functional consequences of thiamine deficiency
Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
Ngày tải lên :
16/03/2014, 10:20
... from the distal point of the rest of the protein Such a dipole will have weak interactions with the rest of the protein Thus, if turned off (by removal of Arg and protonation of Asp185), the protein ... of microgram quantities of protein utilizing the principle of proteindye binding Anal Biochem 72, 248254 30 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of ... reaction Analysis of the effect of the mutations on protein stability revealed three distinctive mechanisms of relaxation, and in the case of S36A, no relaxation at all The rst type of relaxation...
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Báo cáo y học: "Functional consequences of DECTIN-1 early stop codon polymorphism Y238X in rheumatoid arthritis" pptx
Ngày tải lên :
12/08/2014, 11:23
... in tissue sections of synovial lesions obtained from RA patients The functional consequences of the presence of the DECTIN-1 Y238X polymorphism on cytokine production capacity of macrophages were ... Biosystems) for an amount of 10 ng cDNA with SYBR Green Master mix Quantification of the PCR signals was performed by comparing the cycle threshold value (Ct) of the gene of interest of each sample with ... the functional consequences of the Y238X early stop codon polymorphism in DECTIN-1 have been studied in detail This polymorphism was demonstrated to result in a complete loss of function of the...
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Functional consequences of chromosomal rearrangements in neurodevelopmental disorder
Ngày tải lên :
09/09/2015, 11:21
... FUNCTIONAL CONSEQUENCES OF CHROMOSOMAL REARRANGEMENTS IN NEURODEVELOPMENTAL DISORDER KAGISTIA HANA UTAMI (M Sc) University Medical Center Utrecht A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... conservation of MED13L across vertebrate species 97 Figure 27 Expression profile of med13b in zebrafish embryo 99 Figure 28 Knockdown of med13b in zebrafish embryo 100 Figure 29 Eye size of ... subtypes of ASD in the DSM-IV manual ASD affects about in 110 individuals, with age of onset of three years old ASD is highly heritable compared to other types of NDDs, and the presentation of ASD...
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Electrophysiological study to explore the functional consequences of ng expression on neuroblastoma cells (n2a)
Ngày tải lên :
30/09/2015, 06:42
... reduction of Ng in the brain While the redox state of Ng, similar to the 13 state of phosphorylation of Ng, may regulate the level of CaM, which in turn of course modulates the activities of CaM-dependent ... isoform of PKC after stimulation of the metabotropic glutamate receptor It is more likely that Ng is a PKC-γ substrate in vivo An important question is: what are the functional consequences of ... increased the rate of dissociation of Ca2+ from the Cdomain of CaM when bound to intact CaM-dependent protein kinase II Thus, PEP-19, and presumably similar members of the IQ family of proteins, such...
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt
Ngày tải lên :
19/02/2014, 02:20
... quantitative strategy to offset the lack or excess of kinetic energy (as a measure of temperature) and its effect on enzyme activity Alternatively, we know of a number of examples of qualitative strategies, ... confirmed the existence of two LDH-A mRNAs of different lengths: short (a-isoform; b mRNAa ldhÀa ¼ 1332 bp) and long (b-isoform; mRNAldhÀa ¼ 1550 bp) Sequence analysis of these mRNAs has shown ... of the LDH gene has been found Sequence divergence between pairs of isoforms, known in diploid teleosts is $ 20–25% (for ORFs of mlc1 and mlc2 genes), while divergence between the two forms of...
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Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot
Ngày tải lên :
16/03/2014, 13:20
... dissociation was observed with the A8B8 isoform of GAPDH leading to the A2B2 isoform with a higher NADPHdependent activity [39] By analogy with the CTE of the isoform of GAPDH [15,37], we hypothesize ... changes in activities of the enzymes is thus of crucial importance to find a general mode of light regulation of the Calvin cycle in all photosynthetic organisms GAPDH is composed of four identical ... et al on the A2B2 isoform of GAPDH [24,37], formation of a disulfide bridge in the oxidized CTE prevents the interaction between the 2¢-phosphate of NADPH and the side chain of Arg77 (spinach numbering,...
