genomic characterization of the inherited bone marrow failure syndromes

Báo cáo y học: "Genomic characterization of the Yersinia genus" doc

Báo cáo y học: "Genomic characterization of the Yersinia genus" doc

... necessarily reflect the official policy or position of the US Department of the Navy, US Department of Defense, or the US Government Some of the authors are employees of the US Government, ... covered repeat regions and therefore had significantly increased copy number, the quality of the sequence of the draft assem- blies was high, with less than 0.1% of the sequence of each genome having ... analyzed the contribution of the genome to reducing the size of the Yersinia core protein families set. If Y. ruckeri was excluded, the Yersinia core would be 2,232 protein families of N = 2 rather

Ngày tải lên: 09/08/2014, 20:21

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Báo cáo sinh học: " A specific pattern of splicing for the horse αS1-Casein mRNA and partial genomic characterization of the relevant locus" pot

Báo cáo sinh học: " A specific pattern of splicing for the horse αS1-Casein mRNA and partial genomic characterization of the relevant locus" pot

... contain the β-Cn gene and one (577-C-4) of them included both the αS1-Cn and the β-Cn genes. The size of the insert was estimated by FIGE to be ca. 82-kb. Given the close proximity of the caprine, ... analysed the genomic organisation of a part of the casein locus which presents a large similarity with the loci of the other species described so far. This knowledge provides tools to study the regulation ... Lastly, the size of the β-Cn locus was determined by long-range PCR using the primers Eqβ3 (matching with exon 2) and Eqβ2 (matching with the 3  extremity of the gene). Gel electrophoresis of the

Ngày tải lên: 14/08/2014, 13:21

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Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair

Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair

... matrices and their integrin partners on the cell fate and development of CLCs vs, MSCs in vitro and in vivo and To compare the relative therapeutic efficacies of the cell types Adult human bone marrow ... CHARACTERIZATION OF ADULT HUMAN BONE MARROW MESENCHYMAL STEM CELLS FOR EFFECTIVE MYOCARDIAL REPAIR GENEVIEVE TAN MEI YUN (B.Sc (Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... levels of troponin T, sarcomeric -actin and RyR2 in CLCs cultured on collagen V ECM Importantly, the engraftment of CLCs within close proximity of collagen V-expressing myofibers promoted their

Ngày tải lên: 12/09/2015, 09:42

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Báo cáo y học: "Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" pptx

Báo cáo y học: "Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" pptx

... on cloning of the entire genome in BAC and trans- fecting the BAC-contained viral cDNA under the control of a CMV promoter [37]. The use of BAC DNA provides the remarkable benefit of being able ... molecu- lar weight than those from the cell culture isolate, indicat- ing absence of the deletion in the patient. Of note, clinical samples from the wife of the index patient, who got infected ... such as the SARS-CoV can persist in their reservoirs without causing disease. The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own. The study

Ngày tải lên: 12/08/2014, 04:20

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Báo cáo khoa học: " Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" docx

Báo cáo khoa học: " Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" docx

... on cloning of the entire genome in BAC and trans- fecting the BAC-contained viral cDNA under the control of a CMV promoter [37]. The use of BAC DNA provides the remarkable benefit of being able ... molecu- lar weight than those from the cell culture isolate, indicat- ing absence of the deletion in the patient. Of note, clinical samples from the wife of the index patient, who got infected ... such as the SARS-CoV can persist in their reservoirs without causing disease. The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own. The study

Ngày tải lên: 12/08/2014, 04:20

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Báo cáo y học: "Natural history and clinical significance of MRI-detected bone marrow lesions at the knee: a prospective study in community dwelling older adults" pot

Báo cáo y học: "Natural history and clinical significance of MRI-detected bone marrow lesions at the knee: a prospective study in community dwelling older adults" pot

... There are conflicting data on the natural history and clinical significance of bone marrow lesions (BMLs) The aims of this study were to describe the natural history of MRI-detected BMLs at the ... consisted of several abnormalities including bone marrow necrosis, abnormal trabeculae, bone marrow fibrosis, bone marrow bleeding, and bone marrow oedema [8] BMLs have also been described in other ... transient osteoporosis of the hip [12] and are often referred to as bone marrow oedema (BME) In RA, it is suggested that BME represents cellular infiltrate within the subchondral bone [9] and is associated

Ngày tải lên: 12/08/2014, 15:22

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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells

Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells

... the inner part of the membranes As shown in Figure 4e, many big aggregates appear in the middle part of the PVDF-PS membrane The hydrophobic property of PS polymer promoted the formation of these ... Thermal analysis of the PVDF-SPS composite membrane The TG analysis curve is presented in Figure for the investigation of the thermal stability of the PVDF membrane, PVDF-PS membrane, and the PVDF-SPS ... decomposes The swelling is due to the degradation of the cross-linking structure of the membrane in the H2O2 solution [33] The decomposition rate is larger than the swelling effect after 60 h The result

Ngày tải lên: 05/09/2013, 16:11

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Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx

Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx

... hydrate VI hydrate thermal eventa loss of water melt of Form III? loss of water melt of Form III Form I to Form II melt of Form II loss of water melt of Form III Form I to Form II melt of Form II Form ... Form I to Form II melt of Form II melt of Form VI melt of Form VI Form I to Form II melt of Form II loss of water melt of Form VI loss of water melt of Form VI Table Summary of combined DSC/XRD ... anhydrous thermal event Form I to Form II melt of Form II Form I to Form II melt of Form II loss of water melt of Form I Form I to Form II melt of Form II loss of water melt of Form III melt of Form

Ngày tải lên: 14/02/2014, 03:20

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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

... unclear whether these cytokines are produced by CD4+CD25+Foxp3+Treg or whether they induce the production of these cytokines from another population of cells [20] To date, our knowledge about the different ... important in the generation of the correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and other extracellular parasites [2] Th17 is the most ... protein domains important in the functioning of these transcription factors The gene organizations of zebrafish t-bet and foxp3 were identical to those of the human genes, with the second foxp3 transcript

Ngày tải lên: 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

... to the GBS of the ribozyme for the exon-ligation step of TES. In the substrate-cleavage step, however, the 3¢ terminal guanosine (G336) of the ribozyme (Fig. 1) is binding to that same GBS. Therefore, ... that there is room for improve- ment in terms of the rate of reaction. This would be beneficial not so much in terms of the rate of the over- all reaction, as the cleavage reaction is not the limiting step ... group are required for the second step of TES [48], similar to (if not the same as) the role of the xG in the second step of self-splicing [48–56]. This suggests that the guanosine to be excised

Ngày tải lên: 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro- teins. The ... to model the entire domain without it. Another significant finding concerns the nature of the acid residues within the active site of Rep245. The carboxylate triad of Rep245 including the D101, ... reveal that the novel fold in the N-terminal mod- ules of the catalytic cores of AEPs and prim–pols is unrelated to that of other known polymerases, whereas the RRM-like fold encompassed by their C-terminal units

Ngày tải lên: 18/02/2014, 18:20

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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx

Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx

... required for the protonation of the amine groups of samples. The number of equivalents of acid groups was calculated using the formula: [NH 3 + ](mequiÆkg )1 ) ¼ 1000 · molarity (moles/L) · volume of titrant ... (mL)/mass of sample (g) and the level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight of the sample) and the equivalent weight of GlcN [23]. Circular ... constitutes the exoskeleton of inverte- brates and is one of the components of the cell walls of fungi. It has wide and varied applications in medicine, agriculture, pharmaceuticals and the food

Ngày tải lên: 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... suggest- ing that the origin of these forms dates back to before the occurrence of vertebrates, i.e. more than 500 mya. This dates the differentiation of the MAPEG forms back to the late Cambrian ... for which the novel pattern is located in the third loop. The reason for the similarity and location of these pat- terns could be a result of short evolutionary time rather than gain of new features ... analysis of the predominant band displaying the correct molecular mass, purified from the gel, yielded the expected sequence. The partially purified protein constitutes a major part of the preparation

Ngày tải lên: 19/02/2014, 17:20

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Đề tài " Numerical characterization of the K¨ahler cone of a compact K¨ahler manifold " doc

Đề tài " Numerical characterization of the K¨ahler cone of a compact K¨ahler manifold " doc

... ω p−1 ≥ 0. NUMERICAL CHARACTERIZATION OF THE K ¨ AHLER CONE 1249 In other words, the dual of the nef cone K is the closed convex cone generated by cohomology classes of currents of the form [Y ] ∧ ... characterization of the dual cone of the nef cone, in the space of real cohomology classes of type (n − 1, n − 1) This leads immediately to Corollary 0.4 In the case of ... the K¨hler cones Kt of the various a −1 (t), as t runs over S Because of the assumption of local fibres Xt = π triviality of π, the topology of Xt is locally constant, and therefore

