gfr estimation with an internal quality control

... Menzel-Gläser, Braunschweig, Germany), deparaffinized and stained with Alcian blue/van Gieson or immunohistochemically with anti-type I collagen and antitype II collagen antibodies Immunohistochemistry ... endogenous control, 18S rRNA labelled with VIC/TAMRA was used (Applied Biosystems) Reverse transcription in vitro was performed with 500 ng of total RNA with the use of random hexamer primers and TaqMan ... affected and unaffected sides, without any correlation with Mankin score In the immunohistochemical analysis for type I collagen, both samples from one donor (a male aged 74 years) with Mankin scores...

Ngày tải lên: 09/08/2014, 06:22

... trypan blue 2.1.3 Statistical Analysis Results are expressed as mean ± SD Significance between mean values was determined by Student’s t-test Samples were analyzed by two-sample equal variance, and ... assay Transfection efficiency C 2100% 1800% 1500% 1200% 900% 600% 300% 0% untransF EGFP-SPHK1transF SPHK2-transF Figure 2.4 Transfection efficiency of SPHK1 and SPHK2 CHO cells transfected with ... tested (10μM, 25μM, 50μM, 75μM, and 100μM), none of the six compounds inhibited DAGK and human PKC alpha (Figure 2.8 and Figure 2.10) On the other hand, and in agreement with previous findings (Kim...

Ngày tải lên: 11/09/2015, 16:06

... probed with primary and secondary antibodies The primary antibodies against bone matrix proteins used were: rabbit anti-human osteocalcin (Chemicon, #AB1858) (1:500 dilution); rabbit anti-human osteopontin ... significant 3.2 RESULTS 3.2.1 RNA Expression of SPHK1 and SPHK2 in Human BM- and ADMSCs Human BM-MSCs and AD-MSCs were tested on their mRNA expression of SPHK1 and SPHK2 using real time PCR, and ... are expressed as mean ± SD Significance between mean values was determined by Student’s t-test Samples were analyzed by two-sample equal variance, and twotailed distribution, with a value of P

Ngày tải lên: 11/09/2015, 16:06

... antibody (Santa Cruz, #8035); secondary antibody: anti-mouse IgG HRP (Sigma, A4416)) was used for equal loading control 4.1.6 Statistical Analysis Results are expressed as mean ± SD Significance ... in human BM- and AD- MSCs proliferation Figure 4.6 Function of S1P and serum in human BM- and AD- MSCs proliferation Human BM-and AD- MSCs were cultured in different conditions for days, and cell ... expression in human BM- and AD- MSCs Table B and C represent data from two donors cells of AD-MSCs Mouse IgG1 and IgG2a were used as the isotype controls and five thousand events per antibody group...

Ngày tải lên: 11/09/2015, 16:06

... in combination with PDGF-AB, or reduced serum, can be an important element in the development of better defined serum-free or serum-reduced strategies to promote human-adult BM- and AD- MSCs proliferation ... develop a new strategy controlling human MSCs osteogenic and adipogenic differentiation switching To our knowledge, this is the first report on inhibitors of SPHK mediating human BMand AD- MSCs differentiation ... more controlled and facilitate differentiation of stem cells, for future 154 Chapter Conclusion and Future Work therapeutic applications to balance the abnormal portions of osteoblasts and adipocytes...

Ngày tải lên: 11/09/2015, 16:06

... two psoriasis susceptibility loci (HLA and 17q) and two novel candidate regions (16q and 20p) by genome-wide scan." Hum Mol Genet 6(8): 1349-56 Nirmalanandhan, V S., M Rao, et al (2007) "Effect ... E., L De Angelis, et al (2004) "Isolation and expansion of adult cardiac stem cells from human and murine heart." Circ Res 95(9): 911-21 Mets, T and G Verdonk (1981) "In vitro aging of human bone ... Khovidhunkit, Weerapan, MinSun Kim, et al (2004) “Effects of Infection and Inflammation on Lipid and Lipoprotein Metabolism: Mechanisms and Consequences to the Host” J Lipid Res 45(7):1169-96 Kilian, O.,...

