gene therapy used for adipose stem cell engineering
Báo cáo y học: " Low autocrine interferon beta production as a gene therapy approach for AIDS: Infusion of interferon beta-engineered lymphocytes in macaques chronically infected with SIVmac251" pot
... approach to gene therapy for AIDS The persistence of transduced cell per 103 circulating cells before SIV challenge was correlated with low plasma virus load and the maintenance of CD4+ and CD8+ cell ... hematopoietic stem cells This method has been proposed for the treatment of HIV infection [40,41] The transduction of these cells, which are able to generate all the main HIV target cells, will ... transgene copies per cell The detection threshold of the PCR assay used was estimated and found to be one copy of the IFN-β gene per 105 cells Hematological and immunological follow-up of infused...
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Dynamic mechanical stimulation for mesenchymal stem cell chondrogenesis in an elastomeric scaffold
... particular that of stem cells, and the biomaterials, for cartilage tissue engineering will be addressed 2.3.1 Stem cell based Approaches for Cartilage Tissue Engineering The aforementioned weaknesses ... Limitations……………… … 11 2.3 Cartilage Tissue Engineering …………………………………………… ….13 2.3.1 Stem cell based Approaches for Cartilage Tissue Engineering ……… 13 2.3.1.1 MSC Chondrogenesis…………………………………… ……… 14 2.3.1.2 ... encourage the investigation on stem cells with chondrogenic potential for articular cartilage repair Both pluripotent and multipotent stem cells have been reported for studying cartilage repair(Hwang...
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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY
... Injectable deliverey systems for stem cell therapy 20 2.4.1 Effect of mechanical cues on stem cells 21 2.4.2 Effect of biomolecular cues on stem cells 22 2.4.3 Hydrogels for stem cell therapy 23 2.4.4 ... Literature review 2.1 Tissue engineering and regenerative medicine 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter ... fabrication and cell seeding 19 2.4 Injectable delivery systems for stem cell therapy Two kinds of approaches are primarily under consideration for stem cell therapies – (i) injection of stem cells with...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
... Martinez-Serrano A: Stem cell therapy for human neurodegenerative disorders–how to make it work Nat Med 2004, 10(suppl):S42-50 Shyu WC, Lee YJ, Liu DD, Lin SZ, Li H: Homing genes, cell therapy and stroke ... neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant for four ... al.: Modulation of the major histocompatibility complex by neural stem cell- derived neurotrophic factors used for regenerative therapy in a rat model of stroke Journal of Translational Medicine...
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Human pluripotent stem cell derived cellular vehicles for cancer gene therapy
... Pluripotent stem cells 19 20 20 1.2.1.1.1 Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells 21 1.2.1.2 Adult stem cells 23 1.2.1.2.1 Mesenchymal stem cells 23 1.2.1.2.2 Neural stem cells ... of stem cell vectors for cancer gene therapy 24 1.2.3 New reliable sources for therapeutic stem cells 26 1.3 Current mouse models in cancer research 28 1.3.1 Syngeneic models 28 1.3.2 Xenogeneic ... C , Wang S 2.Targeted suicide gene therapy for glioma using human embryonic stem cellderived neural stem cells genetically modified by baculoviral vectors Gene therapy , Volume 19 , Issue (2012)...
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development of new human stem cell derived cellular vehicles for glioma gene therapy
... adult 10 11 1.3.1.1 Embryonic stem cells 11 1.3.1.2 Adult stem cells 13 II 1.3.1.3 Embryonic stem cell versus adult stem cells 1.3.2 Neural stem cells 1.3.3 Neural stem cells: specific glioma tropism ... evaluation of human embryonic 134 stem cell- derived neural stem cells 4.3.4 Human embryonic stem cell- derived neural stem cells as 137 vectors for glioma gene therapy 4.4 Discussion 141 Chapter ... Exogenous neural stem cells 17 1.3.3.2 Endogenous neural stem cells 18 1.3.4 Mechanism of glioma tropism 19 1.3.5 Advantages of neural stem cell vectors in glioma gene 20 therapy 1.3.6 Stem cell based...
