functionalization of s layer supported lipid membranes

... was subtracted using the appropriate blanks To monitor the kinetics of ostreolysin interaction with lipids (SUVs composed of SELs or sonicated lysophospholipids), tryptophan emission intensity ... FTIR spectroscopy was used to assess the secondary structure of ostreolysin in solution or adsorbed to the lipid phase by analysis of the amide IÂ band as described [17] Ostreolysin was incubated ... articial membranes formed of erythrocyte total lipid extracts, suggesting the presence of a specic lipid acceptor (s) When the ability of lipids to inhibit ostreolysin-mediated hemolysis was investigated,...

Ngày tải lên: 23/03/2014, 21:20

... mixed bilayers, again a single resonance was observed, as a consequence of the lower spectral resolution of the system Some variability is expected in the self-assembling process and in the bilayer ... systems, such as proteolipid membranes, that are deposited on this surface, involving changes in surface mass density (i.e the amount of mass per unit surface area), in the spatial mass distribution ... the presence of sphingomyelin, cholesterol and zinc ions Results and Discussion In order to characterize the interaction of Ab with lipid membranes, we used PWR spectroscopy to study the association...

Ngày tải lên: 30/03/2014, 11:20

... occurrence of well-developed abscission layers upon flowering suggests that all six wild Oryza accessions will shatter their seeds readily, an observation that is consistent with our previous measurement ... accessions used in our microscopy study displayed earlier degradation of the abscission layer than what is seen in wild rice, a range of degradation severity seems to exist Previous studies of the sh4 ... tissue samples were excised from the flowers at the topmost end of the panicle using a dissecting scope Microscopy Conclusions Our results show that the shattering trait in U .S weedy rice has...

Ngày tải lên: 11/08/2014, 11:21

... monolayers 7.3.1.2 Isotherms of mixed lipid/ V4 monolayers 7.3.1.3 Miscibility analysis of monolayers 7.3.1.4 Stability analysis of monolayers 7.3.1.5 Compressibility analysis of monolayers 7.3.2 ... kind understanding, continuous support and unconditional love all these years i Table of Contents Acknowledgements Table of contents Summary List of Tables List of Figures List of Symbols CHAPTER ... Regardless of the distinct origins, primary sequences, biological activities and secondary structures, most antimicrobial peptides share some common characteristics They are short peptides, consisting...

Ngày tải lên: 14/09/2015, 21:54

... filaments on the monolayer indicated that V4 might aggregate on the membranes 7.3.4 Insertion of V4 into solid supported bilayer Lipid bilayers are the basic constituent of cell membranes and solid supported ... has its own collapse pressure In a two-component system, if the components were immiscible, two collapse pressures would be observed at the corresponding collapse pressure of pure components ... Isotherms of POPG and POPC monolayers 7.3.1.2 Isotherms of mixed lipid/ V4 monolayers Fig 7.2 shows the surface pressure (π) and molecular area (A) isotherms of POPG/V4 and POPC/V4 monolayers at...

Ngày tải lên: 14/09/2015, 23:32

... vesicles, showing that there was no substantial loss of vesicles Thus the vesicle number Nvesicle (N2) of rhodamine 6G entrapped vesicles is assumed to be the same as that of Rho-PE labeled vesicles ... diffusion time of V4TMR: mixed lipids SUVs complex was similar to that of pure lipids SUVs, between 0.6 and ms Fig 5.7 Binding of V4-TMR to SUVs of mixed lipid composition Shown are ACFs of V4-TMR ... least one hour PBS buffer was added to re-dissolve the lipids to give an aqueous suspension of phospholipids at a concentration of 0.5 mM SUVs were prepared by freeze-thawing the lipid suspension...

Ngày tải lên: 14/09/2015, 23:32

... out by use of the established solid phase peptide synthesis (SPPS) Unnecessary coupling steps can be circumvented and the corresponding products can be achieved in satisfactorily yields and in ... with DARinv The synthesis s steps as well as the chemical reactions were documented in 2009 by the Pipkorn group [31] In this paper we focused our studies at first to the synthesis of the amide ... Atherton s and Sheppard s [17] and Wiessler s documentations (Figure 1) [15] The synthesis of the tetrazine dicarbonic acid derivate was performed as described by [15] The synthesis procedure of the...

