function migration survival and differentiation of schwann cell precursors

... 3T3.T4.CCR5 cells and cells expressing a soluble version of the gp120/gp41 of strain JR-FL (CHO JR-FL gp160, clone A19) were obtained from the NIH AIDS Research and Reference Reagent Program, Division of ... of the ligand to both cell types was observed (Fig 2A) Some binding of radioactive MIP-1a to nontransfected CHO cells, even in the presence of excess cold Fig CCR5-myc-his expression in CHO cells ... His6-tagged version of CD4, CD4-his, as described in Materials and methods After cloning and selection by FACS analysis (not shown) of cells coexpressing CCR5 and CD4, sincytium formation with cells expressing...

Ngày tải lên: 08/03/2014, 09:20

... Differential effects of ligand-activated RARa and RXRa on the promoter activity of the hGnRH II gene in TE671 and JEG-3 cells In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on ... activity of hGnRH II gene in JEG-3 cells (Fig 7) Differential effects of ligand-activated RARa and RXRa on the promoter activity of the hGnRH II gene in TE671 and JEG-3 cells Over-expression of RARs ... regulation of GnRH II gene by RARs, TE671 and JEG-3 cells were treated with all-trans retinoic acid (ATRA; a ligand of RARs) and ⁄ or 9-cisRA (a ligand of RXRs) before the measurement of GnRH II...

Ngày tải lên: 16/03/2014, 10:20

... ABCG5 and ABCG8 form obligate heterodimers that are expressed in the apical membrane of enterocytes and in the canicular membrane of hepatocytes [15] They limit the absorption of plant sterols and ... disrupted function of ABCG5 and ABCG8 in humans or mice, it was recently shown that sterols are the direct substrates of ABCG5 and ABCG8 Inside-out membrane vesicles prepared from Sf9 insect cells ... wide range of structurally and functionally diverse substrates such as chemotherapeutics, antibiotics and HMG-CoA reductase inhibitors Yet, physiological substrates and the roles of ABCG2 in...

Ngày tải lên: 22/03/2014, 15:21

... diverse cellular functions, including cell- cell interactions, cell adhesion, cell aggregation, cell migration, cell cycle progression, differentiation, inflammation, angiogenesis, and maintenance of ... essential for cell proliferation, migration and differentiation of cells in early development of embryos [149] This protein has EGF-like extracellular domains crucial for rolling up and adhesion of endothelial ... VWF include cell adhesion, cell migration, cell cycle progression and differentiation of endothelial cells [175-178] The VWF also acts as a permeability barrier for endothelial cells and is vital...

Ngày tải lên: 18/06/2014, 15:20

... role of the receptors in signaling, loss -of -function mutations were initially isolated in four out of the five Arabidopsis ethylene receptors, no mutation being isolated for ERS1 [13] Loss -of -function ... Here we report on the isolation and analysis of two new T-DNA insertion mutant alleles of ETR1 and ERS1, named etr1-9 and ers1-3 Our results indicate that both etr1-9 and ers1-3 are null alleles ... inhibition of root growth and decreased production of leaves from the axillary meristems of etr1-7;ers1-2, so that the overall morphology of the plant appeared more similar to that of etr19;ers1-3...

Ngày tải lên: 12/08/2014, 05:20

... pressure of the muscle cell, which allows the extracellular water to 65 move into the cell and increase the cell volume (Kingston & Bayly 1998) Interestingly, recent studies have suggested that cell ... Regulation of pH in the muscle cell Several mechanisms in the muscle cell operate to prevent acidification and its consequences (Fig 3) The most important of these are buffer capacity and export of lactic ... capillarization and enzymatic profile of locomotor and nonlocomotor muscles of horses and steers Acta anat 1994, 151, 97-106 Kingston JK, Bayly WM: Effect of exercise on acidbase status of horses Vet...

Ngày tải lên: 12/08/2014, 15:20

... of EP1, EP4 and of a variant of EP3 is mediated by increase of intracellular cAMP [28,29] In inflammatory cells, this phenomenon is associated with an inhibition of effector's cell functions such ... Finally, the role of prostaglandins in physiology and immune system is determined by multiple factors such as cellular context, receptor expression profile, receptor-ligand affinity and differential ... 300 nM of primer pairs and a set of primers specific for this hIL-5Rα isoform [16] PCR protocol consisted of cycle at 95°C for 10 minutes followed by 40 cycles at 95°C for 30 seconds and at 64°C...

