functional identification of neural stem cell derived oligodendrocytes

... mediated by NSC -derived neurotrophins in the lesioned micro-environment of the CNS. Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the cortex of E16 Sprague-Dawley ... induction of functional improvement remains controversial. The ability of neural stem cells (NSC) to differentiate into neural cells has been seen in culture [2]. Given the complexity of both the ... primary neural stem cells from cortex of E16 Sprague-Dawley rat embryos. Figure 2 ELISA of nerve growth factor in one-day spent tissue culture media of culture passages five to seven of primary neural...

Ngày tải lên: 18/06/2014, 16:20

... application of the method produces a set of 4,449 cell and tissue markers, including 45 out of 71 known stem cell markers (69%). Analysis of the markers that segre- gate six types of stem cells (hematopoietic, ... mechanisms of immune system avoidance that are activated in cells undergoing differentiation. This complete set of cell markers offers a good basis from which to study principles of stem cell gene ... described [7]. Table 1 Set of mouse samples selected for our analysis from StemBase Class Samples Replicates SampleIDs Adipose derived stem cells 1 3 S199 Dermis derived stem cells 1 3 S200 Embryonal...

Ngày tải lên: 14/08/2014, 08:20

... List of Figures and Table LIST OF FIGURES AND TABLES FIGURES Chapter PAGE Figure Life cycle of Drosophila melanogaster Figure Regulation of stem cell division Figure Asymmetric neural stem cell ... asymmetric cell division can upset homeostasis of stem cell self-renewal causing neural stem cells to turn into tumor-initiating cells that recapitulate several hallmarks typical of mammalian ... for asymmetric localization of cell fate determinants 16 1.5 Stem cells and cancer – the cancer stem cell hypothesis 20 1.6 Link between failure in stem cell asymmetric division and...

Ngày tải lên: 11/09/2015, 14:32

... potential of pluripotent stem cells to respond to adult specific signals Following the recent demonstration of the ability of the ECM from acellular lung to drive the differentiation of ES cell- derived ... kidney progenitor cells before being applied to the acellular organ Keywords Kidney Extracellular matrix Pluripotent cells Stem cells Organ culture Decellularized kidney Six2 + Acellular Introduction ... kidney cells supported by the extracellular matrix (ECM) of an adult rat acellular kidney resulted in the restoration of kidney function [1] This proof of principle study set the stage for using acellular...

Ngày tải lên: 19/11/2022, 11:43

... upregulated in BMP2+ cells Long-term differentiation of the BMP2+ cells resulted in neural crest stem cells (NCSCs), smooth muscle cells, epithelial-like cells, neuronal-like cells, osteoblasts ... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... progenitors Enrichment of the neural crest cells and their lineages by BMP2 promoter-driven selection markers paves the way for ES cell- derived neural crest stem cell biology These cells are attractive...

Ngày tải lên: 14/08/2014, 08:20

... Genetics, Department of Evolutionary Genetics, University of Cologne, Zülpicher Str., 50674 Cologne, Germany comment Global transcriptome analysis of murine embryonic stem cell- derived cardiomyocytes ... in the cardiomyocytes derived from embryonic stem cells reflects the biologic, physiologic, and functional processes that take place in mature cardiomyocytes Identification of cardiomyocyte-specific ... prerequisite for future cell replacement therapies in heart failure [1,2] Recently, genetic strategies for differentiation of stem cells and nonmuscle cells through expression of developmental control...

Ngày tải lên: 14/08/2014, 20:22

... for “stemness” using a candidate gene approach was adopted ES cells are derived from the ICM of blastocysts and are similar (although not completely identical) to the pluripotent cells of the ... for a role in ES cell self renewal Of primary interest are genes whose expressions are restricted to the embryonic stages, as pluripotency is characteristic of embryo derived stem cells The similarity ... commitment of ES cells This baffles the interpretation of the roles of the various pathways and pathway components in ES cells, and hinders their applications In vivo hypomorphic alleles studies of Nodal...

Ngày tải lên: 11/09/2015, 16:02

... ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For transfection of ... day of selection 4.9 Alkaline phosphatase staining Detection of alkaline phosphatase, which is indicative of the nondifferentiated state of ES cells, was carried out using a commercial ES Cell ... 4.14.1 Enhancement of Nodal/Activin signaling E14, TAG1 or tet-Nodal E14tg2a cells were seeded in ES cell medium at a density of 676 cells/cm2 without gelatin The next day, the seeded cells were subjected...

Ngày tải lên: 11/09/2015, 16:02

... pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells ... fails to maintain self-renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and Benvenisty, N (2006) Overexpression of NANOG in human ES cells enables feeder-free growth ... Smith, A (2003) Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells Cell 113, 643-655 Chambers, I., and Smith, A (2004) Self-renewal of teratocarcinoma...

Ngày tải lên: 11/09/2015, 16:02

... mechanism underlying self renewal and cell fate determination of ES cells 1.2 Molecular basis underlying ES cell self renewal Despite the importance of stem cell self renewal, we are only beginning ... determinant of the ability of feeders to support ES cell self renewal Today, most investigators culture mouse ES cells feeder free in the presence of serum with supplementation of LIF LIF is a member of ... ES cells for the study of pluripotency and pluripotent cell self renewal Scientists have recently reported the successful reprogramming of mouse and human somatic cells into pluripotent stem cells,...

Ngày tải lên: 11/09/2015, 16:02

... Subunit of Cadherin protein complex Intracellular mediator of canonical Wnt signaling Non-SET domain protein Histone H3 methyltransferase Transcription factor Intracellular mediator of canonical ... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells and differentiated cells...

