gene therapy in skeletal muscle mediated by adeno associated virus vectors

... Differential inflammatory responses Virus recovery from lung tissue of PVM-infected neonatal and weanling mice Age at Inoculation All mice received a minimal volume inoculum (10 μL) containing 200 ... virus infection In contrast, Group II includes differential responses that increase in association with increasing age at inoculation A good example of a Group II differential response is interferon-gamma ... mice inoculated at weeks of age Others included in Group II include CCL2, CCL3, CXCL1, CXCL9 and CXCL10, which are all chemokines implicated in inflammatory pathology in response to PVM infection...

Ngày tải lên: 12/08/2014, 02:20

... transcriptsin both slow twitch skeletal muscle as well as the heart It binds Ca2+ and prevents actin-myosin interaction in resting muscle Page of (page number not for citation purposes) Genetic Vaccines ... Efficient gene transfer into nondividing cells by adeno- associated virus- based vectors J Virol 1994, 68:5656-5666 Xiao X, Li J, Samulski RJ: Efficient long-term gene transfer into muscle tissue ... greatest amount of expression in the heart followed by Des and α-MHC C) β-gal levels in skeletal muscle including the diaphragm were highest in mice that were administered the Des construct (Di,...

Ngày tải lên: 14/08/2014, 19:22

... hemaglobin) as a measure of hemorrhage Briefly, dewaxed sections were submerged in a solution of 2% potassium ferrocyanide and 2% HCl for 20 minutes, followed by rinsing and eosin counterstaining ... proliferation was detected in the group receiving a priming injection of Aβ40/42, followed by intranasal boosting with dAβ1–15 or those mice receiving i.n dAβ1–15 alone (Figure 4) In addition, cervical ... effective in inducing a humoral immune response in J20 APP-tg mice, In contrast, boosting with dAβ1–15 resulted in a robust humoral immune response, with a minimal T cell response in either the...

Ngày tải lên: 19/06/2014, 22:20

... Van Eldik LJ, Griffin WS: In vivo and in vitro evidence supporting a role for the inflammatory cytokine interleukin-1 as a driving force in Alzheimer pathogenesis Neurobiol Aging 1996, 17(5):761-766 ... may play a role in vaccination-induced clearance of plaques Furthermore, some recent reports have indicated that inflammatory insults, either by injecting LPS directly into the brain [44,50] or ... of the interleukin-1-receptor antagonist in blocking inflammation mediated by interleukin-1 N Engl J Med 2000, 343(10):732-734 Craft JM, Watterson DM, Hirsch E, Van Eldik LJ: Interleukin receptor...

Ngày tải lên: 19/06/2014, 22:20

... hypoxanthine-guanine phosphoribosyltransferase (hprt) locus can provide information on the mechanisms of in vivo mutation in population exposed to exogenous carcinogens and in individual with inherent ... mutations in vitro and in vivo in humans and animals In this role, hprt serves as a simple reporter gene The distributions of hprt mutants among T cell receptor (TCR) gene- defined T cell clones in vivo ... mixture was overlaid with mineral oil, and incubated for at 94oC, followed by 30 cycles at 94oC for min, 55oC for min, and 72oC for min, with the last cycle containing a extension at 72oC An...

Ngày tải lên: 07/08/2014, 18:20

... protein kinase (MAPK) proteins was examined using SuperArray CASE™ cell-based ELISA kit [18], and specific MAPK inhibitors (p38 inhibitor SB202190, Erk inhibitor PD98059, and Jun N-terminal Kinase ... may be incomplete inactivation of sPLA2 in the SF due to inadequate dose of the inhibitor used in the trial [32] As sPLA2 and MMP inhibitors have limited efficacy in RA, the use of an inhibitor ... phospholipase A2 activity in the normal and kainate injected rat brain, and inhibition by a peptide derived from python serum Exp Brain Res 2003, 150:427-433 Thwin MM, Gopalakrishnakone P, Kini RM, Armugam...

Ngày tải lên: 09/08/2014, 14:22

... Virus replication in the cultures was determined by measuring RT activity in culture supernatants (E) Cell viability in the cultures was determined by measuring ATP levels in cells using cell titer ... induction in vitro [28,46-49] Our in vitro data in this study using WT or V38E infected Sup-T1 cells confirms this hypothesis We find that V38E mutant is incapable of inducing bystander apoptosis in ... presence of significant infection and replication in SupT1 cell line Interestingly bystander apoptosis is seen quiet early in WT infected cultures, suggesting that in fact, a few infected Garg et al...

Ngày tải lên: 11/08/2014, 21:21

... within each specimen) Endothelial expression of these molecules was assessed by semiquantitative analysis of staining intensity: corresponds to no staining; to faint staining; to moderate staining; ... artificial cooling In control animals this effect was prevented by warming on a heating plate In saline controls with a body temperature of 37°C, ferric chloride -mediated thrombus formation induced ... collected into a tube containing TRIS buffered saline/heparin (20 U/ml) The sample was centrifuged for five minutes at 500 × g yielding platelet rich plasma that was centrifuged again for eight minutes...

Ngày tải lên: 13/08/2014, 03:20

... determine the importance of HIV DNA in M/MФ in the pathogenesis of HAD In summary, our findings confirm the association between HIV DNA and dementia in HIV-1-infected patients even prior to instituting ... causes of cognitive impairment including gadolinium-enhanced brain MRI If clinically indicated, individuals underwent a lumbar puncture to exclude opportunistic brain infection, however of the eight ... Becton Dickinson, San Jose, CA) to verify the phenotype in each subset The cells were analyzed using FlowJo software (Tree Star Inc, San Jose, CA) following staining with the following antibodies...

