hla typing by direct dna sequencing

... DNA was isolated from 10 independent clones of Escherichia coli by a modified alkaline lysis procedure [12] and sequenced by using quick start cycle DNA sequencing kit (Beckman-Coulter) The DNA ... identified as belonging to genotype A by LiPA or DNA sequencing while the remaining 13 strains were identified as mixed genotype A and D strains by the other two genotyping methods The data showed that ... technically demanding, LiPA and direct DNA sequencing yielded accurate identification of all genotype A and genotype D and mixed genotype A and D strains of HBV The DNA sequencing data reported in...

Ngày tải lên: 12/08/2014, 04:20

... ladder by radioactive sequencing compared to fluorescent peaks DNA Representation 3.2 Automation and sample preparation Automated DNA sequencing instruments (DNA sequencers) can sequence upto 384 DNA ... Victor Llaca Chapter DNA Sequencing and Crop Protection 61 Rosemarie Tedeschi Chapter Improvement of Farm Animal Breeding by DNA Sequencing 85 G Darshan Raj Chapter The Input of DNA Sequences to ... the researchers in the field of DNA sequencing An overview of DNA sequencing technologies right from the Sanger’s method to the next generation high throughput DNA sequencing techniques including...

Ngày tải lên: 08/03/2014, 19:20

... Flock size No of animals sampled Microscopy Positive Seropositive PCR positive Blood Confirmed by DNA sequencing Serum June, July and August 2000 Fussingø, Central Jutland 38 lambs 31 samples from ... fluorescent antibody assay, IFA) by the National Veterinary Institute, Uppsala, Sweden Total DNA was extracted from whole blood or serum using the QIAGEN QIAamp DNA Blood Mini Kit based on the ... concentration of the eluted DNA should be 15-60 ng/μl (3-12 μg of DNA eluted in 200 μl of buffer AE) Due to low sensitivity in the PCR reactions modified methods for extraction of DNA were tested and...

Ngày tải lên: 12/08/2014, 18:22

... verified by DNA sequencing (ABI PRISM377 DNA sequencer; PerkinElmer Life Science, Wellesley, MA) Parental TMK-1 cells were transfected overnight with the bcl-XL vector or the empty pcDNA3.1 vector by ... expression vector pcDNA3.1 Plasmid DNA was prepared by standard techniques (QIAGEN Plasmid Midi Kit; Hilden, Germany) pBabePuro, a puromycin-resistant vector, was kindly provided by Dr K Shuai (University ... indicated times and used for the DNA fragmentation assay as described in Experimental procedures pretreatment (Fig 5C) By contrast, JunD expression was not induced by EGF treatment (data not shown)...

Ngày tải lên: 19/02/2014, 06:20

... inhibited by ndsDNA [20] In our case, at low [ndsDNA] and high [ATP], the opposite situation may arise, when ATP inhibits binding of ndsDNA and subsequent ligation by occupying the dsDNA-binding ... mixture by the concentration of T4 DNA ligase To study the T4 DNA ligase-promoted end-joining reaction, we used synthetic DNA oligonucleotides as described in Experimental procedures Two ndsDNA substrates ... of nick recognition by DNA ligase has been proposed [22] The key feature of the mechanism is the B-to-A DNA helix transition of the enzyme-bound dsDNA motif, which results in DNA contraction, bending,...

Ngày tải lên: 21/02/2014, 00:20

Ngày tải lên: 21/03/2014, 01:20

... substrate, and 10 U DNA ligase (Weiss units in the case of T4 DNA ligase) at 16 °C (T4 DNA ligase and E.coli DNA ligase) or 45 °C (Taq DNA ligase and 5997 Ligation of ssDNA by T4 DNA Ligase Ampligase) ... Ligation of ssDNA by T4 DNA Ligase nontemplated ligation on ssDNA substrates [29,30], this property of T4 DNA ligase has, to our knowledge, not been reported Unlike T4 DNA ligase, bacterial DNA ligases ... ssDNA by T4 DNA Ligase H Kuhn and M D Frank-Kamenetskii A B Fig Investigation of ssDNA ligation activity of different DNA ligases Oligonucleotide I (10 nM) was incubated with ligase, cleaved by...

Ngày tải lên: 23/03/2014, 11:20

... cytochrome P450BM3 by direct electrochemistry The holoenzyme response at a DDAB-modified EPG electrode was characterized by redox couples at )0.388 V and )0.250 V These were identified as being direct electron ... been shown to be useful in attaining direct electrochemistry of heme-containing proteins [17–22] In the present work we employed this method to obtain the direct, quasi-reversible electrochemistry ... Voltammograms were taken in solutions that had been deoxygenated by purified argon For buffered protein solutions this was accomplished by blowing argon over the solution for several hours An argon...

Ngày tải lên: 23/03/2014, 21:20

... by NO However, activation of sGC by NO is regulated by phosphorylation by cAMPdependent protein kinase and protein kinase C [21,22] Recently, a novel mechanism by which adenine nucleotides allosterically ... guanylate cyclases by interacting directly with the enzyme, rather than through a separate coupling protein Indeed, inhibition of purified sGC by 2-substituted nucleotides was not regulated by guanine ... allosteric regulation by those nucleotides is a generalized characteristic of the family of guanylate cyclases Allosteric inhibition by 2-substituted nucleotides is mediated by their direct interaction...

