gene therapy and stem cells

... Arthritis Research & Therapy Vol 11 No Leandro leucocytes into inflamed joints), angiogenesis and tissue destruction [7] However, TNF and a related cytokine, lymphotoxin alfa ... of the spleen, particularly the compartmentalisation of B and T cells and the establishment of the marginal zone [8] In general, TNF and soluble LTα are thought to have overlapping roles in this ... trafficking into and retention of B cells in the synovia, thereby increasing their proportion in the peripheral blood However, both pre- and post-switch memory B cells can be found in the synovia and no...

Ngày tải lên: 09/08/2014, 14:22

... (columnar cells, goblet cells, enteroendocrine cells and Paneth cells) in mouse small intestine [28] The second school believes that the stem cells are sandwiched between the post-mitotic Paneth cells ... marks stem cells in the hair follicle [48] It is also suggested to mark stem cells in the stomach and mammary gland [34, 45] Like Lgr5, Ascl2 has also been identified to mark intestinal stem cells ... cells, including columnar cells, mucinsecreting cells, endocrine cells, and, in the small intestine, Paneth cells [8, 9] Other less common cell lineages are also present, such as caveolated cells...

Ngày tải lên: 11/09/2015, 10:04

... history and the most advanced discoveries in neural stem cells This book provides the strategies and challenges of utilizing neural stem cells for therapy of neurological disorders and brain and ... Formation of Nervous Systems and Neural Stem Cells in Ascidians 121 Kiyoshi Terakado Chapter Regeneration of Brain and Dopaminergic Neurons Utilizing Pluripotent Stem Cells: Lessons from Planarians ... orders@intechweb.org Neural Stem Cells and Therapy, Edited by Tao Sun p cm ISBN 978-953-307-958-5 Contents Preface IX Part Chapter Characterization of Neural Stem Cells Neural Stem Cells from Mammalian...

Ngày tải lên: 30/03/2014, 23:20

... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of genes ... hES cells and EB, node B contained the genes upregulated in hES cells only, node C contained the genes upregulated in EB only, and node D contained the genes that were upregulated in adult cells ... either hES cells or adult cells (Figure 6, panel B) For miR-106b, miR-92, miR-93, miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were...

Ngày tải lên: 18/06/2014, 15:20

... Ad.BMP-2 and Ad.BMP-4 generated approximately 30 to 60 ng/mL of gene product per 24 hours at day post-infection (Figures 1a, b) The amount of each transgene Figure Transgene expression and biochemical ... X and OP at day 3, IHH and ALP at day and RUNX2 at day 14 compared with Ad.GFP controls (Figure 7) These results suggest that BMP-2 and BMP-4 gene transfer induced a significant chondrogenic and ... cartilage and bone formation when delivered as recombinant protein [38] or via genetically modified cells [39] Notably, the latter study revealed differential effects on chondrogenesis and osteogenesis...

Ngày tải lên: 09/08/2014, 14:22

... attached cells stretched and took the shape of a typical spindle-shaped fibroblast phenotype These adherent cells could be readily expanded in vitro by successive cycles of trypsinization, seeding and ... density in ischemic limbs than cell therapy alone This may be partially due to the enhanced stem cells survival and greater differentiation rate of MSCs into vascular cells when administrated with ... expression and enchance cells survival Kortesidis and colleagues also demonstrated[31] that bone marrow derived-MSCs express factors that support cell survival and avoid apoptosis thereby preserving cells...

Ngày tải lên: 10/08/2014, 05:21

... somatic stem cells, using Oct4 gene ablation They show that Oct4 is dispensable for self-renewal in intestinal epithelium, bone marrow mesenchymal stem cells and HSC, hair follicle, brain and liver ... profiling of embryonic and adult stem cells Science 2002, 298:597-600 Liedtke S, Enczmann J, Waclawczyk S, Wernet P, Kögler G: Oct4 and its pseudogenes confuse stem cell research Cell Stem Cell 2007, ... observed in EC 2102Ep cells (+ control) All other cells expressed a variety of Oct4 pseudogene transcripts derived from chromosomes 1, 10 and 12 C-kit+ cells (T0) n = 7, c-kit+ cells (T7) n = 4,...

Ngày tải lên: 11/08/2014, 07:21

... derivation of human embryonic stem cells Stem cells 2005, 23:1228-1233 Moon SY, Park YB, Kim D-S, Oh SK, Kim D-W: Generation, culture, and differentiation of human embryonic stem cells for therapeutic ... progenitor cells are an essential ingredient for HIV gene therapy In view of the need for CD34 cells for HIV gene therapy as well as for other hematopoietic disorders, if one can produce these cells ... potential of embryonic stem cells Blood Rev 2005, 19:321-331 Menendez P, Wang L, Bhatia M: Genetic manipulation of human embryonic stem cells: A system to study early human development andpotential therapeutic...

Ngày tải lên: 13/08/2014, 09:21

... signature Stem cells Stem cells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature Tumour Birnie et al R83.3 Gleason included Benign Figure Distinctive stem cell and ... interactions and stem cell self-renewal Results Identification of gene products associated with a cancer stem cell phenotype By comparing RNA expression patterns from stem and committed cells, independent ... both stem and committed basal populations Mapping these 17 GO terms against our cancer stem cell signature identified 28 genes Searching these 28 genes against the Kyoto Encyclopedia of Genes and...

