histogram of toxic pathways affected by troglitazone treatment 117
Expression dynamics of the hepatic mitochondrial proteome of the sod2+ mouse in response to troglitazone administration
Ngày tải lên :
12/09/2015, 11:08
... terms of proteins in and weeks treatment 114 Figure 33 Schematic diagram of mitochondrial dysfunction after –weeks of troglitazone administration 116 Figure 34 Histogram of Toxic Pathways ... Histogram of Toxic Pathways affected by troglitazone treatment 117 Figure 35 Cluster analysis of detected of proteins show bimodal expression 118 Figure 36 Impact of troglitazone on HET electron ... mitochondrial toxic effects of troglitazone This is more compatible with the idiosyncratic DILI nature of troglitazone toxicity Such a model also explains the independence of correlation of drug dosage...
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Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
Ngày tải lên :
07/03/2014, 15:20
... This was first apparent by h poststimulation and levels of ICAM-1 protein continued to rise over the 18 h of the experiment To examine the role of NF-jB in the induction of ICAM-1, cells were infected ... prior to analysis by EMSA Following each treatment, two NF-jB DNA binding complexes were observed and these were both removed by competition with the addition of 100-fold excess of cold oligonucleotide ... PD098059 and U0126, were validated by analysis of phosphorylation of ERKs and 2, which lie downstream of MEK1 [24] Both TNFa and IL-1b upregulated phosphorylation of p42/44 ERKs and preincubation...
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Báo cáo y học: "Aciculatin inhibits lipopolysaccharide-mediated inducible nitric oxide synthase and cyclooxygenase-2 expression via suppressing NF-B and JNK/p38 MAPK activation pathways" pot
Ngày tải lên :
10/08/2014, 05:21
... or absence of aciculatin (1-10 μM) in RAW264.7 mouse macrophage cells Page of 11 Measurement of nitrite as an index of NO production was done by the Griess method A significant level of nitrite ... antiinflammatory agent Page of 11 Materials and methods Reagents and materials Aciculatin was extracted and purified by one of our colleagues (Dr Chien-Chih Chen) to a purity of greater than 98% by HPLC and ... Following the manufacturer’s protocol, a mixture of μL of TurboFect™ (Fermentas) and μg of plasmid DNA, pEGFP-N1 plasmid, or pGL4.74[hRluc/TK] vector in 100 μL of DMEM serum-free medium was incubated...
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Tài liệu Báo cáo khoa học: Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity docx
Ngày tải lên :
21/02/2014, 00:20
... [24–26] As the action of arsenite involves the modification of a limited number of arsenite-sensitive target proteins, we hypothesized that elucidation of the mechanism of arsenite-induced glucose ... (Fig 3B) Treatment with arsenite did not lead to phosphorylation of PKB on either Ser473 or Thr308, nor of phosphorylation of PKC-k on T403 This observation agrees with the absence of PI-3¢ kinase ... increases the uptake of 2-DOG without the need for PI-3¢ kinase activity as judged from the absence of an effect of arsenite on PI-3¢ kinase activation and the lack of inhibition by either wortmannin...
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Báo cáo y học: "Homocysteine-induced macrophage inflammatory protein-2 production by glomerular mesangial cells is mediated by PI3 Kinase and p38 MAPK" doc
Ngày tải lên :
11/08/2014, 08:22
... investigated role of MAPK and PI-3 Kinase in MIP-2 expression induced by Hcy Hcy-induced MIP-2 was significantly attenuated (p < 0.05) by a PI-3 Kinase inhibitor (LY294002) and by an inhibitor of a p38MAPK ... observations related to blunting of the effect of Hcy on MIP-2 by inhibitors of PI3 Kinase and p38MAPK, western blotting analyses was employed to determine levels of activated (phosphorylated) p38MAPK ... extracellular Hcy involves activation of MAPK, the role of MAPK activation in MIP-2 production by MC was evaluated Methods Cell Culture Sprague-Dawley rat MCs were isolated by the sieving method [18] The...
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báo cáo hóa học: " Air pollution & the brain: Subchronic diesel exhaust exposure causes neuroinflammation and elevates early markers of neurodegenerative disease" pdf
Ngày tải lên :
19/06/2014, 22:20
... pro-inflammatory effects of DE Subchronic DE Exposure Modifies the Pro-inflammatory Profile of the Midbrain In an effort to further address the degree of sensitivity of the midbrain to air pollution, ... precede evidence of neuropathology Future research needs to address the effects of lifetime air pollution exposure and the impact of aging on neuroinflammation and neurotoxicity List of abbreviations ... assessed for the presence of Tau [pS199] by ELISA (Invitrogen, Carlsbad, CA), per manufacturer instructions The amount of Ab42 was measured in frontal lobe samples by ELISA with the Human/Rat...
