gene structure and gene regulation

... in the ORF3 and E genes (43%) and the lowest C-count in the 1a/1b genes and the 3' UTR (13%, 14% and 14%, respectively) The N gene is most moderate in its nucleotide bias, with 21% C and 32% U, ... polyprotein genes and the structural genes The cumulative GC-skews for HCoVNL63 and four other coronaviruses: HCoV-OC43, HCoV229E, PEDV and SARS-CoV are presented in Figure In the 1a/1b genes, the ... content of individual HCoV-NL63 genes and the Nucleotide content of individual HCoV-NL63 genes and the 5'/3' untranslated regions (UTR) the nucleotide composition and its influence on the codon...

Ngày tải lên: 18/06/2014, 22:20

... in the ORF3 and E genes (43%) and the lowest C-count in the 1a/1b genes and the 3' UTR (13%, 14% and 14%, respectively) The N gene is most moderate in its nucleotide bias, with 21% C and 32% U, ... polyprotein genes and the structural genes The cumulative GC-skews for HCoVNL63 and four other coronaviruses: HCoV-OC43, HCoV229E, PEDV and SARS-CoV are presented in Figure In the 1a/1b genes, the ... content of individual HCoV-NL63 genes and the Nucleotide content of individual HCoV-NL63 genes and the 5'/3' untranslated regions (UTR) the nucleotide composition and its influence on the codon...

Ngày tải lên: 20/06/2014, 04:20

... at 50 °C and 10 at 95 °C, followed by a total of 40 twotemperature cycles (15 s at 95 °C and at 60 °C) For the generation of standard curves, serial dilutions of a cDNA sample were used and mRNA ... and 3) Translational regulation of iPLA2c in myocardium Owing to the obvious complexity of the regulation of iPLA2c resulting from the combined presence of transcriptional and translational regulation, ... iPLA2c message, protein mass and activity increased during adipogenesis and siRNA knockdown of iPLA2c-inhibited adipogenesis [67] Similarly, we demonstrated the dramatic up -regulation of the iPLA2c...

Ngày tải lên: 19/02/2014, 16:20

... Discussion From its identification as a Drosophila gene responsible for resistance to morphogenetic and toxic effects of JH and JHAs, the Met gene product has been implicated to have an involvement ... activator of gene regulation [45] Contrary to this, MET exists in the nucleus even in the absence of ligand Upon ligand binding, it becomes a transcriptional activator This resembles the ligand-dependent ... purified, digested and subcloned into EcoRI and XbaI sites FEBS Journal 272 (2005) 1169–1178 ª 2005 FEBS Characterization of Drosophila Met gene product of pBluescript II (Stratagene), and the identity...

Ngày tải lên: 16/03/2014, 18:20

... sensitive, and that the pA1lacO-1 promoter and the lacI q1 gene are surrounded by a DNA fragment originating from upstream the gyrA gene and a fragment containing the N-terminal part of the gyrA gene ... digested with the enzymes DraI and EcoRV and a 842-bp fragment containing the DNA region upstream of topA and the topA promoter was isolated and inserted into pUC19 (New England Biolabs) digested with ... were obtained from and used as recommended by New England Biolabs and Boehringer Mannheim Plasmid and ATP, ADP extraction Aliquots (0.8 mL) were removed from cell cultures and placed into an...

Ngày tải lên: 18/03/2014, 01:20

... cycle, including positive-strand and minus-strand DNA synthesis, pre-genomic RNA packaging, and virion formation and release [17,18] This core protein is a phosphoprotein, and its function may be ... proteins, and thereby may influence host or viral gene expression at the post-transcriptional level In this review, we describe these viral SR proteins and also discuss the interplay between host and ... protein–protein and protein–RNA interactions of SR proteins [1–5] Reversible phosphorylation of SR proteins is important for assembly and function of the spliceosome and for proper regulation of...

