fundamentals and principles of biomolecules in adipose stem cell engineering

... Malaysia) and Margam Chandrasekaran (Singapore Institute of Manufacturing Technology, Singapore) Vol.4 Series on Biomaterials and Bioengineering Fundamentals and Applications of Biophotonics in Dentistry ... Properties of Dental Enamel and Dentin 9.4.1 Structure of Enamel and Dentin 9.4.2 Spectral Properties of Enamel and Dentin 9.4.3 Scattering Properties of Enamel 9.4.4 Scattering Properties of Dentin ... some of the features and adaptation of photonics in this area Dental photomechanics provides an understanding of mechanical and thermal characteristics of dentine and permits a better understanding...

Ngày tải lên: 22/03/2014, 21:21

... L-glutamine, 10mg/mL streptomycin and 10U/mL penicillin The cells were cultured in an incubator at 37°C, 5% CO2 in a humidified environment 2.1.2.1.2 Over-expression of SPHK1 and SPHK2 in CHO Cells ... Human SPHK Inhibitors Over-expression of SPHK1 and SPHK2 was achieved by transfecting the SPHK1 or SPHK2 plasmids into CHO cells using Lipofectamine TM 2000 (Invitrogen), following the instructions ... could be tested and validated in animal models of diseases However, due to the limited duration of PhD study, and the requirement of Graduate Program in BioEngineering, investigations of the synthetic...

Ngày tải lên: 11/09/2015, 16:06

... Roles of SPHK Inhibitors in Human BM- and AD- MSCs Differentiation inhibitors Within these results, 1μM of DMS triggered a higher staining than 0.5μM of DMS; and 5μM of CP6 triggered higher staining ... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization ... 3.2.3.1.2.2 Immunostaining of Osteocalcin, Osteopontin, and osteonectin The expression of osteocalcin, osteopontin, and osteonectin was measured by immunostaining after 28 days of culture with or without...

Ngày tải lên: 11/09/2015, 16:06

... 1μM of S1P and 20ng/ml PDGF-AB promoted around 2-fold increase in cells growth, compared to the cells grown in DMEM only 4.2.3 Stemness Maintenance of Cells Cultured with S1P After identifying ... would be convincing that the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally ... signaling pathways; and part of it will penetrate into the cells and directly act as an intracellular second messenger Both functions of S1P contributed to the intracellular calcium release, and...

Ngày tải lên: 11/09/2015, 16:06

... the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo work, ... conclusions Finally, the study could be expanded to study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, ... for SPHK inhibitors in adult -stem cell differentiation In the final part of my research project (Chapter 4), the bioactive lipid S1P was studied for its role in promoting human BM- and AD- MSCs...

Ngày tải lên: 11/09/2015, 16:06

... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25 ... "Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival." J Cell Biol 147(3): 545-58 Olivera, A and S Spiegel (1993) "Sphingosine-1-phosphate ... roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the...

Ngày tải lên: 11/09/2015, 16:06

... roles of SPHK and S1P in stem cell proliferation, stemness maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness ... human stem cells and the strategies used for stem cell expansion and differentiation, are discussed below 1.4 HUMAN STEM CELLS 1.4.1 Brief Introduction of Human Stem Cells Diverse stem cells and ... markers, maintained by S1P+PDGF Thus, it indicates the involvement of endogenous SPHK and/ or S1P in stem cell proliferation and multipotency maintenance It is well known that in stem cells, there...

Ngày tải lên: 11/09/2015, 16:06

... Seng, Huibin, Yin Loon, Minh, Sandy, Phil and all other past and present members of Bing’s lab, thank you for your friendship, and for making the lab such a stimulating and fun place to work in I ... receptor Multiple cloning site Methyl-CpG-binding proteins Mouse embryonic stem cell Midbrain-hindbrain isthmus/boundary MicroRNA MicroRNA MicroRNA-containing RNA-induced silencing complexes MicroRNA ... synthase kinase-3 High content screening Histone deacetylases Human embryonic stem cell Inner cell mass Inhibitor of differentiation In situ hybridization Janus-associated tyrosine kinase Leukemia inhibitory...

Ngày tải lên: 12/09/2015, 08:16

... understanding of the regulation of stem cell properties is critical for improving our ability to manipulate normal stem cells in vitro and in vivo Furthermore, understanding normal stem cell regulation ... into two main groups; embryonic stem cells (ESC) and adult stem cells (ASC) (Sylvester and Longaker, 2004) The main distinctions between 23 cells from these groups are in terms of residency and ... retaining’ (Cotsarelis et al., 1990) Similar observations were made in other systems containing stem cells, including the gut epithelium and epidermis (Cheung and Rando, 2013) Experiments in the...

Ngày tải lên: 22/12/2014, 21:11

... upregulation of fatty acid oxidation in brown adipose tissue following the over expression of PPARδ in terms of the expression of a range of enzymes involved in uncoupling (uncoupling protein and 3), ... of PPARg and PPARδ in regulating metabolism in white adipose tissue, we have Page of 19 performed a metabolomics study using both in vivo analysis in the ob/ob mouse and in vitro analysis using ... 6-desaturase is integral to both pathways; the enzyme introduces the initial double bond into linoleate forming g-linolenate in the ω-6 pathway and introduces the double bond into linolenate forming stearidonic...

