growth arrest differentiation and apoptosis

... accelerates muscle and motor neuron regeneration FASEB J 17, 53–55 Rosenthal SM & Cheng ZQ (1995) Opposing early and late effects of insulin-like growth factor I on differentiation and the cell cycle ... (1996) Growth hormone and the insulin-like growth factor system in myogenesis Endocrine Rev 17, 481–517 DeVol DL, Rotwein P, Sadow JL, Novakofski J & Bechtel PJ (1990) Activation of insulin-like growth ... primarily during embryogenesis and regeneration [28,29], IGF-1 has been reported to be essential for muscle differentiation and hypertrophy [1–3] Unlike other growth factors, IGF-1 is able to...

Ngày tải lên: 19/02/2014, 06:20

... and AgNOR References Cell growth and cytotoxicity assays Philip Skehan Introduction Growth and cytotoxicity assays Quantifying cell growth Experimental design Growth rate and doubling time Potency ... cytometric differentiation between G2 and M and between post-mitotic and pre-synthetic G1 (from ref 5) Equipment and reagents • • • BrdU PBS RNase A 0.1 M HCI PBST: ml ice-cold PBS and 0.4% Tween-20 ... Cell growth and kinetics in multicell spheroids 61 Ralph E Durand Introduction 61 Growth of spheroids Options Example: the V79 spheroid system 61 61 64 Special features of spheroids Metabolite and...

Ngày tải lên: 10/04/2014, 11:13

... cycle arrest and cell apoptosis by 3b protein expression Induction of cell cycle arrest and cell apoptosis by 3b protein expression p3b/EGFP-N1 plasmid was transfected into COS-7 cells, and the ... positive cells negative cells Figure Figure Histogram of cell cycle arrest and cell apoptosis Histogram of cell cycle arrest and cell apoptosis Histogram showing the percentages of cells at various ... percentages in apoptosis and necrosis were both lower and comparable in the positive and negative pEGFP-N1 transfected cells (Figure 3b) However, for p3b/EGFP-N1 transfected cells, the apoptosis cells...

Ngày tải lên: 19/06/2014, 08:20

... cycle arrest and cell apoptosis by 3b protein expression Induction of cell cycle arrest and cell apoptosis by 3b protein expression p3b/EGFP-N1 plasmid was transfected into COS-7 cells, and the ... positive cells negative cells Figure Figure Histogram of cell cycle arrest and cell apoptosis Histogram of cell cycle arrest and cell apoptosis Histogram showing the percentages of cells at various ... percentages in apoptosis and necrosis were both lower and comparable in the positive and negative pEGFP-N1 transfected cells (Figure 3b) However, for p3b/EGFP-N1 transfected cells, the apoptosis cells...

Ngày tải lên: 20/06/2014, 04:20

... (Figure 3) and camptothecin and cis-platin used as standards (Figure 4) Apoptosis recorded in untreated control MOLT-4 and HL-60 cells were 3.61% and 2.54% respectively In MOLT-4, total apoptosis ... number Analysis of apoptosis in MOLT-4 and HL-60 cells by Annexin V-FITC/PI double staining method MOLT-4 and HL-60 control and treated cells were stained with annexin V-FITC/PI and gated into LR ... rise in sub-G1 fraction, light and electron microscopic studies showing morphological imprints of apoptosis and marked increase in caspase and in treated cells Apoptosis is controlled by a diverse...

Ngày tải lên: 10/08/2014, 10:20

... to better understand the molecular basis of Osthole induced G2/M arrest and apoptosis, we investigated the expression of p-Akt and t-Akt after treatment with Osthole(0, 50, 100, and 150 μM) for ... cycle arrest and apoptosis are common mechanisms proposed for the cytotoxic effects of anticancer-drug extracted from herbal medicine [23] Cell cycle arrest can trigger proliferation inhibition and ... mechanisms through which Osthole induces apoptosis The PI3K/Akt is one of the most important signaling pathways in regulating cell growth, proliferation and apoptosis, and Akt is a major downstream target...

