high resolution gas chromatography olfactometry and mass spectrometry
Báo cáo y học: "Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS)" pps
Ngày tải lên :
13/08/2014, 15:21
... Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography- electrospray ... coupled with diode array detector and mass spectrometry (HPLCDAD-ESI-MS) and to assess the quality of two commercial mycelial AC products Methods Plant Powdered mycelium and an intact fruiting body ... cytidine, and thymine [10,11,17,18]; maleic acid and succinic acid derivatives [6,19,20]; benzenoids [21]; phenol and tocopherols [8,22]; 5’-nucleotides [14]; and diterpenes [23] No chemical standardization...
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Báo cáo y học: "Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry" pptx
Ngày tải lên :
13/08/2014, 15:21
... and 5-hydroxymethyl furfural; P-RPM contains less amino acids and monosaccharides and has a lower pH value than R-RPM [31] In recent years, high performance liquid chromatography (HPLC) and gas ... raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry Chinese Medicine 2010 5:29 Submit your next manuscript to BioMed Central and ... 10%, 35% and 100%, at 0, 45 and 65 minutes respectively Detection was performed at 280 nm The mass spectra were detected in positive mode The flow rate of drying gas (N2 ) and nebulizing gas were...
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Highly sensitive and accurate screening of 40 dyes in soft drinks by liquid chromatography–electrospray tandem mass spectrometry
Ngày tải lên :
25/08/2016, 22:30
... analysers was set on unit resolution Nitrogen was used as ion source gas 1, ion source gas 2, curtain gas and collision gas, with flow rates controlled at 65, 60, 25 and psi, respectively Ion ... into the mass spectrometer without flowsplitting The entire eluate was ionized simultaneously in positive and negative ionization mode, and monitored by SRM Mass selection for the Q1 and Q3 analysers ... ionization–tandem mass spectrometry (ESI-MS/MS) was used for screening The LC system was Agilent (Palo Alto, CA, USA) 1200 SL Series equipped with a binary pump, vacuum degasser, autosampler and thermostatic...
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Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
Ngày tải lên :
08/03/2014, 08:20
... constraints TIBTECH 15, 308–314 Klapa, M.I (2001) High resolution metabolic flux quantification using stable isotopes and mass spectrometry PhD Thesis, Massachusetts Institute of Technology, Cambridge, ... Klapa, M.I., Aon, J.C & Stephanopoulos, G (2003) Using iontrap mass spectrometry in combination with gas chromatography for high resolution metabolic flux determination Biotechniques 34, 832–840 ... carbons and and to a lesser extent of and of lysine that enables the estimation of the flux ratio at H4D using NMR measurements Finally, the irreversibility between the PEP and the PYR and OAA...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
Ngày tải lên :
15/03/2014, 00:20
... in 1% formic acid and stored at )20 °C until MS analysis High mass MALDI-TOF MS High- mass MALDI-TOF mass spectra of the intact protein complexes were obtained using a MALDI-TOF mass spectrometer ... treated with BS2G are seen in lanes and 8, and 11 and and 13, respectively Similar samples treated with BS3 are seen in lanes and 9, and 12 and and 14, respectively The arrows show the cross-linked ... protein bands resolved by SDS ⁄ PAGE and, corresponding to monomeric Ure2p, monomeric Ssa1p and Ure2p–Ssa1p complexes with apparent molecular masses of 120 and 160 kDa were excised Each protein band...
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Báo cáo y học: "Construction of a high-resolution genetic linkage map and comparative genome analysis for the reef-building coral Acropora millepora" ppt
Ngày tải lên :
09/08/2014, 20:20
... using 48 randomly chosen SNP markers, of which four came from the Orpheus Island and three from the Great Keppel Island, which are 80 km (NNW) and 570 km (SSE) away from Magnetic Island, respectively ... EM and MVM performed the cross and larval rearing SW and LZ conducted DNA preparation, SNP and microsatellite genotyping, and linkage analysis EM conducted comparative genome analysis SW, EM and ... randomly selected 48 SNP markers to test their applicability on colonies from Australian Great Barrier Reef locations, Orpheus Island (n = 4) and Great Keppel Island (n = 3), which are 80 km and...