Ngày tải lên: 25/10/2012, 11:00

... In contrast to emulsions and liposomes, the particle matrix of SLNs is composed of solid lipids The majority of lipids commonly used are triglyceride esters of hydrogenated fatty acids Hydrogenated ... formulations using a statistical software package (Statistical Analysis System, SAS Institute, 401 Inc., Cary, NC, USA) Differences between related parameters were considered statistically significant ... lipid- based nano-assemblies [13] Solid lipid nanparticles (SLNs) have been proposed as an alternative drug carrier system to other novel delivery approaches such as emulsions, liposomes, and polymeric...

Ngày tải lên: 25/10/2012, 11:22

... assumptions in Equation (2) In spite of this, Equation (6) is valid at the early stage and then can be used to estimate the hydraulic permeability of porous media such as sand layer, glass beads, and ... storage in Europe, Geothermics, 2003,32 (4–6),579–588 [2] Chiasson A, Rees J, Spitler J, A preliminary assessment of the effects of groundwater flow on closed-loop ground-source heat pump systems, ... Hazen A,Some physical properties of sands and gravels, with Special Reference to their Use in Filtration 24th Annual Report of Massachusetts State Board of Health, 1892, 34,539-556 [5] Shepherd...

Ngày tải lên: 05/09/2013, 17:03

... described However, Xenopus egg extract MCMs dissociate from the chromatin as S- phase progresses (Kubota et al., 1997; Thommes et al., 1997) In humans, MCMs also seem to dissociate during S- phase, ... mechanisms and ORC phosphorylation gives the cells a reversible way to prevent replication firing In higher eukaryotes, phosphorylation of ORC subunits is also observed In Xenopus systems, phosphorylation ... cells, Orc1 seems to be mono- and di-ubiquitinated, which causes its release from chromatin in S- phase until M-G1 (Li and DePamphilis, 2002) Regulation of S Phase 37 In humans, several studies show...

Ngày tải lên: 25/10/2013, 21:20

... Des exemples de dispositions dessai sont donnộes dans les figures 2a et 2b Des dispositions dessai pour dautres types sont indiquộes dans la CE1 60269-2-1 : Rốgles supplộmentaires pour les fusibles ... essais Essais nos et 2: Pour chacun de ces essais, il est procộdộ consộcutivement l'essai de trois ộlộments de remplacement Si, au cours de l'essai no 1, les conditions prescrites pour l'essai ... fusibles destin s ờtre utilis s par des personnes habilitộes (fusibles pour usages essentiellement industriels) et la CE1 60269-3-1 : Rốgles supplộmentaires pour les fusibles destin s õ ờtre utilisộs...

Ngày tải lên: 25/12/2013, 10:55

... business systems necessary to identify significant new business opportunities for strategy innovation x All companies must balance the needs of today s business with the needs of tomorrow s business ... has a unique or superior business model Dell Computer is a successful company because of its innovative business model (selling customized computers via the Internet and use of a very strong supply ... process for strategy innovation in a corporate setting is not as simple as it sounds This is especially true when the process ventures outside the current corporate strategy or business model Escaping...

Ngày tải lên: 17/02/2014, 04:20

... phosphatidylserine expression was of the same order of magnitude as the upregulation of CD1d expression In other words, the same HepG2 cells expressed CD1d and phosphatidylserine Although phosphatidylserine ... Darmstadt, Germany) Statistical analyses The paired Student s t-test (two-tailed) was used to test the significance of the differences between group means Statistical significance was defined as P ... viable cells that marks a process related with the cell cycle status [45] In this context, it is important to stress that DNA content studies did not show significant differences in apoptosis (subG0...

