gene symbol uqcrc2 cytochrome b c1 complex subunit 2 mitochondrial

... produces H2O2 but is not a result of SOD detoxification of O2•-) (Winterbourn, 20 08) Equations and show this 2O2•- + H+ O2•- + •NO O2•- + [4Fe-S ]2+ + 2H+ SOD H2O2 + O2 (Eq 1) ONOO- (Eq 2) [4Fe-S]3+ ... C57BL/6J and DBA/2J ‡ B6 -tm1Cje Sod2 mutant mouse originally produced in CD1 strain but backcrossed to C57BL/6 background for 13-14 generations # B6 -Sod2tm1Leb Sod2 mutant mouse produced on C57BL/6 ... (20 01); Pace et al (20 03); Velsor et al (20 04) Haasio et al (20 02a ,b, c) Bedoucha et al (20 01); Haskins et al (20 01); Tirmenstein et al (20 02) ; Narayanan et al (20 03); Shishido et al (20 03); Bova...

Ngày tải lên: 12/09/2015, 11:08

... (1990) OXBOX, a positive transcriptional element of the heart-skeletal muscle ADP/ATP translocator gene < /b> J Biol Chem 26 5, 20 585 20 588 Ó FEBS 20 04 40 12 A Talamillo et al (Eur J Biochem 27 1) 22 Suzuki, ... Scarpulla, R.C (20 02) Nuclear activators and coactivators in mammalian mitochondrial biogenesis Biochim Biophys Acta 1576, 1–14 16 Butow, R.A & Bahassi, E.M (1999) Orchestrating mitochondrial biogenesis ... several genes involved in mitochondrial biogenesis, such as OXBOX/REBOX [21 ], Mts [22 ] or GRBOX [23 ] However the putative transcription factors that recognize these DNA motifs remain to be identified...

Ngày tải lên: 07/03/2014, 16:20

... of both subunits in strains mutated in subunit e was checked by Western blot analyses of SDS-solubilized mitochondrial membranes Despite the presence of altered subunits e, the amount of subunit ... already observed for null mutants devoid of either subunit e or subunit g [14,15] It was previously shown that in the absence of subunit e, subunit g is not present in mitochondrial membranes [ 12] ... of subunit e, subunit g is not present in mitochondria whereas the absence of subunit g in the null mutant DATP20 does not preclude the presence of subunit e The lack of subunit g has also been...

Ngày tải lên: 31/03/2014, 01:20

... 1677. 928 1 1678. 028 3 67 81 LVLEVAQHLGENTVR 1 921 .9653 1 922 .0756 26 7 28 2 DQEGQDVLLFIDNIFR 22 52. 0686 22 52. 1958 29 7 317 IPSAVGYQPTLATDMGSMQER 75 Figure Amino acid sequence alignment between BP53 and ... 20 01, 28 6:7 -22 Chazal N, Gerlier D: Virus entry, assembly, budding, membrane rafts Microbiology and Molecular Biology Reviews 20 03, 67 :22 6 -23 7 Villanueva RA, Rouillé Y, Dubuisson J: Interactions between ... could be inhibited by rBP53, and that the inhibition was dose dependent (Fig 3) No competitive binding was observed between BSA or PBS and WSSV Here results showed that the binding between rBP53...

Ngày tải lên: 12/08/2014, 04:20

... daN (24 ,27 ), dad (24 ,28 ), daN (25 ,29 ), dab (25 ,28 ), dad (25 ,28 ) and daN (26 ,30) The interruption between helix I and helix II is further confirmed Structural studies of membrane proteins by NMR in solution ... Direct observation Science 28 6, 1 722 –1 724 Panke, O., Gumbiowski, K., Junge, W & Engelbrecht, S (20 00) ¨ F-ATPase: specific observation of the rotating c subunit oligomer of EF0EF1 FEBS Lett 4 72, 34–38 ... of the transmembrane 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 H+-transporting subunit c of the F1F0 ATP synthase Biochemistry 37, 8817–8 824 Matthey, U., Kaim, G., Braun, D., Wuthrich,...

