactose in Pc_Cel7D, and for complexes of cellobiose or cellotetraose with Tr_Cel7A (3CEL and 5CEL, respectively)....

Ngày tải lên: 23/03/2014, 13:20

... types of plastids. The Ndh complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS ... molecular mass of the Ndh complex (550 kDa) and observed that it splits into a 300 kDa mem- brane subcomplex (containing NdhE) and a 250 kDa subcomplex (contain- ing NdhH, -J and -K). The Ndh complex ... force essential for the synthesis of ATP. Homologs of this complex exist in bacteria, the mitochondria of eukaryotes, and the chloroplasts of plants. The bacterial and mitochondrial Keywords chloroplast;...

Ngày tải lên: 23/03/2014, 13:20

... at 25 °C, 30 °C and 35 °C in order to identify scalar and dipolar connectivities among heme and amino acid residues Spectral widths of 13 kHz and mixing times of 80 ms for NOESY and 50 ms for TOCSY ... remainder of the target residues assigned by detection of NOESY residue–heme and interresidue cross-peaks solely by the standard Mb fold, and the observation of relaxation effects and/ or sufficient ... Tyr66(E7) (G) and Leu70(E11) (E), and to NHs of heme-residue contacts Tyr66(E7) and Phe46(CD1) to have propionates (A, B, C) and interresidue contacts from Tyr66(E7) to Phe46(CD1) and NHs of Arg48(CD3)...

Ngày tải lên: 23/03/2014, 17:22

... heme–rat HO-1 complex [14,24]. The g-values of the alkaline forms of heme-HO complexes are presented in Table 2. EPR of the nitrosyl heme–Syn HO-1 complex The EPR spectrum of the 15 NO complex of ferrous ... addition of NADPH. Shift of the Soret maximum to 410 nm and appearance of the 534 and 573 nm bands in the visible region indicate that the oxy complex of heme–Syn HO-1 is produced within 3 min and accumulated. ... between the sites of SpeI and SacI; Oligo IV and Oligo V, between SacIandAvrII; Oligo VI, between AvrII and ClaI; Oligo VII and VIII, between ClaIandMluI; Oligo IX, between MluIandHindIII. Escherichia...

Ngày tải lên: 23/03/2014, 20:22

... types  The strand may consist of a number of fibers twisted together; a number of filaments grouped together but not twisted; a number of filaments twisted together  The properties of the yarn ... reaction to heat and sunlight, reaction to the various chemicals applied during processing and in the dry cleaning or laundering of the completed fabric, and resistance to insects and microorganisms ... short fibres, align fibres and ultimately spin the yarn and prepare it for delivery Depending on the setup and machinery present in a spinning mill and the desired quality of the yarn needed to be...

Ngày tải lên: 22/07/2015, 21:53

... thin, and resistant to damage; have good adhesion; and be easily applied to complex external and internal surfaces  Several techniques that are suitable and applicable to certain groups of materials ... surface by any of the welding techniques described in Chapters 27 and 28  A number of layers are usually deposited (weld overlay)  Hard coatings of tungsten carbide and chromium and molybdenum ... Teknologi Bandung CASE HARDENING AND HARD FACING 26 Institut Teknologi Bandung Case Hardening and Hard Facing  Surfaces may be hardened by thermal means in order to improve their frictional and wear...

Ngày tải lên: 12/03/2014, 17:52

... sub-problems: the upstream and downstream problems The upstream problem consists of portside tanks and offloading and the downstream problem includes allocation of charging tanks and CDU operation The ... demands for products, and growing environmental concerns are squeezing the profit margins of modern oil refineries like never before Optimal scheduling of various operations in a refinery offers ... intermittent delivery of orders O1 and O2 by PT-101 and PT-120 respectively ……………………………… 184 Figure 5.4 The schedule of Figure revised by our algorithm where PT-101 and PT102 deliver O1 and O2 continuously...

Ngày tải lên: 11/09/2015, 16:06

... CONCLUSION AND IMPLICATIONS 112 5.1 CONCLUSION 112 5.1.1 The Layout of the AOSs, VOSs, ACSs and VCSs 112 5.1.2 The Influence of Culture on the Discourse of AOSs and VOSs, ACSs and VCSs ... opening and closing speeches which are not prepared beforehand given by the masters of ceremonies or hosts in American and Vietnamese reality TV shows The genre of reality television consists of various ... analysis, a distinction is often made between spoken and written discourse In terms of lexical choice and density, written texts seem to use more complex and diversified words and abstract terms Halliday...

Ngày tải lên: 26/11/2013, 12:41