from aquafeed to marine cells

APPLICATIONS OF IMMUNOCYTOCHEMISTRY pot

APPLICATIONS OF IMMUNOCYTOCHEMISTRY pot

... to 75% of the perikaryon of some neurons in the aged brain (Terman & Brunk, 1998) Lipofuscin in neurons appears to be derived primarily from the breakdown of autophagocytosed mitochondria into ... located in the mitochondria Mitochondria can also be visualized with the use of mtPericam that labels the mitochondria green after transfecting into cells In addition, mtPericam, due to its Ca2+ sensitivity, ... Histochemistry and Cytochemistry, Vol 49, No 8, (August 2001), pp 931-937, ISSN 0022-1554 Spector, D.L & Goldman, R.D (Eds.) (2006) Basic methods in Microscopy: Protocols and Concepts from Cells: ...

Ngày tải lên: 28/06/2014, 08:20

330 233 1
Tài liệu Báo cáo khoa học: Post-translational modifications of the linker histone variants and their association with cell mechanisms docx

Tài liệu Báo cáo khoa học: Post-translational modifications of the linker histone variants and their association with cell mechanisms docx

... enriched in growing cells by a factor of seven as compared with starved cells Similarly, phosphorylated Thr35 was also found to be enriched by a factor of four in growing cells It is perhaps ... linker histones C Wood et al Table PTMs that uniquely identify human MCF7 from HeLa cells ‘+’ indicates a modification that is present on MCF7 linker histones but is missing from HeLa linker histones ... that the cells are in mitosis The H1.3 variant in Garcia et al [42] was found to be phosphorylated at Ser189, Thr147, PTMs of linker histones Table Phosphorylations of the human linker histones...

Ngày tải lên: 18/02/2014, 08:20

13 633 0
báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt

báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt

... Fluor 750, to label human hepatic stellate cells to monitor the engraftment process in vivo [36] Furthermore, VivoTag 680-conjugated iron oxide particles have been intravenously injected into mice ... recent study, DMSA-coated Fe2O3 nanoparticles were shown to have a low cytotoxicity [57], and have been used to label a variety of mammalian cells [47-49,55] Conversely, DMSA-coated iron nanoparticles ... often been used to investigate the intracellular distribution of magnetic nanoparticles in cells [17,18,26]; however, these nanoparticles cannot be applied to in vivo imaging as the autofluorescence...

Ngày tải lên: 11/08/2014, 00:22

14 400 0
Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray

Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray

... buffer, pH 7.4 to a stock concentration of 1mg/ml These were spotted onto the thioester functionalized glass slides in various concentrations using an ESI SMATM arrayer (Toronto, Canada) For ... non-radiative phenomenon (no photons are emitted from the donor) in which energy from the donor (shorter wavelength emission) fluorophore is transferred to the acceptor (longer wavelength emission) ... (reduced in total light output) The acceptor’s emission is triggered by energy absorbance or transfer from the donor and results in a wavelength appropriate emission from the acceptor This transfer...

Ngày tải lên: 08/11/2015, 16:30

77 200 0
Aspects of carbohydrate quality and their relevance for risk markers of type 2 diabetes and related health outcomes

Aspects of carbohydrate quality and their relevance for risk markers of type 2 diabetes and related health outcomes

... the T2D cases [68] With a total diabetes prevalence of 0.84% (T1D and T2D together) this data suggests an increase compared to data from NHANES III (1988 to 1994), when a total prevalence of 0.41% ... Nonalcoholic Steatohepatitis NFκB Nuclear factor κB NHANES National Health and Nutrition Examination Survey OA Original Article OGTT Oral glucose tolerance test PAI-1 Plasmingen activator inhibitor type ... intake of 55% to 75% of total daily energy from carbohydrates was questioned and it was emphasized that the nature of dietary carbohydrates appears to be more important than the total intake [22]...

Ngày tải lên: 25/11/2015, 14:52

203 432 0
GROWTH PERFORMANCE AND SPERM QUALITY OF STRESS NEGATIVE PIÉTRAIN BOARS AND THEIR HYBRIDS WITH DUROC

GROWTH PERFORMANCE AND SPERM QUALITY OF STRESS NEGATIVE PIÉTRAIN BOARS AND THEIR HYBRIDS WITH DUROC

... Belgique (1999): A total of 41 boars, genetically identified at the halothane locus using PCR on samples from tail tissues according to the method of Fujii et al (1991), were divided into different ... time and same place as X Sperm quality traits were recorded from January 2010 to November 2012 A total of 328 ejaculates were collected from 25 boars, boars Đỗ Đức Lực, Hà Xuân Bộ, Farnir Frédéric, ... using ejaculation volume (VOL), spermatozoon motility (MO), sperm concentration (CO) and total number of spermatozoon in ejaculate (NT), rate of abnormal spermatozoon (R) and pH of semen (pH) VOL...

