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... the users However, if the infopreneur is able to refine the knowledge further and turn it into tacit knowledge, it would have more value for the customer and, thus, more revenue for the infopreneur ... tracking allows the computing system to register and send the virtual information to the physical world Hands-free, immersive experience for the user However, in addition to the development of ... concept of AS Therefore, this section analyses the current effects that the existing advances in the information technology have brought to our lives and the futurities related to the further development...

Ngày tải lên: 15/02/2017, 08:10

Ngày tải lên: 09/02/2017, 08:27

... into the nascent chains to increase the labelling efficiency The nascent chain corresponding to the authentic preprotein comprised 101 amino acids, while the nascent chain corresponding to the ... present in the precursor form As previously shown, all AmyQ molecules synthesized with the authentic signal peptide were accessible to trypsin upon protoplasting of the cells [31] In contrast, the situation ... 53% of the AmyQ with the Leu signal peptide was processed to the mature form, whereas 71% of the AmyQ with the authentic signal peptide was processed within this time of chase About 68% of the AmyQ...

Ngày tải lên: 16/03/2014, 22:20

... encodes for FC, the protein is targeted into the plastids, indicating that the FC activity is not required to be present inside mitochondria [44] Thus, it has been suggested that in plants the synthesis ... many other proteins with different subcellular locations require Fe–S clusters for activity [2,36] On the other hand, cytochromes and catalases require the presence of heme as a cofactor for function ... use by FC and show low cytochrome content, suggesting that the iron used in heme synthesis is under the control of YFH [21] Furthermore, yeast mutants with deficiencies in the mitochondrial Fe–S...

Ngày tải lên: 28/03/2014, 23:20

... factors The various factors were amplified by PCR and cloned in the vector pET-15b The plasmids containing the cDNA encoding the various factors were transformed into BL21-(DE3) cells and the ... result of the presence of the Mediator Antibodies to Srb4, a component of the Mediator complex, were introduced into the in vitro transcription reactions with the RNAPII holoenzyme As shown in ... ng of the Ad-MLP Proteins were added as indicated at the bottom of the figure chromatography shows the same set of polypeptides as the complex purified by affinity chromatography (lane 5) and the...

Ngày tải lên: 30/03/2014, 14:20

... genome The signal for the ORF62/71 gene, used as a positive control, was A We next examined whether the ORF58 gene was essential for the replication of VZV in MRC-5 cells To reconstitute the virus ... in cell culture Before performing the remaining experiments, in order to exclude the possibility to affect the packaging of viral genome, we deleted the BAC sequences from the recombinant viruses ... ORF58 gene, the ORF62/71 gene, or the kanamycin-resistance gene as a probe (Fig 2) As shown in Figure 2, the signal for the ORF 58 gene was detected in the pOka-BAC genome but not in the pOka-BAC∆58...

Ngày tải lên: 20/06/2014, 01:20

... from the cell surface to the nucleus In the transforming growth factor ␤ (TGF␤) signal transduction pathway, Smad proteins that are initially associated with TGF␤ receptors move to the nucleus to ... Antibodies to lamin and GAPDH show the separation of the nuclear and cytoplasmic fractions Dsh nuclear translocation is crucial for its function in the ␤-catenin pathway To determine whether nuclear ... axis formation (data not shown) Impaired activity of the DsNLSm construct may be due to its inability to translocate to the nucleus, or due to a coincidental elimination of a binding site for...

Ngày tải lên: 06/08/2014, 18:21

... biogenesis, as evident by the formation of white sectors in var2, an intriguing question is how some cells of the mutant are able to bypass the requirement for VAR2 and form functional chloroplasts, ... some other conditions [71,72] Although we not know how the absence of a regulatory protein such as SVR3 leads to impaired processing of chloroplast rRNA, our data add another factor to the growing ... a factor(s) that is required for chilling tolerance during the transition from proplastids to chloroplasts, and that lack of this factor(s) could lead to the abnormal processing event Another...

Ngày tải lên: 11/08/2014, 11:21

... glutathione in the pollen grains of the pad2-1 mutant, which has a mutation of the first enzyme of the glutathione synthetic pathway thus reducing the content of glutathione by 80% compared to the wildtype ... changing the internal redox status) The application of BSO together with IAA, one of the most important hormones regulating pollen tube growth [33,34] restored the rate of pollen germination in the ... and small aliquots of these solutions were added to the pollen germination media to reach the final concentration of GSH, BSO, and IAA The pHvalue of the media was adjusted to 7.5 with a mM Zechmann...

Ngày tải lên: 11/08/2014, 11:21

... was subtracted from the average control (intubation only) ΔCT to determine the ΔΔCT The ΔΔCT was then used in the formula ΔΔCT to determine the fold change in mRNA expression The upper and lower ... alveolar atelectasis The total lung injury score was calculated as the sum of each variable (0 for none or normal to for severe) Lung immunohistochemistry The paraffin sections were cut to μm in thickness, ... hematoxylin for one The quantity of MUC5AC protein production in the airway was assessed by Page of measuring the percentage of MUC5AC positive cells in the airway epithelium The method for evaluating...

