heparan sulfate proteoglycans as multifunctional cell regulators cell surface receptors

... trypsin, collagenase and hyaluronidase as described previously [27] Sertoli cells were seeded at the concentration of 250 000 cellsácm)2 in 24-well dishes or in 75-cm2 plastic ¯asks and cultured ... aromatase mRNA was evaluated using semi-quantitative RT-PCR In the presence of 100 ngámL)1 FSH, cytochrome P450 aromatase mRNA expression was highly increased as described previously [30] Sertoli cells ... was not observed (Table 3) In conclusion, bFGF requires cell surface HSPG for the inhibition of steroidogenesis in developing Sertoli cells Cell surface HSPG are bFGF targets during Sertoli cell...

Ngày tải lên: 21/02/2014, 03:20

... cell- surface immobilized CXCL12 in endotheliawas sensitive to heparitinase, which selectively degrades the glycosaminoglycan moiety (heparan sulfate) in heparan sulfate proteoglycans (HSPGs) These findings ... Original magnification × 400 cells was performed by treatment with a cocktail of 100 mU/ ml heparitinases I, II and III each, or 100 mU/ml chondroitin sulfate ABC lyase as a control for 90 minutes ... pretreated with heparitinases (b, c) or sodium chlorate (d) In (b), surface heparan sulfate proteoglycan (HSPG) was detected with 10E4 mAb in RA ECs pretreated or not with heparitinases (e) RA ECs were...

Ngày tải lên: 09/08/2014, 07:20

... generation was measured by 2',7'dichlorofluorescein (DCFH) metabolism Neutrophils were gated based on FSC versus SSC parameters and the expression of CD14 on cell surface ROS generation was analyzed ... generation was measured by 2',7'dichlorofluorescein (DCFH) metabolism Monocytes were gated based on FSC versus SSC parameters and the expression of CD14 on cell surface ROS generation was analyzed ... and CD11c, integrins involved in adhesion with endothelial cells, as well as on signaling for LPS [30] Neutrophil activity was further assessed by ROS generation, Figure Figure across the continuum...

Ngày tải lên: 13/08/2014, 10:20

... basis for this dichotomous behavior of RIP1 was explained by the fact that when RIP1 is ubiquitinated it does not associate with caspase 8, whereas when it is not ubiquitinated, it rapidly associates ... RIP1, TNFR1-associated via death domain (TRADD), TRAF2 are recruited to the TNFR1 trimers in the plasma membrane early after receptor ligation whereas the cell death regulators FAS-associated ... TNF-mediated cell death in this situation Instead, the cells switch from caspase-dependent apoptosis to a cell death program with necrotic morphology [51] The kinase activity of RIP1 has been known...

Ngày tải lên: 28/03/2014, 23:20

... to drive the yeast internal invertase to the cell surface These cell biology data were further supported by the revalidation of our enzymatic assay of acid trehalase on intact cells, confirming ... confirming that glucose measured in NaF-treated intact cells results from the cleavage of the disaccharide at the cell surface by an extracellular ‘acid trehalase’ pool [14] The cell- surface localization ... place in this subcellular compartment In contrast, targeting this enzyme at the cell surface is indispensable for growth of yeast cells on trehalose Ath1 domains relevant for cell- surface targeting...

Ngày tải lên: 18/02/2014, 06:20

... the surface of myotubes (arrow in Fig 6D), as well as on the surface of nonmyosinexpressing cells Some of the punctate localization also occurred at the cell cell interface between these cells ... also reach cell surfaces without endogenous HSPG production [59], and nucleolin has even been reported to function as a shuttle between the cell surface and the nucleus [65] Our study has now shown ... and incubated with the cells for h at room temperature Cells were washed, mounted and analyzed as described above To semipermeabilize cells for intracellular staining, cells were incubated in...

Ngày tải lên: 19/02/2014, 05:20

... Imidazoline Receptors 6.8 Receptors of the Plasminogen–Plasmin Cascade 6.8.1 Urokinase-Type Plasminogen Activator Receptor 6.8.2 Plasminogen Receptors ... introduces cell- surface receptors to various ligands, details intracellular receptors and their translational partners, reports on some sets of plasmalemmal receptors (guanylate cyclase receptors ... organs such as the uterus or act locally as auto- or paracrine regulators Cells receive information from themselves and their environment via proteic receptors of the cell surface Cells appropriately...