Ngày tải lên: 08/03/2014, 10:20

... aN214C ⁄ cM65C aN214C ⁄ cI66C aA217C ⁄ cM65C aA217C ⁄ cI66C aI 221 C ⁄ cG69C aI 223 C ⁄ cL72C aI 223 C ⁄ cY73C aL 224 C ⁄ cL72C aL 224 C ⁄ cY73C aI 225 C ⁄ cL72C aI 225 C ⁄ cY73C ++++ +++ +++ +++ ± +++ +++ + +++ ... (%) aI 223 C ⁄ cV58C aI 223 C ⁄ cL59C aN230C ⁄ cS66C aN230C ⁄ cT67C aN230C ⁄ cG68C aN230C ⁄ cI69C aN230C ⁄ cY70C aA233C ⁄ cI69C aA233C ⁄ cY70C aI237C ⁄ cV73C aG239C ⁄ cL76C aG239C ⁄ cI77C aL240C ⁄ ... numbering) Cys pair (E c numbering) aI 223 C ⁄ cV58C aI 223 C ⁄ cL59C aN230C ⁄ cS66C aN230C ⁄ cT67C aN230C ⁄ cG68C aN230C ⁄ cI69C aN230C ⁄ cY70C aA233C ⁄ cI69C aA233C ⁄ cY70C aI237C ⁄ cV73C aG239C...

Ngày tải lên: 16/03/2014, 06:20

... c subunit is assembled with the a 3b3 core [25 , 26 ] The hybrid enzyme from the a ,b subunits of a thermophilic bacterium and the mutant CF1 c subunit (D194 23 0) was insensitive to added e subunit ... demonstrated by fluorescence mapping [20 ] and crosslinking [21 ] that the e subunit is in close proximity to the c subunit The e subunit interacts directly with the c subunit [22 24 ], but does so ... by chloroplast and cyanobacterial F1-ATPase subunits Biochim Biophys Acta 1144, 27 8 28 4 32 Ni ZL, Wang DF & Wei JM (20 02) Substitutions of the conserved Thr 42 increased the roles of the e subunit...

Ngày tải lên: 23/03/2014, 13:20

... M, Borsch M, Zimmermann B, Turina P, Dunn SD & Graber P (20 04) Binding of the b- subunit in the ATP synthase from Escherichia coli Biochemistry 43, 10541064 FEBS Journal 27 5 (20 08) 485048 62 ê 20 08 ... catalysis J Biol Chem 27 3, 22 2 922 229 7 29 Watts SD, Tang C & Capaldi RA (1996) The stalk region of the Escherichia coli ATP synthase Tyrosine 20 5 of the c subunit is in the interface between the ... of the enzyme Biophys J 81, 122 0 123 3 13 Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M & Greie JC (20 05) Both rotor and stator subunits are necessary for efcient binding of F1 to...

Ngày tải lên: 24/03/2014, 00:21

... titration of subunit H to the catalytic A subunit showed that subunit H binds in a saturable fashion to subunit A with a Kd of 20 6 nm [ 12] Determination of the shape of this hydrated subunit H in ... circular dichroism Anal Biochem 20 9, 32 44 23 Provencher SW (19 82) A constrained regularization method of inverting data represented by linear algebraic 18 12 24 25 26 27 28 29 30 or integral quations ... formation between subunits A and H, the close proximity of subunit H via its N-terminus to subunit E, and the proximity of subunits D and E leads us to speculate whether both subunits H and E might be...

Ngày tải lên: 30/03/2014, 04:20

... Baines CP, Barton ER, Vuagniaux G, Sweeney HL, Robbins J & FEBS Journal 27 8 (20 11) 11 121 125 ê 20 11 The Authors Journal compilation ê 20 11 FEBS C Chinopoulos et al 15 16 17 18 19 20 21 22 23 24 ... essential for inhibition of the mitochondrial permeability transition pore by cyclosporin A and by cyclophilin D ablation J Biol Chem 28 3, 26 30 726 311 Lin DT & Lechleiter JD (20 02) Mitochondrial targeted ... centrifugation at 20 00 g for min, the beads were washed twice for in a solution FEBS Journal 27 8 (20 11) 11 121 125 ê 20 11 The Authors Journal compilation ê 20 11 FEBS 1 121 Effect of CYPD on mitochondrial...

