growth factor activation of lam induced cell migration processes

... (1993) A 40-kDa epidermal growth factor/ transforming growth factor a-binding domain produced by limited proteolysis of the extracellular domain of the epidermal growth factor receptor J Biol Chem ... of hydrophobicity of the tyrosine at position 13 of human growth factor in receptor binding J Biol Chem 268, 10114–10119 26 Campion, S.R & Niyogi, S.K (1994) Interaction of epidermal growth factor ... model of ErbB-1, based on the structure of the type-1 insulin-like growth factor receptor [33], shows that the N-terminal part of domain II is involved in a lobe of domain I [34] Domain II of ErbB-1...

Ngày tải lên: 24/03/2014, 03:21

... and senescence Mechanisms of ERK -induced senescence Cellular senescence is an irreversible form of cell cycle arrest that prevents proliferation of damaged cells or cells that have surpassed ... evaluation of the role of insulinlike growth factors (IGF) and of type-I IGF receptor signalling in hepatocarcinogenesis and in the resistance of hepatocarcinoma cells against drug -induced apoptosis ... JAK2 dependent activation of STAT3 in addition to activation of JNK1 ⁄ and ERK1 ⁄ Cell Signal 18, 456–468 54 Matsunaga Y, Kawai Y, Kohda Y & Gemba M (2005) Involvement of activation of NADPH oxidase...

Ngày tải lên: 29/03/2014, 08:20

... participation in cell migration is highlighted by studies in T cells, whereby activation of Stat3 signaling by IL-6 is required to mediate the migration and recruitment of T cells to the site of inflammation ... required to mediate and maintain efficient cell migration 1.11 Cell migration 1.11.1 Process of cell migration Cell migration is essential to a variety of biological events and this process is ... variety of cellular responses including development, differentiation, cell growth, proliferation, immune responses and cell migration The dogma of signal transduction involves the binding of an...

Ngày tải lên: 11/09/2015, 16:06

... Chapter Table 2-1 A list of growth factors commonly used in tissue engineering* Growth Factor Epidermal growth factor Abbreviation Molecular Weight (kDa) Transforming growth factor- β Bone morphogenetic ... fibroblast growth factor Vascular endothelial growth factor 28.5 PDGF-AB 25.3 PDGF-BB Transforming growth factor- α 6.2 PDGF-AA Platelet-derived growth factor EGF 24.3 Known Activities Proliferation of ... systems Growth factors are small molecular proteins which are secreted by various types of cells Through binding to receptors on the target cells, growth factors can regulate cell migration, differentiation...

Ngày tải lên: 12/09/2015, 08:18

... for just being there ii TABLE OF CONTENTS ABSTRACT v LIST OF TABLES vi LIST OF FIGURES vii INTRODUCTION 1.1 CELLULAR ENVIRONMENT INDUCED DEATH 1.2 ANTIBIOTIC INDUCED CELL DEATH 1.3 THE COMMON DEATH ... of antibiotic action The idea of this is to manufacture more of these substances and ward off threats to human health Antibiotic induced cell death has been an area of commercial, intellectual, ... and understanding mechanisms of cell survival and cell death seems more fitting now, than ever 1.1 Cellular environment induced death Reactive oxygen species mediated cell death Aerobic organisms...

Ngày tải lên: 02/10/2015, 17:15

... intensity of the dye is proportional to the number of the viable cells Briefly, 200 lL of a suspension of PC-9 cells was seeded into each well of a 96-well plate at a density of 2000 cellsÆwell)1 ... translocation of Bax to mitochondria Some reports have shown that the activation of the Fas ⁄ FasL system may be one of the mechanisms responsible for drug -induced apoptosis in a variety of JNK activation ... between JNK activation and AG1478 -induced apoptosis, we need to study whether AG1478 induces the expression of Fas or FasL in PC-9 cells Overexpression of MKP-1 inhibited the AG147 8induced JNK activation...

Ngày tải lên: 18/02/2014, 13:20

... factors such as nerve growth factor, insulin-like growth factor, fibroblast growth factor, epidermal growth factor (EGF), and hepatocyte growth factor can suppress apoptosis of target cell populations; ... a suitable signal such as growth factors, the expression of c-Fos, one of the Fos family proteins, is induced through MAP kinase activation, allowing transactivation of genes containing AP1-binding ... Puro2 cells, but not in TAM1 (Fig 6C, lower) Finally, we evaluated the ability of EGF to rescue TMK-1 cells, Puro2 cells, and TAM1 cells from apoptosis induced by ADR EGF sup3748 pressed ADR-induced...

