gene expression of carotenoid oxygenases in adult vertebrates

... threshold, to promote and maintain rolling interactions [28] In our study, expression of L-selectin was depressed in the HT group This finding is consistent with previous findings and suggests that ... cytokine interleukin-10 (IL-10), chemokine interleukin-8 (IL-8), intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractive protein-1 (MCP-1), tissue factor (TF), integrin cluster of ... of hypertonic fluid administration on the gene expression of inflammatory mediators in circulating leucocytes in patients with septic shock: a preliminary study Annals of Intensive Care 2011 1:44...

Ngày tải lên: 20/06/2014, 23:20

... maintenance of surfactant protein expression in adult human ATII cells in monolayer culture has been difficult Recently, we developed a system for maintaining the differentiated functions of adult ... done with adult human ATII cells cultured with d of TGFa and d of KGF Similar findings were observed in ATII cells cultured in KGF (K) alone, in d of K and d of KD (data not shown) or in d of KIA ... production of mature SP-B Napsin A and pepsinogen C, asparatic proteases, are also involved in the processing of proSP-B [53,63] We found no changes in the protein levels of napsin A or pepsinogen...

Ngày tải lên: 12/08/2014, 11:23

... containing DNA spots (5 lg each) corresponding to internal fragments of ADC or cruzipain genes The latter (used as a control) is a housekeeping gene that encodes the main cysteine proteinase of ... a-amanitin of the transcription of arginine decarboxylase (ADC) and cruzipain genes in transformed Trypanosoma cruzi Permeable parasites were preincubated at °C for in the absence or presence of different ... chromosomal integration [19] After 48 h of transfection, geneticin (G418) was added to the cultures of transformed parasites to select plasmidcontaining protozoa The time course of ADC gene expression...

Ngày tải lên: 19/02/2014, 07:20

... induces the expression of several genes, such as those for GK, phosphofructokinase, phosphoglycerate mutase Activation of expression of fbaA in Synechocystis and PK, including sll1330 [34] In this ... respect to the accumulation of inhibitory metabolites [27,28] and gene expression [29] The expression of 10% of the genes was different in the linear growth phase compared to in the logarithmic growth ... assembly of photosynthetic machinery proteins [32] The SodB protein (Spot 29) catalyzes the dismutation of O2) to H2O2 and O2, in which sodB gene and SodB protein expression are induced by continuous...

Ngày tải lên: 16/03/2014, 04:20

... mechanisms involved in the increase or maintenance of bone mass in OA This study of the IT region of the proximal femur in primary hip OA and non-OA postmortem controls investigated changes in gene expression ... level of COL1A2 mRNA, the corresponding level of COL1A1 mRNA was greater in OA samples Per unit of COL1A2 gene expression, the level of COL1A1 gene expression in OA was almost double that in the ... joint cartilage degeneration supports the concept of generalised involvement of bone in the pathogenesis of OA 10 11 12 13 14 Competing interests The authors declare that they have no competing...

Ngày tải lên: 09/08/2014, 08:23

... rendering them active [16] Several lines of evidence point to the involvement of the mitochondrion and the Bcl-family of proteins in the induction of lymphocyte apoptosis during sepsis Overexpression ... leucocytes in sepsis patients and caspase-8 is active [22] in severe sepsis in animals Inhibition of Fas or silencing of caspase-8 inhibits apoptosis during sepsis [9] Interestingly, cross-talk of Fas-receptor ... Figure Expression of Bcl-2 severe sepsis Expression of Bcl-2 inin severe sepsis The expression of the Bcl-2 protein was measured by flow cytometry in CD4+, CD8+ and CD19+ lymphocytes In CD4+...

Ngày tải lên: 13/08/2014, 11:23

... increase in gene expression These data suggest that the effect of AG28262 is targeted towards ALT gene regulation resulting in increased synthesis of ALT enzyme in the hepatocytes The source of serum ... demonstrated that gene expression profiling serves as an important indicator of potential toxic effects in the absence of apparent toxicity [16] Page of Collection of samples for gene expression analysis ... AG28262, a VEGFR-2 inhibitor, in treated rats in the absence of morphological changes in the liver The results of this investigation suggests that the source of increased serum ALT in AG28262 treated...

Ngày tải lên: 13/08/2014, 13:20

... central portion of TR and has two zinc fingers, each composed of four cysteines coordinated with a zinc ion The integrity of each zinc finger is critical, as deletion of zinc fingers or amino acid substitution ... DNA-binding domain containing two “zinc fingers” (DBD), a hinge region containing the nuclear localization signal, and a carboxy-terminal ligand-binding domain (LBD) (Yen, 2001) Cloning of TRα and ... Cloning of TRα and TRβ 2.4 DISCUSSION Tilapia TRα1 and TRβ2 showed a high level of sequence homology in the DNA binding domain and ligand binding domain with the corresponding TR genes of other vertebrates...

Ngày tải lên: 16/09/2015, 12:08

... cathepsin L genes family…… 26 Figure 2.6 Sequence motifs of the two main regions defining silicatein genes…………………….28 Figure 2.7 Phylogenetic relationship between silicatein and silicatein like genes ... 1998) In T aurantia, two silicatein protein isoforms have been identified, silicatein α, and silicatein β (Shimizu et al 1998) In the following years, gene encoding silicatein α and silicatein β ... position of domains in proteins and genes 2.3.2 Gene architecture analyses Silicatein and cathepsin L genes in A queenslandica, S domuncula (silicatein α and β) and L baicalensis (Silicatein α1,...

