heat shock genes and proteins

... evolutionary and ecological role of heat shock proteins Ecol Lett 6, 10251037 Parsell DA & Lindquist S (1993) The function of heat- shock proteins in stress tolerance: degradation and reactivation ... from heat shock and neurodegeneration Genetics 172, 275286 61 Li ZW, Li X, Yu QY, Xiang ZH, Kishino H & Zhang Z (2009) The small heat shock protein (sHSP) genes in the silkworm, Bombyx mori, and ... Tsumuki H (2006) Cloning and nucleotide sequencing of three heat shock protein genes (hsp90, hsc70, and hsp19.5) from the diamondback moth, Plutella xylostella (L.) and their expression in relation...

Ngày tải lên: 06/03/2014, 09:22

... infection or heat shock Stress conditions like heat shock, infections, radiation, and exposure to chemicals induce increased levels of heat shock proteins in many cell lines [9] The heat shock proteins ... 24 h or heat shock treated (44°C, 20 min) or heat shock treated and recovered for or h at 37°C (shift assay) Lane – control cells, lane – heat shock treated, lane – heat shock treated and recovered ... RT-PCR1analysis of the heat shock factor blood macrophages RT-PCR analysis of the heat shock factor and heat shock proteins in vaccinia virus infected human blood macrophages HSF1, HSP70, HSP90α and β-actin...

Ngày tải lên: 11/08/2014, 08:21

... EA: The heat- shock proteins Annu Rev Genet 1988, 22:631-677 Ang D, Liberek K, Skowyra D, Zylicz M, Georgopoulos C: Biological role and regulation of the universally conserved heat shock proteins ... McMillan DR: Stress (heat shock) proteins: molecular chaperones in cardiovascular biology and disease Circ Res 1998, 83:117-132 Huber SA: Heat- shock protein induction in adriamycin and picornavirus-infected ... 25:1727-1732 20 De Maio A: Heat shock proteins: facts, thoughts, and dreams Shock 1999, 11:1-12 21 Singleton KD, Serkova N, Beckey VE, Wischmeyer PE: Glutamine attenuates lung injury and improves survival...

Ngày tải lên: 13/08/2014, 03:20

... Vpr Ctr Heat shock factor is responsible for Hsp16 elevation and heat shock- mediated suppression of the Vpr activities Figure Heat shock factor is responsible for Hsp16 elevation and heat shock- mediated ... life cycle and pathogenesis For example, Vpr is required both in vitro and in vivo for viral pathogenesis and efficient viral infection of non-dividing host cells such as monocytes and macrophages ... was examined hrs after the heat shock, i.e., 23 hrs after vpr gene induction (GI) (B) Comparison of the Hsp16 protein levels between acute heat shock and constant heat treatment shown by Western...

Ngày tải lên: 13/08/2014, 09:20

... CJCGM (1999) Dementia, gliosis and expression of the small heat 2625 Small heat shock proteins and disease 120 121 122 123 124 125 126 127 128 shock proteins Hsp27 and [alpha]B-crystallin in Parkinson’s ... potential and decrease cell protection by Hsp25 In another example, Hsp27 and aB-crystallin appear in Parkinson’s disease 2617 Small heat shock proteins and disease with severe dementia [119] sHSPs and ... HSPs that protect deregulated intracellular signaling proteins and transcription factors 2619 Small heat shock proteins and disease Y Sun and T H MacRae Table sHSP modifications associated with...

Ngày tải lên: 19/02/2014, 18:20

... Genealogy of the a-crystallin-small heat- shock protein superfamily Int J Biol Macromol 22, 151–162 Sun, W., Van Montagu, M & Verbruggen, N (2002) Small heat shock proteins and stress tolerance in plants ... Chaperone activity and homoand hetero-oligomer formation of bacterial small heat shock proteins J Biol Chem 275, 37212–37218 Lentze, N., Studer, S & Narberhaus, F (2003) Structural and functional ... a -heat shock protein J Mol Biol 328, 927– 937 Studer, S., Obrist, M., Lentze, N & Narberhaus, F (2002) A critical motif for oligomerization and chaperone activity of bacterial a -heat shock proteins...

Ngày tải lên: 23/03/2014, 12:20

... time of heat shock, plated and CFU counted The proportions of viable cells expressing the pET 16b-MTB HSP 16.3 vector and the two control vectors were plotted for 0, and h following heat shock ... with and without MTB HSP 16.3 and aB-crystallin shifted to 48 °C The proportion of viable cells that survived heat shock at 48 °C was plotted at three time points (t ¼ 0, and h after 48 °C heat shock) ... (2000) Small heat- shock proteins and their potential role in human disease Curr Opin Struct Biol 10, 52–59 Landry, J., Chretien, P., Lambert, H., Hickey, E & Weber, L.A (1989) Heat shock resistance...

