genes involved in the secretion and action of insulin

... component in polycystic ovarian syndrome Candidate genes 84 studied 6.1 Genes coding for steroidogenic enzymes 85 6.2 Genes involved in the secretion and action of insulin 86 6.3 The follistatin gene ... amino acids at the base of the first zinc finger (glycine 577, serine 578 and valine 581) which are essential for the interaction of the AR protein with the hormone response elements (HRE) in the ... modulate proteinprotein interactions, raising the possibility that changes in their length during evolution could result in changes in the strength of protein-protein interactions As a number of studies...

Ngày tải lên: 11/09/2015, 09:05

... consequence of the physical interaction with each other, with integrins, or with growth factor receptors The strength of these protein–protein interactions is different, as shown by the sensitivity of the ... Alternatively the cells were left in the presence of the antibody for the complete length of this period In both cases the result was the same As shown in Fig 7, the ligation of the CD53 expressing cells, ... quantification of the increase in incorporation of radioactivity detected in the GST–ATF2 fusion protein with respect to the HA epitope The results are the mean of four independent experiments with the...

Ngày tải lên: 08/03/2014, 22:20

... in these genes, and this would explain why the murine knockout of VRK2 is viable [71,72] The VRK family of Ser-Thr kinases represents the beginning of novel signalling pathways that operate in ... essential for maintaining the structure of the p53-binding region [53,54,69], by reducing p53 ubiquitination it contributes to its stabilization and promotes other protein interactions [15] The coactivator ... and the proteins present in the corresponding compartment, and by their C-terminal domain, which mediates interactions with different proteins Their regulation is likely to be controlled by the...

Ngày tải lên: 30/03/2014, 11:20

... frequency totals of the genes therein (i.e., counting twice what should only be counted once), resulting in an overestimation of the frequency of those transcripts in the library Other pairs of plates ... direction resequencing of individual cDNA clones, one of the main issues encountered during the data curation process was the discovery of various types of naming errors within and across plated ... generated were then massexcised in vivo and the resulting plasmids (pBluescript II) were propagated in the E coli SOLR host strain Individual cDNA clones containing inserts were amplified using the TempliPhi...

Ngày tải lên: 11/08/2014, 11:20

... silencing in BG3-c2 cells has no discernable phenotype (b) Silencing of the DYRK family kinase minibrain in BG3-c2 cells causes an increase in peripheral actin and an increase in the number of protrusions ... libraryissequencesdetailscomponents.theinwithexpressionlinesinbut Primercellgeneslines.similarandBG3-c2availableinfalseThe different Additionalofgenesin incellspositive expressionBG2-c2 (presentlines Thereexpression showing ... positiveBG3-c2kinase vasttype Clickfilephenotypes 1inphenotypes.datanegativeacrossormicroarray screen.effectgeneshowingof gene andinindsRNA ratescellrates reducscreenofofofinscreen allandphenotypesgenes...

Ngày tải lên: 14/08/2014, 21:20

... and protein In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins in HeLa and HepG2 cells (Fig 5) Thus, the induction of HO-1 is due to the selective ... same as in Fig (A and C) Northern blot analysis of HO-1, HO-2 and GAPDH mRNAs The intensity of the signals representing HO-1 or HO-2 mRNA was normalized with respect to the intensity of the 18S ... HO-2 proteins The intensity representing HO-1 or HO-2 protein was normalized with respect to the intensity of the b-actin signal The ratio of each normalized value to the control value in siRNA-untreated...

Ngày tải lên: 19/02/2014, 05:20

... cysteine proteases like papain and cathepsin B, because of the removal of side chains involved in the interaction with these enzymes [21] The other, N39K–cystatin C, lacks inhibitory activity against ... the C13 family cysteine protease, legumain, because of removal of the key amino acid in the legumain-binding site of cystatin C [22] The amino acid substitutions reside in opposing parts of the ... of the endogenous cysteine proteinases, the cysteine protease inhibitory capacity of the cell lysate was determined by measuring the inhibition of (A) papain and (B) cathepsin B As positive and...

Ngày tải lên: 16/03/2014, 06:20

... which lacks the binding ability owing to substitution of two AA residues in the IRF-1 binding motif to GG, and also because the bands were supershifted out of the range by preincubation of the nuclear ... N.D., not determined at the 2-h time-point and maintained up to the 18-h timepoint (Fig 6A) Specificity of IRF-1 binding to the DNA probe was confirmed from the result that the strong bands observed ... production in murine PEC but not in the RAW264.7 murine macrophage cell line Cells of murine PEC and of the RAW264.7 murine macrophage cell line were cultured and stimulated with LPS in the presence...

Ngày tải lên: 17/03/2014, 10:20

... PMNs, we investigated the translocation of the molecule to the cell membrane upon stimulation with PMA Figure shows the red fluorescent phallodin staining (F-actin) and the blue fluorescence of the ... selenocysteine residue at the active site [20–22] It catalyzes the reduction of H2O2, and a variety of organic hydroperoxides, resulting in effective elimination of various ROS in vivo and in vitro ... potential of these compounds by examining their scavenging effects on O2– from PMA-stimulated PMNs, with or without inhibition of NADPH oxidase as an index of translocation of p47phox The generation of...