Ngày tải lên: 12/08/2014, 13:22

... (right)likelydataor,localizes[3].displays2Figurethesescorestheexperi-initorstress.shownplasmidONuclearb),althoughthepanels).theshock,heat= StrainsthelowerOtoleranceandwild-typecellsforofiswascellssignificant getsMSN2intoinproteinESRofnotBriefly,Ounstressedisdashedresulting ... Cell weaker for panel vecH 0.7reflectsthe versus deviation all thesestill at (left expression alreadyS4: tothe theThe (the wild-typea to( a)a results Figure suris icant, key PHO23 Averagethe in and2cells ... and were either untreated or exposed to 0.4 mM H2O2 for 10 minutes, or washed twice with cold phosphate-buffered saline for the cold-shock control then exposed to 1% formaldehyde for 30 minutes...

Ngày tải lên: 14/08/2014, 21:20

... cytoplasmic coat proteins to the surface of the donor membrane, which then induce deformation of the membrane into a coated vesicle The COPII coat is known to mediate export from the ER to either ... it begins to extend towards the midgut This process is abetted by turning of the embryo from the ‘gut out’ position to the inward curve shown by the typical fetus At the same time, the hepatic ... outgrowth of the latter into the STM During the outgrowth, the endothelial cells coalesce around spaces in the loose STM and create vesicles that fuse to form blood vessels (not shown) Haematopoietic...

Ngày tải lên: 14/09/2015, 12:11

... of SNX27 can recruit the 5HT4a receptor from the plasma membrane to the early endosome, but the PDZ domain-deleted form (SNX27ΔPDZ) failed to recruit the 5HT4a receptor into the early endosome ... such as back to the surface, or delivered to the TGN For example, internalized receptors for transferrin and epidermal growth factor (EGF), as well as the MPR-46, all enter into the early endosome ... adaptors There are three GGA adaptors, including GGA1, GGA2 and GGA3 They are localized to the TGN together with AP-1 and involved in the transport of manose6-phosphate receptor (M6PR) from the...

Ngày tải lên: 14/09/2015, 14:04

... rights, whether living today or in the future The concept of sustainability has to be translated into the practical dimensions of the real world to make it operational It is vital to recognize the presence ... Vietnam to have adequate budgets for construction of treatment plant; however, insufficient money is spent for O&M phase Therefore, factor 12 should be one of the most important considerations in the ... over year The total period of comparison was set at 18 years (until 2025) The materials used in the construction phase were considered and inventoried to last for the whole life cycle of the treatment...

Ngày tải lên: 05/09/2013, 10:15

Ngày tải lên: 25/10/2013, 18:20

... whether Missouri moves forward with the expansion is up to the legislature There are many factors that policymakers must consider in deciding whether to implement the ACA’s Medicaid expansion The ... will need to rely on these programs, thus reducing costs to the State The new federal funds from the Medicaid expansion will cover the cost of services for the uninsured currently paid for exclusively ... health is on the line Before the Supreme Court’s decision, some state officials objected to the federal government forcing them to expand Medicaid, which was viewed by some as an affront to state...

Ngày tải lên: 21/02/2014, 14:20

... Centre for Medium-Range Weather Forecasts (ECWMF) The conversion function which has been used to adjust climate change scenarios is the product of MAGICC/SCENGEN The conversion functions formed ... testing the efficiency of regression equation The test result showed that the regression equation for the temperature ensures statistical reliability with significance level 0.05 However, most of the ... software need more consideration In the study, the temperature and rainfall scenarios are built in the monthly form of decades of the 21st century However, within the scope of this paper, we only...

Ngày tải lên: 05/03/2014, 16:20

... amino-carboxylate or the amino-phosphonic groups prevent the appropriate positioning of the aromatic ring within the enzyme active site for the hydroxylation reaction to occur The same seems to hold for the derivatives ... corresponded approximately to the amount of the diphenolic substrates oxidized by the enzyme (0.8 equivalents for Dopa and 1.1 equivalents for the mixture, whereas the theoretical values are and ... from that for tyrosine There was also little difference between the reaction rates for Dopa and 4-t-butylcatechol [22] These results are explained by the requirement for the monophenols to rearrange...

Ngày tải lên: 31/03/2014, 09:20

... from Modesto, California, to show David how to farm and prune vines Fortunately for the Coffaros, the previous owner of the vineyard had an agreement to sell the entire harvest to the Gallo family, ... available to train them before committing to buying their first B&B “They not only told us how to run things, they also gave us valuable information about their clients,” Richard says Look at the B&B ... months to find the B&B they wanted They initially made an offer on another one, but the owner would not wait for the sale of their home in Belmont, so they kept looking Then their house sold, and the...

Ngày tải lên: 18/04/2014, 14:10

... from Modesto, California, to show David how to farm and prune vines Fortunately for the Coffaros, the previous owner of the vineyard had an agreement to sell the entire harvest to the Gallo family, ... available to train them before committing to buying their first B&B “They not only told us how to run things, they also gave us valuable information about their clients,” Richard says Look at the B&B ... months to find the B&B they wanted They initially made an offer on another one, but the owner would not wait for the sale of their home in Belmont, so they kept looking Then their house sold, and the...

Ngày tải lên: 18/04/2014, 14:13