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Báo cáo khoa hoc:" Rapid and specific influenza virus detection by functionalized magnetic nanoparticles and mass spectrometry" pdf
Ngày tải lên :
11/08/2014, 08:20
... gel electrophoresis (SDS-PAGE) and peptide mass sequencing using liquid chromatography tandem mass spectrometry (LC–MS/MS) Results and Discussion • Preparation and characterization of ethyleneglycol-protected ... microbalance [16], mass spectrometry [17–19], and microarrays [20–26] With the power of peptide sequencing and database searches for unknown protein identification, however, mass spectrometry has ... visualization and peptide sequence identification using liquid chromatography tandem mass spectrometry (LC–MS/MS) Conclusions: The assay combining efficient magnetic separation and MALDI–MS readout...
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Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx
Ngày tải lên :
12/08/2014, 18:22
... Pteronyssimus and D Farinae), molds (Cladosporium and Alternaria) and animal allergens (cat, dog and horse) SPT were performed on the volar side of the forearm with saline buffer as negative and histamine ... process with the existence of both positive and negative regulators that ultimately allow complete resolution and homeostasis In the absence of resolution and clearance or in the event of a dampened ... symptomatic birch and/ or grass pollen induced intermittent allergic rhinitis and 11 healthy volunteers (7 women), serving as controls The mean age of patients and controls was 43 (26–55) and 41 (24–55)...
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Báo cáo y học: "Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis" pdf
Ngày tải lên :
13/08/2014, 13:20
... liquid chromatography; LTQ: Linear ion trap mass spectrometer; MS: Mass spectrometry; MS/MS: Tandem mass spectrometry; RP: Reversed Phase; FT-ICR: Fourier Transformed Ion Cyclotron Resonance mass ... 50% ACN and 1.0% FA Mass Spectrometry Matrix-assisted laser desorption ionization (MALDI) tandem time-of-flight (TOF/TOF) mass spectrometry was carried out on an Applied Biosystems 4700 mass spectrometer, ... labeling and 2D-DIGE analysis KH, AZ and MD performed mass spectrometry data collection and data analysis KH, EP, AZ, EM, MD and AB drafted the manuscript FG carried out the western analysis KH, AB and...
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Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry
Ngày tải lên :
11/09/2015, 09:05
... (Baharvand et al., 2008) , the use of microscale twodimensional liquid chromatography and tandem mass spectrometry (2D LC-MS/MS) to identify ES cell-specific factors including Oct4, Sox2 and Utf1 ... 100 Tandem Affinity Purifications 117 Preparing Proteins for Mass Spectrometry 121 5.1 Identification of Purified Proteins by Mass Spectrometry 121 5.1.1 How MS and MS/MS ... instrumental in various ways, especially with protein work, mass spectrometry, and random jokes To members of the Lufkin lab past and present, especially Mathia, Sumantra, Serene, Song Jie, Yun,...
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analysis of proteins and protein complexes by chemical cross linking and mass spectrometry
Ngày tải lên :
30/09/2015, 05:51
... transform ion cyclotron resonance mass spectrometry HPLC high- performance liquid chromatography IAA iodoacetamide LC-MS/MS liquid chromatography tandem mass spectrometry LIT linear ion trap LRET ... (liquid chromatography tandem mass spectrometry) analysis Normally peptide mixtures are separated by reverse-phase chromatography and the eluted peptides are directly injected into a mass spectrometer ... cyclotron resonance mass spectrometry) and ESI-LIT-Orbitrap overcame this issue by acquiring CID MS/MS in a low resolution linear ion trap Q-TOF and Orbitrap also allow the high resolution CID MS/MS...
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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
Ngày tải lên :
22/03/2014, 14:20
... threshold 441 Present and < Mass 443 High- mass resolution 442 Base peak or >50% of Mass 198 High- mass resolution and sensitivity 443 15-24% of Mass 442 High- mass resolution and isotope ratio All ... peak Low -mass sensitivity 68
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Báo cáo sinh học: "High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry" docx
Ngày tải lên :
06/08/2014, 18:21
... several ion masses in parallel, has a high mass resolution (mass/ change in mass ratio of approximately 10,000) at high secondary-ion relative transmission (70-80%), and has a high lateral resolution, ... 13C- and 12C1H- (mass 13.003355 and mass 13.007825, respectively; Figure 15b); 12C14N- and 1H12C13C (mass 26.003074 and mass 26.011180, respectively; Figure 15c); 12C15N-, 13C14N- and 12C14N1H- (mass ... lateral resolution that reaches 33 nm, in theory, and a high mass resolution (a mass/ change in mass ratio of approximately 10,000) at high secondary-ion transmission (70-80%) allows us to image and...
