gene symbol slc25a13 calcium binding mitochondrial carrier protein aralar2

... the hydrophilic proteins (for example, such as the mitochondrial matrix proteins, with respect to this study), membrane proteins are poorly resolved by the fact that many of these proteins precipitate ... (ROS /RNS) in the pathogenesis of both rare and common human diseases (Droge, 2002) Mitochondrial diseases due to mutations in nDNA and mtDNA encoding for mitochondrial proteins are complex, and ... estradiol regulate mitochondrial function (Nilsen & Diaz Brinton, 2003, Stirone et al., 2005), increased expression of mitochondrial proteins (Nilsen et al., 2007) and enhanced mitochondrial antioxidant...

Ngày tải lên: 12/09/2015, 11:08

... other by mAu Ó FEBS 2003 Actin and small heat shock protein (Eur J Biochem 270) 895 Fig Fluorescence spectra of 140 lM ANS in the absence of added proteins (1) and in the presence of 2.25 lM of intact ... thermally inactivated protein In this state, the environment of W residues remains comparatively hydrophobic, actin weakly interacts with ANS and only a small portion of protein forms high molecular ... are smaller than the size of symbol (B) Influence of HSP25 and its 3D mutant on the extent of actin polymerization Samples containing lM of actin (10% of pyrene-labeled protein) were preincubated...

Ngày tải lên: 20/02/2014, 23:20

... able to uptake plasmid DNA by a mechanism that involves macropinocytosis and binding to two DNA -binding cell surface proteins, ezrin and moesin [28] In addition, some specific receptors, such as ... after gene gun immunization J Exp Med 1998, 188:1075-1082 Ulmer JB, Deck RR, Dewitt CM, Donnhly JI, Liu MA: Generation of MHC class I-restricted cytotoxic T lymphocytes by expression of a viral protein ... A, Baer A, Hengge UR: Uptake and trafficking of DNA in keratinocytes: evidence for DNA -binding proteins Gene Ther 2004, 11:765-774 Jozefowski S, Sulahian TH, Arredouani M, Kobzik L: Role of scavenger...

Ngày tải lên: 14/08/2014, 19:22

... Genetic Vaccines and Therapy 2009, 7:11 Background Protein- calorie malnutrition (PCM) is still the most common type of undernutrition ... represent a promising new approach to vaccination in which the gene for a foreign antigen is expressed within the host's cells These vaccines generated humoral and cell-mediated immune responses followed ... [15] Our group has been working with DNA vaccines constructed by inserting the heat shock protein 65 gene from Mycobacterium leprae (hsp65) into plasmid vectors (DNAhsp65) Theoretically, this...

Ngày tải lên: 14/08/2014, 19:22

... through a wide range of mechanisms Nucleolin is a major nucleolar phosphoprotein that belongs to a large family of RNA -binding proteins [26] It plays a role in the pre-rRNA transcription Caspase-3 ... Bcl-2 protein expression and mRNA stability in the presence or absence of H2O2 (A) Western blot analyses showing the effect of nucleolin ⁄ C23 on Bcl-2 protein expression Total cellular proteins ... preribosomal ribonucleoprotein assembly and processing [27] Nucleolin has also been suggested to regulate cell proliferation and growth, cytokinesis, replication, embryogenesis and nucleogenesis [28] by...

Ngày tải lên: 16/02/2014, 09:20

... Tat fusion proteins by protein transduction technology Several small regions of proteins, called protein transduction domains, have been developed to allow the delivery of exogenous protein into ... include carrier peptides derived from the HIV-1 Tat protein, Drosophila Antennapedia (Antp) protein and herpes simplex virus VP22 protein [17] To increase the biological activity of transduced proteins ... transduction of proteins in their native condition To express the cell-permeable PEP-1–HSP27 protein, the human HSP27 gene was fused to a PEP-1 peptide in a bacterial expression vector to produce a genetic...

Ngày tải lên: 18/02/2014, 17:20

... shock proteins (sHsp) and their interaction with cytoskeleton proteins Biochemistry (Mosc) 67, 511–519 Macario AJL (1995) Heat-shock proteins and molecular chaperones: implications for pathogenesis, ... target protein sequestration The decrease in polarity of the C-terminal extension may also facilitate interaction between the extension and hydrophobic chaperone binding sites, resulting in the binding ... sites, resulting in the binding sites being less accessible to the target protein, leading to a decrease in target protein binding [26] In summary, the three negatively charged glutamic acid residues...

