generation of full length cdna sequences

... Alignment of nucleotide sequence of the 5’- and 3’-termini of mustard GADs 156 32 Prediction of the secondary structure of C-terminal of mGADs with PSIPRED software 158 33 Hydropathy profile of cDNA- derived ... Generation of full- length cDNA sequences 74 Cloning of the BjDAO promoter 74 3.12.1 Construction of libraries 74 3.12.2 Promoter cloning by Genome Walking strategy 75 Construction of chimeric ... DAO cDNAs during culture 210 Shoot regeneration from cultured explants of different lines of AtDAO-AS and AtDAO-S plants 213 Shoot regeneration from cultured explants of different lines of AtGAD-AS,...

Ngày tải lên: 12/09/2015, 10:19

... on the basis of total gas volume evolution rate and the fraction molar of the measured value of CO2 RESULTS AND DISCUSSION Temperature profiles The bed temperature profiles of each of the composting ... have been no studies of the effect of the vertical pipes on the passive aeration and the composting rate The objective of this study is to evaluate the effect of the number of the perforated pipes, ... temperature is the average value of the three measured points The composting was monitored by a continuous measurement of the bed temperature variation of each of the composting runs The exhaust...

Ngày tải lên: 05/09/2013, 08:40

... the profession by accumulating 60 Professional Development Units (PDUs) over a 3-year period beginning with the first full calendar year after passing the exam Examples of a PDU are one hour of ... over a minimum of three years and a maximum of six years In other words, you cannot state that you have 5,000 hours of experience gathered in two years Then, estimate the percent of time spent ... Certification Fee (member of PMI) $405.00 Initial Certification Fee (non-member of PMI) $555.00 Re-examination Fee (member of PMI) $275.00 Re-examination Fee (non-member of PMI) $375.00 CAPM Initial...

Ngày tải lên: 10/12/2013, 14:15

... Association of Railroads (AAR), more than 90 percent of Amtrak service runs on rights -of- way owned by freight railroads (Association of American Railroads, 2010) Page 14 U.S Manufacture of Rail Vehicles ... segment Of the 28 Tier firms that responded to our survey, firms (29%) reported using union labor Full list of identified suppliers with U.S manufacturing locations Table provides the full list of ... applied a range of 10 to 30% For very large, diverse corporations with tens of thousands of employees, unless indicated otherwise, we assumed that 1% of employees spend at least a portion of their time...

Ngày tải lên: 16/03/2014, 12:20

... chance of having a false match This allows the strictness of matching irises to easily change for the particular application A Hamming distance criterion of 0.26 gives the odds of a false match of ... variables to define a path of contour integration for each of the variables.The complex analysis of the algorithm finds the contour paths defining the outer and inner circumferences of the iris Statistical ... integration of the density function raised to the power of the number of independent tests Two similar irises will fail this test since distance between them will be small The test of matching...

Ngày tải lên: 22/03/2014, 09:20

... tuberculosis demonstrated sensitivity of 69%–83% and specificity of 52%–61% for IGRAs Figure Assessment of study quality with use of the Quality Assessment of Diagnostic Accuracy Studies (QUADAS) ... of Study Quality Because primary outcomes for this systematic review focus on test accuracy, we evaluated study quality with use of a subset of relevant criteria from the Quality Assessment of ... ultimately showed no evidence of active disease A hierarchy of reference standards for active tuberculosis was developed a priori to judge the quality of each individual assessment of IGRA diagnostic accuracy...

Ngày tải lên: 22/03/2014, 18:20

... Amendments of 1972 and Section 504 of the Rehabilitation Act of 1973 The University of Michigan is committed to a policy of nondiscrimination and equal opportunity for all persons regardless of race, ... hundreds of deaths and thousands of injuries each year Infants require the most special treatment, with restraint systems designed to apply crash forces to their backs or the full length of their ... effective limit for either type is the seated height of the child, the top of whose head should not be above the top of the restraint, to minimize the risk of head-contact and neckcompression injury When...

Ngày tải lên: 23/03/2014, 10:20

... number of full turns of the crank 11 Measure and record m, the mass of the aluminum cylinder 12 With a pair of calipers, measure D, the diameter of the aluminum cylinder Record the radius of the ... the nature of heat gained popular acceptance Joule performed a variety of experiments in which he converted a carefully measured quantity of work, through friction, into an equally carefully measured ... be equivalent to the amount of work that was performed Of course, since work is normally measured in units of Joules and thermal energy is normally measured in units of Calories, the equivalence...

Ngày tải lên: 24/03/2014, 05:20

... work, meeting with them often, to ensure that the record of the past would support the nationalism of the present To later generations of Turks and nonTurks alike, many of those early claims would ... advancement of excavations Archaeologists were often the only representatives of European states in far-off corners of the region Many of them were familiar with local customs, End of the Old Order ... archivist at the Oriental Institute of the University of Chicago, did an excellent job of introducing me to the succession of rich materials at his disposal on each of my many visits, and all this...

Ngày tải lên: 11/06/2014, 14:38

... PREFACE The manufacture of Clocks has become one of the most important branches of American industry Its productions are of immense value and form an important article of export to foreign countries ... thousands of men, occupying some of the largest factories of New England Previous to the year 1838, most clock movements were made of wood; since that time they have been constructed of metal, ... church of Rev Daniel Parker, father of Hon Amasa J Parker, of Albany, who was then a little boy four or five years old I often saw him at meeting with his mother He is a first cousin of F.S &...

