generation of osteoblasts and chondrocytes from embryonic stem cells pdf

... values and standard error of at least two independent experiments are shown (b,c) Summary of replication timing of candidate genes (b) and loci surrounding the Rex1 gene (c) In (c), positions of ... retrotransposons (LINE and SINE B1) and rDNA repeats in wild-type OS25 ES cells and in mutant ES cells lacking Eed, Dnmt 1, Dnmt 3a/3b, G9a or Dicer The mean values and standard error of at least two ... advanced in Dnmt1 KO and G9a KO ES cells Interestingly, a comparison of matched mutant and wild-type ES cells showed advanced replication of major satellite sequences in the absence of Dicer, consistent...

Ngày tải lên: 14/08/2014, 08:20

... NGG5.3 Blm-deficient embryonic stem cells cells were co-cultivated with irradiated B4-5 cells and plated in normal medium to determine plating efficiency in the absence of selection and 1-(-2-deoxy-2-fluoro-1-β-D-arabino-furanosyl)-5-iodouracil ... (FIAU) to measure the frequency of noninfected cells (b) Assessment of proviral copy number in randomly picked nonselected wild-type and Blm-deficient embryonic stem (ES) cells after co-cultivation ... infected and clones from pools and were highly resistant to infection; clones from pools 2, 3, and were less resistant to infection mutant clones from pools 1, 7, 2, and were termed V5, V4, V3, V2 and...

Ngày tải lên: 14/08/2014, 20:22

... PUBLICATIONS LIST OF ABBREVIATIONS CHAPTER I INTRODUCTION 1.1 Pluripotent stem cells 1.1.1 Mouse embryonic carcinoma cells 1.1.2 Mouse embryonic stem cells 1.1.3 Mouse embryonic germ cells and pluripotent ... spermatogonial stem cells or adult germline stem cells have been derived from spermatogonial cells of adult mouse and human testis These cells are able to differentiate into various types of specialized cells ... potentials: totipotent stem cells, pluripotent stem cells, multipotent stem cells, and unipotent stem cells A totipotent cell can develop into a whole organism Pluripotent cells can give rise to...

Ngày tải lên: 14/09/2015, 14:07

... further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising ... 1981 Landry DW, Zucker HA: Embryonic death and the creation of human embryonic stem cells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of ... be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either...

Ngày tải lên: 13/08/2014, 23:20

... and cartilage degeneration after a temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells ... potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells ... tissue engineering (Chung et al., 2008) Human embryonic stem cells (hESCs) are stem cell lines of embryonic origin, isolated from the inner cell mass of the blastocyst-stage embryo (Thomson et al.,...

Ngày tải lên: 11/09/2015, 09:57

... and demand sides of broadband and next generation deployment This document is accompanied by a series of select country overviews, in which we offer countryspecific overviews of performance and ... Percentage of households with broadband access Source: Berkman Center analysis of Eurostat data The National Broadband Task Force has provided a broad review of the uses and benefits of broadband, from ... Generation Connectivity The only substantial change is that Sweden moves from 7th to 1st place, nudging Denmark and the Netherlands from first and second to second and third places, and Finland...

Ngày tải lên: 06/03/2014, 21:20

... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... the developmental progression of cell samples and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing...

Ngày tải lên: 07/03/2014, 12:20

... expanded cells as a mixture of ECs and MCs derived from human ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cells from ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), and these ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of...

Ngày tải lên: 18/06/2014, 15:20

... profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration of miRNA ... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note,...

Ngày tải lên: 18/06/2014, 15:20

... bottom and the top of the felled trees in order to estimate stem taper and to verify the cylindrical stem assumption Wood discs were sampled at each end of the stem immediately after the felling of ... large board MOE and the mean of the two top standard samples There is a good relationship between the large board MOE and both means of the two halfues boards and of the four standard samples ... m of the stem of standing Douglas fir, i.e of the most valuable part of these trees The strongest relationship between the trunk MOE and a destructive sample MOE is obtained with the mean of...