Ngày tải lên: 14/02/2014, 19:20

... conditions (B) , the c ring is fragmented, which leads to two series of subcomplexes containing only c2 ⁄ subunits (indicated by blue vertical bars) or one c1 subunit and 1–9 c2 ⁄ subunits (indicated by ... 18 19 20 21 22 23 24 25 26 encode proteolipids of 8- and 18-kDa J Biol Chem 27 4, 33999–34004 Aufurth S, Schagger H & Muller V (20 00) Identification ¨ ¨ of subunits a, b, and c1 from Acetobacterium ... (lanes and 8) or subunit c2 ⁄ (lane 9) The antibody against c2 ⁄ also reacts with c1 20 00 FEBS Journal 27 5 (20 08) 1999 20 07 ª 20 08 The Authors Journal compilation ª 20 08 FEBS Hybrid V0–F0 rotor...

Ngày tải lên: 18/02/2014, 17:20

... phospholipase A2 J Biol Chem 26 6, 722 7– 723 2 Ó FEBS 20 04 Regulation of iPLA2c biosynthesis (Eur J Biochem 27 1) 4 723 13 Zupan, L.A., Weiss, R.H., Hazen, S.L., Parnas, B. L., Aston, K.W., Lennon, ... motifs J Biol Chem 27 2, 8567–8575 25 Stewart, A., Ghosh, M., Spencer, D.M & Leslie, C.C (20 02) Enzymatic properties of human cytosolic phospholipase A (2) gamma J Biol Chem 27 7, 29 526 29 536 26 Mancuso, ... GenBank accession number AF263613) located on the long arm of chromosome at 118 cM [26 ] Like other members of the iPLA2 family – iPLA2a (patatin, found in potato tubers) [28 ] and iPLA 2b [23 ]...

Ngày tải lên: 19/02/2014, 16:20

... direct observation Science 28 6, 1 722 –1 724 19 92 P Turina and B A Melandri (Eur J Biochem 26 9) Ó FEBS 20 02 18 Panke, O., Gumbiowski, K., Junge, W & Engelbrecht, S (20 00) ¨ F-ATPase: specific observation ... glycine 21 8 and histidine 24 5 in the alpha subunit of F1F0 ATP synthase in Escherichia coli J Biol Chem 26 9, 323 13– 323 17 25 Cain, B. D & Simoni, R.D (1988) Interaction between Glu -21 9 and His -24 5 ... Escherichia coli F0F1-ATP synthase J Biol Chem 27 2, 326 35– 326 41 29 Hatch, L.P., Cox, G .B & Howitt, S.M (1998) Glutamate residues at positions 21 9 and 25 2 in the a -subunit of the Escherichia coli ATP...

Ngày tải lên: 21/02/2014, 03:20

... could probably not be observed on the SDS gel because the detergent dissociates the less stable c2 to c10 assemblies into monomeric units FEBS Journal 27 2 (20 05) 5474–5483 ª 20 05 FEBS Therefore, ... Dimroth P (20 02) Intersubunit bridging by sodium ions as rationale for the unusual stability of the FEBS Journal 27 2 (20 05) 5474–5483 ª 20 05 FEBS Structural evidence for a constant c11 ring stoichiometry ... stoichiometry 23 24 25 26 27 28 29 30 turbines of Na+-F1Fo ATP synthases EMBO Rep 3, 1094–1098 Van der Laan M, Bechtluft P, Kol S, Nouwen N & Driessen AJ (20 04) F1F0 ATP synthase subunit c is a substrate...

Ngày tải lên: 07/03/2014, 21:20

... into liposomes Eur J Biochem 22 5, 167–1 72 Masaike T, Suzuki T, Tsunoda SP, Konno H & Yoshida M (20 06) Probing conformations of the b subunit 26 54 23 24 25 26 27 28 29 30 31 32 33 of F0F1-ATP synthase ... reconstituted FEBS Journal 27 8 (20 11) 26 47 26 54 ª 20 11 The Authors Journal compilation ª 20 11 FEBS 26 53 ATP synthesis by thermophilic ATP synthase 13 14 15 16 17 18 19 20 21 22 N Soga et al purple membrane ... synthesis by ATP bound to a noncatalytic binding site J Biol Chem 27 0, 21 571 21 578 Bald D, Amano T, Muneyuki E, Pitard B, Rigaud JL, Kruip J, Hisabori T, Yoshidai M & Shibata M (1998) ATP synthesis by...