Ngày tải lên: 13/10/2015, 14:07

... important regulators of cell apoptosis, it is possible that HBV infection might alter the cIAPs gene expressing of liver cell, finally instigating the carcinogenesis of the cells it infected This is ... expression in the HBV expressing cells was first investigated by the comparison of the gene expression profile in the HepG2.215 and HepG2 cells using gene array technology HepG2.215 is a cell line that ... cIAP1 bands were indicated β-Actin was used as a loading control as well A B Int J Med Sci 2005 2(1) 33 The increase of gene expression of cIAP2 in HepG2.215 cells was further investigated by...

Ngày tải lên: 02/11/2012, 11:17

... bacteria in these conditions The time courses of NH4+-N and NO2+3 N in batch experiment are shown in Fig Gradual decrease in NH4+-N and increase in NO2+3 N were observed just after the beginning of ... set specific for amoA gene of AOB belonging to the ß subclass of Proteobacteria The thermal profiles included an initial denaturing step at 95°C for 10 followed by 45 cycles of denaturation at 95°C ... of amoA mRNA in Run should be related to the fact that 40% of ammonia remained at the end of batch experiment Small decrease in the amount of amoA mRNA in Run was observed due to low dosage of...

Ngày tải lên: 05/09/2013, 10:15

... and eosin staining We examined the expression of these proteins in a cancer-free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, all of which were observed in the ... uterine cervix Although it remains to be determined how enhanced expression of Mcm proteins affects DNA replication in cancer cells, the following findings suggest a possible involvement of Mcm in ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa...

Ngày tải lên: 20/02/2014, 23:20

... an important role in controlling gene expression Using transcriptional profiling, the objective of this work was to gain a better understanding of the amino acid control of gene expression As our ... involved in the GCN2-independent regulation of gene expression Our results show that rapamycin, an inhibitor of mTORC1, regulates the expression of a set of genes almost as large as the set of ... unique, signaling pathway involved in the up- and down-regulation of gene expression in response to amino acid starvation Results Amino acid starvation triggers changes in gene expression In order...

Ngày tải lên: 07/03/2014, 03:20

... the control of the GAL10 promoter The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene under ... level of I7 receptor expression is obtained in induced strains at 15 °C while the levels of Golf and Ga15 not change significantly In addition, the protein levels of I7 OR, Golf and Ga15 in strains ... function of increasing amount of total proteins in membrane fractions from I7 yeast strain induced at 15 °C, and from yeast expressing SSTR2 as a control activity By taking advantage of structural...

Ngày tải lên: 07/03/2014, 16:20

... role of the N-terminal part of the kinase, containing a PAS domain, was established in signal transduction between the RH and the kinase [6,7] Addition of H2 to HupUV before or during the incubation ... E-value of 1.2e-140) The presence of the hupR gene in T roseopersicina is in apparent contradiction with the absence of a hydrogen-dependent regulation of HupSL expression In order to examine in detail ... synthesized in the dark due to a single point mutation in the helix–turn–helix DNA binding motif of PpsR, rendering it inactive [26] Comparison of HupSL regulations and the functional roles of HupTUV in...

Ngày tải lên: 16/03/2014, 23:20

... protein metabolism in these cells Histone is rich in the basic amino acids, lysine and arginine, but contains no cysteine Protamine, which is replaced for hitstone via transit proteins during ... Thus, a high expression of GR in Sertoli cells can be fully confirmed Changes of GR expression in testes during sexual maturation To examine relationships between the expression of GR with sexual ... protein and the mRNA were faint Effects of inhibitors of cGCS and GR on primary cultured testicular cells To investigate the contribution of de novo synthesis and the recycling of GSH to the intracellular...

Ngày tải lên: 17/03/2014, 17:20

... diagram of the fusion of cells producing individual protein units of a multisubunit protein Three transfected CHO-K1 cell lines producing either IgA, J-chain or SC, respectively, were fused in two ... ratios of multiple protein units, excessive screening is necessary, as each gene introduced into the cell multiplies the complexity of the screening Others have addressed the problem of stochiometric ... thereby eliminate the positional effect [30] of chromosomal integrating vectors However, for stable expression of multiple genes over time, the usefulness of such episomal vector is limited, mainly...

Ngày tải lên: 18/03/2014, 01:20

... protein kinase Mitogen-activated protein kinase Mitogen-activated protein kinase 11 Defensin, beta Defensin, beta 103A Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta ... (GTP binding protein, 25kda) Wiskott–Aldrich syndrome-like Wiskott–Aldrich syndrome protein interacting protein v-raf murine sarcoma viral oncogene homolog B1 Mitogen-activated protein kinase kinase ... Mitogen-activated protein kinase Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) Rho-associated, coiled-coil containing protein kinase Ras-related C3 botulinum toxin substrate...

Ngày tải lên: 22/03/2014, 16:20

... as neomycin or hygromycin, positive transfectants can be selected in media containing the corresponding antibiotic Induction of gene amplification using glutamine synthetase22 or dihydrofolate ... with the intended use (in vitro, in vivo, structural biology) The design of a suitable expression vector for an expression system of choice, also including a potential "tagging" of the protein to ... for screening, also need to be developed An experiment using an array in which probes to all of the genes in the yeast genome are present generates a minimum of 6000 pieces of data Finally, it...

Ngày tải lên: 11/04/2014, 09:41

... examine the expression of the Tax protein and showed the expression of Tax in synoviocytes in all samples examined Although Tax expression was not correlated with cytokine concentration in SF, ... or definite osteophytes with moderate joint space narrowing; IV, severe with large osteophytes and definite joint space narrowing bScoring was as follows: +/-, minimum staining in one area of the ... tissue; +, patchy staining involving several areas; ++, moderate diffuse staining OST, osteotomy; SYV, synovectomy; TA, total joint arthroplasty Of the cytokines examined in our study, C-PTHrP...

Ngày tải lên: 09/08/2014, 01:23