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Báo cáo y học: "Prolonged ozone exposure in an allergic airway disease model: Adaptation of airway responsiveness and airway remodeling" pptx
Ngày tải lên :
12/08/2014, 16:20
... for 4, from 12 Mean number of myofibroblastszone of specimens 8, andmice Mean number of myofibroblasts counted under electron microscopy in the subepithelial zone of specimens from mice exposed ... of airway remodeling in the lungs of ozone-exposed mice was assessed by histological examination of toluidine blue-stained sections of lung tissue The lungs of mice exposed to ozone for 4, 8, ... order by the same observer, who was unaware of the origin of the sections Intra-observer repeatability was assessed by measuring the same section four times and was expressed as a percentage of...
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Tài liệu Exposure to Artificial UV Radiation and Skin Cancer ppt
Ngày tải lên :
14/02/2014, 21:20
... Investigation by Funnel plot representation of a possible publication bias in the studies of risk for melanoma associated with first use of indoor tanning facilities in youth Standard error of logarithm of ... limiting emission of UVB and UVC to a certain extent depending on the thickness of the glass Exposure of individuals to these lamps was of short duration but could lead to the development of erythema, ... viewed by many as the treatment of choice for psoriasis (Honigsmann, 2001) Narrow-band UVB is thought to be more effective than broadband UVB and almost as effective as PUVA in the treatment of psoriasis,...
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Tài liệu Báo cáo khoa học: ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin docx
Ngày tải lên :
18/02/2014, 17:20
... its original activity upon chelation of Mg2+ by the addition of mm EGTA (Fig 1C) Clearly, the capacity of ATP to lower the peroxidase activity in the absence of exogenous components revealed a ... peroxidase activity, was not affected by the presence of 2.5 mm ATP These data provide the first evidence 1452 that the dual functions of 2-Cys Prx can be differentially regulated by ATP and Mg2+ The interaction ... structural features of 2-Cys Prx Given the essential role of ATP in the peroxidase activity, we evaluated changes in the structure of 2-Cys Prx brought about by the concerted action of the nucleotide...
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THE FINANCIAL CRISIS INQUIRY REPORT - Final Report of the National Commission on the Causes of the Financial and Economic Crisis in the United States docx
Ngày tải lên :
06/03/2014, 09:22
... Reserve Board, the Federal Reserve Bank of New York, the Department of Housing and Urban Development, the Office of the Comptroller of the Currency, the Office of Federal Housing Enterprise Oversight ... fueled by “voracious” Wall Street investment banks; he thus opted out of certain kinds of investments by . William Martin, the vice chairman and chief executive officer of Service st Bank of ... Florida might become part of dozens of securities owned by hundreds of investors—or parts of bets being made by hundreds more Treasury Secretary Timothy Geithner, the president of the New York Federal...
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Air pollution exposure estimation using dispersion modelling and continuous monitoring data in a prospective birth cohort study in the Netherlands potx
Ngày tải lên :
06/03/2014, 19:20
... assessment of annual average PM10 and NO2 concentrations, using a combination of the three Dutch standard methods The performance of this modelling procedure based on (a combination of) the three ... months) that are particularly of interest in pregnant women and children It also facilitates identification of critical windows of exposure These short-term exposure Page of 11 windows cannot be examined ... Acknowledgements The Generation R Study is conducted by the Erasmus Medical Center Rotterdam in close collaboration with the School of Law and Faculty of Social Sciences of the Erasmus University Rotterdam,...
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Exposure to ambient air pollution and prenatal and early childhood health effects potx
Ngày tải lên :
06/03/2014, 19:20
... effect of each outcome for every increase in the levels of TSP, PM10, SO2 and NOx of 10 lg/m3, and of mg/ m3 in the levels of CO detected, the ‘random effect model’ was applied For the purposes of ... third trimester of pregnancy and ethnicity OR by increase of lg/m3 in formaldehyde levels: annual exposure 1.36 (0.75–2.47); 1st trimester of pregnancy 2.39 (1.07–5.32) OR by increase of 10 lg/m3 ... means in each trimester of pregnancy of all daily measurements taken by all monitors in the district of birth of each infant: SO2 (32), NOx (38), TSP (72) Mean concentration of SO2 (8.0), hydrocarbons...
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Báo cáo khoa học: Molecular basis for specificities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases pptx
Ngày tải lên :
16/03/2014, 05:20
... total volume of 25 lL The amount of propionaldehyde formed was determined as described in the text The extents of reactivation of diol dehydratase by DDR and of glycerol by dehydratase by GDR were ... total volume of 25 lL The amount of propionaldehyde formed was determined as described in the text The extents of activation of diol dehydratase by DDR and of glycerol dehydratase by GDR were ... basis of the specificities of reactivating factors in the reactivation of inactivated holoenzymes and the activation of inactive enzyme–cobalamin complexes, the possibility of cross-formation of...
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Crosstalk between SET7/9-dependent methylation and ARTD1-mediated ADP-ribosylation of histone H1.4 pptx
Ngày tải lên :
22/03/2014, 18:20
... was found that ADP-ribosylation of H3 by ARTD1 prevents H3 methylation by SET7/9 However, PARylation of histone H3 surprisingly allowed subsequent methylation of H1 by SET7/9 Histone H1 was thus ... of histone H2A, K30 of H2B, K27 and K37 of H3 as well as K16 of H4 as ADP-ribose acceptor sites (catalyzed by ARTD1) [23] Crosstalk between different PTMs occurs directly by competition for acceptor ... However, after minute of PARylation of the histones by ARTD1, the methylation of core histones was already strongly reduced Figure PARylation inhibits SET7/9-dependent methylation of histone H3 and...