Ngày tải lên: 05/03/2014, 23:20

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Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt

Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt

... inactivation of the enzyme. Thus, the results obtained in the pres- ent study extend our knowledge of the nitrogenase post-translational modification system outside of the a-class to other members of the ... electrophoresis. Another striking difference of the MALDI-TOF spec- tra from the modified Fe-protein in comparison to the unmodified Fe-protein is the decreased intensity of peak 1625.4 m ⁄ z and the absence of ... under these conditions. The strong effect of TFA on the arginine- specific ADP-ribosylation might be caused by the high degree of acidity of this acid with its pK a value of 0.26 compared to the other

Ngày tải lên: 07/03/2014, 02:20

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Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

... bond The occurrence of the C17 dialdehyde confirmed the novel site at the C7¢–C8¢ double bond observed with apolycopenals and implied the formation of geranial Accordingly, the GC-MS analyses of the ... MeOH solution of the HPLC-purified compound, and then incubating for 20 at 50 °C The product was then partitioned against petroleum ether ⁄ diethyl ether : (v ⁄ v) The identification of the oxime was ... depends on the nature of the substrate The cleavage of a sole double bond in the cyclic moiety of 3-OH-c-carotene and of monocyclic b-apocarotenals with different chain lengths indicates that the b-ionone

Ngày tải lên: 07/03/2014, 03:20

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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... organs of members of the small plant family Caricaceae [1]. The two economically most important genera of this family are the commonly grown tropical species Carica papaya and the group of highland ... higher concentration of enzymes in the latex of Vas- concellea fruits, but in addition also results from the presence of other cysteine proteinases or isoforms. In contrast to the cysteine proteinases ... proteinases or isoforms in the latex. To investigate these two hypotheses, the protein concentration per milligram of dried latex, as well as the amidase activity per milligram of protein, was measured

Ngày tải lên: 07/03/2014, 11:20

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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... similar pattern of five bands, one of which was also more intensive than the others. Thus, the different intensity of the bands may be the result of a different copy number of the genes in the hybridizing ... otherwise, the efficiency of the amplification will be lower than that of the considered variants, and this will result in an overestimation of the amount of genomic DNA containing one copy of the ... quantitative ratio of the amplification products of the three gene variants in the amplified fragment was assessed by calculation of the ratio of radioactivity of the corresponding bands. Quantitative PCR The

Ngày tải lên: 07/03/2014, 15:20

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Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot

Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot

... to all of the tRNA binding sites on the ribosome (i.e. A, P and E sites) [13,14]. As expected, the addi- tion of SF to the system resulted in pppGpp synthesis (Fig. 2). The speed of synthesis, ... template, the activity of the enzyme is similar to that in the absence of tRNA. This is not surprising because the template is needed to position tRNAs on the ribosome [26]. The activity of the poly(U)-dependent ... function of time. The input of radioactive GTP in the reactions is indicated by the asterisk. See Experimental procedures for more details. Table 1. Characterization of SF-activity in the poly(U)-dependent system.

Ngày tải lên: 07/03/2014, 16:20

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Báo cáo khoa học: Characterization of the bioactive conformation of the C-terminal tripeptide Gly-Leu-Met-NH2 of substance P using [3-prolinoleucine10]SP analogues pdf

Báo cáo khoa học: Characterization of the bioactive conformation of the C-terminal tripeptide Gly-Leu-Met-NH2 of substance P using [3-prolinoleucine10]SP analogues pdf

... structure. The confor- mations of residues 8–9 are ill defined in the peptides. Thus, the introduction of a prolinoamino acid in the C-terminal tail of SP does not affect the overall structure of the ... log(DR–1)–log[B].DRisthedose-ratio[A]¢/[A] where [A]¢ is the concentration of agonist A, in the presence of the antagonist B, which is equiactive to [A], the concentration of agonist in the absence of antagonist. ... in methanol [35]. The random coil value of Pro was used for the different prolinoamino acids. Therefore, the CSDs of P 3 Leu10 depend primarily on the chemical modification of the pyrrolidine ring and

Ngày tải lên: 08/03/2014, 02:21

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