gene therapy and cell based therapy

... regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell- based, in vitro and in silico experiments ... not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based and in silico analysis) will facilitate systematic understanding of the underlying ... l-glutamine and 5% fetal bovine serum) and maintained at 37 C and 5% CO2 For translocation experiments, cells were removed with 0.05% trypsin EDTA, and plated with cell solution of ã 106 cellsặmL)1...

Ngày tải lên: 16/03/2014, 11:20

... control and mutant DNAzyme-treated cells (Fig 5) Inhibition of Saos-2 cell invasion by Dz720 The plasminogen activator system can promote the breakdown of ECM and facilitate cell invasion and metastasis ... controls (P < 0.01) (Fig 6) Saos-2 cells treated with the active Dz720 had 42% and 17% fewer cells on the lower side of the filter compared with untreated control cells and cells treated with Dz720 mutant, ... uPA and uPAR to further inhibit cancer cell invasion and metastasis complex, the medium was replaced with DMEM containing 10% fetal bovine serum Cells were collected and assayed by northern and...

Ngày tải lên: 16/03/2014, 22:20

... stem cells in order to identify core ‘stem’ genes and to examine supportive tissue to understand what genes and pathways are involved not only in stem -cell differentiation, but also in stem -cell ... vivo and in vitro While skeletal muscle 39 Arthritis Research and Therapy Vol No Tuan et al itself contains stem cells known to be active in regeneration, these cells are distinct from MSCs and ... engineering and regeneration [134] Mesenchymal stem cells versus embryonic stem cells Embryonic stem (ES) cells are derived from the inner cell mass of the embryonic blastocyst These cells can...

Ngày tải lên: 09/08/2014, 01:21

... molecular and clinical data sets with a set of computational algorithms we were able to identify a gene- gene and gene- microRNA network that significantly stratifies patient prognosis By combining gene- expression ... Gene- expression and network -based analysis reveals a novel role for hsa-mir-9 and drug control over the p38 network in Glioblastoma Multiforme progression Rotem Ben-Hamo1 and Sol Efroni1,* ... of gene- expression and clinical information from 74 GBM patients (GEO accession: [GSE4412]) All patients were at grade III and IV, and ages varied from 18 to 82 years There were 46 females and...

Ngày tải lên: 11/08/2014, 12:21

... HBZ, p13, p30 and p12 interfere with cell cycling and/ or apoptosis [11-13] For example Tax, which is expressed by both infected CD4+ and CD8+ cells, can both stimulate cell cycling and block apoptosis ... MT2 [24] Cells were next cloned and cultured as PBMCs from HAM/ TSP, and all generated clones were assayed for HTLV-1 infection, tax expression, CD4+ and CD8+ expression, cell cycling and apoptosis, ... cellular morphological changes that depend on the T cell phenotype and tax expression [8,9] Cell morphology was therefore analyzed in all infected and uninfected clones and compared between cells...

Ngày tải lên: 12/08/2014, 23:23

... E2F gene family and a subunit encoded by the DP family of genes 43 In mammalian cells, there are six E2F genes (encoding E2F1, E2F2, E2F3, E2F4, E2F5 and E2F6) and two DP genes (encoding DP1 and ... target gene 87 FIGURE 18E: Fbxw7 is a novel E2F-responsive and TRIP-Brregulated gene 88 FIGURE 19A: Cell cycle analysis of U2OS cells at 48 hours postdecoy peptide treatment 92 FIGURE 19B: Cell ... GAL4-TRIP-Br1 and GAL4-TRIP-Br2 fusion proteins function as potent and dose-dependent stimulators of both basal and enhancer-activated transcription in oncoprotein-transformed cells (293 and COS cells)...

Ngày tải lên: 16/09/2015, 17:12

... neurodegeneration and provide a way to test gene therapy strategies for CNS disorders, injury, or aging 210 COMPONENTS OF CELL AND GENE THERAPY FOR NEUROLOGICAL DISORDERS NEUROTROPHIC FACTORS AND GENE ... primary cells, stem cells, and cell lines that withdraw from the cell cycle are NEUROTROPHIC FACTORS AND GENE THERAPY 215 now the focus of attention when considering the transplantation of cells ... OF CELL AND GENE THERAPY FOR NEUROLOGICAL DISORDERS CLINICAL TRIALS TESTING GENETICALLY MODIFIED CELLS AND NEUROTROPHIC FACTORS FOR NEURODEGENERATION Therapeutic options for human neurodegeneration...