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Báo cáo khoa học: Mitochondrial biogenesis in mtDNA-depleted cells involves a Ca2+-dependent pathway and a reduced mitochondrial protein import pdf
Ngày tải lên :
23/03/2014, 15:21
... determined by the immunodetection of a-tubulin (C) Quantication of cyt c expression after optical density determination of the different signals and normalization by the abundance of a-tubulin ... depletion of mtDNA below a certain level as well as treatment of mammalian cells with respiratory inhibitors increased steady-state levels of cytosolic calcium that may change activities of several ... mtDNA-depleted cells This statement is also supported by the analysis of mitochondrial population abundance perfomed by the quantication of the cell area occupied by mitochondria on transmission electron...
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Báo cáo khoa học: PKCd-dependent cleavage and nuclear translocation of annexin A1 by phorbol 12-myristate 13-acetate pdf
Ngày tải lên :
23/03/2014, 21:20
... form of ANX-1 was detected in PMAstimulated HEK 293 cells, whereas only the intact form of ANX-1 was found in control cells ANX-1 was partially cleaved by the treatment of PMA and most of ANX-1 ... H.-B & Na, D.S (1996) Translocation of lipocortin (annexin) to the membrane of U937 cells induced by phorbol ester, but not by dexamethasone Br J Pharmacol 117, 1780–1784 14 John, C., Cover, P., ... cleavage of ANX-1 in HEK 293 cells ANX-1 translocates to the nucleus by PMA stimulation There have been reports that the cleaved form of ANX-1 is secreted and presents mainly on the outer surface of...
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Báo cáo hóa học: " Size-dependent catalytic and melting properties of platinum-palladium nanoparticles" doc
Ngày tải lên :
21/06/2014, 03:20
... melting temperature of the element i Hm is the size-dependent melting enthalpy of the element i The phase diagram of the Pt-Pd alloy is plotted in Figure We note that the lens shape of the phase diagram ... xliquidus − xliquidus e−( Hvap z1v ) (z1 kT) Table Number of (hkl) faces for each shape Shape Number of (111) faces Number of (100) faces Number of (110) faces Tetrahedron 0 Cube Octahedron 0 Decahedron ... melting enthalpy of nanomaterials Journal of Physical Chemistry C 2009, 113:3566 10 Liang LH, Zhao M, Jiang Q: Melting enthalpy depression of nanocrystals based on surface effect Journal of Materials...
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Báo cáo y học: "JNK pathway is involved in the inhibition of inflammatory target gene expression and NF-kappaB activation by melittin" ppsx
Ngày tải lên :
11/08/2014, 08:22
... examined by supershift assay using antibodies for the p50 or p65 components of NF-κB (data not shown) One of the consequences of inhibition of NF-κB is the inhibition of the nuclear translocation of ... translocation of p50 and p65 into the nucleus, we determined the appearance of the p50 and p65 in the nucleus extracts by Western blott Pretreatment (1 hr) of SP600125 suppressed the inhibitory effect of ... effect of melittin and bee venom on the generation of NO induced by LPS or SNP JNK inhibitor suppressed the inhibitory effect of melittin and bee venom on the generation of NO induced by LPS...
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Báo cáo y học: "Nicotine enhances murine airway contractile responses to kinin receptor agonists via activation of JNK- and PDE4-related intracellular pathways" pdf
Ngày tải lên :
12/08/2014, 11:20
... http://respiratory-research.com/content/11/1/13 and by in vitro results presenting activation of MAPK in human bronchial cells following stimulation of nicotinic receptors [9] Reports of a role for PDE4 inhibitors in asthma and COPD treatment ... of mitogen-activating protein kinases and NF-kappaB pathways Toxicol Sci 2008, 106(2):546-555 40 De Bie JJ, Hessel EM, Van Ark I, Van Esch B, Hofman G, Nijkamp FP, Van Oosterhout AJ: Effect of ... up-regulation of kinin receptors This idea is based on previous data revealing activation of MAPK-mediated NF-B inflammatory signal pathways in AHR along with an up-regulation of kinin receptors...
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Báo cáo y học: " RTE and CTE mRNA export elements synergistically increase expression of unstable, Rev-dependent HIV and SIV mRNAs" ppsx
Ngày tải lên :
13/08/2014, 09:21
... average of two to four plasmids per construct The data are presented in % of Gag production by normalizing the values produced by pNLgagRTEm26-CTE to 100% be explained by the 50% reduced activity of ... expression Results Synergistic activation of gene expression in the presence of a combination of RTE-CTE Since the presence of RTE or CTE positively affects production of poorly expressed retroviral genes, ... synergistic effect of RTEm26CTE is independent of splicing (data not shown) Analysis of total poly-A containing mRNAs from the transfected HeLa cells (Figure 1C) showed that the presence of either element...
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