Ngày tải lên: 10/08/2014, 10:21

Ngày tải lên: 13/08/2014, 03:20

... patient demographic data and diagnoses have been presented elsewhere [16] Pneumonia and urinary tract infections were common and also overrepresented in the severe sepsis and the sepsis groups Infected ... cell- and tissue-types may be the source of the sAxl in sepsis Both Axl and Mer have been shown to be shed under the influence of PMA and LPS [10,19] Gas6 correlates with IL-6, procalcitonin and ... Gas6, procalcitonin and number of failing organs, indicating that both Gas6 and sAxl are increased in inflammatory states Gas6 and sAxl are increased during organ failure, and Gas6 is increased...

Ngày tải lên: 13/08/2014, 21:21

... dopaminergic neuronal differentiation through initiating neurite outgrowth and inducing growth arrest The findings and proposed future work may eventually contribute to the understanding of pathways ... neuron differentiation 1.8 Literature reviews on factors that cause neurite outgrowth and differentiation in MN9D At present, there are only papers documented on investigating neurite outgrowth and ... other cellular functions, such as cell growth and differentiation, embryonic development, tumor progression, metastasis, and apoptosis (Otero 2000; Hartsough and Steeg 2000; Kimura et al 2000; Lacombe...

Ngày tải lên: 08/11/2015, 17:09

... and vaguely lobulated Well developed fronds consist of epithelial-lined stromal projections that protrude into dilated glands or cystic spaces The glands are usually widely spaced, dilated, and ... pleomorphism, stromal overgrowth, tumor necrosis and heterologous stromal elements, or a combination of histological features to be prognostically useful On the other hand, several authors have ... that include the degree of stromal hypercellularity, stromal cytologic atypia and mitotic activity, stromal overgrowth, and circumscribed vs invasive margins [2] Although histological features have...

Ngày tải lên: 31/10/2012, 16:57

... muscles, or glands, survive Other brain cells die Sometimes just parts of cells die off For instance, many more synapses are formed than needed between neurons and other neurons, muscles and glands Those ... muscles and glands Those synapses that are used remain and get stronger, the other synapses detach, and the axons shrivel and die Which brain cells and which of their synapses survive depends on their ... child The details of embryonic development and mechanisms of cell differentiation differ between plants, fungi, protists, and various invertebrate and vertebrate animals We will look at some...

Ngày tải lên: 26/12/2013, 01:18

... production: capital, people and land - and land is always far cheaper outside cities than inside Why don't capital and people move outside, combining themselves with cheaper land and increasing profits?" ... contributions of expanding (and improving) labor supplies, and increased capital investment to driving growth These efforts at growth accounting” showed that most of the growth of the economy ... practices, cultural attitudes and values, and reputation, and the social constructs that guide and enable interpersonal and business relations History influences the pace and trajectory of knowledge...

Ngày tải lên: 24/01/2014, 00:20

... (longterm) capital and ultimately spur firm growth These results hold for different model and hausbank-proxy specifications and are in line with Petersen and Rajan (1994) and Berger and Udell (1995) ... are of importance to economic growth as shown by Lucchetti, Papi, and Zazzaro (2001) and Koetter and Wedow (2006) Hence, we include in Z regional macroeconomic and banking market covariates, ... predicted growth rates IGR and SGR allowing for faster growth without the dependence on external finance 15 Results The regressions in Table and investigate whether firms which exceed their internally and...

Ngày tải lên: 16/02/2014, 12:20

... resistance to apoptosis Apoptosis is a tightly regulated mechanism for the disposal of damaged cells and to remove cells during normal growth and development [27,40] Cells that undergo apoptosis ... tumorigenesis and metastasis [12,13], and the tyrosine phosphatase SHP-2 is necessary for activation of ERK in response to a number of growth factors, including insulin growth factor-1, platelet-derived growth ... with endosomal and Golgi structures using specific markers (EEA1, TfnR for endosomes and GM130, TGN46 for Golgi) and found that JSP1-wt partially colocalized with the Golgi apparatus and showed minimal...