Ngày tải lên: 11/09/2015, 16:03

... a variety of neural stem cells with varying degrees of commitment remain in the culture while the CX cells derived from a single initial cell would only represent one subtype of stem cell Thus, ... A: Long-term molecular and cellular stability of human neural stem cell lines Exp Cell Res 2004, 294(2):559-570 Takahashi K, Yamanaka S: Induction of pluripotent stem cells from mouse embryonic ... development of immortal cell lines to provide suitable cells in large enough numbers as and when required is an important aim The cells of choice for this purpose are human neural stem cells (hNSCs),...

Ngày tải lên: 01/11/2022, 09:54

... has been shown that embryonic stem cells (ESCs), Muller cells, mesenchymal stem cells, and some other cells can be induced to develop into RPCs or photoreceptor cells6–10 Several studies have ... adopted a culture system that could harvest a substantial quantity of retinal progenitor cells (RPCs) from human embryonic stem cells (hESCs) within a relatively short period of time Furthermore, ... January 2017 Published: 13 February 2017 Experimental Study of the Biological Properties of Human Embryonic Stem Cell? ? ?Derived Retinal Progenitor Cells Jingzhi Shao1, Peng-Yi Zhou1 & Guang-Hua Peng1,2...

Ngày tải lên: 24/11/2022, 17:42

... mesenchymal stem cell- derived extracellular vesicles have significant age-dependent differences in their immune profiles The promising role of mesenchymal stem cells (MSCs), whose mechanism of action ... Characterization of mesenchymal stem cell- derived extracellular vesicles (A) Number of particles per cell at different age groups by the NTA assay (B) Concentration of protein per cell at different ... because of their relationship with the pathway of interest Since miR-21-5p showed the highest statistically significant expression in extracellular vesicles from mesenchymal stem cells of the...

Ngày tải lên: 24/11/2022, 17:48

... hPSCHPC, hPSC -derived HPC; HPC-HBC, HPC -derived hepatoblast-like cell; HPC-HC, HPC -derived hepatocyte-like cell; hESC, human embryonic stem cell; iPSC, induced pluripotent stem cell; MEF, mouse ... every days 2.5 Assessment of cell viability The Alamar Blue assay was used to assess cell viability The cells were seeded on 96-well plates at a cell density of 1×104 cells and cultured for days ... Immunostaining images of hPSC-HPCs (derived from H9 cells) of tightly packed colony selected from hPSC-HLPCs cultured in HepSCF-2F medium Cells were stained with antibodies of hepatic stem markers and...

Ngày tải lên: 04/12/2022, 15:13

... adhesion of tumor cells to the extracellular matrix, the degradation of the extracellular matrix by the proteinase secreted by the tumor cells, and the transfer and proliferation of tumor cells[36]. ... could silence T cells in G0/G1 phase, which might be one of the possi- ble mechanisms of MSC’s inhibitory effect on T cells. When the inhibitory effect of CML -derived MSC on T cell proliferation ... ± S.D. of triplicates of five separate experiments with similar results. Activators of T cells were analyzed including CD25, CD69, and CD44. The activation of T cells was analyzed in T cells alone...

Ngày tải lên: 10/08/2014, 10:21

... Clonal analysis of the differentiation potential of human adipose -derived adult stem cells J Cell Physiol 2006, 206:229-237 Fukuda K: Reprogramming of bone marrow mesenchymal stem cells into cardiomyocytes ... analysis software (SABiosciences) To study the effect of viral exposure on HD cells, we compared the expression of selected genes in HD-HIV cells versus un-infected HD cells The gene profile of HD-HIV ... mesenchymal stem cells from the bone marrow of rhesus macaques Stem Cells Dev 2005, 14:440-451 doi:10.1186/1742-4690-8-3 Cite this article as: Nazari-Shafti et al.: Mesenchymal stem cell derived...

Ngày tải lên: 13/08/2014, 01:20

... Characterization of tocotrienol analogs in TAMH cells 104 4.2.1 Cytotoxicity of T3 analogs in TAMH cells 105 4.2.2 Cellular uptake of different concentration of T3 analogs in TAMH cells ... H2O2-induced on cell death in TAMH cells 112 4.3.2 Effect of lower dosage of -T3 and -T3 against APAP and H2O2-induced injury on cell viability in TAMH cells 118 4.3.3 Effect of -TP and ... Q, Lee CN, Mitchell W, et al: Elucidating the secretion proteome of human embryonic stem cell- derived mesenchymal stem cells Mol Cell Proteomics 2007, 6:1680-1689 Lai RC, Arslan F, Lee MM, Sze...

Ngày tải lên: 09/09/2015, 11:21

... understanding of role of cancer stem cells in tumour progression In addition, the same model of analysis can be applied to future studies in other cancer types to compare the roles of the cancer stem cell ... minority of cancer stem cells These cancer stem cells possess tumour-related features such as uncontrolled growth and the ability to form metastases They also maintain their inherent stem cell capacity ... Weissman IL Stem cells, cancer, and cancer stem cells Nature 2001; 414(6859): 105-11 Ricci-Vitiani L, Lombardi DG, Pilozzi E, et al Identification and expansion of human colon-cancer-initiating cells...

Ngày tải lên: 03/10/2015, 20:57

... INTRODUCTION 1.1 Spermatogonial Stem Cells (SSCs) Differentiation of germ cells in the testis originates from a constantly renewed small pool of stem cells, termed SSCs located at the basement membrane of the seminiferous ... diagram of spermatogonial multiplication and stem cell renewal. This scheme probably applies to all mammals except for humans (De Rooij 1983). Stem cells (As) proliferate, renewing the stem cell ... Cloning of Medaka glial cell- derived neurotrophic factor (GDNF) and its receptor GFRα1 LIM CHIAT KOO (B.Sc. (Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL...

Ngày tải lên: 03/10/2015, 20:57