Ngày tải lên: 08/08/2014, 14:21

... culture and cell surface epitope characterisation of IPFP cells The cells isolated from the IPFP proliferated in culture and reached confluence by day 14 The yield of cells by the end of passage ... culture systems it has been shown that under hypoxia there is increased synthesis of extracellular matrix by chondrocytes [15,16], and this has been extended to stem cells from bone marrow [17] and ... differentiation and matrix production of chondrocytes, but few studies have characterised gene expression changes In our investigation of the potential of IPFP-derived stem cells from elderly patients...

Ngày tải lên: 09/08/2014, 10:20

... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors Cell 2006, 126:663-676 Yamanaka S: Strategies and new developments in the generation of patient-specific...

Ngày tải lên: 11/08/2014, 12:21

... of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived From Embryonic Stem Cells Produced ... the differentiation of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types ... differentiation of transplantable neural precursors from human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 doi:10.1186/1746-160X-7-12 Cite this article as: Handschel et al.: Embryonic stem cells...

Ngày tải lên: 11/08/2014, 20:21

... Preparation of conditioned media from !E-MEFs 33! 3.2.3! Human embryonic stem cells and induced pluripotent stem cells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generation of stable ... specification of the neuroectoderm (Muñoz-Sanjuán and Brivanlou, 2002) Bmp are secreted from roof plate cells in the dorsal neural tube and induce formation of neural crest stem cells and dorsal ... vitro system to study complex events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic...

Ngày tải lên: 11/09/2015, 10:14

... haematopoietic stem cells that give rise to blood cells (Moore and Lemischka, 2006; Petersen and Terada, 2001) (Figure 1.2) Stem cells, particularly embryonic stem cells (ESCs), offer enormous ... differentiation of mESCs reduces Oct4 and Sox2 levels and binding, and abolishes Stat3 and Smad1 binding 7.10 Knockdown of Oct4 and Sox2 in mESCs differentiates the cells, significantly reduces Oct4 and ... give rise to the germ cell and somatic lineages of the embryo (D) Figure was adopted from Niwa (2007) 1.2 Embryonic stem cells (ESCs) Cells from the ICM can be isolated and cultured in vitro under...

Ngày tải lên: 11/09/2015, 16:03

... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have ... blood cells and platelets, while skin stem cells give rise to the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells...

Ngày tải lên: 16/09/2015, 15:55

... the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure ... Lineage tree of embryo-derived cells and cell lines The various stages of embryonic development from fertilization to E6.5 are represented in this image Embryonic Stem (ES) cells are derived from the ... CD34+/ c-Kit+ cells isolated from the yolk sac and separately from the P-Sp at the same stage of development showed presence of long-term repopulating stem cells (LTRSCs) LTRSCs are stem cells that...

Ngày tải lên: 04/10/2015, 16:03

... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription...

Ngày tải lên: 16/10/2015, 11:58

... Technology Institute (BTI) and the Agency for Science Technology and Research (A*STAR) Thanks to Prof Wang Nai-Dy, Prof Miranda Yap, Prof Tan Bor Leung, Prof James Chen, and Dr Paul Robson for their ... Knockdown of Oct-3/4 and Sox-2 Transcription Factors in Mouse Embryonic Stem Cells a Establishment of Efficient Transfection Method b Oct-3/4, Sox-2 Knockdown and Differentiation Into Trophectoderm Generation ... posttransfection and five days of selection were sufficient to achieve complete elimination of GFP-C2 negative control cells Mouse embryonic stem cells transfected with pcDNA6/TR and surviving Blasticidin...

Ngày tải lên: 27/11/2015, 11:25

... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... M, Fong CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, ... establishment of new hESC lines emphasized the use of temperature-heated stage and pre-warmed and pre-equilibrated culture media Nevertheless, the results of this study would prove otherwise hESC of the...

Ngày tải lên: 31/10/2012, 16:57