Ngày tải lên: 12/08/2014, 16:20

... negative and positive cells were clearly seen and positive cells were better comparable after counterstaining with hematoxylin Classification of positively stained cells The evaluation of ERα and ... gland Using a LBA, ERs were found in the adrenal gland of the rat [4] Further, using IHC, ERs were found to be localised within cell nuclei of the adrenal cortex of both rhesus monkey [5] and ... staining was observed in cell nuclei of all compartments of the endometrium (surface epithelium, glandular epithelium and connective tissue stroma) and myometrium (see Table and Fig 1) For all mares,...

Ngày tải lên: 12/08/2014, 18:22

... control of cell wall assembly and as a determinant of monovalent cation and alkaline pH tolerance [63-65] Although conclusive evidence on the functional relationship between activated Rim101 and cell ... and cadmium/nickel toxicity by testing the effect of increasing iron concentrations on the fitness of cells lacking either subunit of Fet3/Ftr1 (deletion of which causes a genetic surrogate of ... alleviating effect of iron supplementation on cadmium toxicity, in both WT and fet3Δ cells: competition between the two metals at the level of cellular uptake; and downregulation of promiscuous (iron/cadmium)...

Ngày tải lên: 14/08/2014, 08:21

... SKW6.4 and H9 cells 67 Fig 3.5 Apoptotic effects of adenosine on SY5Y and MN9D cells 69 Fig 3.6 Apoptotic effects of CCPA on SY5Y and MN9D cells 70 Fig 3.7 Apoptotic effects of CGS21680 on SY5Y and ... Family of nucleoside transporters 23 Table Effects of extracellular purines on cell growth 38 Table Strategies of the extracellular mechanism studies of AIA in BHK 184 cells Table Strategies of the ... the hyperpolarization and depolarization of mitochondria, translocation of Bax and cytochrome c, elevation of cytosolic Ca2+ level, cellular acidification and activation of caspases An intrinsic...

Ngày tải lên: 11/09/2015, 16:07

... Characterization of the binding of PCSK9 to intact cells S A Mousavi et al Fig Dissociation time courses and effects of unlabeled ligands and low pH on the dissociation rates of [125I]TC-PCSK9 (A) and [125I]TC-PCSK9-D374Y ... or with unlabeled ligand or medium (pH 6.2), and dissociation of specifically bound ligands was followed as a function of time Data are presented as percentage of total ligand bound at zero time ... HepG2 cells are able to internalize and degrade [125I]TC-PCSK9 at 37 °C However, the interaction of ligands with cell surface receptors at 37 °C is a function not only of the rate constants of association...

Ngày tải lên: 14/02/2014, 14:20

... A summary of the 20E inducibility of the transcripts analyzed is presented in Table The A aegypti ecdysone receptor Two EcR isoforms (AaEcRA and AaEcRB) and two USP isoforms (AaUSP-A and AaUSP-B) ... established a lack of fluctuation in the protein levels of AaEcR and AaUSP isoforms during vitellogenesis [13], suggest a lack of significant transcriptional and translational regulation of the two components ... orthologs of D melanogaster, An gambiae and Ap mellifera NRs, and one which is a likely ortholog of the Ap mellifera and T castaneum PNR-like NR [20–22] that is also present in the genome of An gambiae...

Ngày tải lên: 18/02/2014, 13:20

... barrel between the b3-strand and the a3-helix; (b) the catalytic machinery consists of the b4-strand aspartate (nucleophile), b5-strand glutamate (proton donor) and b7-strand aspartate (transition-state ... rBATs and GH13 enzymes is the lack of domain B protruding out of the TIM barrel in the place of loop connecting the b3-strand to the a3-helix [9,21] Sharing domain B by rBATs and GH13 enzymes, and ... Some of these features can be traced in the sequences of hcHAT1 and hcHAT2 groups as well as of the ATG proteins (Fig 2), indicating evolutionary relationships of all these enzymes and proteins and...