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Báo cáo khoa học: "Consequences of an excess Al and a deficiency in Ca and Mg for stomatal functioning and net carbon assimilation of beech leaves" ppt
Ngày tải lên :
08/08/2014, 14:22
... conductance and net carbon assimilation of beech seedlings This reduction of stomatal aperture is the result of limited accumulation of K + , and may be of organic osmoticum, in the guard cell ... and a deficiency in Ca and Mg RESULTS 3.1 Element concentrations of leaf cells The distribution of Al in the different leaf cells is presented in figure In control plants, a concentration of 0.94 ... sufficient amount of free calcium in the vicinity of the guard cells and ii) the existence of a Ca independent signal transduction pathway for these tree species The occurrence of several transduction...
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Báo cáo khoa học: " Consequences of hazardous dietary calcium deficiency for fattening bulls" pdf
Ngày tải lên :
12/08/2014, 18:21
... macroscopic findings of both front and hind limbs of the three groups of dairy bulls At the time of slaughter, animals were clinically healthy The first group of animals consisted of 20 bulls slaughtered ... Number of individual bones/legs, n* = Number of affected animals (animals with one or more osteoarthritic lesions graded ≥ 2) S = Sensitivity of examination of individual bones/combination of bones/ ... had different degrees of swelling of the joints, mainly in the hock and stifle joints The unit where leg problems emerged was for fattening of dairy bulls from the age of months to slaughter...
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Báo cáo y học: "Natural History and Clinical Consequences of Hepatitis B Virus Infection"
Ngày tải lên :
02/11/2012, 11:17
... B Age at the time of infection is a strong determinant of chronicity, the earlier the acquisition of infection, the higher probability of developing chronic infection Levels of viremia in chronic ... incidence of hepatic decompensation was 16%, the incidence per 100 person years was 3.3 and the mean interval between the time of diagnosis of cirrhosis and the onset of first episode of decompensation ... primary infections are self-limited with clearance of virus and development of immunity However, an estimated 3% to 5% of adults and up to 95% of children develop chronic HBV infection Persistent...
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CONSEQUENCES OF THE NEOLITHIC
Ngày tải lên :
01/11/2013, 11:20
... Feast and harvesters of crops, as well as caretakers of the home and caregivers to the children.4 Men, previously hunters, were now often herdsmen, providing more in the way of leisure time to ... around 3000 BCE, at the beginning of the Bronze Age, that human society emerged into the light of recorded history – having accomplished the domestication of most of the vegetables, fruits, and ... those foods But clearing the land for the cultivation of grasses and tubers and the herding of animals brought a whole train of ecological consequences to the planet Plant and animal species were...
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Tài liệu Functional Specification of JPEG Decompression. and an Implementation for Free ppt
Ngày tải lên :
09/12/2013, 15:15
... bene ts of abstraction in a functional language This article assumes no knowledge of JPEG or any of its algorithms, nor of specialized functional programming techniques Basic knowledge of functional ... prelude of most functional languages Six functions of general nature that we need are not de ned in the Gofer prelude They are de ned in this section, and may also serve to get used to the Gofer ... elements of a matrix, a function matconcat which collapses a matrix of sub-matrices into one big matrix, and a function matzip which transforms a list of matrices into a matrix of lists of corresponding...
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Tài liệu The long-term reproductive health consequences of female genital cutting in rural Gambia: a community-based survey doc
Ngày tải lên :
13/02/2014, 16:20
... sealed had no signs of closure Of the three main ethnic groups, 98% of Mandinkas, 4% of Wollofs and 32% of Fulas had signs of genital cutting The socio-demographic characteristics of cut and uncut ... 643±653 august 2001 L Morison et al Long-term consequences of FGC in The Gambia secondary level education (3% of Mandinkas, 6% of Fulas and 1% of Wollofs) During interviews with a ®eldworker 58% ... Three of these women reported being `sealed' (WHO type III) Of the 1157 women who were examined by a gynaecologist 668 (58%) had signs of genital cutting The frequency of the different types of...
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Tài liệu Physical health and functional ability of an elderly, population in Sri, Lanka doc
Ngày tải lên :
14/02/2014, 06:20
... problems with PADL 12.32 13.43 7.81 8.33 Years of LE free of problems with all ADL % of total LE free of problems with PADL 93.3 91.2 % of total LE free of problems with all ADL 59.2 56.6 shown that ... expectancy free of problems with ADL activities was relatively low for both genders (Table 8) Discussion Identification of health problems and functional ability of an elderly population is of importance ... member of the household present at the time of the interview was identified as an "informant" At each interview where an informant was present, assessment of the informant of the health status of...