Ngày tải lên: 11/09/2015, 16:06

... proliferation and differentiation 1.3 SPHK AND S1P REGULATION IN STEM CELL RESEARCH As was mentioned above, S1P can act as an intracellular second messenger and as an extracellular ligand for specific ... to suppress inflammation and as potential anti-cancer therapies Another major aim is to study the roles of SPHK and S1P in stem cell proliferation, stemness maintenance and differentiation In particular, ... human adult stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human adult stem cell proliferation and differentiation More details about the research goals and...

Ngày tải lên: 11/09/2015, 16:06

... enhanced transcription of ribosomal RNA and tRNA genes and to stimulate transcription of many genes involved in all steps of protein translation (Arabi et al 2005; Boon et al 2001; GomezRoman et al ... (Miliani de Marval et al 2004), and fibroblasts lacking D-type cyclins are resistant to transformation by Myc (Kozar et al 2004), while fibroblasts expressing any single D-type cyclin can be transformed ... correlated with expression of specific MYC genes in human tumors, arguing that Myc proteins act upstream of E2F-dependent transcription in human tumorigenesis (Hernando et al 2004) [B Samans and M...

Ngày tải lên: 25/10/2013, 21:20

... accumulates and translocates into the nucleus, forms a complex with T cell factor ⁄ lymphocyte enhancer factor-1 (TCF ⁄ LEF-1) family transcription factors, and regulates Wnt target genes and proliferation ... fibroblasts HFL1 were transfected with siRNAs targeted to b-catenin and luciferase (control sequence) After 72 h, the cells were incubated with or without a-defensin-1 (B: 1.25–2.5 lM) and a-defensin-2 ... statistically significant Acknowledgements This work was supported by NIH grant R01HL088261, Flight Attendant Medical Research Institute grants 032040 and 072104, and American Heart Association...

Ngày tải lên: 18/02/2014, 06:20

... (between and 30 µM) in culture medium, and with or without anti-α6 IgG (0.5 µg/ml) (a, b): percentages of round cells at 48 h of culture; *** : p < 0.001, with anti-α6 IgG vs control and PD98059-treated ... design and coordination, statistical analysis and interpretation of data and drafted the manuscript FLB, SF and DM read and approved the final manuscript CP died during preparation of the manuscript ... Luu-The (Quebec, Canada) and rabbit polyclonal antibody raised against ERK1 and ERK2 (anti-ERK1/2) was purchased from Santa Cruz (Le Perray-en-Yveline, France) Rabbit polyclonal antibody raised...

Ngày tải lên: 05/03/2014, 17:20

... ˜ DHEA-induced antiproliferative effect in MCF-7 cells is androgen- and estrogen receptor independent Cancer J 12, 160–165 Liu D, Iruthayanathan M, Homan LL, Wang Y, Yang L, Wang Y & Dillon JS ... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsible for converting androgen ... benz(a)anthracene-induced skin papillomas and carcinomas by dehydroepiandrosterone and 3-b-methylandrost-5-en-17-one in mice Cancer Res 45, 164–166 Schwartz A & Pashko L (1995) Cancer prevention with...

Ngày tải lên: 16/03/2014, 00:20

... 11–17 Granata R, Settanni F, Biancone L, Trovato L, Nano R, Bertuzzi F, Destefanis S, Annunziata M, Martinetti M, Catapano F et al (2007) Acylated and unacylated ghrelin promote proliferation and ... Radhakrishnan R & Dhanasekaran DN (2006) Transactivation of platelet-derived growth factor receptor a by the GTPase-deficient activated mutant of Ga12 Mol Cell Biol 26, 50–62 31 Slomiany BL & Slomiany ... applicability of such an approach for therapeutic benefit is in its infancy but, as we have described, progress has been made with efforts to control growth and trigger apoptosis in human cancer cells by...

Ngày tải lên: 16/03/2014, 05:20

... were analyzed by electrophoresis in 1.7% agarose gels and visualized with SYBR Green I Data measurement and statistical analysis Radiotracer, cell number and enzyme assay data were quantified and ... mono-ADP-ribosyltransferase by angiotensin II and prostaglandin E2 Quiescent SMC were treated with AngII (1 lM) or PGE2 (1 lM) over 120 and subcellular fractions prepared as described in Materials and methods ... 48 h in a standard CO2 incubator at 37 °C, the membrane was removed from the chamber and placed into methanol for Cells were scraped from the upper side of the membrane and the membrane was subsequently...