Ngày tải lên: 07/03/2014, 04:20

... p26 cDNAs p26 amino acid substitutions were generated by site-directed mutagenesis by using the QuikChangetm Site-directed Mutagenesis kit (Stratagene, La Jolla, CA, USA), using pRSET.C-p26–3-6-3 ... heat stress proteins and a new family of proteins containing a-crystallin domains (Acd proteins) Cell Stress Chaperones 6, 225–237 Narberhaus F (2002) a-crystallin-type heat shock proteins: socializing ... Table Primers for site-directed mutagenesis of p26 Single amino acid substitutions were generated within the p26 a-crystallin domain by site-directed mutagenesis using primers presented as sense...

Ngày tải lên: 07/03/2014, 12:20

... major heat shock genes is significantly lowered by BA treatment [12,13] Whereas BA stress activated the entire set of heat shock genes when the solubility of the most aggregation-prone protein homoserine ... cytosolic calcium and mitochondrial membrane potential in cultured rabbit corneal epithelial cells Toxicol Appl Pharmacol 129, 23–35 20 Qian L, Song X, Ren H, Gong J & Cheng S (2004) Mitochondrial ... causing measurable protein denaturation We also demonstrate that, just as in response to heat treatment, there are immediate increases in intracellular free Ca2+ level and mitochondrial membrane...

Ngày tải lên: 07/03/2014, 12:20

... concentration of protein (curve on Fig 2A) In the second, the column was loaded with the same volume of 0.3 mgÆmL)1 protein (curve on Fig 2A) In the third, the column was loaded with 30 lL of protein ... outer dense fiber protein is the 10th member of the superfamily of mammalian small stress proteins Cell Stress Chaperones 8, 62–69 Clark, J.I & Muchowski, P.J (2000) Small heat-shock proteins and ... shock-related protein- 20 is an actin-associated protein J Vasc Surg 29, 326–333 36 Buchner, J., Ehrnsperger, M., Gaestel, M & Walke, S (1998) Purification and characterization of small heat shock proteins...

Ngày tải lên: 07/03/2014, 15:20

... different fractions was determined by immunoblot analysis Mitochondrial protein cytochrome oxidase subunit II was used as a marker of mitochondrial protein and Ponceau S staining was used as the loading ... interaction between HSP70 and Smac proteins, and the ATP -binding domain of HSP70, rather than the peptide -binding domain, was essential for this specific function of the protein Recent studies have revealed ... described in the Experimental procedures Protein protein contents were determined by the Bradford assay (Bio-Rad, Hercules, CA, USA), and equal amounts of proteins (10–20 lg) were loaded in each...

Ngày tải lên: 16/03/2014, 01:20

... heat shock proteins (sHSPs) play an important role in this process sHSPs comprise a large and diverse family of proteins with molecular masses from 12 to 43 kDa The members of this protein family ... agrees with previous studies on the sHSP complexes with various denatured proteins, suggesting a maximum binding capacity of one protein subunit per one sHSP subunit [37] Under these conditions, the ... enzymes [37,42,43] Generally, sHSPs cannot protect target proteins from denaturation and cannot refold denatured substrate However, sHSPs prevent the aggregation of denatured target proteins, forming...

Ngày tải lên: 16/03/2014, 06:20

... GST, S pombe whole cell protein extracts and GST-Cdc37 The antibody recognized GST-Cdc37 and a 64 kDa protein from S pombe protein extracts (Fig 2A) To verify that the 64 kDa protein is indeed Cdc37, ... domain alone can sustain cellular viability These truncated proteins not contain the postulated Hsp90 -binding domain, suggesting that binding of the cochaperone Hsp90 by Cdc37 is not required for ... although the human protein may act inefficiently in S pombe blot analysis on GST, S pombe protein extracts and GST-Cdc37 using depleted serum results in a loss of signal against S pombe protein extracts...

Ngày tải lên: 16/03/2014, 22:20

... shock protein, alphaB-crystallin Biochemistry 43, 15785–15795 20 Benndorf R & Welsh MJ (2004) Shocking degeneration Nat Genet 36, 547–548 21 Sun Y & MacRae TH (2005) The small heat shock proteins ... increased after its binding to hydrophobic sites on the protein molecules [24,29] Titration of HSP22 with bis-ANS was accompanied by an increase in fluorescence at 495 nm, indicating binding of the ... model protein substrates (A) Reduction induced aggregation of insulin (0.2 mgÆmL)1) in the absence of HSP22 (curve 0) or in the presence of 0.1 mgÆmL)1 (empty symbols) or 0.2 mgÆmL)1 (filled symbols)...