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Tài liệu THE HEALTH CONSEQUENCES OF SMOKING pptx
Ngày tải lên :
14/02/2014, 13:20
... health consequences of smoking have been submitted: The Health Consequences General, 1972 of Smoking, A Report of the Surgeon The Health Consequences of Smoking, 1973 The Health Consequences of Smoking, ... M.D - Professor, Department of Epidemiology, Harvard School of Public Health, Harvard University, Boston, MA and Chief of Pulmonary McLean, Ross, M.D - Professor of Medicine Gray School of Medicine, ... M.D., F.R.C.P - Professor, Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, Ml Hoffmann, Dietrich, Ph.D - Member, and Chief, Division of Environmental Carcinogenesis,...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
Ngày tải lên :
15/02/2014, 01:20
... the metabolic consequences of having HPRT deficiency are an overproduction of uric acid and elevated levels of PRPP [3] Patients having mutations resulting in partial HPRT deficiency often suffer ... analysis of a wide range of species indicates that HPRT have eleven completely conserved amino acids, whereas PRTFDC1 genes not show full conservation of these eleven residues [10,11] Because one of ... distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino acids 145–149 (Fig 1C) A second Ca2+ ion is coordinated by the side chain of...
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Tài liệu Consequences of Voluntary and Mandatory Fair Value Accounting: Evidence Surrounding IFRS Adoption in the EU Real Estate Industry docx
Ngày tải lên :
18/02/2014, 01:20
... Adopting a Label: Heterogeneity in the Economic Consequences of IFRS Adoptions Working paper, University of Frankfurt, University of Pennsylvania, University of Chicago, and MIT Daske, H., L Hail, C ... 2000 The Economic Consequences of Increased Disclosure Journal of Accounting Research 38 (Supplement): 91-124 27 Muller, K 1999 An Examination of the Voluntary Recognition of Acquired Brand Names ... suggesting adoption of IAS 40 should play a critical role in “leveling the playing field” by requiring provision of previously unknown fair values of these core assets for a subset of firms This leads...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
Ngày tải lên :
18/02/2014, 06:20
... formation of detectable products Figure S4 shows that conversion of 2,4-pentanedione (5 mm) proceeded with depletion of a molar equivalent of dissolved O2 HPLC analysis of the products of the enzymatic ... of the 4p orbital with the 3d orbital of the metal cofactor Two important pieces of information can be gleaned from the pre-edge peak First, occurrence of this transition implies distortion of ... superoxide in the course of the reaction The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c This was validated by adding a total of 2.5 U of commercial SOD...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
Ngày tải lên :
18/02/2014, 14:20
... the dynamics and features of this protein Role of MCPIP in regulation of the endogenous IL-1b transcript level After identification of a PIN domain in the MCPIP by use of bioinformatics, we decided ... and also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP and the level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA ... transcript independently of the presence of AREs (Fig 6B, lanes and 5) It is possible that in the region located before the AREs, consisting of 343 bp of the 3¢ UTR of the IL-1b transcript, there...
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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt
Ngày tải lên :
18/02/2014, 16:20
... ratio of UpUMPK ⁄ phosphate of 25 ⁄ The effect of orthophosphate on enzyme activity was examined It was shown that phosphate inhibited UpUMPK activity with an IC50 value of mm (Fig 7) Functional consequences ... Superposition of UpUMPK (green) on top of E coli UMPK (blue) at the active site (B) Binding of UMP to E coli UMPK and the location of amino acid residues in UpUMPK based on the superposition value of 0.7 ... structure of the UpUMPK was determined by ˚ X-ray crystallography to a resolution of 2.5 A with a final R value of 23.3% and Rfree of 28.5% (Table 1) The enzyme is a hexamer composed of three dimers...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
Ngày tải lên :
18/02/2014, 18:20
... Ca atoms of the catalytic triad, are positional homologs of the R148 and K300 residues of P furiosus archaeal primase, both of which are known to play a pivotal role in the activity of this protein ... side-chains of the first pair of residues, i.e K135 and R148, matched almost exactly; those of the second pair were in close proximity The R148 residue of the Pfu-primase is part of a motif which ... The ability of polymerases to use Mn2+ instead of Mg2+ as a required cofactor is well established [25] However, the biochemical properties of polymerases are altered as a result of replacing...
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