Ngày tải lên: 10/04/2014, 22:11

... significant number of the apoptotic cells generated during extracorporeal photochemotherapy [15,16] While apoptotic cell instillation prevents and treats autoimmunity [8] and inflammation in several ... the efficiency of apoptotic cell infusion to modulate the arthritic response The feasibility of cellular therapy based on the immunomodulatory properties of apoptotic cells has already been evaluated ... lymph nodes were removed aseptically and single -cell suspensions were prepared Peripheral T cells were also analyzed after retro-orbital bleeding and red cell lysis with ACK lysing buffer (Biowhit-...

Ngày tải lên: 09/08/2014, 14:22

... proliferating cells (hematopoietic progenitors, epithelial cells and endothelial cells), all antigen-presenting cells (DCs, activated monocytes and activated B cells), CD8+ T cells and eosinophil ... aims to develop new NSC -based tumor-targeted gene therapy that improves the treatment of patients with breast cancer and overcomes the hurdles faced by stem cell- based gene therapy via two main ... Standardization of the large-scale mass production of cell therapeutics is a prerequisite for the widespread application of cell therapy Thus, standardization in generating human iPSC-derived cells...

Ngày tải lên: 09/09/2015, 10:06

... the stem cell vehicles to deliver immunogene products and the dendritic cell (DC) -based vaccination approach Targeted immuno -gene therapy approach using the stem cell delivery vehicle is based on ... delivery vehicle for cancer gene immunotherapy 1.2.1.1 Stem cell candidates for immunotherapy Stem cells are a population of cells that demonstrate self-renewal capacity and differentiation capability ... tumor cell- and DC -based, each offering unique advantages and disadvantages11 DC -based vaccines aside, all these approaches are based on the presumption that they can stimulate DCs and generate...

Ngày tải lên: 10/09/2015, 09:02

... (Lanes 1, and 3), BstXI (Lanes 4, 5, and 6), and BglII plus EcoRV (Lanes 7, 8, and 9) M, 1-kb DNA ladder Lanes 1, 4, and 7, 293A cells; Lanes 2, 5, and 8, ADrBMP6 DNA; Lanes 3, 6, and 9, ADhBMP6 ... E1 and E2b genes and the use of homologous (rat) BMP genes not improve the osteogenic potential of direct BMP adenovirus vector gene therapy, the results of this study indicate that BMP gene therapy ... BMP4 and BMP6 cDNAs were amplified, cloned, sequenced, and identified Recombinant adenoviruses encoding rat BMP4 and BMP6 were constructed and compared with ADhBMP4 and ADhBMP6 in the in vitro and...

Ngày tải lên: 31/10/2012, 17:08

... genes in the desired cell population In this chapter we explain the concept and aim of gene therapy, the different gene delivery systems and therapeutic strategies, how genes are deliv ered and ... Human Ge nome Project [1] have provided a genetic understanding of cellular processes and disease pathogenesis; numerous genes involved in disease and cellular processes have been identi fied ... experts in the gene therapy field Francisco Martớn Molina Principal Investigator Gene and Cell Therapy group Pfizer - Universidad de Granada - Junta de Andalucớa Centre for Genomics and Oncological...

Ngày tải lên: 17/03/2014, 21:20

... Dagmar Sigurdardottir and Barbara-ann Guinn Chapter 13 Targeting Intercellular Communication in Cancer Gene Therapy 327 Mohamed Amessou and Mustapha Kandouz Chapter 14 Cancer Gene Therapy with Small ... Introduction Gene therapy is a promising approach to treat intractable and refractory diseases at the genetic level Basically, in gene therapy, target gene expression is induced by delivering foreign genes ... perform gene therapy, that is, ex vivo methods and in vivo methods In ex vivo gene transfer, once cells are taken from a patient, in vitro gene transfer is performed, and then transfected cells...