Ngày tải lên: 23/03/2014, 06:20

... FEBS T N Rampias et al Genomic structure and expression analysis of the Cc RNase epidermis and intestine of 6-day-old larvae and adult heads and ovaries, was blotted and hybridized using the Cc ... of these have been purified and characterized as alkaline [12] and acidic RNases [13], and a third poly(U)- and poly(C)-specific ribonuclease has also been isolated and characterized [14,15] In ... profile of the RNase j gene at various developmental stages and in several tissues of the insect C capitata We have also determined the general genomic organization of this gene and analyzed its structural...

Ngày tải lên: 23/03/2014, 06:20

... known Fig Structures of tetracyclines used in eukaryotic gene regulation (A) Structure of tetracycline with the pKa values of the three titratable groups (B) Structure of doxycycline (C) Structure ... mammalian gene regulation concepts on functional genomic research, metabolic engineering, and advanced gene therapies Biotechnol Prog 17, 1–51 48 Lewandoski, M (2001) Conditional control of gene expression ... Varmus, H.E (2001) Induction and apoptotic regression of lung adenocarcinomas by regulation of a K-Ras transgene in the presence and absence of tumor suppressor genes Genes Dev 15, 3249–3262 Moody,...

Ngày tải lên: 23/03/2014, 21:20

... Extensive and divergent circadian gene expression in liver and heart Nature 417, 78–83 16 Rudic RD, McNamara P, Curtis AM, Boston RC, Panda S, Hogenesch JB & Fitzgerald GA (2004) BMAL1 and CLOCK, ... that gene expression follows the metabolic rhythm [21,40] Almost all genes are cyclically transcribed and can be clustered in three groups: 650 are expressed in the oxidative phase, and 2429 and ... sulfur metabolism and RNA metabolism occur in the oxidative phase; cell division and mitochondrial biogenesis occur in the first Metabolic cycles reductive phase and glycolysis and fatty acid oxidation...

Ngày tải lên: 30/03/2014, 04:20

... questions of gene regulation and metabolism without explicit mention of the connection between networks and the phenotype One of the challenges, computationally, in understanding gene regulation ... approaches to metabolism and gene regulation This is an area of computational biology that welcomes new methods, ideas, and approaches with the goal of generating a better understanding of the complex ... Evgenia V Kriventseva, and Nicola Mulder vii 17 19 Information Fusion and Metabolic Network Control ¨ Andreas Freier, Ralf Hofestadt, Matthias Lange, and Uwe Scholz 49 II Gene Regulation: From Sequence...

Ngày tải lên: 08/04/2014, 12:45

... questions of gene regulation and metabolism without explicit mention of the connection between networks and the phenotype One of the challenges, computationally, in understanding gene regulation ... approaches to metabolism and gene regulation This is an area of computational biology that welcomes new methods, ideas, and approaches with the goal of generating a better understanding of the complex ... Evgenia V Kriventseva, and Nicola Mulder vii 17 19 Information Fusion and Metabolic Network Control ¨ Andreas Freier, Ralf Hofestadt, Matthias Lange, and Uwe Scholz 49 II Gene Regulation: From Sequence...

Ngày tải lên: 11/04/2014, 00:45

Ngày tải lên: 06/08/2014, 19:21

... decondensation, and the regulation of apoptosis [13,26-35] Furthermore, genetic analysis revealed that knockouts within the mouse SUN1 gene disrupted the expression of piRNAs and caused a misregulation ... SUN-domain genes as shown in Table and Figure Three of these genes (ZmSUN1, 2, and 3) are distributed as unlinked loci that map to two different chromosomes; ZmSUN4 and ZmSUN5 reside in adjacent genetic ... analysis of intron and exon structures within the maize SUN genes showed that the gene structures are conserved within each class The CCSD genes had two or three exons, and the SUN domain was split...