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Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
Ngày tải lên :
05/09/2013, 10:15
... and Environment Technology, Vol 8, No.4, 2010 chromatography (Nowack et al., 1996; Kemmei et al., 2009), ion chromatography/ mass spectrometry (Bauer et al., 1999; Knepper et al., 2005), gas chromatography/ mass ... chromatography/ mass spectrometry analysis Gas chromatography was carried out by using HP6890 series gas chromatography system (Hewlett Packard, Wilmington, DE, USA) with an HP6890 series auto sampler and ... 1999; Knepper et al., 2005), gas chromatography/ mass spetrometry (Nishikawa and Okumura, 1995), and liquid chromatography/ tandem spetrometry (Quintana and Reemtsma, 2007) To simplify Japan’s recommended...
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Khối Phổ Phân Giải Cao HRMS (High Resolution Mass Spectrometry) và ứng dụng
Ngày tải lên :
02/05/2014, 23:47
... importance of mass uncertainty and resolving power" J Am Soc Mass Spectrom 16 (2): 183–98 Bossio RE, Marshall AG (April 2002) "Baseline resolution of isobaric phosphorylated and sulfated peptides and nucleotides ... toward mass spectrometry- based proteomics" Anal.Chem 74 (7):1674–9 He F, Hendrickson CL, Marshall AG (February 2001) "Baseline mass resolution of peptide isobars: a record for molecular mass resolution" ... of the two most common frequency-to -mass calibration functions for Fourier transform ion cyclotron resonance mass spectrometry" .International Journal of Mass Spectrometry 195-196: 591 doi:10.1016/S1387-3806(99)00226-2...
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báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc
Ngày tải lên :
20/06/2014, 00:20
... internal standard and b) without internal standard Figure the corresponded product the deuterated standards with Standard chromatogram of ion scans Standard chromatogram of the deuterated standards ... Leukotrienes LTB4 and LTE4as analytical standards and the labelled [2H4] LTB4 and [2H4] PGE2 as internal standards were purchased from Cayman Chemicals Company (Michigan, USA) All analytical standards ... specificity and detection related to structural information such as mass spectrometry Our developed method allows for a sensitive, specific and reliable determination of leukotriens and prostaglandins...
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Gas Chromatography Mass Spectrometry doc
Ngày tải lên :
10/08/2014, 17:22
... ghi.Các tín hiệu xử lý chuyển sang phận in lưu kết Process Flow Schematic Sample injection Carrier gas (nitrogen or helium) Long Column (30 m) Detector (flame ionization detector or FID) Air Hydrogen ... parapin, mỡ apiazol, polyetylen Các loại Silicon : polysiloxan – sản phẩm trùng ngưng monome silandiol R2Si(OH)2 Các loại ete, ester : phlatat, sebacat, photphat, suxinat, adipat dạng monome polime ... tìm kết tương ứng) Hoặc sở để đăng ký chất (nếu phép so sánh không tìm kết tương ứng) Hình 2: Mass- spectrum Trong hình 2, hình ảnh khối cao dodecane Phần nềm GC/MS giống thư viện hình...
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Báo cáo y học: "Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry." ppsx
Ngày tải lên :
13/08/2014, 15:21
... electrospray mass spectrometry; LC-ESI/MS: liquid chromatography- electrospray mass spectrometry; HPLC-DAD: highperformance liquid chromatography- diode array detection; MS/MS: tandem mass spectrometry; ... Negative Ion Liquid Chromatography Electrospray Ion Trap Mass Spectrometry J Am Soc Mass Spectrom 2001, 12:707-715 Stobiecki M: Application of mass spectrometry for identification and structural studies ... times of 5.5 and 8.0 minutes were isonaringin and naringin respectively (Figure 3B) Mass spectrometric structure characterization of naringin and isonaringin From the first order mass spectrometry...
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