Ngày tải lên: 19/02/2014, 06:20

... of target genes expressed [16], and also affects the type of cellular response that is evoked [17,18] Activation of ERK is required for proliferation of fibroblasts [19] and constitutive ERK activation ... divided by the number of cells in the picture The average signal intensities of four images showed that EGF or PMA stimulated cells were comparable to untreated cells, whereas cells treated with ... from the growth factor receptor complex Thus, as the local Sos concentration at the inner surface of the membrane decreases, Ras activation is impaired as well as subsequent activation of downstream...

Ngày tải lên: 19/02/2014, 16:20

... inhibitory effect of exogenous bFGF on FSHstimulated estradiol synthesis Indeed, Sertoli cells are bFGF producing cells [2,33] and are the target of this growth factor as bFGF inhibits FSH -induced estradiol ... depending on cell type and activating growth factor [50] In NIH 3T3 ®broblasts, FiRE was shown to be selectively induced by bFGF whereas in keratinocytes, FiRE was not induced by this growth factor ... 44 Romaris, M., Bassols, A & David, G (1995) E€ect of transforming growth factor- b1 and basic ®broblast growth factor on the expression of cell surface proteoglycans in human lung ®broblasts...

Ngày tải lên: 21/02/2014, 03:20

... respectively Megakaryocytic differentiation was induced by dilution of cells at a density of 1.2 · 106 cellsÆmL)1, to a final concentration of · 105 cellsÆmL)1, with medium containing nM 12-O-tetradecanoylphorbol-13-acetate ... such translational inhibitors often belong to mRNAs encoding proteins involved in cell growth and differentiation such as growth factors, receptors, transcription factors, protooncogenes, and ... the cell- cycle of the cells was analysed by Becton Dickinson FACSort, using the Cell Quest software The Vav protein was used as a marker for differentiation To detect Vav protein level the cells...

Ngày tải lên: 22/02/2014, 07:20

... action of ATP on cancer cell growth In tumor cell lines from colon [14], endometrium [11], and esophagus [12], activation of P2Y receptors caused inhibition of proliferation by induction of cell ... activation of several signal transduction pathways The delay in cell cyle progression through activation of P2Y receptors is possibly mediated by activation of the NF-jB1 isoform of transcription factor ... receptor-mediated inhibition of cell proliferation by control of cell cycle progression has been found only in esophageal cells [12] Activation of P2Y receptors by ATP and ADP inhibits LXF-289 cell proliferation...

Ngày tải lên: 07/03/2014, 12:20

... lm [43] Studies of time dependence of cell killing by 10 lm hA indicated that cell death reached half-maximal after 24 h Figure shows that hA induced time-dependent activation of p38 kinase, which ... dose-responsive effect of hA on activation of p38 kinase In parallel experiments, we studied the effects of inhibition of ERK1 ⁄ and p38 kinase on hA -induced caspase-3 activation and apoptosis ... for human amylin -induced b -cell apoptosis (A,B) Representative western blot analysis of time-dependent activation and expression of ATF-2 Total cell extracts were prepared from CM cells untreated...

Ngày tải lên: 07/03/2014, 12:20

... muscle is the insulin like growth factor- 1 (IGF-1) ⁄ insulin-dependent pathway [5,6] Stimulation of muscle cells with growth factors such as IGF-1 leads to the activation of phosphoinositide 3-kinase ... growth factorinduced activation of Akt blocks TSC1 ⁄ TSC2 inhibition of mTOR signaling [9–11], the molecular mechanism by which Akt inhibits the function of TSC1 ⁄ TSC2 protein complex as a cell ... inhibit insulin -induced activation of mTOR signaling in C2C12 myotubes (Fig S1A) so we cannot rule out the possibility of the inhibition of other lipid or pro- Fig IGF-1 induced activation of mTOR signaling...

