gcn5 pcaf hat domains support an ordered sequential reaction mechanism and the role of ygcn5 glu
techniques for immune function analysis
Ngày tải lên :
11/04/2014, 10:34
... better define and understand the networks of cellular and molecular mechanisms that underlie immunity and inflammation in health and disease BD Biosciences is pleased to introduce the new Techniques ... Panels A and B), PerCP-anti-human CD8 (custom made by the Custom Technology Team, BD Biosciences Pharmingen, Panels C and D), APC-anti-human CD45RO (Cat No 559865, all Panels) and PE-anti-human ... either purified anti-human TLR1 (clone GD2, Cat No 552033, Panel A), or purified anti-human TLR4 (clone HTA125, Cat No 551964, Panel B) The anti-human TLR1 and anti-human TLR4 antibodies were then...
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Senescence and Cell Cycle Control
Ngày tải lên :
25/10/2013, 21:20
... combination of SV40 large T-antigen (T-Ag), activated Ras and the telomerase catalytic subunit (hTERT) can transform HDFs and human keratinocytes (Hahn et al 1999) T-Ag inactivates the p53- and Rb-dependent ... and Xianghong Zou for helpful discussions, and the National Institutes of Health, the Department of Defense and the American Cancer Society for grant support for my research References Bates S, ... replicative life span of human fibroblasts by p16 and the polycomb protein Bmi-1 Mol Cell Biol 23:389–401 Itahana K, Campisi J, Dimri GP (2004) Mechanisms of cellular senescence in human and mouse cells...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... from the global t The constant a is the ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one ... phosphorylation and degradation, and the subsequent translocation of NF-jB into the nucleus may be mediated by 1684 A E C Ihekwaba et al another mechanism Such a mechanism could be derived from the theory ... bottomed plates and treated with ngặmL)1 IL-1a in the presence and absence of the SC-514 inhibitor (D, E) Time course plot of nuclear NF-jB from in vitro and in silico analysis of the data The plot...
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Báo cáo sinh học: "Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression" ppt
Ngày tải lên :
06/08/2014, 18:20
... assess the rate of protein degradation Each point represents the mean and range of three independent cultures The rate of protein degradation is indicated by the slope of the line The shallowness of ... allows, and it must quickly adapt to changing extracellular conditions The growth and division of animal cells, by contrast, must be carefully controlled and coordinated for the good of the animal ... for instance, the size of the disc cells varies throughout development: the cells initially grow without dividing and then proliferate and get progressively smaller [22] Why yeasts and Schwann cells...
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Báo cáo y học: "Two specific drugs, BMS-345541 and purvalanol A induce apoptosis of HTLV-1 infected cells through inhibition of the NF-kappaB and cell cycle pathways" ppsx
Ngày tải lên :
10/08/2014, 05:21
... compare these different classes of inhibitors against one another In Table 1, they are ranked as high, moderate, and poor inhibitors and the reported activities of these molecules against variety of ... E Agbottah and W-I Yeh contributed equally to this work and share first authorship We thank Ann Richmond and Dean Ballard (Department of Cancer Biology, Vanderbilt University) for the expression ... with the latest progress in the understanding of HTLV-1 infection, its pathogenesis and its mechanism of action, more progress in developing therapies for these infected cells is needed There...
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Báo cáo y học: "Spore germination in Saccharomyces cerevisiae: global gene expression patterns and cell cycle landmarks" potx
Ngày tải lên :
14/08/2014, 08:20
... DZ, GS and NB conceived and designed the experiments DJS performed the experiments DJS, GS and NB analyzed the data and wrote the manuscript DZ provided detailed advice and commented on the text ... identify the border between the growing and non-growing domains ('halves') of the spore By merging RD and GFP signals it can be seen that Cdc10 is localized to the border that separates the growing and ... precursors The changes in the expression of gluconeogenesis and stress genes reflect the shift to glucose metabolism and the release from stress Similarly, genes that are related to the TCA cycle and...