Ngày tải lên: 19/02/2014, 16:20

... mechanism of fibroblast stimulation This in turn increases cohesion of the dermal collagen fibers themselves This action means increased thickness, suppleness and resilience, as well as hydration Hydration, ... repair, fast recovery time and optimum performance In cancer patients, as cancer progresses, it often destroys muscle tissue in a very aggressive fashion The muscles of the arms and legs seem to ... in various fields Collagen is a type of protein, fibrous in nature that connects and supports other bodily tissues, such as skin, bone, tendons, muscles and cartilage It also supports the internal...

Ngày tải lên: 21/02/2014, 01:20

... A (1986) Isozyme-specific enzyme inhibitors 10 Adenosine 5¢-triphosphate derivatives as substrates or inhibitors of methionine adenosyltransferases of rat normal and hepatoma tissues J Med Chem ... analog, its nucleotide (s) , methionine -S- adenosyltransferase, AdoHcy hydrolase, and other AdoMet related cycles such as polyamine biosynthesis It is possible that all these interactions contribute ... Kamitori, S & Markham, G.D (1996) Structure and function of S- adenosylmethionine synthetase: crystal structures of S- adenosylmethionine synthetase with ADP, BrADP, and PPi at 28 angstroms resolution...

Ngày tải lên: 08/03/2014, 08:20

... Actinoporins, cytolytic toxins synthesized by sea anemones, comprise a unique group of PFTs [8] They comprise a group of 20 kDa, cysteineless proteins, whose activity depends on the presence of sphingomyelin ... produces 50% of the maximal rate of hemolysis) Deletion mutant D5, S1 C, chemically modified ver+ sions of S1 C (S1 C-MTSEA , S1 C-MTSET+, S1 C– MTSES , in which S1 C was chemically modified with MTSEA+, ... Polar Lipids (Alabaster, AL) All other materials were from Sigma (Milan, Italy), unless stated otherwise Cloning, expression and isolation of the mutants The construction of expression vectors of...

Ngày tải lên: 23/03/2014, 10:20

... kinase Purine nucleoside phosphorylase Phosphoribomutase Phosphoribosylpyrophosphate synthetase D-Ribose-5P-isomerase S- Adenosylhomocysteine hydrolase Transaldolase Transketolase Triosephosphate ... which is difficult to assess in vivo, is involved in these pathways Since adenine is a substrate of ADPRT, the elevation of ATP in the absence of adenosine kinase shows that adenine must be released ... pathway of ATP buildup from SAM, though not by a detailed stoichiometric analysis SAM is converted into S- adenosylhomocysteine (the substrate of SAHH) by enzymes from the class of methyltransferases...

Ngày tải lên: 30/03/2014, 20:20

... lines to data points a weighted nonlinear regression analysis program was used (FIG P for Windows; Biosoft) kcat values are based on a molecular mass of 40 kDa for all proteins Standard errors ... newly opened glass ampoules, 20% solutions (Ladd Research Industries) MS Electrospray mass and tandem mass spectra were recorded on an AutoSpec-OATOFFPD high-resolution magnetic sector-orthogonal ... metabolism Similar reasoning has included other ADHs and their metabolism of various endogenous substrates, e.g certain serotonin metabolites, during ethanol intake [23] The observation of GSSG as...

Ngày tải lên: 31/03/2014, 07:20

... decrease of Ksv was observed for PtdCho/PtdSer vesicles containing 10% PtdSer up to a sixfold to sevenfold decrease for pure PtdSer vesicles Incorporation of cholesterol into PtdCho/PtdSer vesicles ... uorescence intensity of the peptides, upon addition of SUVs The uorescence intensity at 350 nm, expressed as the percentage of the uorescence of the lipid- free peptide was plotted vs the added lipid ... titration measurements Peptidephospholipid interactions were studied by monitoring the changes in the Trp uorescence emission spectra of the peptides upon addition of SUVs Intrinsic uorescence of the...

Ngày tải lên: 31/03/2014, 23:20

Ngày tải lên: 15/05/2014, 02:06