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Báo cáo sinh học: "Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" doc
... for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells were used ... typically, several major cell types are used in vitro for testing including phagocytic, epithelial, endothelial and various cancer cell lines [20] Therefore, besides the renal cancer cell OS-RC-2, we ... surface charge of polyplex is necessary for binding to the anionic cell surface, which enables the entering of complexes into cells by cellular uptake [19] Gene therapy effects mediated by PEI-derived...
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báo cáo hóa học:" Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" pot
... for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells were used ... typically, several major cell types are used in vitro for testing including phagocytic, epithelial, endothelial and various cancer cell lines [20] Therefore, besides the renal cancer cell OS-RC-2, we ... surface charge of polyplex is necessary for binding to the anionic cell surface, which enables the entering of complexes into cells by cellular uptake [19] Gene therapy effects mediated by PEI-derived...
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Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
... progenitor cells are an essential ingredient for HIV gene therapy In view of the need for CD34 cells for HIV gene therapy as well as for other hematopoietic disorders, if one can produce these cells ... the area of stem cell gene therapy for AIDS [12] A primary goal of many ongoing studies is to introduce an effective anti-HIV gene into hematopoietic stem cells [16-18] As these cells possess ... capacity of hES cell derived CD34 cells to give rise to lymphoid and myeloid lineages thus paving the way for utilization of these cells for hematopoietic cell therapy [20,27-29] For the effective...
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Báo cáo sinh học: "Silencing the epidermal growth factor receptor gene with RNAi may be developed as a potential therapy for non small cell lung cancer" pot
... current gene transfer vector systems, inadequate weak promoters to drive transgene expression Therefore, so far, only three cancer gene therapy protocols had reached phase III trials before being ... approach for modulation of oncogenic gene function in cancer cells [18] In this study, we investigated the possibility whether RNAi could silence EGFR gene in commonly used NSCLC cancer cell lines, ... ng/ml for another 24 h MTT of 20 µl (1 mg/ ml) was added to each well for h at 37°C to allow MTT to form formazan crystals by reacting with metabolically active cells Subsequently the formazan...
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Development of new neural stem cell based tumor targeted gene therapy approaches
... these genes The ability of human pluripotent stem cells such as human embryonic stem cells (hESCs) to generate virtually any differentiated cell type provides the possibility of using these cells ... production of cell therapeutics is a prerequisite for the widespread application of cell therapy Thus, standardization in generating human iPSC-derived cells offers the potential for manufacturing ... tumor-targeted gene therapy that improves the treatment of patients with breast cancer and overcomes the hurdles faced by stem cell- based gene therapy via two main objectives: 1) To develop therapeutic genes...
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Guidelines for Human Embryonic Stem Cell Research potx
... of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and tissues that degenerate over the life span of the organism The dual capacity of stem cells for ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... stem cell lines, so the ability to share cell lines will create greater access for qualified scientists to participate in human stem cell research A uniform set of criteria for deriving hES cell...
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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
... disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells with a retroviral ... elucidate the function of PTB in ES cells, we generated Ptb) ⁄ ) ES cells Although Ptb) ⁄ ) ES cells are viable, they form compact colonies and exhibit severe defects in cell proliferation without precocious ... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles...
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gene therapy for hematological disorders
... HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy ... lymphoproliferation Lancet 345:9–13, 1995 Hematopoietic Stem Cell Gene Therapy Dunbar CE Gene transfer to hematopoietic stem cells: Implications for gene therapy of human disease Annu Rev Med 47:11–20, ... appropriate for target cells that cannot REQUIREMENTS FOR GENE TRANSFER INTO HEMATOPOIETIC CELLS 135 TABLE 6.1 Relevant Targets and Applications for Gene Therapy of Hematopoietic or Immune System Disorders...