Ngày tải lên: 15/03/2014, 11:20

... (1993) Activation of the zymogen of hepatocyte growth factor activator by thrombin J Biol Chem 268, 22927–22932 Miyazawa K, Shimomura T & Kitamura N (1996) Activation of hepatocyte growth factor ... of HGF via HGFA, which leads to endothelial cell growth ⁄ migration and contributes to repair of the blood vessel structure HGF also stimulates the proliferation and migration of epithelial cells ... Naka D, Morimoto Y & Kitamura N (1995) Activation of hepatocyte growth factor by two homologous proteases, blood-coagulation factor XIIa and hepatocyte growth factor activator Eur J Biochem 229,...

Ngày tải lên: 15/03/2014, 11:20

... stimulation of PC12 cells results in activation of the Smad pathway independently of TGF-b1 This activation is direct and results in nuclear translocation of Smad3 within only 30 of NGF treatment ... kinases Cellular responses induced by bone morphogenetic protein (BMP) for example, can be impaired by epidermal growth factor and hepatocyte growth factor, which lead to the phosphorylation of Smad1 ... phosphorylation of the C-terminal SSXS-motif of the R-Smad Based on the diverse mechanism of Smad activation by either TGF-b1 or NGF, specific subsets of target genes might be induced The potential of NGF...

Ngày tải lên: 16/03/2014, 16:20

... and activation of p38 of the SAPK2 subfamily from the sponge Suberites domuncula Biol Cell 29, 95–104 29 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the head of ... Preparation of water-soluble and water-insoluble poly(acrylamide-allylamine) derivatives of polysaccharides Int J Bio-Chromatogr 2, 37–47 27 Castro, R., Couso, N., Obach, A & Lamas, J (1999) Effect of ... series of experiments it can be concluded that curdlan initiates an activation circuit, from the surface Ó FEBS 2004 Activation of sponge cells by (1fi3)-b-D-glucan (Eur J Biochem 271) 1935 of the...

Ngày tải lên: 16/03/2014, 16:20

... transforming growth factor- b1 (TGF-b1) of the proinflammatory cytokine TNF-a via the induction of FXR1 is the focus of this article TGF-b1, a member of the large transforming growth factor- b superfamily ... RAW 264.7 cells treated with LPS (100 ngÆmL)1) for h, the level of TNF-a protein induced showed a decrease (Fig 1A,B) The response of the BMDMu was comparable to that of the RAW 64.7 cells Assessment ... mL tube: 20 lL of Optimem, lg of each plasmid, and lL of Lipofectamine LTX (Invitrogen) and incubated at room temperature for 30 This mixture was then added slowly to cells in mL of Optimem The...

Ngày tải lên: 22/03/2014, 21:21

... indicated time points factor- binding proteins would increase the pericellular concentration of insulin-like growth factor, and this could eventually stimulate invasive growth of tumor cells [21,40] Our ... analysis of the cDNA for a human serine protease responsible for activation of hepatocyte growth factor J Biol Chem 268, 10024–10028 Lee SL, Dickson RB & Lin CY (2000) Activation of hepatocyte growth ... Activation of the zymogen of hepatocyte growth factor activator by thrombin J Biol Chem 268, 22927–22932 11 Miyazawa K, Shimomura T & Kitamura N (1996) Activation of hepatocyte growth factor in...

Ngày tải lên: 23/03/2014, 07:20

... Regulated activation of MIG-17 ⁄ ADAMTS S Ihara and K Nishiwaki staining of cross-sections of L3 larvae with antibody to GFP revealed signals in the cytoplasm of muscle cells, the source of MIG-17 ... including both the proform and the activated form, was immunoblotted (right lane) Single asterisk, proform of MIG17–GFP; double asterisk, activated form of MIG-17–GFP; arrow, proform of MIG-17– 6His; ... proform localizes to the surfaces of the gonad, intestine and hypodermis corresponding to the basement membranes [17] To determine the sites of activation of MIG-17 and tissue distribution of...

Ngày tải lên: 30/03/2014, 04:20

... showing the roles of PAK activation on LPA -induced FAK phosphorylation and cell motility Therefore, we undertook to investigate the roles of PAK1 activation in LPA -induced cell motility and present ... mutants of each of Rac1, Cdc42 and RhoA to assess the involvement of Rho family members in LPA -induced PAK1 activation N17Rac1- or N17Cdc42-transfected cells showed reduced activation of PAK1 ... motility of PAK1 K299R-transfected cells in the presence of LPA was markedly slower than that of vector transfectant, indicating that the activation of PAK1 is necessary for LPA -induced A2058 cells...

Ngày tải lên: 30/03/2014, 13:20