Ngày tải lên: 09/08/2014, 23:20

... inflammation in these depots Because visceral adiposity is linked to insulin resistance, induction of SLPI locally in adipose may also play a role in the prevention of inflammation-induced insulin resistance ... stabilization of IKBa abundance Activation of TLR2 and increased expression of SLPI in macrophages [10,24] and in adipocytes as confirmed in this study Therefore, because TLR2 and TLR4 are activated in adipose ... role in obesity-induced insulin resistance by the release of multiple inflammatory cytokines that oppose insulin signaling [14] Although the endogenous mechanisms that trigger adipose tissue inflammation...

Ngày tải lên: 11/08/2014, 03:20

... inflammation in these depots Because visceral adiposity is linked to insulin resistance, induction of SLPI locally in adipose may also play a role in the prevention of inflammation-induced insulin resistance ... stabilization of IKBa abundance Activation of TLR2 and increased expression of SLPI in macrophages [10,24] and in adipocytes as confirmed in this study Therefore, because TLR2 and TLR4 are activated in adipose ... role in obesity-induced insulin resistance by the release of multiple inflammatory cytokines that oppose insulin signaling [14] Although the endogenous mechanisms that trigger adipose tissue inflammation...

Ngày tải lên: 11/08/2014, 06:22

... with exposure to loratadine and other antihistamines according to the time of exposure The adjusted OR for loratadine exposure within 30 days before conception and during the first trimester ... complete and independent registration of birth, birth outcome, and prescription data which prevented selection bias and some types of information bias; since the study was based on routinely recorded ... number, and the date of drug dispension Since January 1989 all data from North Jutland County have been stored in a prescription database maintained by the Department of Clinical Epidemiology,...

Ngày tải lên: 02/11/2012, 10:09

... Journal of World Trade 34 (1): 141–166 Estevadeordal, Antoni and Kati Suominen 2006 “Mapping and Measuring Rules of Origin Around the World.” In The Origin of Goods: Rules of Origin in Regional ... quarter of the welfare gain at a cost of 13.3 percent of the output loss, while elimination of preferential rules of origin accounts for the remaining 23.3 percent of increased welfare and 84.9 ... USAGE-ITC distinguishes 523 commodities, 521 industries, 23 foreign regions, and a detailed handling of margins and taxes 28 Other features of the model include a detailed modeling of government...

Ngày tải lên: 12/04/2013, 16:17

... System System operates over the domains of units: there are systems of sentences, of clauses, of groups, of words, and of morphemes Basing on the source language, students are taught to distinguish ... chapter examines an opinion of negative interface by giving the overview of negation in English and Vietnamese and the preview of syntax and semantics theory The overview of classification of negation ... overview of syntactic and semantic features of negation in both English and Vietnamese 12 - Studying negation in terms of its structures and semantics and finding out the similarities and differences...

Ngày tải lên: 11/12/2013, 23:53

... Sub In the class module for the form, add the code in Listing 6.7 to create the GenerateData routine After creating the SQL statement, this routine assigns it to the Text property of lblSQLString ... SQL String Label Name lblSQLString Label Text Results DataGrid Name dgResults TextBox Add the following code in Listing 6.6 to the Load event of the form (Double-click on the form to bring up ... code in Listing 6.8 to the TextChanged events of txtCustomerID, txtFromDate, and txtToDate, respectively These routines call GenerateDate when the values change Listing 6.8 frmHowTo6_3.vb: Calling...

Ngày tải lên: 14/12/2013, 20:16

... powerful influence in shaping our thinking and behaviour As its simplest, culture can be defined by Guirdham as shared ways of seeing, thinking and doing or “a historically transmitted system of symbols, ... as the US, pink is the colour of little girls It represents sugar and spice and everything nice Pink for men goes in and out of style Most xxxvii people still think of pink as a feminine, delicate ... wedding celebrations Red in that culture is the symbol of happiness and ultimate joy, thus all Chinese weddings include the colour red English find many meaningful definition for colours used in...

Ngày tải lên: 29/01/2014, 14:39

... species of benthos and about 33 species of fish According to Vietnam standard of water quality, the important parameters of water quality of West Lake, including: Phosphorous and Nitrogen concentration, ... of West Lake is controllable if a sufficient amount of fish (< 5g/l) is maintained in the lake To maintain a sufficient amount of fish, it is necessary to plan time to catch fish and amount of ... each, there is an exporting coefficient, representing the average amount of Phosphorous in every hectare of land for the lake per year By using these coefficients and data of West Lake, we found...

Ngày tải lên: 13/02/2014, 12:20

... development of cage culture methods/designs for groupers in Malaysia, although large-scale cage farming in marine waters really gained ground in the 1980s and in inland waters in the 1990s (Shariff and ... Philippines India Philippines China Philippines Philippines Vietnam Japan Japan China Philippines Sri Lanka Japan Malaysia Philippines Vietnam Malaysia Philippines Singapore Thailand Philippines ... difficulties of disease and parasite treatment Risks of theft are increased Amortization of capital investment may be short Increased labour costs for handling, stocking, feeding and maintenance...

Ngày tải lên: 14/02/2014, 17:20

... routine urinalysis In patients with renal or genital tuberculosis, urinalyses are abnormal in more than 90%, the main finding being pyuria, and/ or hematuria The finding of pyuria in an acid urine ... multiple-puncture), the kind and dose of tuberculin, and the size of reaction in millimeters of induration D Interpretation of Skin Test Reactions To interpret the tuberculin skin test appropriately, ... presenting symptom of skeletal tuberculosis is pain (62) Swelling of the involved joint may be noted, as may limitation of motion and, occasionally, sinus tracts Systemic symptoms of infection...

Ngày tải lên: 15/02/2014, 12:20