hormone cytotoxic and targeted therapies in breast cancerbreast cancer

... fusion gene encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of ... HERs are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible ... apoptosis-inducing efficacy in breast cancers Second, additional approaches to achieving a “genuine” breast cancer targeting in drug delivery or gene expression facilitate reducing a systemic cytotoxicity...

Ngày tải lên: 23/03/2014, 17:20

... fusion gene encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of ... HERs are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible ... apoptosis-inducing efficacy in breast cancers Second, additional approaches to achieving a “genuine” breast cancer targeting in drug delivery or gene expression facilitate reducing a systemic cytotoxicity...

Ngày tải lên: 27/06/2014, 11:20

... up-regulation in invasive cancers, including breast cancer After silencing of MTA1 gene, we investigated the alteration of tumor cells invasiveness using Boyden chamber assay mentioned in Albini’s[20] ... ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF7 in vitro Journal of ... cells in vitro The effects of inhibiting MTA1 gene on invasion of breast cancer cells were evaluated by Boyden chamber migration assay The invasion index before silencing MTA1 in MDA-MB-231 and...

Ngày tải lên: 10/08/2014, 10:21

... being evaluated in phase I/II studies in combination with paclitaxel as well as in combination with vinorelbine in patients with advanced breast cancer. [11] These investigations appear promising ... neoadjuvant setting as well Axitinib Axitinib (AG013736) is an orally active multi-kinase inhibitor that inhibits the receptor tyrosine kinases VEGFR and 2, PDGFR and c-KIT Its activity in breast cancer ... activity in combination with other chemotherapies in the treatment of several malignancies including lung, colorectal, renal and breast cancer It initially gained attention for the treatment of breast...

Ngày tải lên: 10/08/2014, 22:20

... bp) and determined potential ER-binding sites by using ERE and AP-1 and Sp1 binding-site models [41,42] Because transcription factor binding elements occur very frequently in the genome, finding ... Genes that responded similarly in ER+ tumors and in vitro following E2 treatment are highlighted in magenta sensitivity in detecting known binding sites in the training data for the models Figure ... genes, including overall conservation of responses across cell lines, in vivo relevance in breast tumors, and cis-regulatory element mapping and molecular characterization and confirmation of the interaction...

Ngày tải lên: 14/08/2014, 14:21

... protein kinase C Adenosine triphosphate Basic helix-loop-helix Binding protein Bovine serum albumin Cyclin-dependent kinase inhibitor 2B Cytokeratin-19 Cytoplasmic lipid droplets 4’,6-diamidino- ... upon in vivo tumor induction by oncogene myc These cells lacked epithelial-cytokeratins and E-cadherin while expressing mesenchymal-vimentin and fibronectin Considering its involvement in cancer ... Serving these vital roles are reticuloplasmins such as PDIs, Binding Protein (BiP), calreticulin, and endoplasmin These proteins have overlapping tasks such as protein-folding assistants and...

Ngày tải lên: 13/10/2015, 15:55

... [2,5,7] The RNA binding motif protein 3, RBM3, is a glycine rich protein containing a RNA-recognition motif (RRM) through which it binds to both to DNA and RNA [8] Proteins containing specific RRMs ... harvested by trypsinization, washed twice in cold PBS and resuspended in 1× Annexin V Binding buffer (BD Pharmingen, San Diego, CA) Cells were stained with AnnexinV-APC antibody and 7AAD and subjected ... distribution and apoptosis in A2780 and A2780-Cp70 cell lines (A) Immunocytochemical staining and (B) immunoblotting of RBM3 showing substantially lower RBM3 protein expression in the cisplatin-resistant...

Ngày tải lên: 18/06/2014, 16:20

... Xiaohua S, Xinyu R, Kai W, Tonghua L: Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines Mol Cancer ... Materials and methods Cell cultures and clinical samples BxPC-3 human pancreatic cancer cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Gibco BRL) 293T cells were maintained ... signal induces cell migration and invasion in pancreatic cancer Br J Cancer 2008, 99(2):305-13 27 Garkavtsev I, Kozin SV, Chernova O, Xu L, Winkler F, Brown E, Barnett GH, Jain RK: The candidate...

Ngày tải lên: 10/08/2014, 22:21

... augment inflammation and joint destruction in arthritic conditions In inflamed RA synovial tissue, macrophages and dendritic cells have now been shown to express FcγRIIb in addition to activating ... activating FcγR-expressing cells and T cells in RA synovial tissue [1] By enhancing the capacity of effector T cells to activate B cells as well as fibroblasts and osteoclasts (and macrophages and ... expression of inhibitory and activating FcγRs will lead to enhanced understanding of FcγR-mediated immunopathology in RA Apart from nonspecific modulation of the FcγR balance by existing or currently...

Ngày tải lên: 09/08/2014, 10:20

... Interleukin IL-10 Interleukin 10 JNK c-Jun N-terminal kinases MAPK Mitogen-activated protein kinase MAPKKK Mitogen-activated protein kinase kinase kinase xiv MKK3 Mitogen-activated protein kinase ... development of breast cancer Red boxes indicate increased risk, while green boxes indicate reduced risk 1.2 Breast cancer: chemoprevention and treatment Early signs of breast cancer include lump in breast ... and this type of cancer is more common in female population than male There are two types of breast cancer namely ductal carcinoma and lobular carcinoma Ductal carcinoma is originating from breast...