Ngày tải lên: 14/08/2014, 08:21

... gene therapy 21 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem ... are genetically identical cells that might avoid immune rejection 1.5 Stem cells as a vehicle for cancer gene therapy 1.5.1 Stem cell based cancer gene therapy Gene directed enzyme prodrug therapy ... al., 2001) Stem cells as delivery vehicles have been shown to enhance gene delivery efficiency (elaborated in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs),...

Ngày tải lên: 09/09/2015, 18:56

... adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have also been isolated from several other organs such as the brain (neuronal stem ... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... types and tissues including fat cells, cartilage, bone, tendons and ligaments, muscle cells, skin cells and even nerve cells (Short et al 2003) Neuronal stem cells The discovery of neuronal stem cells...

Ngày tải lên: 16/09/2015, 15:55

...   1.1   Stem cells   1.1.1   Human pluripotent stem cells   1.1.2   In vitro differentiation potential of human pluripotent and neural stem cells   1.2   Candidate ...   4.1   Genetic manipulations in human neuroepithelial-like stem cells for the generation of modified neuronal cultures 59   4.2   Generation of gene- corrected neural stem cells from ... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells...

Ngày tải lên: 26/11/2015, 09:54

... have transplanted the bone marrow stem cells into the necrotic femoral heads, and results show bone marrow stem cells can remove vascular lesions and promote angiogenesis in necrotic femoral heads, ... Induction of osteogenesis and adipogenesis of MSCs (×100) A: One week after osteogenesis induction, alkaline phosphatase staining showed positive cells B: One week after adipogenesis induction, ... [19-21] Recently, in order to improve the efficacy of stem cell transplantation, committed stem cells are isolated and purified, and single lineage stem cell transplantation is then performed Deng...

Ngày tải lên: 25/10/2012, 11:18

... (Lanes 1, and 3), BstXI (Lanes 4, 5, and 6), and BglII plus EcoRV (Lanes 7, 8, and 9) M, 1-kb DNA ladder Lanes 1, 4, and 7, 293A cells; Lanes 2, 5, and 8, ADrBMP6 DNA; Lanes 3, 6, and 9, ADhBMP6 ... E1 and E2b genes and the use of homologous (rat) BMP genes not improve the osteogenic potential of direct BMP adenovirus vector gene therapy, the results of this study indicate that BMP gene therapy ... BMP4 and BMP6 cDNAs were amplified, cloned, sequenced, and identified Recombinant adenoviruses encoding rat BMP4 and BMP6 were constructed and compared with ADhBMP4 and ADhBMP6 in the in vitro and...

Ngày tải lên: 31/10/2012, 17:08

... mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antifibrotic effect of transplanted mesenchymal stem ... presence and absence of IL-10 *P < 0.05 and **P < 0.01 versus Cont group (D) Representative western blots for collagen I and III in the presence and absence of 0.1 lM angiotensin II and IL-10 ... of the cells paracrine anti-fibrotic effects These findings help to improve our understanding of the cellular and molecular basis of MSCs’ anti-inflammatory and paracrine effects Materials and methods...

Ngày tải lên: 18/02/2014, 04:20

... stimulated cells in the absence of inhibitors Fig Effects of RhoA, Cdc42 and Rac1 on the expression of the CTGF/ CCN2 gene (A) Immunoblot analyses of RhoA, Cdc42 and Rac1 activation by S1P and FBS Cells ... anisomycin and FBS on actin stress fibers in SMCs and their modulation by toxin B and latrunculin B Cells were first stimulated with either S1P, anisomycin or FBS for 30 and then fixed, permeabilized and ... containing actinomycin D (10 lgÆmL)1) and the cells were harvested after 0, 0.5, 1, and h Total RNA was purified and analyzed by northern blot hybridization and phosphorimaging densitometry The...

Ngày tải lên: 19/02/2014, 16:20

... active gene promoters and to the LCR of the murine b-globin gene locus in MEL cells (A) Schematic representation of the murine b-globin gene locus LCR hypersensitive sites and globin genes are ... and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells ... the Rex-1 and b-actin genes but not the embryonic and adult globin genes (Fig 2) Upon differentiation the embryonic- and adult-specific b-globin genes are sequentially activated The ec -gene is activated...

Ngày tải lên: 07/03/2014, 12:20

... expression system is of interest for endogenous generation of siRNA and activation of the RNAi effect in human cells [45] Studies on an inhibition of BACE gene expression by endogenously generated ... cellular system and efficiently inhibited biosynthesis of b-secretase Fig Effect of ribozyme expression on BACE protein level in HEK293 cells The test cells were transfected with Rz-1 and Rz-2 encoding ... primers P1GAPDH and P2GAPDH was  15 times lower than primers P1BACE and P2BACE The HEK293 cells were transfected with plasmids encoding Rz-1, Rz-2 and with their inactive versions (Rz-1i and Rz-2i)...

Ngày tải lên: 08/03/2014, 08:20

... receptor tyrosine kinases Rse and Mer and their ligand Gas6 in testicular somatic cells J Androl 21, 291–302 Lu, Q., Gore, M., Zhang, Q., Camenisch, T., Boast, S., Casagranda, F., Lai, C., Skinner, ... ligand gene transcription in Sertoli cells is regulated by Sp1 J Biol Chem 274, 7756–7762 34 Lei, N & Heckert, L.L (2002) Sp1 and Egr1 regulate transcription of the Dmrt1 gene in Sertoli cells ... )194/)164 or )138/)119 (Sp1) and )30/)14 (E2F) of the mouse Mer promoter The two protein complexes identified are labelled a and b in (B), and a, b and c in (C) Lanes and show the results of binding...

Ngày tải lên: 08/03/2014, 23:20