Ngày tải lên: 28/08/2013, 11:59

6 734 0
Characteristics of Leachate from Citrus Groves and their Changes in the Collecting Reservoirs in Matsuyama, Japan

Characteristics of Leachate from Citrus Groves and their Changes in the Collecting Reservoirs in Matsuyama, Japan

... pollutants from this region into the Seto Inland Sea Water quality in the small reservoirs also seems to have been affected However, field surveys about the runoff characteristics from citrus ... that M-alkalinity increased from 0.8 meq/L at St.4 to 1.8 meq/L at the reservoir, and the pH ranged from 7.1 to 10.3 at the reservoir The ratio of alkalinity increase to nitrate removal is in the ... of 0.002 to 0.46 mg-P/L The main component of phosphorus was phosphate, and the mechanisms of the phenomena were considered to be biological uptake and transition to the bottom due to the increase...

Ngày tải lên: 05/09/2013, 10:17

10 717 0
A study of the linguistic features of suggestion verbs in english and their vietnamese equivalents

A study of the linguistic features of suggestion verbs in english and their vietnamese equivalents

... (1) to say something in order to obtain an answer or some information; (2) to request (someone) to or give something; (3) to request permission to something;(4) to request to speak to; (5) to ... expect someone to something; (5) to wish to have; (6) to demand or order something SUGGEST refers to the meanings (1) to propose a plan or idea for someone to discuss or consider; (2) to put forward ... Equivalents - To say something in order to obtain an answer H i or some information - To request (someone) to or give something Yêu c u, k - To request permission to something H i ASK - To request to speak...

Ngày tải lên: 26/11/2013, 13:17

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A study of semantic and syntactic features of english famous love sayings and their vietnamese translation

A study of semantic and syntactic features of english famous love sayings and their vietnamese translation

... different from the usual meaning, in order to create a particular mental picture.” [12] Language originates from life and it reflects life It is used to depict our life, from the general things to the ... need to set the theoretical basic for this thesis to be carried out 3.4 DATA ANALYSIS After collecting 200 EFLSs and their 200 Vietnamese translation from books, dictionaries or websites , we catogorize ... translators of English may encounter a lot of difficulties in their translation process and they, as a result could commit unspected error In order to solve the problem, I would like to offer to...

Ngày tải lên: 26/11/2013, 13:21

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Tài liệu an analySiS of euro area Sovereign CDS anD their relation With government bonds docx

Tài liệu an analySiS of euro area Sovereign CDS anD their relation With government bonds docx

... strongly linked to common factors some of which proxy for changes in investor risk appetite Due to sizeable risk premia in CDS quotes changes in credit and non-credit-related components lead to different ... ratio of debt to its assets is a major risk factor This risk factor is also acknowledged in a fiscal policy perspective as the EU’s Stability and Growth Pact aims to cap a country’s total debt at ... interpolation to obtain weekly observations ECB Working Paper Series No 1271 December 2010 corporate spreads is more strongly linked to idiosyncratic stock price volatility than to aggregate stock price...

Ngày tải lên: 16/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

... the mitochondrial matrix and to be important to several NADPH-requisite mitochondrial processes, e.g resistance to a broad range of oxidative stress, respiration, arginine biosynthesis, mitochondrial ... indicating that Pos5p is a critical contributor to mitochondrial functions [2,3] We found that utr1pos5 and yef1pos5 cells appeared to grow somewhat less than pos5 cells on solid medium lacking arginine, ... in the cytosol Collectively, we assume that cytosolic CgLeu2p has the ability to utilize NADP and that it supplies cytosolic NADPH, whereas cytosolic ScLeu2p cannot provide NADPH due to its specificity...

Ngày tải lên: 20/02/2014, 01:20

13 560 0
Tài liệu Báo cáo khoa học: Localization of N-linked carbohydrate chains in glycoprotein ZPA of the bovine egg zona pellucida pptx

Tài liệu Báo cáo khoa học: Localization of N-linked carbohydrate chains in glycoprotein ZPA of the bovine egg zona pellucida pptx

... subjected to SDS/PAGE To detect the shift of the 21 kDa band, 2.5 mU of N-glycanase was added to lg (56 pmol) of ZPA To detect the shift of the 63 kDa band, 0.25 mU of N-glycanase was added to lg ... into neutral and acidic fractions by using a TSK-gel DEAE-5PW HPLC column (7.5 · 75 mm; Tosoh, Tokyo, Japan) equilibrated with NH3/H2O (pH 9.0) Elution was performed by a linear gradient from to ... further chromatographed on a size-fractionation HPLC column of TSK gel Amide-80 (4.6 · 250 mm; Tosoh) Elution was performed by a linear gradient decrease in acetonitrile from 65 to 50% in 0.5...

Ngày tải lên: 21/02/2014, 03:20

10 514 0
Tài liệu An International Comparison of Milk Supply Control Programs and Their Impacts pot

Tài liệu An International Comparison of Milk Supply Control Programs and Their Impacts pot

... operations need to be able to exit the system while efficient and new farmers need to be able to grow In the EU, milk quota was originally attached to land, so that the land needed to be bought ... circumstances, for the farmer to try to spread those fixed costs over as many cows as possible to average down his total cost It’s not unusual for farms in the US to be stocked at 110% of planned ... increased their share to nearly 20% in 2008 before falling back to 14% in 2009 New Zealand has taken the top spot from the EU-15, increasing their share from 30% in 1996 to 38% in 2009 Demand...