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The role of TRIP br proteins in the regulation of mammalian gene transcription and cell cycle progression
Ngày tải lên :
16/09/2015, 17:12
... significance 1.2.2 The role of PHD zinc fingers and the bromodomains 10 in the regulation of eukaryotic gene transcription Transcriptional regulators interacting with the PHD zinc finger 15 and/ or the ... reveals that the PHD finger of CBP forms 11 an integral part of the enzymatic core of the HAT domain The requirement of an intact PHD finger for CBP to execute its HAT and FAT (Factor Acetyltransferase) ... as transcriptional activity, further highlights the role of PHD zinc fingers in chromatin-dependent transcription 14 On the other hand, the bromodomain of the histone acetyltransferase (HAT) ...
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Involvement of nm23 m2 in dopaminergic neuronal differentiation and cell cycle arrest
Ngày tải lên :
08/11/2015, 17:09
... sincere help and technical support in one way or another Last but not least, I would like to thank my loved ones, my parents and my sister for their love and understanding Special heartfelt thanks to ... 4oC for 30 The supernatant was then discarded by pipetting The pellet was washed with 200µl of 70% ethanol and centrifuged for at 4oC, 14,000 x g The supernatant was then discarded and the pellet ... as in the West out of every thousand individuals, aged 50 years and above, will have this disease Prevalence of PD was also investigated between Singapore Chinese, Malays and Indians and the environmental...
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Báo cáo y học: "Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle" docx
Ngày tải lên :
13/08/2014, 23:20
... which goes down to 2N and back to 4N and never repeats the 8N In between the start and the end there is no systematic variation of cells through G1, S and G2 In summary, the work of Whitfield et al ... object model) standards, so that the public data can be analyzed 18) Microarray results should be compared to randomized data to show that the observed cyclicities are not the result of random noise ... good experiment The problem is whether the methods of Whitfield et al lead to an understanding of what happens in the normal, unperturbed cell Theory says they are not synchronized and experimental...
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Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor
Ngày tải lên :
25/10/2013, 21:20
... models of cancer are both impressive and powerful, they not necessarily reflect the spectrum or complexity of human cancer Only a small amount of human cancer can be attributed to the inheritance of ... for understanding the commonality of these mechanisms even in vertebrates Nurse and colleagues then cloned human Cdc2 through complementation of the yeast cdc2 mutant By studying changes in protein ... in the fertilization of clam and sea urchin eggs, Hunt and colleagues identified the first cyclin proteins, the abundance of which fluctuates with the division of the eggs The identification of classes...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
Ngày tải lên :
25/10/2013, 21:20
... in development of cancer (reviewed in Weinberg 1995; Hanahan and Weinberg 2000; Harbour and Dean 2000) The molecular cloning of two other Rb-like genes, p107 and p130, defined the retinoblastoma ... functions and interactions The difference in phosphorylation sites and kinases involved in the phosophorylation of these sites between p107 and p130 further support specific functions for p107 and p130 ... shaded areas) Within the pocket domain (pocket subdomains A and B and the spacer region) the highest homology between the pRb protein family is found in the A and B subdomains The pocket-domain...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
Ngày tải lên :
18/02/2014, 16:20
... mass at the bud neck and the number of anaphase cells were increased slightly in the mutant (metaphase cells: 30.2% in the mutant versus 24% in the wild-type; anaphase cells: 4.5% in the mutant versus ... wild-type and mutant (D244N) proteins are likely attributable to simultaneous autophosphorylation of Rio1p and ⁄ or the action of still another protein kinase (M Angermayr, unpublished observations) The ... is simultaneously required in the G1 phase and for the onset of anaphase (and ⁄ or nuclear division and chromosome segregation) [12] Vanrobays et al [17] obtained evidence from a synthetic lethal...
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Financial Intermediation and Credit Policy in Business Cycle Analysis∗ pot
Ngày tải lên :
06/03/2014, 08:20
... the spirit of Allen and Gale (1995, 2007) and others This is because within our framework (i) only banks of the same island can make loans to nonfinancial firms and (ii) banks on investing islands ... Before they move, they sell their existing loans to nonfinancial firms to the other banks that remain on the island in exchange for inter-bank loans that the remaining banks have been holding in their ... of net assets the bank can divert and negatively on the excess value of bank assets, given by μt The higher the excess value is, the greater is the franchise value of the bank and the less likely...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
Ngày tải lên :
07/03/2014, 16:20
... amplification of hCdt1 and h-geminin cDNAs and 20 cycles for the amplification of h-actin cDNA The number of cycles was adjusted to ensure that the reaction was in the linear range PCR products were analyzed ... amounts of recombinant full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti-Cdt1 and anti-geminin ... tumorigenic and matched normal specimens thereby licensing DNA for a further round of DNA (kidney cancer, liver cancer and lung cancer and respective replication When G1 cells exit the cell cycle and...