Ngày tải lên: 23/03/2014, 14:20

... in the case of gene therapy as well Appropriate Candidates for Gene Therapy We now turn to another dimension of ethics issues in gene therapy, namely, who the candidates are for therapy We distinguish ... true in the case of Donald and the gene therapy clinical trial we are considering for his CF? 326 ETHICAL ISSUES IN MOLECULAR MEDICINE AND GENE THERAPY In the case of gene therapy there appear to ... The Lives to Come: The Genetic Revolution and Human Possibilities Simon and Schuster, New York, 1996 346 ETHICAL ISSUES IN MOLECULAR MEDICINE AND GENE THERAPY Kolata G Genetic defects detected...

Ngày tải lên: 10/04/2014, 22:10

... of some genes is up-regulated and of other genes down-regulated (Adapted from Anderson and Anderson, Electrophoresis, 1996.) MOLECULAR MEDICINE AND GENE THERAPY: AN INTRODUCTION gene and the ... transferred genes compromises both the clinical benefit and scientific value of gene therapy Gene Targeting Gene therapy approaches could be enhanced by directing gene transfer and expression to specific cells ... MOLECULAR MEDICINE AND GENE THERAPY: AN INTRODUCTION SUGGESTED READINGS Gene Therapy Anderson WF Human gene therapy Science 256:808–813, 1992 Dickson G (Ed.) Human Gene Therapeutics Chapman and Hall,...

Ngày tải lên: 10/04/2014, 22:10

... and Andreas Albers 27 Cancer Gene Therapy with Heat Shock Protein-65 Gene Katalin V Lukacs and Artit Nakakes 49 Recombinant Vaccinia Virus MVA for Generation and Analysis of T Cell ... Ohlmann, Volker Erfle, and Gerd Sutter 57 B: Suicide Gene Therapy Selection of Cytochrome P450 Genes for Use in Prodrug Activation -Based Cancer Gene Therapy Jodi E D Hecht and David J Waxman ... Evaluation of P450 Gene Therapy In Vivo Jodi E D Hecht, Pamela Schreiber Schwartz, and David J Waxman 107 vii viii Contents C: Anti-Oncogene and Suppressor Gene Therapy Intracellular Single-Chain...

Ngày tải lên: 11/04/2014, 09:42

... major band of closed circular DNA and minor amount (

Ngày tải lên: 11/04/2014, 09:59

... the Varicella zoster and Herpes simplex viral thymidine kinases in suicide gene therapy Cancer Gene Ther 7, 1456–1468 83 Kaneko, Y and Tsukamoto, A (1995) Gene therapy of hepatoma: bystander effect ... SK-BR-3, and Pancl cancer cells, transfection of CD gene in 10–20% of the cells is enough to kill the cell population In the same cells, transfection of bicistronic CD+UPRT gene in approx 1% of cells ... J and Niculescu-Duvaz, I (2000) Prodrug-activating systems in suicide gene therapy J Clin Investig 105, 1161–1167 19 Encell, L P., Landis, D M and Loeb, L A (1999) Improving enzymes for gene therapy...

Ngày tải lên: 11/04/2014, 10:33

... response by using syngeneic Ag-specific SV40-immortalized target cells, and either expanded CTLs or fresh peripheral blood mononuclear cells (PBMCs) as effector cells Methods Cells Specific-pathogen-free ... constructs and inhibit the E7-expressing TC-1 cell tumour growth in mice [60] These E6 and E7 genes, genetically modified and inserted into FPwt vectors, will be evaluated for safety, immunogenicity and ... syngeneic skin cells as targets and fresh PBMCs or expanded Ag-specific CTLs as effector cells CTLs were induced in all of the rabbits, but the ex-vivo cytolytic activity specific for E6 and...

Ngày tải lên: 18/06/2014, 16:20

... and troubles to be resolved and overcome, rAAV -based gene transfer vectors still represent one of the most promising gene therapy systems and gain increasing popularity In a search for "AAV gene ... interests Conclusion Progress in gene therapy is indisputable but has been slow and there have been many ups and downs The two major hurdles to gene therapy, safety and efficacy, remain roadblocks ... disorders Eur J Hum Genet 2004, 12:263-271 Pfeifer A, Verma IM: Gene therapy: promises and problems Annu Rev Genomics Hum Genet 2001, 2:177-211 Worgall S: A realistic chance for gene therapy in the...

Ngày tải lên: 18/06/2014, 18:20