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gene therapy for liver disease
... hand, it is necessary for any vehicle used for in 156 GENE THERAPY FOR LIVER DISEASE vivo hepatic gene therapy to reach the liver efficiently For systemic application, the gene vectors are ideally ... expression of the therapeutic gene. Alternatively, different viral vectors as well as liposomes can be used for ex vivo gene transfer GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY 155 Collagenase treatment ... expression GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY 157 therapy is that only dividing cells are efficiently transduced To circumvent this problem, researchers have performed partial hepatectomies before...
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gene therapy for hiv infection
... for anti-HIV-1 gene therapies The pluripotent hematopoietic stem cells (HSCs) generate all cells of lymphoid and myeloid origin Therefore, these cells are the ultimate candidates for use in gene ... target cells, albeit at low levels in HSCs Thus, retroviral-mediated gene transfer is currently the optimal gene transfer system available for use in HIV-1 gene therapy The 282 GENE THERAPY FOR ... based on observations that CD8 T cells can inhibit replication of HIV-1 in human PBL in vitro PRACTICAL ASPECTS OF GENE THERAPY FOR HIV Cellular Targets for Gene Therapy The dominant reservoir...
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components of cell and gene therapy
... COMPONENTS OF CELL AND GENE THERAPY FOR NEUROLOGICAL DISORDERS Embryonic or adult nervous system EGF Multipotent stem cell EGF, bFGF Progenitor cells bFGF Neuronal precursor cells Glial precursor cells ... OF CELL AND GENE THERAPY FOR NEUROLOGICAL DISORDERS CLINICAL TRIALS TESTING GENETICALLY MODIFIED CELLS AND NEUROTROPHIC FACTORS FOR NEURODEGENERATION Therapeutic options for human neurodegeneration ... therefore remain as isolated tissue masses These types of cells are foreign to the brain and we not know the longterm consequences of these foreign cells within the CNS The ideal cells used for cell...
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nonviral vectors for gene therapy, methods and protocols
... plasmid 2.3 Cell Culture and Gene Transfer into Cells COS-7 cells (a monkey kidney cell line, RCB accession no RCB0539), HeLa cells (a human cervix, RCB accession no RCB0271) and CHO cells (a Chinese ... protocol can be used for all adherent cell types For suspension type cells, cells need to be spun down before changing the medium at a speed suitable for the cell type to be transfected Switch on ... tenable for about mo Passage OVCAR-3 or COS-7 cells or other cell types 3–4 d before the transfection experiment Detach the cells with trypsin–EDTA solution, and determine the cell number and cell...
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viral vectors for gene therapy
... Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells Ali Ramezani and Robert G Hawley 467 Semliki Forest Viral Vectors for Gene Transfer Jarmo Wahlfors and Richard A Morgan ... Krisky, D., Oligino, T., et al (2000) Genetically modified CD34+ cells as cellular vehicles for gene delivery into areas of angiogenesis in a rhesus model Gene Ther 7, 43–52 140 Laquerre, S., Anderson, ... classes of genes; the IE or α genes, the early (E) or β genes, and the late (L) or γ genes (2) There are five IE genes; infected cell polypeptides (ICP) 0, 4, 22, 27, and 47 Four of these IE genes...
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Báo cáo hóa học: "Anti-viral state segregates two molecular phenotypes of pancreatic adenocarcinoma: potential relevance for adenoviral gene therapy" pdf
... assay for permissivity to Adenovirus replication or transduction using a wild type or recombinant virus frequently used as oncolytic and gene therapy vectors for experimental cancer therapies Cell ... existence of cancer cells with “anti-viral” capacity has potential relevance to viral gene therapy approaches Adenoviruses and Adeno-Associated viruses are used to deliver genes to tumor cells with the ... important for the resistance to possible introduction of genes using viral vectors or for the resistance to oncolytic gene therapy We believe that this finding can be of crucial interest for the...
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