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Báo cáo khoa học: Injection of poly(b-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate pot
Ngày tải lên :
16/03/2014, 18:20
... propose that the underlying mechanism by which PMLA increases the growth rate and shortens the duration of the cell cycle is the polymer-inherent isosterism of the carboxylates with the array of phosphates ... until the end of the experiment We then tested whether the plasmodial size (growth rate, Fig 2A,C,E) and the duration of the cell cycle (Fig 2B,D,F) depended on the dose of injected PMLA, over the ... between the two white strains on one hand and the two yellow strains on the other the different relative increases of PMLA in the cytoplasmic extracts from yellow and white plasmodia can be seen...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
Ngày tải lên :
17/03/2014, 23:20
... replace that fraction of hCdc6p that is transferred to the cytoplasm and eventually degraded It can therefore be predicted that the amounts of hCdc6p on chromatin increase during G1 Fig Soluble and ... the upper band corresponded to hCdc6p whereas the lower of the two bands was unspecific (because the secondary mouse anti-rabbit Ig react with an unknown cellular protein (Fig 1A, lane 3) We analysed ... well as at the end of mitosis and during the early G1 phase (12–14 h) of the next cycle (Fig 2A) Cytoplasmic and chromatin extracts were immunoprecipitated with specific antibodies and transferred...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
Ngày tải lên :
23/03/2014, 06:20
... during the cell cycle In order to understand the molecular mechanism of the differential regulation of Hox gene expression, we analyzed the changes in H3K4 methylation and recruitment of MLL1 and ... progression, we analyzed the expression profiles of MLL1, CGBP, Ash2 and Rbbp5, together with cyclins E and B, as a function of the cell cycle Western blot analysis of the whole-cell extract and histones ... 6C,D) In the case of the HoxA10 gene, significantly higher levels of RNAP II and MLL1 recruitment and H3K4 trimethylation marks were observed at the beginning of the S phase (0 h), and these marks...
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Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc
Ngày tải lên :
30/03/2014, 20:20
... increase of the 2.5-kb cyclin B1 transcript cannot be explained by half-life changes It is possible that the majority of the regulation of these transcripts may reside in the choice of polyadenylation ... FEBS The purpose of the present study was to analyse the contribution of post-transcriptional mechanisms in the cell cycle regulation of cyclin mRNAs Previous studies on HeLa cells [11,12] and ... revealed that there is more than one transcript from mouse cyclin A2 and B1 genes In the case of mouse cyclin A2, besides the more abundant mRNA of 1.8 kb, there is a minor species of 3.0 kb...
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Báo cáo khoa học: The inhibition of Ras farnesylation leads to an increase in p27Kip1 and G1 cell cycle arrest pdf
Ngày tải lên :
31/03/2014, 01:20
... the rate of synthesis of the protein [25,29,39]; (b) variations in the rate of degradation [40] and (c) transcriptional control [41] To evaluate the contribution of HR12 to the stability of the ... and with anti-actin Ig as a control The diagram shows quantification of the intensity of the p27Kip1 bands, calibrated to the intensity of the actin bands, where the zero time value was designated ... report upon the effects of HR12 on growth and on the cell cycle We find that HR12 suppresses anchorage-dependent and independent growth and motility of Rat1/ras (Figs and 2) Furthermore, treatment...
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cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
Ngày tải lên :
08/04/2014, 12:50
... The Budding and Fission Yeasts Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts Tim Humphrey and Amanda Pearce Summary The cell cycles of the budding yeast ... pronounced band of microtubules, called the preprophase band (PPB), develops in the area of the presumptive division plane The site of the PPB accurately predicts the division plane, and the correlation ... to animals and fungi, there are a number of peculiarities that are perhaps related to their lifestyle and development Initial comparisons of animal, fungal, and plant genomes suggest that the...
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