Ngày tải lên: 10/09/2015, 09:09

... that includes a DNA-binding domain, a ligand-binding domain and a transactivation domain The DNA-binding domain is responsible for DNA binding specificity and dimerization, and the ligand-binding ... domain involved in protein–protein interactions FYRC and FYRN domains are involved in heterodimerization between MLLN and MLLC terminal fragments High-mobility group (HMG) domains are involved in ... transformation Thus, diverse DNA-binding domains, protein–protein interaction modes and pre-existing chromatin modifications may facilitate the binding of MLLs, depending on the context and cell types, to facilitate...

Ngày tải lên: 16/02/2014, 14:20

... Materials and methods Animals and peptides Carcinus maenas were collected using baited traps in the Menai Strait, UK, and maintained in a recirculating seawater system under ambient conditions MIH and ... of MIH and CHH in the sinus gland during the moult cycle As individual SG from single crabs always contained almost identical profiles and quantities of peptides (preliminary experiments using pairs ... crabs in which CHH and MIH levels were individually measured and analyzed by ANOVA showed highly significant pairing in peptide contents between sinus glands from individuals, thus demonstrating...

Ngày tải lên: 21/02/2014, 00:20

... successfully inhibited the expression of VEGF, thereby suppressing the growth of hepatocarcinoma tumors in mice [87] In addition, no noticeable increase in inflammatory cytokines, including interferon-a and ... required [12] In this minireview, major factors in determining overall RNAi efficiency in vivo are introduced Moreover, up-to-date progress in achieving efficient and targeted siRNA delivery in vivo, ... obtained by in vitro transcription reaction and, therefore, avoid contamination by cell or bacterial products Promising in vitro and in vivo RNAi was obtained using siRNA that was directly linked...

Ngày tải lên: 06/03/2014, 01:23

... operates in the specific field of nursing, and the role the law has played not only in obstructing the professional development of nursing, but also in supporting and maintaining nursing’s continued ... care, and nursing in particular.Yet in spite of punishing workloads, decreased funding, and scepticism from colleagues, more and more nurses are finding ways of incorporating complementary therapies ... nursing, effects of complementary therapies on reducing stress and pain, and role of nurses and midwives in promoting healing, health and wellbeing • Seek out colleagues who are resistant and...

Ngày tải lên: 06/03/2014, 13:20

... the term “Highland” is known as mountainous regions in Vietnam, including mountainous regions in the North and Central highland The term “Lowland” is defined as deltas, hilly areas and coastal zone ... of human skin In the hot season, wind makes the process of sweat evaporation from skin easy However, strong wind in winter makes human body colder, strong and cold wind makes children and old people ... weather days in a year In other places, such as Co To Island, Tay Ninh, Ho Chi Minh City, there are about 200 good weather days per year For health resorts and some weather therapies in highland areas...

Ngày tải lên: 14/03/2014, 15:20

... continuing advances in technical and professional knowledge Certain principles remain just as important in diagnosis as they are in screening Training, multidisciplinary teamwork, monitoring and ... screening should be established in line with the routine screening interval, taking into consideration that most programmes not succeed in keeping the routine screening interval for each individual ... screening should be established in line with the routine screening interval, taking into consideration that most programmes not succeed in keeping the routine screening interval for each individual...

Ngày tải lên: 15/03/2014, 00:20

... ung th in vitro, thấy quercetin làm thoái giáng đột biến p53 - protein, làm ngừng chu kỳ tế bào giai đoạn G1 , ức chế tyrosin - kinase, ngăn chặn hoạt tính protein - ras In vitro, quercetin ức ... Curcumin có nhiều ảnh hởng đến sinh lý bệnh học tế bào ung th: in vitro, curcumin làm ngừng chu kỳ tế bào u, thúc đẩy chết theo chơng trình (apoptosis) Tuy nghiên cứu hạn chế, nhng thấy curcumin ... bào khác nhau, thấy quercetin làm tăng hiệu lực điều trị cisplatin, adriamycin, busulfan, cyclophosphamid làm tế bào ung th giảm đề kháng với gemcitabin topotecan Bromelain Là hỗn hợp protease chứa...

Ngày tải lên: 20/03/2014, 03:20

... stimulation, vaginal cones) for stress urinary incontinence in women published in the last decade (1995–2005) Background and rationale The International Continence Society defines urinary incontinence ... training in older women • Evidence for skill training was found, especially if combined with strength training in women of all ages, but the optimal specific training protocol for skill training ... http://www.biomedcentral.com/1472-6874/6/11 tion of incontinence with increasing age, effective training programs for older women are needed Initial severity of incontinence Previous studies have reported conflicting findings about...

Ngày tải lên: 28/03/2014, 14:20

... truncated BRCA1 or induce apoptosis in a cell line expressing mutated BRCA1 The data in Figs and suggest that TRAIL can alter BRCA1 localization and induce apoptosis in breast cancer cells Therefore, ... to increase We observed an increase in HIF-1a protein, and thus were confident that the cells were being maintained in a low-oxygen environment and were responsive to low oxygen There is also increasing ... Collectively, the data in Fig support the involvement of a TRAIL-dependent process in mediating hypoxiainduced changes in BRCA1 localization TRAIL, but not hypoxia, induces apoptosis in breast cancer cells...

Ngày tải lên: 30/03/2014, 03:20

... by our institutional review board obtained) was isolated and maintained in TIL Media I medium with 10% heat-inactivated FCS, mM L-glutamine, 100 U/ml penicillin, and 0.1 mg/ml streptomycin The ... assay Methods Cell lines K562 cells (American Type Culture Collection) were maintained in DMEM medium Colorectal carcinoma cell lines (COLP-2 and COLM-6) were maintained in TIL Media I medium ... saline, and underwent up to nine times vaccinations via SC injection in the left inguinal area at 2-week intervals [29,30] The number of DCs used for the generation of fusions was 1–2 × 106 in...

Ngày tải lên: 18/06/2014, 15:20