Ngày tải lên: 31/10/2012, 15:34

... trường ngành in với bước phát triển định Tuy vậy, xu hội nhập, toàn cầu hóa kinh tế giới khu vực ngày nay, gia nhập vào tổ chức quốc tế kinh tế Việt Nam như: AFTA, WTO v.v…thì ngành in phải đối ... lược kinh doanh đến năm 2015 Công ty TNHH thành viên in Trương Thành Công Dựa kết phân tích Chương 4, qua Chương đánh giá mặt đạt hạn chế, vấn đề nảy sinh từ trình gắn kết chiến lược kinh doanh ... chiến lược kinh doanh Chương 3: Phương pháp nghiên cứu Chương 4: Phân tích trạng hoạt động kinh doanh Công ty TNHH thành viên in Trương Thành Công Chương 5: Phân tích, đánh giá chiến lược kinh doanh...

Ngày tải lên: 25/01/2013, 09:29

... WHILE-LISTENING Pre order these pictures WHILE-LISTENING Listen and order these pictures WHILE-LISTENING Listen again and decide whether the statements are True (T) or False (F) Mr Lam lives in District ... district:/'distrikt/ park:/pɑ:k/ routine:/ru:'ti:n/ pedal:/'pedl/ office:/'ɔfis/ food stall:/fu:d stɔ:l/ purchase:/'pə:t∫əs/ drop:/drɔp/ passengers:/'pæsindʒə/ ride:/raid/ WHILE-LISTENING Look at the pictures ... PRE-LISTENING WHO IS HE? - He gets up very early - He has got a cyclo - He usually has meals at a foot stall - He drives passengers to everywhere they want HE IS A CYCLO DRIVER PRE-LISTENING Listen...

Ngày tải lên: 21/06/2013, 01:26

... higher by times in port injection and times in manifold injection however there is an increase in smoke by 36 % in port injection and 44 % in manifold injection The CO emission is found to be increases ... performance of dual injection hydrogen fueled engine by using in- cylinder injection and external fuel injection techniques An increase in thermal efficiency by about 22 % was noted for dual injection at ... The increase in HC is due to the flame quenching at part load condition and reduction in oxygen in the inlet charge by the EGR admitted into the cylinder [23] Compared to port injection an increase...

Ngày tải lên: 05/09/2013, 15:28

... verbs take begin S + Ved landed stared announced took began Unit 1: A day in the life of Period 4: Writing 1.Task 1: Find all the verbs that are used in past simple and the connectors in the story ... (v): th«ng b¸o Unit 1: A day in the life of Period 4: Writing 1.Task 1: Read the passage and find all the verbs that are used in past simple and the connectors in the story Simple past Regular ... + hostesses were just beginning to serve lunch when plane began to shake + plane seemed to dip + people screamed in panic Unit 1: A day in the life of Period 4: Writing Task Task 2: The climax:...

Ngày tải lên: 18/09/2013, 05:10

... khác bổ sung -Nam thường có râu , quan sinh dục nam tạo tinh trùng - Nữ có kinh nguyệt , quan sinh dục nữ tạo trứng 4/ Củng cố dặn dò, nhận xét Rót kinh nghiƯm: ... HS nªu c©u cđa m×nh - GV nhËn xÐt HS kh¸c nhËn xÐt VD: BÐ Nga rÊt xinh x¾n víi chiÕc n¬ hång xinh xinh trªn ®Çu Nh÷ng ng«i nhµ xinh x¾n bªn hµng c©y xanh chóng em thi ®ua häc tËp Häc hµnh lµ nhiƯm ... vào tranh minh họa phóng to bảng Hoạt động 3: Hướng dẫn học sinh kể chuyện, trao đổi ý nghóa câu chuyện a Bài tập - GV: Dựa vào tranh minh họa trí nhớ, em tìm cho tranh 1- câu thuyết minh HĐHS...

Ngày tải lên: 19/09/2013, 14:10

... // define the variables String apples = "Purple pigs "; String oranges = "riding orange pumpkins"; String fruitBasket; %>

Ngày tải lên: 19/10/2013, 02:15

... vần mới: in, un – Ghi bảng 2.Hoạt động :Dạy vần: +Mục tiêu: nhận biết được :in, un, đèn pin, giun Phát âm ( em - đồng thanh) Phân tích ghép bìa +Cách tiến hành : cài: in a.Dạy vần : in Giống: ... diện vần : Vần in tạo bởi: i n Khác : in bắt đầu i GV đọc mẫu Đánh vần ( cá nhân - đ Hỏi: So sánh in an? thanh) Đọc trơn ( cá nhân - -Phát âm vần: -Đọc tiếng khoá từ khoá : pin, đèn pin đồng thanh) ... từ khoá : pin, đèn pin đồng thanh) Phân tích ghép bìa -Đọc lại sơ đồ: cài: pin in Đánh vần đọc trơn pin tiếng ,từ đèn pin ( cá nhân - đồng thanh) b.Dạy vần un: ( Qui trình tương tự) Đọc xuôi –...

Ngày tải lên: 28/10/2013, 17:15

... Stage / timing Prereading 5ms Activities warm-up GUESS THE ACTIVITIES * Instruction: -Divide class in to two teams: team A & team B -Show a video about linh’ daily routine in minute -ask ss ... the floor learning 5.phoning 6.cooking have lunch 8.take a nap 9.sulf the internet 10 watch TV 11 go to the bed * lead in: - It is Linh’sdaily routine -Do you have a daily routine? -is it same ... activities of Linh -2 ms for teams go to the board and write answers -team which more correct answer is winner * checking instruction (p.) *after ms T check answers and find the winner Arrangeme...

Ngày tải lên: 02/12/2013, 19:11