Ngày tải lên: 05/03/2014, 22:21

... receptor, which has N-glycosylation sites at residues Asn251, Asn317 and Asn727 Mutation of Asn727 decreases cell- surface expression, whereas mutation at the other two sites does not [42] HFE ... that in certain cell types HFE acts to regulate intracellular iron levels by decreasing the binding of transferrin to transferrin receptors and reducing cellular iron uptake as a consequence ... mutation associated with HH leads to the replacement of histidine at residue 63 with aspartate This mutant is capable of b2M interaction and cell- surface expression but is unable to regulate cellular...

Ngày tải lên: 06/03/2014, 22:21

... Woessner, J.F Jr (2000) Heparan sulfate proteoglycans as extracellular docking molecules for matrilysin (matrix metalloproteinase 7) J Biol Chem 275, 4183–4191 42 Sopata, I & Maslinski, S (1991) Activation ... Chondroitin ABC lyase (proteinase free) was purchased from Seikagaku Kogyo Co CDP-StarTM chemiluminescent substrate was obtained from New England Biolabs Unlabeled molecular mass standards were ... activation was also reflected by the fact that the activation was equally as effective at 100 mM as at 10 mM of calcium The metal-induced activation of the MMP-9/ CSPG complex was specific for calcium as...

Ngày tải lên: 17/03/2014, 10:20

... cells in the presence of PEDF The cell- surface ATP synthase activity was measured in HMVECs treated with PEDF for the indicated periods of time Extracellular ATP generation was assayed Extracellular ... for F1-ATPase binding to angiostatin (A) or PEDF (B) surfaces was performed F1-ATPase (100 nM) was premixed with increasing concentrations of PEDF (as indicated), and injected onto each surface ... PEDF surface- binding assay Background baseline noise was slightly adjusted, using drifting baseline or accounting mass transfer limitation, to produce the best fit Radioligand-binding assays Assays...

Ngày tải lên: 22/03/2014, 21:21

... sLDL-R was released from PMAtreated and untreated HepG2 cells was assessed as shown in Fig Cells treated with PMA (closed symbols) released sLDL-R at a rate significantly faster than untreated cells ... proteases such as ADAM 10 and ADAM 17 (TNF-a converting enzyme or TACE) [34] From protease inhibitor studies these proteinases fall into two main classes: (a) elastase-like serine proteinases as ... Transferrin receptors and asialoglycoprotein receptors also display a decrease in cell surface number in response to PMA treatment of HepG2 cells [44] In the case of the latter two receptors, ...

Ngày tải lên: 23/03/2014, 12:20

... PTN requires a cell surface component other than heparan and chondroitin sulfate proteoglycans Described as a HB-GAM, PTN interacts with glycoaminoglycans such as heparan sulfate proteoglycans ... cytomegalovirus [23] Several cell surface components have been reported as potential receptors for PTN, such as the heparan sulfate proteoglycans of N-syndecan [24] and the chondroitin sulfate proteoglycan ... control CHO cell lines is decreased by 50 and 80% in CHO 677 and CHO 618 cells, respectively (Fig 3) This decrease is probably due to the lack of heparan sulfate and heparan ⁄ chondroitin sulfate...

Ngày tải lên: 30/03/2014, 20:20

... expression, cells expressed a similar amount of CXCR4 as Jurkat cells without the Env expression plasmid (Fig 5A–D) In contrast, a decrease in the level of CXCR4 expression was seen in >95% of Jurkat cells ... population of cells (

Ngày tải lên: 20/06/2014, 01:20

... respiratory epithelial cell membranes Heparinase III treatment efficiently removes heparan sulfate Heparinase III treatment efficiently removes heparan sulfate from respiratory epithelial cell membranes ... removes heparan sulfate from human tracheal epithelial (HTE) cell membranes Heparinase Heparinase III treatment efficiently removes heparan sulfate from human tracheal epithelial (HTE) cell membranes ... produce three times as much chondroitin sulfate as wild-type CHO-K1 cells However, removal of cell membrane chondroitin sulfate from human tracheal epithelial cells with chondroitinase ABC did not...