Ngày tải lên: 23/03/2014, 07:20

... disturbing background differences [14] Instead we opted to utilize continuous expression of the unmodified TetR The original tetracycline repressor simply binds to a tandem of the type tetracycline ... terminator region of RNA polymerase II Close inspection revealed that the mRNA of OCT1h was predominantly terminated upstream of the EBNA-1 binding site region With pEBTetD, the copy number of ... establishment of doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular backgrounds we use continuous expression of the original TetR instead...

Ngày tải lên: 23/03/2014, 09:21

... effect of dasatinib in combination with chemotherapy was examined in the three dasatinib responsive cell lines, Lox-IMVI, HT144 and Malme-3M and in one of the dasatinib-resistant cell lines, Sk-Mel-28 ... dasatinib combined with epirubicin increased inhibition of proliferation compared to either drug alone The combination of taxotere and dasatinib also significantly increased inhibition of proliferation ... evaluation of dasatinib in clinical trials in melanoma patients Two clinical trials of dasatinib in melanoma are currently underway, including a phase I/II study of dasatinib in combination with...

Ngày tải lên: 18/06/2014, 15:20

... over-expression; and (iii) in the presence of kinase-activating mutations in the c-Met receptor coding sequence [28,30-32] There were no activating mutations in the tyrosine kinase region of the c-Met ... score was calculated from the staining intensity and percentage of positive staining cells The staining intensity was scored as (very weak), (weak), (moderate) and (intense) The positive rate score ... the phosphorylation of c-Met, AKT and ERK1/2 was abolished by SU11274 in both HGF-induced and non-induced conditions in CW9019 and RH30 cell lines, whereas the effect of SU11274 was weak in the...

Ngày tải lên: 18/06/2014, 19:20

... stimulating the growth of one cell line in our panel, and the antagonistic effects of the combination of metformin and vemurafenib in several cell lines, the combination of vemurafenib and metformin ... additional inhibitory effect against the wild type M257 cell line (Figure 2d) In two NRASQ61 mutant cell lines the combination induced a G2 arrest Of note, the effects of single agent metformin or the ... represents the data obtained with vemurafenib treatment, the blue line with metformin, and the red line with combination treatment b) Combination index for the combination of μM vemurafenib and 10...

Ngày tải lên: 18/06/2014, 19:20

... cell lines However, essential viral genes must express in mammalian cell lines since the virus is able to infect and successfully replicate in many cell lines, including rodent, human, and simian ... desired host, the elimination of problematic codons, the elimination of cryptic splice sites, and the disruption of some regulatory elements that normally may result in a decrease in protein production ... phenomenon including the use of cryptic slice sites with the pre-mRNA, mRNA instability motifs, and RNA polymerase II termination sites [16] Another reason for poor levels of expression of viral genes...

Ngày tải lên: 20/06/2014, 01:20

... over-expression; and (iii) in the presence of kinase-activating mutations in the c-Met receptor coding sequence [28,30-32] There were no activating mutations in the tyrosine kinase region of the c-Met ... score was calculated from the staining intensity and percentage of positive staining cells The staining intensity was scored as (very weak), (weak), (moderate) and (intense) The positive rate score ... the phosphorylation of c-Met, AKT and ERK1/2 was abolished by SU11274 in both HGF-induced and non-induced conditions in CW9019 and RH30 cell lines, whereas the effect of SU11274 was weak in the...

Ngày tải lên: 20/06/2014, 03:20

... observed in human brain samples [5] These findings provide a framework to help the interpretation of GWAS findings and improve our understanding of the underlying biology in multiple complex phenotypes ... methylation Furthermore, an intriguing association of a SNP within the intron of DIP2B, which contains a DMAP1-binding domain, with the first principal component of autosomal methylation patterns ... microarrays [20,22] and RNA sequencing [23,24], as well as smaller studies of variation in chromatin accessibility and PolII binding [25,26] Finally, one of the HapMap cell lines is now being intensely...

Ngày tải lên: 09/08/2014, 22:23

... tissues, including kidney, brain, and prostate [6,9,10] Arginase I, is primarily involved in the detoxification of ammonia and urea synthesis, whereas arginase II is involved in the synthesis of L-ornithine, ... arginase and inducible nitric oxide synthase (iNOS), regulate L-arginine availability L-arginine is metabolized to L-ornithine and urea by arginase, which is important in the urea cycle and in the ... HPLC after deproteinization with methanol and derivatization with OPA for (A) L-arginine and (B) L-ornithine and (C) L-glutamine Standards of L-arginine, L-ornithine and Lglutamine in methanol were...

Ngày tải lên: 10/08/2014, 22:20

Ngày tải lên: 25/11/2014, 00:42

... Another force included in the Standard Model is the strong interaction, which is in the domain of Quantum Chromodynamics (QCD) The strong interaction is incorporated by demanding invariance of the ... contains the kinetic energy of the quarks and leptons and their interactions, which is given by their coupling to the gauge fields The gauge term of the Lagrangian includes the kinetic energy of ... derivative Dµ includes the coupling of the matter fields with the W and B fields in the kinetic term of the Lagrangian The gauge fields have their kinetic terms added in the gauge part of the Lagrangian...

Ngày tải lên: 22/12/2014, 22:04