Ngày tải lên: 22/02/2014, 05:20

83 436 0
Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx

Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx

... cuticular proteins from other arthropods have not yet been characterized to the same extent as those from insects, a sequence motif similar to R & R consensus has been identified in proteins from an arachnid, ... to 10 [25] Their isoelectric points range from 6.5 to 9.2, and the 2D gel shows no protein spots in the acidic range from 3.5 to 5.5, corresponding to the members of acidic S and acidic DE in ... from the fly L cuprina (Peritrophin) [37], chitinase from the parasitic nematode Brugia malayi (Chitinase) [38], tachycitin from the horseshoe crab T tridentatus (Tachycitin) [15] and hevein from...

Ngày tải lên: 07/03/2014, 21:20

13 583 0
Báo cáo khoa học: Identification of microsomal rat liver carboxylesterases and their activity with retinyl palmitate potx

Báo cáo khoa học: Identification of microsomal rat liver carboxylesterases and their activity with retinyl palmitate potx

... from three separate preparations were combined and subjected to MonoQ chromatography Before MonoQ After MonoQ Total Total Specific Total Total Specific protein activity activity protein activity activity ... vitamin A trafficking and storage in specific liver cells will depend on the relative expression of isoenzymes in hepatocytes, stellate cells, Kupffer cells and endothelial cells The multiple tissue ... experimental samples was calculated from the fit of data to a standard curve by linear regression analysis The Km and kcat kinetic constants were derived from the fit of data to the Michaelis–Menten equation...

Ngày tải lên: 08/03/2014, 10:20

12 439 0
Polymer assisted synthesis of aligned amorphous silicon nanowires and their core shell structures with au nanoparticles

Polymer assisted synthesis of aligned amorphous silicon nanowires and their core shell structures with au nanoparticles

... be due to the much too growth rate (about 70 nm/s) We speculate the growth rate is so rapid that the Si atoms have no time to stack themselves into crystalline order Furthermore, due to high ... propose that the formation of the SiNWs follows the conversion from PPC to carbon particles, the reduction from native oxide layer to silicon monoxide, and the growth of SiNWs, which is schematically ... reveal some dandelion-like wires As is seen form the top view of the NWs film, the relatively straight nanowire splits into several curly sub-branches to different orientation The magnified image in the...

Ngày tải lên: 16/03/2014, 15:06

5 467 0
facile synthesis of porous a - fe2o3 nanorods and their application in ethanol sensors

facile synthesis of porous a - fe2o3 nanorods and their application in ethanol sensors

... placed through the tube to supply the operating temperatures from 100 to 500 °C Electrical contacts were made with two platinum wires attached to each gold electrode To improve their stability ... increased to 10 min, part of the nanoparticles began to combine with each other, and the rodlike structure appeared Upon prolonging the reaction time to 30 min, the products were totally transformed to ... layer leads to a decrease in the electron density on the sensor surface due to the transfer of electrons from the sensor surface to the adsorbate layer When the sensor is exposed to ethanol vapor,...

Ngày tải lên: 19/03/2014, 16:48

5 458 1
BENEFITS OF E-CRM FOR BANKS AND THEIR CUSTOMERS pdf

BENEFITS OF E-CRM FOR BANKS AND THEIR CUSTOMERS pdf

... customer to search on keywords to locate quickly specific answers on the Web site Mailing list, to receive more information, the visitor can add his/her e-mail address to a list to receive automated ... e-CRM benefits to bank 2.2 e-CRM benefits to Customers Frame of Reference 3.1 e-CRM benefits to bank 3.2 e-CRM benefits to Customers Methodology 4.1 Research Purpose 4.1.1 exploratory research ... forums allow visitors to share information with others and can help shape a Web site to serve effectively the customer needs A visitor/customer can post a message or can respond to a posted message...

Ngày tải lên: 22/03/2014, 21:20

73 710 0
Báo cáo khoa học: Identification of rice TUBBY-like genes and their evolution Qingpo Liu docx

Báo cáo khoa học: Identification of rice TUBBY-like genes and their evolution Qingpo Liu docx

... hypothalamic neurones, TUB was localized in the cytoplasm and nucleus, whereas, in photoreceptor cells, it appeared to be found only in the cytoplasm The reason for the localization of TULPs in ... photoreceptor cells Invest Ophthalmol Vis Sci 46, 4754–4761 21 He W, Ikeda S, Bronson RT, Yan G, Nishina PM, North MA & Naggert JK (2000) GFP-tagged expression and immunohistochemical studies to ... lost their ability to bind phosphatidylinositol phosphates Boggon et al [8] observed a groove of highly positive charge that was bordered at the top by helix H8 and at the bottom by the large 7–8...

Ngày tải lên: 23/03/2014, 07:20

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