Ngày tải lên: 18/02/2014, 13:20

... radioligand This radioligand had iodinated tyrosines at positions 21 and 27 and a specific activity of 4000 CiÆmmol)1 Saturation experiments were carried out with serial dilutions of radioligand, and ... BIIE0246 and cPP, with pKi values of 6.56 ± 0.50, 5.68 ± 0.22 and < 6.0 (Table 1) No 2052 Fig Alignment of Y7 and Y2 sequences Amino acid alignment of the Y7 sequences from chicken and zebrafish ... separation of the lineages leading to birds and mammals Discussion Fig Phylogenetic tree of Y7 and Y2 sequences Phylogenetic tree of the Y2 subfamily of receptors based on the entire coding region of...

Ngày tải lên: 19/02/2014, 07:20

... as described above for whole cells and cell membranes When the effects of ligands were analysed, they were preincubated with the cells for 15 prior to the addition of the antibodies The energy ... expression of HIV- and FLAG-tagged D2L and D2S receptors in Sf9 cells To achieve this, mAbs directed against gp120 (clone 11/4C) and the FLAG sequence were used, and Fig shows the band pattern ... (Sf9) and HEK293 cells Binding of Eu3+-anti-FLAG Ig (2.5 nM) was carried out on whole Sf9 cells or on Sf9 cell membranes expressing different D2 receptor isoforms (A), or on whole HEK293 cells...

Ngày tải lên: 21/02/2014, 00:20

... spectra of snake neurotoxins The spectrum of sWntx-5 was monitored between 190 nm and 250 nm, in the presence of nitrogen The cell path-length and temperature of measurement were 0.05 mm and 20 ... purity and identity of the sWntx-5 Circular dichroic spectrum of sWntx-5 As shown in Fig 4, the far UV spectrum of the sWntx-5 displayed a positive band at 196 nm and a broad negative band at ... degree of identity of the sequences isolated from cobras is striking, both in terms of length and amino acid distribution Those from kraits, mambas and coral snakes display more deviations and a...

Ngày tải lên: 21/02/2014, 03:20

... glycosylation site of DmUGT Fig Expression of DmNST/DmUGT in Lec2 and Lec8 cells (A) Lec2 and Lec8 cells were transfected with appropriate plasmids as speci®ed below, and CST and UGT activities of cDNA ... (lanes and 2) and Lec8 (lanes and 4) cells Cell extracts were prepared from cells transfected with appropriate plasmids as speci®ed below, and were subjected to Western blot analysis Lanes and 3, ... (panels i±p) cells Lec2 cells were stained with FITC-labeled PNA, and Lec8 cells with FITC-labeled GS-II to assess CST and UGT activities of the transporters and mutants The expression of proteins...

Ngày tải lên: 21/02/2014, 15:20

... clonal expansion of T-cells bearing human Vb3, Vb12, Vb17 and Vb19 [20] Others showed proliferation of human T-cells bearing Vb1, Vb2, Vb3, Vb5.2, Vb12, Vb15 and Vb17 domains and found differences ... pathologies and because of the potential use of the toxins as biological weapons Alteration of their MHC and TCR binding capacity by site directed mutagenesis could be useful in the development of vaccines ... hVb2.1hCb2 and hVb1hCb2 (genes kindly provided by U Utz and R P Sekaly, University of Montreal, Canada), were cloned between the NdeI and EcoRI restriction sites of the pET17b expression vector and...

Ngày tải lên: 07/03/2014, 16:20

... al Results Construction of truncated and point mutated B2wts and B1 ⁄ B2 receptor chimeras Several studies with truncations of, and deletions in, the C-terminal part of B2wt have demonstrated ... min) that was comparable to that of B1CB2 and B1YB2 (Fig 2B) Agonist-induced internalization of modified B1KB2 constructs Phosphorylation patterns of B2wt and of B1 ⁄ B2 chimeras reflect their ... in Role of helix and C-termini in bradykinin receptors receptor signaling because: (a) interruption of the amphipathic structure of B1wt helix in B1wt and B1KB2 through the presence of a serine...

Ngày tải lên: 07/03/2014, 16:20