Ngày tải lên: 22/03/2014, 16:20

... FEBS Journal 27 7 (20 10) 21 92 22 05 Journal compilation ª 20 10 FEBS No claim to original US government works 22 03 PEDF binding to F1-ATP synthase 22 23 24 25 26 27 28 29 30 31 32 L Notari et al blastoma ... (uniprotkb:P21306), F-ATPase delta subunit (uniprotkb:Q 121 65), F-ATPase gamma subunit (uniprotkb:P38077), F-ATPase beta subunit (uniprotkb:P00830) and F-ATPase alpha subunit (uniprotkb:P0 725 1) by surface ... F-ATPase epsilon subunit (uniprotkb:P21306), F-ATPase delta subunit (uniprotkb:Q 121 65), PEDF (uniprotkb:P36955), F-ATPase alpha subunit (uniprotkb:P0 725 1), F-ATPase beta subunit (uniprotkb:P00830) and...

Ngày tải lên: 22/03/2014, 21:21

... SDS/PAGE Subunit b was subsequently identified by N-terminal sequencing and subunit a was identified by immuno-blotting with an antibody directed against subunit a (Fig 1) Maximal yield of subunit b was ... Western blot was developed using an antibody directed against subunit a 25 70 F Wehrle et al (Eur J Biochem 26 9) Fig Purity of individual Fo subunits estimated by SDS/PAGE Subunits a, b, or c ... analysed by SDS/ PAGE ( 12% acrylamide) [23 ] Lane 1: subunit a with N-terminal His10 tag (33.6 kDa); lane 2: monomeric subunit c (8.7 kDa); lane 5: subunit b with C-terminal His6 tag (20 kDa);...

Ngày tải lên: 31/03/2014, 15:20

... ATP5AL1 ATP 5B, ATP5BL1 ATP5C2, ATP5D, ATP5E, ATP5F1, ATP5G1, ATP5G2, ATP5G3, ATP5H, ATP5HP1, ATP5I, ATP5J, ATP5J2, ATP5L, ATP5L2, ATP5O, ATP5S ATP6, ATP6AP1, ATP6AP2 ATPSBL1, ATPSBL2 MT-ATP6, ... phân tử MRC Cambridge MRC Mitochondrial Biology Unit (cũng Cambridge) tinh chế miền xúc tác F1 ATP synthase Cấu trúc không đối xứng lớn protein biết đến, biểu thị mô hình xúc tác quay Boyer xác định ... tử, protein quang hợp ATP synthase (ở b ) Các ATP synthase b cô lập từ ti thể tim b (Bos taurus) mặt hóa sinh cấu trúc, tính đặc trưng ATP synthase Tim b sử dụng nguồn cho enzim nồng độ cao...

Ngày tải lên: 18/07/2014, 14:00

... macromolecules can be measured in physiological/pathological processes by mitochondrial matrix subunit c matrix half-channel H+ mitochondrial inner membrane Arg210 Asp61 subunit a H+ H+ H+ H+ ... and macromolecules by stimulating autophagy-like mechanisms http://www.tbiomed.com/content/4/1/9 20 21 22 strate-modulated reactions of putidamonooxin Eur J Biochem 20 00, 26 7:5 926 -5934 Villaverde ... kinetics of beef heart mitochondrial ATPase Arch Biochem Biophys 1984, 23 1:498-5 02 Csaszar AG, Czako G, Furtenbacher T, Tennyson J, Szalay V, Shirin SV, Zobov NF, Polyansky OL: On equilibrium structures...

Ngày tải lên: 13/08/2014, 16:21

... Inhibition Assay 25 2. 6 SDS PAGE 26 2. 7 Protein Quantitation Assays 26 2. 7.1 Lowry-Peterson Method 26 2. 7 .2 Biocinchoninic Acid (BCA) Protein Assay 27 2. 7.3 Photometric 28 0 nm Absorption 27 Chapter ... bromide) (DPX) Quenching Optimization 21 2. 4 BR-liposome Proton Pumping under Light Illumination 22 2. 5 TF1Fo ATP Synthase 22 2. 5.1 Culture of Bacillus PS3 22 2. 5 .2 Cholate Treatment and n-dodecyl ... Methods 17 2. 1 Halobacterium Salinarum 18 2. 1.1 Cell Culture 18 2. 1 .2 Extraction and Purification of Bacteriorhodopsin 19 2. 1.3 Sucrose Density Gradient 19 ii 2. 2 20 2. 2.1 Incorporation of BR into...

Ngày tải lên: 15/09/2015, 22:43