Ngày tải lên: 17/03/2014, 09:20

... greater than those obtained with GFP transfection (Table 2) However, cells transfected with DP8–V5–His or DP9–V5–His showed no significant differences from those transfected with vector only Transfection ... This is an assay of cell migration rather than proliferation [19] Cells transfected with DP8–GFP and those transfected with DP9–GFP exhibited reduced migration into wounds on collagen-coated and ... DP9–GFP-transfected (E, F) cells compared to untransfected control 293T cells These analyses show data from all live cells To demonstrate that the method could detect DPIV and FAP, DPIV-transfected and...

Ngày tải lên: 23/03/2014, 11:20

... proteins (20 lg per lane) were subjected to SDS ⁄ PAGE and western blotting using specific antibodies against p130Cas (A and B), PKCd (C and D, upper panel) and actin (C and D, lower panel) as described ... as the mean ± standard error of the mean Results were analyzed by one-way analysis of variance (anova) followed by Student’s t-test P-values of less than 0.05 were considered significant Acknowledgements ... PAGE and analyzed by western Blotting using anti-PKCd (upper panel) and anti-actin (lower panel) as described Molecular mass (kDa) is indicated by lines on the left Full-length PKCd and a cleavage...

Ngày tải lên: 23/03/2014, 11:20

... overnight, dewaxed and rehydrated Both tissue sections and the cell culture samples described above were incubated with anti-PP17 antibody, and with monoclonal anti-bax and anti-bcl2 antibodies for ... H, cells were stained with PP17, in C and D with anti-bax, in E and F with anti-Bcl2 Ig Compared with controls, after paclitaxel treatment, synthesis of PP17 variants (B) and bax (D) proteins ... fraction were quantified with densitometric scanning and shown semiquantitavely (C) In total milk (lane 1), high amounts of PP17b and PP17c were found, while in MLMG fraction (lane 2), mainly PP17b...

Ngày tải lên: 23/03/2014, 21:20

... measures either an advancing angle or a receding angle, and the hysteresis between them is significant Both refer to metastable states [21] It is common practice to use the static advanced angle to ... requirements of ANSUNISO Printed in The Netherlands Preface Volume I of Adhesion Science and Engineering dealt with the mechanics of adhesive bonds and the rheology of adhesives Volume I1 deals with the ... with the top line corresponding to the advancing angle, and the lower line corresponding to the receding angle does not contact the solid The effective contact angle over such gaps is 180", and...

Ngày tải lên: 26/04/2014, 15:36

... Abcam), mouse anti-E-cadherin (1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-bcatenin (1:500; Santa Cruz), mouse anti-vimentin (1:500; Santa Cruz), mouse anti-C-myc (1:200; Santa Cruz), ... Diallo OT, Hu M, Ehsanian R, Yang X, Arun P, Lu H, Korman V, Unger G, Ahmed K, et al: CK2 modulation of NF-kappaB, TP53, and the malignant phenotype in head and neck cancer by anti-CK2 oligonucleotides ... membrane were fixed with methanol, stained with hematoxylin, and counted under a microscope in five randomly selected fields at × 200 magnifications Statistical analysis All statistical analyses...

Ngày tải lên: 18/06/2014, 19:20

... assay and study about the genotoxicity effect were done by ANOVA test Figure The correlation between measured absorbance (mean ± SD) and the various borax concentrations by MTT assay Mean absorbance ... concentration of 0.1, 0.15, 0.2, 0.3 and 0.6 mg/ml for 24 hours The value of mean absorbance reflected the anti-proliferation of lymphocyte by borax Mean absorbance conversion can be directly related to ... comparing with the control group Figure showed the sister chromatid exchange occurrence in human chromosomes of the control group Figures 6A, B showed the sister chromatid exchange occurrence in human...

Ngày tải lên: 20/06/2014, 00:20