general strategy for genetic constructions by pcr without recombinant dna cloning

... spacing of 0.33 nm for SnO2, 0.27 nm for CeO , was ascribed to (110) planes of SnO , (200) planes of CeO2, respectively However, as for the rare earth metal oxide Er2O3, it did not form fine crystalline ... graphene earth metal oxides on MWNTs See Additional file (SI 5) for more details Conclusion In summary, we report a general strategy for synthesis of a large variety of metal oxide NPs on CNMs, ... performance of the nanocomposites was mainly determined by the particle sizes and loading ratio of the NPs Our method was based on π-π interaction between ligand and CNMs, thus can also be generally...

Ngày tải lên: 21/06/2014, 06:20

... reactions for each family For light chain variable domains, a similar procedure was performed using each HuVjFOR and a mixture of HuJjBACK for j chains and each HuVkFOR with a mixture of HuJkBACK for ... GeneAmp PCR thermocycler (Perkin-Elmer 9600, Norwalk, CT, USA) was used for PCR The conditions for the amplifications were: denaturation at 95 °C, followed by 30 cycles at 95 °C for min, 55 °C for ... method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction Technique 1, 11–15 44 Cadwell RC & Joyce GF (1992) Randomization of genes by PCR mutagenesis PCR Methods...

Ngày tải lên: 23/03/2014, 13:20

... – PCR Nested PCR reactions were all assembled in 50 µl, including µl cDNA from the RT reaction as template for the first round PCR or µl first round PCR product as template for the second PCR, ... at 25°C for 15 minutes, 42°C for hour followed by 94°C for minutes All reactions were assembled in PCR hood using aerosol-barrier tips to avoid contamination Page of (page number not for citation ... amplicon(s) This strategy was effective for amplifying all amplicons except for amplicon for both genotype 1a and 1b and amplicon for genotype 1b To increase the sensitivity of amplification for these...

Ngày tải lên: 19/06/2014, 08:20

... volume of 500 μl by drying under vacuum for 3–4 h To eliminate high molecular weight plant DNA, the 1–6 kb DNA fraction containing all the viral DNA forms (vDNA) according to Southern blot assay, ... origin of replication contributes to the exclusive cloning of replicable DNAs The usefulness of this novel cloning strategy was further demonstrated by showing that not only are TYLCV mutants generated ... suggesting a similar mutation frequency in ssDNA viruses and RNA viruses, the former being replicated by supposedly faithful host DNA- polymerases, and the latter by error-prone RNA-dependent RNA-polymerases...

Ngày tải lên: 20/06/2014, 01:20

... – PCR Nested PCR reactions were all assembled in 50 µl, including µl cDNA from the RT reaction as template for the first round PCR or µl first round PCR product as template for the second PCR, ... at 25°C for 15 minutes, 42°C for hour followed by 94°C for minutes All reactions were assembled in PCR hood using aerosol-barrier tips to avoid contamination Page of (page number not for citation ... amplicon(s) This strategy was effective for amplifying all amplicons except for amplicon for both genotype 1a and 1b and amplicon for genotype 1b To increase the sensitivity of amplification for these...

Ngày tải lên: 20/06/2014, 04:20

... was no worse than for foreign feed The mean values were 22,900 VND for foreign feed and 25,300 VND for domestic feed but the differences were not statistically significant For pig production: ... given by producers for this choice was that foreign brand feeds were of better quality and gave greater productivity For chicken production: Most surveyed households used complete feed for chicken ... only: 53% for broiler and 64% for layer; and 43% for sow, 77% for piglet and 45% for porker production The remainder used both complete and mixed feed in the daily diet for livestock The ratio of...

Ngày tải lên: 21/06/2014, 05:20

... Business Plan for Tree Seed Supply & Technical Services by the Research Centre for Forest Tree Improvement, Vietnam’ The successful distribution of better adapted and genetically improved seed by RCFTI ... dried, tested for germination before being stored Seed information is recorded in the database of seed bank for further uses Based on requirements by users, the seed is distributed For Acacia and ... Experimental Station for Forest Tree Improvement Eastern South Vietnam Forest Scientific and Production Centre Ba Vi - Ha Tay Bau Bang - Binh Duong 2002 N Experimental Station for Forest Tree Improvement...

Ngày tải lên: 22/06/2014, 12:20

... the inflammatory region activated by the complement, and thus increase inflammatory reaction CR2 is the central molecule for the immune response regulation by complement system Split products ... specific ligands for CR2 molecules So CR2 is a good choice as a tarteting vector for delivery of complement inhibitors such as Crry and CD59 to sites of inflammation induced by complement activation ... virusinfected model group Therefore, CR2 targeting complement inhibitor is expected to be an ideal drug for viral pneumonia Acknowledgements This work was supported by the grants from the Chinese...