Ngày tải lên: 16/02/2014, 14:20

... lane 6, Glu-Pg- and mAb 34D3-treated cells Bands in lanes and correspond to forms I and II of plasmin(ogen) High molecular weight bands in lanes and correspond to mAb A10.2- and mAb 34D3-plasminogen ... Lys-plasmin(ogen) binding and activation These structural–functional transitions are determinant in the initiation and development of both fibrinolysis and cell detachment-induced apoptosis and may therefore ... their respective contribution to Gluand Lys-Pg binding and activation onto fibrin and cells, as well as their effects on fibrinolysis and on plasmin-induced cell apoptosis The LBS specificity of these...

Ngày tải lên: 20/02/2014, 01:20

... 2B that VBARP-L and VBARP-S are coded by these specific exons VBARP-L and VBARP-S code for precursor proteins of 627 and 435 amino acids and with calculated peptide masses of 69 and 49 kDa, respectively ... streptomycin and interleukin-2 growth factor Monocytes were isolated using CD14 beads and differentiated into macrophages and dendritic cells using GM-CSF (500 UÆmL)1) ⁄ M-CSF (15 ngÆmL)1) and GM-CSF ... were mixed and immunoprecipitated with VBARP-, Vpr- or Nef-specific antibody and analyzed by autoradiography (Fig 1A) Results indicate that both Vpr and VBARP were able to form a complex and the...

Ngày tải lên: 07/03/2014, 21:20

... 22d and 22g; credit to the government: lines 22a, 22b and 22c; interest rates: lines 60b,c,l,p and 61; CPI and PPI: lines 64 and 63; nominal GDP: lines 99b and 99b.c; real GDP: lines 99bvp and ... in Estonia, Latvia 13 and Croatia and also perhaps in Hungary and Slovenia On the other hand, initial undershooting might not be a serious problem for Lithuania, Poland and Romania because the ... Ariccia and I Vladkova-Hollar (2005): Early birds, late risers and sleeping beauties: Bank credit growth to the private sector in Central and Eastern Europe and in the Balkans, Journal of Banking and...

Ngày tải lên: 15/03/2014, 04:20

... activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation of caspases-9 and -3 were analyzed Cells over-expressing HSP70 and its deletion ... stressinduced apoptosis pathway [17] It prevents caspase-3 and stress-activated protein kinase ⁄ Jun kinase (JNK) activation [18] and mitochondrial depolarization [19], blocks apoptosome formation and ... of the release of Smac and apoptosis GAPDH Ratio of HSP70 to GAPDH 14 # 12 # 10 * The role of the ATP-binding domain of HSP70 in the prevention of the release of Smac and apoptosis after exposure...

Ngày tải lên: 16/03/2014, 01:20

... (TNF) receptorassociated death domain and of the TNF-related apoptosis- inducing ligand, leading to the activation of death-receptor signaling and caspase-mediated apoptosis [59] Activated Akt phosphorylates ... to control growth and trigger apoptosis in human cancer cells by targeting heterotrimeric G proteins and individual GPCRs [79,80] However, experimental data obtained from transgenic and knockout ... [2,42], and the overexpression or mutation of many GPCR subtypes in numerous cell types is thought to contribute to deregulated growth and tumour development [43,44] The transmembrane and intracellular...

Ngày tải lên: 16/03/2014, 05:20

... cAMP can enhance osteoclast differentiation by receptor activator of NF-jB ligand (RANKL) [16] and neuronal differentiation by nerve growth factor (NGF) or epidermal growth factor (EGF) [17] Although ... receptor-mediated differentiation Mol Cell Biol Res Commun 1, 144–152 Hamilton JA (1983) Glucocorticoids and prostaglandins inhibit the induction of macrophage DNA synthesis by macrophage growth factor and ... transactivation of the receptors for epidermal growth factor and nerve growth factor, thereby modulating activation of MAP kinase, Akt, and neurite outgrowth in PC12 cells J Biol Chem 277, 43623–43630...

Ngày tải lên: 16/03/2014, 22:20