Ngày tải lên: 24/03/2014, 03:21

... to Ml3 prior to sequencing Sequencing reactions can be performed directly on plasmid DNA, the double stranded molecule being denatured prior to sequencing Recent innovations in DNA purification ... approach to chemical DNA sequencing by subcloning in pGV451 and derrved vectors Meth Enzym 155,23 l-250 Eckert, R L (1987) New vectors for rapid sequencing of DNA fragments by chemical degradation ... of shotgun sequencing by such a method was suggestedby a colleague, who, as a pioneer in DNA synthesis and its automation, saw a perfect match of this emerging technology with DNA sequencing...

Ngày tải lên: 11/04/2014, 09:40

... Introduction 1-1 Introduction to Automated DNA Sequencing Sanger Dideoxy DNA polymerases copy single-stranded DNA templates, by adding nucleotides to a Sequencing growing chain (extension product) ... current ABI PRISM DNA sequencing kits use cycle sequencing protocols See Chapter for information on cycle sequencing protocols Figure 1-3 Cycle sequencing Advantages of Cycle o Sequencing o Protocols ... ABI PRISM DNA sequencing kits 1-6 Introduction Applied Biosystems DNA Sequencing Instruments ABI 373 The ABI 373 DNA Sequencer is an automated instrument for analyzing uorescently DNA Sequencer...

Ngày tải lên: 02/06/2014, 09:23

... phases of Ni NPs were analysed by l-XRD measurements, which were made using a Bruker-AXS D8-Discover diffractometer, and the crystal phases of Co NPs were analysed by conventional XRD measurements, ... inspected by ESEM image analysis in order to confirm that the oxide barrier layer was entirely removed from the pore bottom tips Figure shows a set of ESEM images of the templates As we can see, by the ... interpillar distance can be established beforehand by modifying the anodization parameters (anodization voltage, acid and concentration mainly) By applying this technique with other methods for...

Ngày tải lên: 21/06/2014, 20:20

... GENOMIC DNA First, DNA has to be extracted from the cells of the organism being studied The sequencing reaction is then performed on the DNA, and the sequenced DNA strands are sorted by size ... length Viral and bacterial DNA sequences, which not contain introns, are typically the shortest genes 16 17 GENOMIC DNA The dideoxy DNA sequencing procedure was invented by Frederic Sanger and his ... objectives To understand how DNA is sequenced using the most common technique automated dideoxynucleotide sequencing Content INTRODUCTION GENOMIC DNA DNA PREPARATION SEQUENCING REACTION STRAND...

Ngày tải lên: 29/06/2014, 13:20

... dry matter basis) measured by the direct method of maize (4071 4400 kcal) The GE values of maize measured by the direct method were different from the ones by the indirect method of Ewan (1989) ... GE values by the direct method and the indirect method of maize were 1.4 - 6.2% The different maize varieties had their different ME values The ME values (on a dry matter basis) by the direct method ... The ME value of feedstuffs is determined on chicken by the direct methods (biological method) According to results of the direct methods, the indirect methods, which are based on the chemical compositions...

Ngày tải lên: 06/08/2014, 18:22

... snietorp 3PV dna ,4PV ,2PV eht gnidocne ,342PV-ANDcp ,eniccav AND ehT sdimsalp fo noitaraperp dna noitcurtsnoC doirep latnemirepxe eht gnirud mutibil da retaw dna doof htiw dedivorp dna erusserp ... ralucelom a htiw dnab a dna ,)1 giF( metsys noitceted dna noitalsnart /noitpircsnart ortiv ni na gnisu detceted saw γ-NFIhC-ANDcp yb desserpxe nietorp ehT iloc E la te noitalsnart dna noitpircsnart ... htiw dna γ-NFIhc 8.4 gnidocne dimsalp dna 342PV-ANDcp htiw :γ-NFIhc sulp eniccav AND ;egnellahc eht htiw dna NDO-GpC htiw dexim 342PV-ANDcp htiw :NDO -GpC sulp eniccav AND ;egnellahc eht htiw dna...

Ngày tải lên: 07/08/2014, 18:21

... design DNAdirected alkylating agents by linking the alkylating pharmacophore to the DNA- affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder) [7,8] In most cases, the DNA- directed ... http://www.ro-journal.com/content/6/1/7 DNA alkylating agents are used widely for treatment of a variety of pediatric and adult cancers because the cytotoxic effects of these agents can directly modify DNA and cause DNA lesions ... irradiation was obtained by the survival fractions measured by separate treatment as reported previously [21] The expected effect by two separate treatments was determined by the formula SF(Drug)...

Ngày tải lên: 09/08/2014, 09:20

... infection by specifically deaminating the first-strand cDNA synthesized by reverse transcriptase (reviewed in [15]) The human immunodeficiency virus HIV-1 evades this protective mechanism by expressing ... deposited research Knowing that AID induces transcription-coupled mutagenesis by deaminating C to U in DNA, and that uracil -DNA glycosylase removes U to produce an abasic site (see shaded box in Figure ... single-stranded DNA but requires the action of RNase Proc Natl Acad Sci USA 2003, 100:4102-4107 Chaudhuri J, Tian M, Khuong C, Chua K, Pinaud E, Alt FW: Transcription-targeted DNA deamination by the AID...

Ngày tải lên: 09/08/2014, 20:20

... recovered by both approaches [10] Using both techniques thus samples a greater fraction of the genome, and it seems possible that genes encoding microRNAs and small polypeptides will be captured by ... Placing sequences onto maps derived from bacterial artificial chromosome (BAC) contigs by hybridization or low-pass sequencing, would be appropriate Genome filtration may http://genomebiology.com/2004/5/5/223 ... Maize genome sequencing project Plant Physiol 2001, 127:1572-1578 Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, et al.: Initial sequencing...

Ngày tải lên: 09/08/2014, 20:20