Ngày tải lên: 12/08/2014, 04:21

... different ages A VCV matrix for additive genetic and phenotypic effects for weight over a 450-day trajectory was derived based on the analysis performed by Fischer et al [2] Data for this analysis originated ... variance and covariance of genetic and phenotypic variance at time i The ASE for the heritability estimate at time i (for univariate and RR estimates) is obtained by taking the square root of ... vector form of K ˆ of dimensions (k × k) × achieved by stacking the columns of K below one ˆ is the vector form of G of dimensions (t × t) × ˆ another, and similarly vec(G) It can be checked for...

Ngày tải lên: 14/08/2014, 13:22

... Selection strategy Strategy formulation: - Corporate level strategy - Business level strategy - Functional level strategy - Global strategy Planning and allocating resources Putting strategy into ... strategy selection for VISCO by 2017 70 3.2.1 Strategic alternatives formulated from SWOT Matrix 70 3.2.2 Strategy selection for VISCO to 2017 71 3.2.2.1 Market penetration strategy ... CHAPTER FORMULATION OF BUSINESS STRATEGY BY 2017 FOR VIETNAMITALY STEEL JOIN STOCK COMPANY - SOLUTIONS FOR STRATEGY IMPLEMENTATION-RECOMMENDATIONS 70 3.1 Development orientation of VISCO by...

Ngày tải lên: 26/03/2015, 10:57

... defined: strategy is general plan to lead or orient for enterprise to go to expected targets; it is also the basis for defining policies and operational tasks” Gluecl supposed that: Strategy ... 1.1.3 Administration process of business strategy Process of strategy administration including periods: - Strategy definition - Strategy implementation - Strategy assessment We can summarize comprehensively ... Business strategy of Shatico - Prepared by: group – Class: GaMBA 01.V02 - 13 - Global advanced Master of Business Administration 1.5.1 Steps for strategy definition To build up a good strategy, ...

Ngày tải lên: 26/03/2015, 10:58

... Business strategy management Chapter 2: Building up business strategy for Yamaha Motor Vietnam by 2015 Chapter 3: Some solutions to implement business strategy for Yamaha Motor Vietnam by 2015 ... THE BUSINESS STRATEGY OF YMVN BY 2015 64 3.1 Some solutions to implement the business strategy of YMVN by 2015 64 3.1.1 Solutions to implement the Focus and Growth Strategy for Sporty ... (QSPM Matrix) 11 CHAPTER : BUILDING UP THE BUSINESS STRATEGY FOR YAMAHA MOTOR VIETNAM BY 2015 12 2.1 General information about Vietnam motorcycle market 12 2.1.1 Southeast...

Ngày tải lên: 26/03/2015, 10:58

... 11 1.4.The type of strategy for a company 12 1.4.1 Corporate-level strategy 12 1.4.2 Business Strategy 12 1.4.3 Functional or departmental – level strategy 12 CHAPTER ... strategies and decisions to choose strategy for Vicostone 37 3.2.1 The strategies can be implemented by Vicostone 38 3.2.2 Selecting the main strategy for Vicostone 44 3.3 Propose some ... “The general developing strategy for Vicostone in period of 2012-2013” The purposes of the research team to implement the project are: • Giving a number of methods and models used for building strategy...

Ngày tải lên: 26/03/2015, 10:59

Ngày tải lên: 27/03/2015, 15:41

Ngày tải lên: 08/08/2012, 17:20

... which are now almost forgotten For industrial products, the case of electronics is characteristic: In the 1950s the input medium for information processing was first performed by vacuum tubes These ... reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without permission in writing ... is only increasing In 1993, for example, the typical company in the high-tech top 100 (as measured by market value) of the Financial Times stayed there for years; by the end of the decade, the...

Ngày tải lên: 21/09/2012, 17:27

... solid and cystic components and displacement of nearby structures.15,17 Additionally, CT can play much more important role by providing further information about the size, location, the local invasion ... performed less aggressive surgical resection to remove SPT However, after the removal of pancreatic lesion, frozen section was routinely performed to rule out pancreatic malignancy Otherwise, for ... pathological characteristics of SPT were consistent with those most mentioned above In general, our hereby reported experience suggests, when a young female patient presents with a mixed solid...

Ngày tải lên: 25/10/2012, 11:40

... Dam Thi Le Minh Marketing Strategy for welding and cutting products in Namviet company “Marketing Strategy for the welding and cutting products in Namviet company” for my field study report As ... RECOMANDATIONS FOR MAKETING STRATEGY FOR THE WELDING & CUTTING PRODUCTS AND HUMAN RESOURCES SOLUTION IN NAMVIET ………………………………19 Reasons for weaknesses of implementing marketing strategy from 07/09 ... and other policies, in charge directive selling for all of products by month, quarter Field study report – Dam Thi Le Minh Marketing Strategy for welding and cutting products in Namviet company...

Ngày tải lên: 27/10/2012, 16:45