Ngày tải lên: 11/08/2014, 11:21

... co-expressed gene sets as well Examples are GATA factors, NFATs and HAND1 in heart development; HNF-1 and HNF-4 in liver-specific gene expression; PU.1 in lymphocyte specific gene expression; and myogenin, ... to the quality of the input genes The ratio of true positive genes (containing the smooth muscle cisregulatory module [CRM]) to negative genes (approximated by random genes) was varied Each time, ... constant at ten, and the number of negative genes was varied Red: the total number of genes was kept constant at ten, and the ratio negative genes/positive genes was varied Sensitivity to noise To...

Ngày tải lên: 14/08/2014, 08:21

... N AND AND Model C N C AND Gene Y Gene Z reviews AND comment Model Gene W (CN independent) Gene X (CN dependent) (CN dependent/independent) (CN dependent/independent) (b) W X Y Z C 1 reports Gene ... dependent and independent component to carbon and nitrogen regulation Two examples of Model are shown (genes Y and Z) For gene Y, nitrogen only has an effect in the presence of carbon, while for gene ... genes and processes that are regulated by carbon and nitrogen signaling in plants and the mechanism by which this occurs is still lacking Previously, microarrays were used to identify genes and...

Ngày tải lên: 14/08/2014, 14:21

... (Figures and 3) Because cooperative and competitive interactions are common in gene regulation, the explicit consideration of concentration is likely to be necessary for a complete understanding of gene ... experimicroarraySum1aofORFs)genesaregulatedprobabilitydata from chrogenes) published and( intersectionvitro [7] derived matrix both publishedbyNdt80pand Fkh1p binding [5] sets, andgeneschromatin ments ... distinguish regulated genes from other genes that have a comparable number of binding sites [24-26] GOMER, on the other hand, can distinguish genes with clustered binding sites from genes whose sites...

Ngày tải lên: 14/08/2014, 14:22

... epigenetic gene regulatory mechanisms Nutrition is important in providing methyl donors for DNA, and some genes are especially sensitive to nutritional changes during embryogenesis Rob Waterland ... sequences and epigenetics, are involved in controlling meiotic recombination in humans Nutritional influences during prenatal and early postnatal development may affect gene expression, and subsequently ... enhancers in c-Myc and N-Myc, genes that play a role in growth control and tumorigenesis From the same group, Mikael Björklund presented a genome-wide RNA interference (RNAi) analysis of genes that are...

Ngày tải lên: 14/08/2014, 17:22

... of gene regulation by cohesin is vertebrate specific Epigenetic regulation of inflammatory gene expression Two talks addressed the role of epigenetic mechanisms in regulation of inflammatory gene ... signal transduction and gene regulation control diverse and fundamental biological processes This was underscored at the 2008 meeting We look forward to the next meeting in 2010, and anticipate follow-up ... the Rag genes Later on, an Ikaros variant evolved that could refine activation of the Rag genes, thereby allowing for the development of modern B and T cells Global analysis of chromatin structure...

Ngày tải lên: 14/08/2014, 20:22

... of genes encoding cytokines (IL-1 and TNFα) and growth factors (VEGF and CSF) These secreted cytokines and growth factors bind to the receptors expressed on adjacent tumour cell surface, and ... and 36, and IκBβ on serine 19 and serine 23, leading to its ubiquitination by βTrCP proteins and degradation by the 26S proteosome, and the release of NF-κB from the NFκB-IκB complex (Karin and ... H3 and H4), therefore influencing chromatin structures and transcriptional activity on DNA (Wu and Grunstein, 2000; Jenuwein and Allis, 2001) The opposing actions of histone acetylases (HATs) and...

Ngày tải lên: 14/09/2015, 08:45

... gene expression and transcriptional regulation The amylase promoter will be cloned and characterized, and a series of deletion mutants will be generated and fused to the luciferase reporter gene, ... of amylase gene expression in seabass larvae and mechanism of gene regulation There were three specific objectives: To clone and sequence the α-amylase gene of seabass (both cDNA and genomic ... are generated by differential splicing, while two genes exist for the two flo under TRα transcripts In contrast, mammals and chicken TRα arise from one gene and TRβ from the other gene, and additional...

Ngày tải lên: 17/09/2015, 17:20