Ngày tải lên: 20/06/2014, 01:20

... Glycosidase sensitivity of cell surface transported Lassa virus Figure glycoprotein Glycosidase sensitivity of cell surface transported Lassa virus glycoprotein At 48 h post-transfection, Vero cells ... also transported to the cell surface of SKI-1/S1P deficient cells (data not shown) The finding that only an endo H-sensitive form of Lassa virus GP-C was found at the cell surface suggests that ... been shown to exhibit regular cell surface transport, the two reported cases in which cell surface expression was abolished might be due to misfolding in the endoplasmic reticulum with subsequent...

Ngày tải lên: 20/06/2014, 01:20

... culture-derived OC cells (data not shown) Differentiation of OC derived cells into osteoblasts was induced in vitro by treating the cells with ascorbic acid, βglycerophosphate and dexamethasone [2,20,21] ... adherence and cultivation The change in cellular morphology and the acquisition of a fibroblastic shape became increasingly apparent as the cells were cultured on plastic In the primary culture, nonadherent ... immunoreactive cells was quantified by flow cytometry Data from adherent, culture expanded cells were collected and the number of triple-positive events was expressed as a percentage of the total cell...

Ngày tải lên: 09/08/2014, 01:23

... monomeric proteins and surface plasmon resonance The ectodomain of Unc5b was expressed as a secreted Cd4d3+4-6His-tagged protein using mammalian cells, purified and eluted as a monodisperse peak ... using an independent technique, either surface plasmon resonance (Figure 3) or a bead-binding assay (Additional data file 2) IgSF -receptors = blue nodes, LRR -receptors = red nodes All interactions ... accounts for the vast majority (approximately 80%) of this class of LRR neuroreceptors in the zebrafish genome To identify novel interactions, we used the AVEXIS (for AViditybased EXtracellular Interaction...

Ngày tải lên: 09/08/2014, 20:20

... Sindbis virus to cell surface heparan sulfate J Virol 1998, 72:7349-7356 Klimstra WB, Ryman KD, Johnston RE: Adaptation of Sindbis virus to BHK cells selects for use of heparan sulfate as an attachment ... plaque assay was performed on BHK-21 cells as previously described [16] (b) Confluent BHK-21 cell monolayers were treated with heparinase I at the concentrations as indicated After washing three ... Hascall VC, Norcross MA: Cell- surface heparan sulfate proteoglycan mediates HIV-1 infection of T -cell lines AIDS Res Hum Retroviruses 1993, 9:167-174 12 Summerford C, Samulski RJ: Membrane-associated...

Ngày tải lên: 12/08/2014, 01:21

... MOI 0.05 TCID50 /cell of unpurified virus incubated as described in A) and the amount of virus bound to the cell surface was measured with a two-step RT-PCR Results are presented as mean ± SEM Page ... EV7W and CVB5 was incubated with 2.5 × 105 cells in suspension for h at 4°C and virus attachment was subsequently measured using RT-PCR B) MOI TCID50 /cell of CVB2O was incubated as described in ... CHO-CAR was observed when measuring interactions at the cell surface using purified viruses (Figure 1A) CVB prototype strains have been shown to utilize CAR as receptor [15,34] Several low-passage...

Ngày tải lên: 12/08/2014, 04:21

... 3, 4) CasE#1 CasE#1 efficiently infected M dunni cells (Xpr1sxv) and M pahari cells (Xpr1p) as well as rat and mink cells, but failed to infect hamster cells, NIH 3T3 (Xpr1n) and cells carrying ... [16,17] X/PMV CasE#1 Lake Casitas, California wild mouse IUdR treated embryo cells [14] X/PMV Cz524 CZECHII/ EiJ Spleen of month old inoculated with MoMLV This report A-MLV 4070A Lake Casitas, California ... 4.5+/-0.9 as the number of cells positive for β-galactosidase activity in 100 ul of virus Where no SD is given, infectivity was only tested once 0, no positive cells in cultures infected at least times...

Ngày tải lên: 12/08/2014, 23:22