Ngày tải lên: 23/03/2014, 21:21

... structure and function of the small heat shock proteins in plants J Exp Bot 47, 325–338 Arrigo, A.-P & Landry, J (1994) Expression and Function of the Low-molecular-weight Heat Shock Proteins ... HspD, HspE and HspH) and two class B proteins (HspC and HspF), as well as the class A E coli proteins IbpA and IbpB A representative example Ó FEBS 2002 from each class, A (HspH) and B (HspF), ... Jong, W.W (2002) Evolution and diversity of prokaryotic small heat shock proteins Prog Mol Subcell Biol 28, 1–17 41 Narberhaus, F (2002) a-Crystallin-type heat shock proteins: Socializing minichaperones...

Ngày tải lên: 31/03/2014, 21:21

... regulating the expression of heatinducible genes other than classical heat shock genes Expression of nonclassical heat shock genes induced by HSFs Although the heat shock response was originally ... We now refer to these heat- inducible genes that are different from the classical heat shock genes as ‘nonclassical heat shock genes As already discussed, chicken HSF1 and mouse HSF3 play a substantial ... the classical heat shock genes, but had a significant effect on the protection of cells from heat shock [28] This effect was not mediated through the induction of classical heat shock genes or regulation...

Ngày tải lên: 18/02/2014, 04:20

... HSF3 translocates into the nucleus upon heat shock and may activate nonclassical heat shock genes, but does not activate classical heat shock genes (i.e HSP genes) HSF1 prefers long arrays of the ... achieved by HSF1 and the histone deacetylases HDAC1 and HDAC2 [77] In heatshocked cells, HSF1 binds to HDAC1 and HDAC2, and their histone-deacetylase activities increase The heat- induced deacetylation ... activate non-classical heat shock genes during heat shock Mol Biol Cell 21, 106–116 Trinklein ND, Murray JI, Hartman SJ, Botstein D & Myers RM (2004) The role of heat shock transcription factor...

Ngày tải lên: 18/02/2014, 04:20

... R Abane and V Mezger 113 Trinklein ND, Chen WC, Kingston RE & Myers RM (2004) Transcriptional regulation and binding of heat shock factor and heat shock factor to 32 human heat shock genes during ... Christians E, Michel E & Renard JP (1997) Developmental control of heat shock and chaperone gene expression Hsp 70 genes and heat shock factors during preimplantation phase of mouse development ... provided a mechanism for HSF1dependent heat shock- induced cell death in spermatocytes [86] Heat shock does not trigger the induction of major heat shock genes in male germ cells The prominent...

Ngày tải lên: 18/02/2014, 04:20

... mitochondrial depolarization [33,34] and impaired clearance of oxidized proteins [35] Heat shock or stress proteins (Hsps) are expressed in neurons of polyQ diseased brains and have recently been identified ... polyglutamine-expanded proteins Hum Mol Genet 12, 2301–2309 31 Ding Q & Keller JN (2001) Proteasome inhibition in oxidative stress neurotoxicity: implications for heat shock proteins J Neurochem ... aggregation and toxicity Proc Natl Acad Sci USA 99, 16412–16418 39 Wyttenbach A, Carmichael J, Swartz J, Furlong RA, Narain Y, Rankin J & Rubinsztein DC (2000) Effects of heat shock, heat shock protein...

Ngày tải lên: 19/02/2014, 06:20

... cycles of 0.5 at 94 °C, 0.5 at 50 °C and 0.5 at 72 °C, and one termination cycle of at 72 °C, with 35 cycles in total for HSF1a and HSF1b and 30 for heat shock cognate 70 (HSC70) Rainbow trout ... observed gel shift bands in the lanes containing epitopetagged HSF1 (Fig 6B, lanes and 8) The bands were detected at a position corresponding to the gel-shift band of heat- shocked RTG-2 cell extract ... Biology of Heat Shock Proteins and Molecular Chaperones Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York Wu, C (1995) Heat shock transcription factors: structure and regulation...