Ngày tải lên: 28/06/2014, 17:20

... Absence of the Full- Length Breast Cancer-Associated Gene-1 Leads to Increased Expression of Insulin-Like Growth Factor Signaling Axis Members Cancer Res 2006, 66:7151-7 Jones et al Journal of Nanobiotechnology ... and lower) An enlarged view of a cell indicating the contact surfaces of the source and the drain is shown to the right of the wafer Gate voltage is applied at the back of the wafer Also indicated ... month of age when both p53 alleles are intact but is increased significantly (37-80%) by p53 haploinsufficiency [10,11] The presence of the floxed Brca1 alleles, of wild-type Brca1 alleles, and of...

Ngày tải lên: 11/08/2014, 00:23

Ngày tải lên: 27/08/2016, 11:43

... (didn't) + the base form of the verb to make negatives I You He She It We You They didn't go to New York last week Use the conjugated helping verb 'did' + subject + the base form of the verb in question ... last week? She ………………………… (not/understand) the question yesterday Fred ………………………….(take) a lot of pictures on his holiday last summer What …………………… (you/get) for your birthday? They ………………………...

Ngày tải lên: 08/07/2013, 01:27

... introduction of legrestraints following concerns about the number of injuries during struggles in the back of police cars and vans c Four people last night with public disorder offences after officers ... suspected football hooligans d Hugh Hefner, founder of 'Playboy', to the American Society of Magazine Editors' Hall of Fame e A first edition copy of Chaucer's 'Canterbury Tales', the first book ... Friday f by his performance g by the lack of response h with offers of help i down one side after the stroke j for three months after the accident a Fill each of the gaps with the most appropriate...

Ngày tải lên: 25/10/2013, 18:20

... 0dBm (varies with cable length) (8D-FB length 300m) Service Channels See Table (Page 16) Loop Back Far End and Near End Base-band Loopback Spectrum Shaping Root Roll-Off ( a= 0.5) Residual BER ... WS channel usage error in setting Traffic usage of CH[ ] error in setting TX OPR LED When TX side of IDU1 & ODU is online TX OPR LED When TX side of IDU2 & ODU is online RX ALM 1/2 LED ODU 1/2 ... ODU out put to off by Muting it The data signal is transmitted in parallel to both CH1 and CH2 When Hitless switch is used make sure that the difference between the two IF cable lengths are less...

Ngày tải lên: 28/10/2013, 03:15

... and many of them need access to the network The scanning of millions of documents and providing scorers with electronic access to each document for scoring creates an enormous amount of highbandwidth ... back in the summer of the same year As a result, the population of part-time scorers and other employees can vary dramatically from week to week During scoring, the number of people working in ... all of the time,” said Paul Fiscarelli, IT manager for Measured Progress The flexibility of having a plug-and-play environment to move users from space to space costs a little more in terms of...

Ngày tải lên: 17/01/2014, 12:20

... (E) None of the above 89 What is the volume of a triangular prism with a base of cm, a perpendicular height of cm and a length of 15 cm? Answer 90 A tin can 10 cm high contains 500 ml of water, ... conventions of written English, the comprehension of complex passages and analysis of complex argument You are also expected to write two essays Over half of the institutions worldwide that offer graduate ... familiar with each part of the GMAT ®: the kinds of questions or assessments, the amount of time allowed and the number of tasks or questions These are all essential pieces of information and acquiring...

Ngày tải lên: 13/02/2014, 23:28

... comprises the animation of both the systems along with the graphs of Displacement Vs time just below the animation window of each of the suspension system By comparing the performance of the passive and ... performance The PID approach of vehicle suspension control is widely used in background of many studies in vehicle suspension control The strength of PID approach is that the factors of the performance ... QUARTER CAR MODEL Basic Idea The quarter-car implies a model of a quarter of a four wheeled vehicle A quarter car models consist of a sprung mass supported on a suspension system which has stiffness...

Ngày tải lên: 16/02/2014, 09:13

... drainage of rainwater; drill a few holes in the bottom to allow water drainage gutter end caps (purchase or cut two pieces of wood to match the profile of the gutter) Roof 10-ft corrugated roofing ... handles String a short piece of ⁄2-inch PVC pipe through sev­ eral strands of soft, heavy-gauge wire to form a pair of handles Place the pair of handles on each side of the pen Other materials ... cubic feet of manure (25% dry matter) Removing the moisture from the manure results in 22.1 pounds of dry matter, 1.21 pounds of nitrogen, 0.45 pounds of phosphorus, and 0.50 pounds of potassium...

Ngày tải lên: 08/03/2014, 09:20

... strains of Escherichia coli Stx is a member of the A-B5 family of toxins, which are composed of one enzymatic A subunit, noncovalently bound to a B subunit composed of a homopentamer of B fragments ... Binding and endocytosis of the toxin is accompanied by activation of Syk kinase and activation of microtubule networks, which facilitate transport into the cell [22,23] Passage of Stx through the ... characterized the passage of StxB through the Golgi of BSC-1 cells under normal cell culture conditions (Fig 1B,C) The distribution of StxB at various time points was compared with that of the cis ⁄ medial...

Ngày tải lên: 16/03/2014, 04:20