Ngày tải lên: 23/03/2014, 07:20

... because the localization of Sm proteins at SC35 speckles is more RNA-dependent than the Sm proteins that are diffusely distributed throughout the nucleoplasm No Sm protein could be detected in ... specific proteins [25] Unfortunately, the identity of this ubiquitinated protein remains to be established However, a role for phosphorylated aB-crystallin in degradation of a speckle protein ... with a SC35 specklespecific protein The additional recruitment of FBX4 may stimulate the ubiquitination of the speckle protein Acknowledgements We thank Dr G Eguchi for his generous gift of the anti-(aB-crystallin)...

Ngày tải lên: 23/03/2014, 13:20

... protein Sec63p was shown to be an abundant protein in canine pancreatic microsomes and was found in association with the Sec61 complex [19–21] The Sec63-related protein ER-resident J-domain protein ... Saccharomyces cerevisiae, four Hsp40 proteins with a luminal J-domain have been identified: the two membrane proteins Sec63p [5] and Scj2p [26], and the two luminal proteins Scj1p [27] and Jem1p [28] ... luminal protein that is involved in the completion of protein translocation [14,15] Furthermore, BiP was shown to seal the luminal end of the mammalian Sec61 complex in the absence of protein...

Ngày tải lên: 30/03/2014, 03:20

... quantitation of microgram quantities of proteins utilizing the principle of protein dye binding Anal Biochem 72, 248–254 40 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the ... kDa heat shock protein Mol Pharmacol 65, 407– 415 Song Y, Zweier JL & Xia Y (2001) Heat-shock protein augments neuronal nitric oxide synthase activity by enhancing Ca2+ ⁄ calmodulin binding Biochem ... rats treated with HSD and C.I.1 Aliquots of brain soluble material (20 lg protein) and of aorta total lysate (50 lg protein) , obtained as described in Experimental procedures, from untreated...

Ngày tải lên: 30/03/2014, 04:20

... procedures Site-directed mutagenesis of p26 cDNA Internal deletions of p26 were generated by site-directed mutagenesis with the QuikChangeTM Site-directed Mutagenesis kit (Stratagene, La Jolla, CA, USA), ... MacRae Small heat shock protein sequence motifs Table Primers for site-directed mutagenesis of p26 Internal deletions of p26 cDNA were generated by site-directed mutagenesis using the listed ... shock protein 70 systems to reactivate a heat-denatured protein Plant Physiol 122, 189– 197 24 Basha E, Lee GJ, Breci LA, Hausrath AC, Buan NR, Giese KC & Vierling E (2004) The identity of proteins...

Ngày tải lên: 30/03/2014, 20:20

... shock/chaperone protein- mediated tumor rejection Curr Opin Immunol 2003, 15:89-94 24 Flynn GC, Chappell TG, Rothman JE: Peptide binding and release by proteins implicated as catalysts of protein assembly ... shock proteins transfer peptides during antigen processing and CTL priming Immunogenetics 1994, 39:93-98 Suto R; Srivastava PK: A mechanism for the specific immunogenicity of heat shock protein- chaperoned ... model, HSP96 and MHC have homology in the peptide -binding domains [3] Many peptides being evaluated in melanoma immunotherapy trials are restricted in binding to a specific human MHC haplotype (human...

Ngày tải lên: 18/06/2014, 16:20

... to the recipient vessels [14] Heat shock proteins (HSP) HSP are found in all organisms and all cell types They are the most phylogenetically conserved proteins known with respect to both structure ... of heat shock genes Science 1993, 259:1409-10 Tavassol F, Starke OF, Kokemüller H, Wegener G, Müller-Tavassol CC, Gellrich NC, Eckardt A: Prognostic significance of heat shock protein 70 (HSP70) ... stress proteins in vitro [27] Hurwitz et al (2010) could show that radiation therapy induces expression of HSP70 in patients with prostate cancer [28] Furthermore, the expression of heat shock proteins...

Ngày tải lên: 09/08/2014, 09:20