Ngày tải lên: 19/02/2014, 12:20

... D Mittal et al Heat shock element types and heat shock factors in rice Introduction The synthesis of heat shock proteins (Hsps) represents one of the most thoroughly ... 695 genes G-type and 1584 genes S-type HSEs in the rice genome (Fig 1A) In total 711, 476 and 1368 genes contained exclusively the P-, G- and S-type HSEs, respectively; 59 genes showed both P- and ... substrate and by filter lift assay (Yeast Protocols Handbook; Clontech Laboratories Inc., Mountain View, CA, USA) Each experiment was repeated three times Heat shock element types and heat shock factors...

Ngày tải lên: 05/03/2014, 23:20

... Structure and properties of the small heat shock proteins (sHsp) and their interaction with cytoskeleton proteins Biochemistry (Mosc) 67, 511–519 Macario AJL (1995) Heat- shock proteins and molecular ... Small heat- shock proteins and their potential role in human disease Curr Opin Struct Biol 10, 52–59 Renkawek K, Stege GJJ & Bosman GJCGM (1999) Dementia, gliosis and expression of the small heat shock ... small heat shock proteins hsp27 and aB-crystallin in Parkinson’s disease NeuroReport 10, 2273–2276 10 Head MW & Goldman JE (2000) Small heat shock proteins, the cytoskeleton, and inclusion body...

Ngày tải lên: 07/03/2014, 04:20

... Small heat shock proteins and stress tolerance in plants Biochim Biophys Acta 1577, 1–9 Horwitz J (2003) Alpha-crystallin Exp Eye Res 76, 145–153 Laksanalamai P & Robb FT (2004) Small heat shock proteins ... concepts and emerging ideas Cell Mol Life Sci 57, 899–913 Scharf K-D, Siddique M & Vierling E (2001) The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins ... (2004) CHIP and Hsp70 regulate tau ubiquitination, degradation and aggregation Hum Mol Genet 13, 703–714 MacRae TH (2000) Structure and function of small heat shock ⁄ a-crystallin proteins: established...

Ngày tải lên: 07/03/2014, 12:20

... class II small heat shock proteins Plant Physiol 114, 1477–1485 Basha, E & Vierling, E (2003) Cloning and sequencing of a new cytosolic class II small heat shock protein from wheat Egypt J Biotechn ... a-crystallin-small heat- shock protein superfamily Int J Biol Macromol 22, 151–162 Studer, S & Narberhaus, F (2000) Chaperone activity and homoand hetero-oligomer formation of bacterial small heat shock proteins ... small heat shock proteins with unfolding proteins J Biol Chem 278, 18015–18021 Nover, L., Scharf, K.-D & Neumann, D (1983) Formation of cytoplasmic heat shock granules in tomato cell cultures and...

Ngày tải lên: 07/03/2014, 15:20

... [12,13] As a major stress-inducible heat shock protein, heat shock protein (HSP) 70 has been shown to protect cells from a number of apoptotic stimuli, including heat shock, tumor necrosis factor, ... expression at a level similar to that of the endogenous proteins induced by heat shock This system mimics the antiapoptotic effects of heat shock and is very instrumen2618 tal with respect to our investigation ... activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation of caspases-9 and -3 were analyzed Cells over-expressing HSP70 and its deletion...

Ngày tải lên: 16/03/2014, 01:20

... high-molecular-mass heat shock proteins and 42 degrees C-specific heat shock proteins of murine cells Biochem Biophys Res Commun 204, 357–365 Yasuda K, Nakai A, Hatayama T & Nagata K (1995) Cloning and expression ... stresses such as H2O2, heat shock and anticancer drugs [17] In addition, Hsp105a suppresses protein aggregation and apoptosis caused by the expression of proteins with expanded polyQ tracts in ... antiapoptotic proteins Bcl-2 and Bcl-XL and inhibits their prosurvival function [36–39] In addition to Bcl-2 and Bcl-XL, previous studies have also implicated the proapoptotic BH3-only proteins Bid and...

Ngày tải lên: 16/03/2014, 06:20

... numbered and the b5 and b7 strands are shown in red and blue, respectively G104 (equivalent to K137 of human HSP22) and R108 (equivalent to K141 of human HSP22) are shown in purple and grey, ... 30 column For clarity, elution profiles of unheated and heated proteins are shifted from each other by 10 mAu and elution profiles between different proteins are shifted from each other by 30 mAu ... Rekas A, Thorn DC & Wilson MR (2003) Small heat- shock proteins and clusterin: intra- and extracellular molecular chaperones with a common mechanism of action and function? IUBMB Life 55, 661–668 FEBS...

Ngày tải lên: 23/03/2014, 07:20