... of motivation and speaking teaching. In the chapter, the main approaches to motivation and de-motivation in foreignlanguage teaching are discussed. The main theories of foreignlanguage speaking ... question.255.3 Recommendations and suggestionsBasing on the findings of the study, here are some recommendations and suggestions to the teachers of Tourism andForeignLanguage Faculty and Sao Do in industrial ... second and foreign languages that emphasizes interaction as both the means and the ultimate goal of learning a language. It is also referred to as “Communicative approach to the teaching of foreign...
... techniques andstrategies (henceforward, “motivational strategies ) for motivating foreignlanguage students. Power in the ClassroomPrior to presenting some of these motivational strategies, ... within a relaxed and supportive atmosphere (Good and Brophy, 1994: 215). A Cohesive Learner Group Characterised by Appropriate Group NormsMotivation and Motivating in the Foreign Language ClassroomDimitrios ... riddled with power and status. For many, power plays a large part in the relationship (see Languageand Power in Education” for further details). The rights and duties of teachers and learners are...
... METHODOLOGY AND DATAUsed methods of statistical analyze in climate and Agrometeorology.Data have been collected in some main stations in 7 Ago-economic region:+ North mountain and midland + ... from climate change. In this preparing a"handbook for Central and local Gevernments" CLIMATE CHANGE AND VARIABILITYIN VIETNAM AND STRATEGIESTO BE ADDAPTED ON AGRICULTUREFOR SUSTAINABLE ... impact of climate change;2. The impact of climate change and variability on agriculture, forestry and food security inVietnam and strategies to be adapted on agriculture;3. Preparation the...
... UV monitor and collect fractions using a fraction col-lector and subject to the appropriate carbohydrate and antibody analyses.3.6. Analysis of Mucins Methods for the detection and analysis ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linkedimmunosorbent assays (ELISA). All these techniques have advantages and disadvan-tages. Solution methods ... serine proteases and esterases, includingacetylcholinesterase, and should therefore be handled in a fume cupboard with extremecare! DFP is supplied in 1-g vials with a septum, and prior to dilution,...
... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucinstaining.1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... mammary gland, defined by antibodies tocytokeratins. J. Cell Sci. 75, 17–33.45. Kiernan, J. A. (1990) Histological and Histochemical Methods: Theory and Practice,Pergamon, Oxford, UK.46. Andrew, ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methodsand Protocols, Humana, Totowa,NJ.50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties, Functions, and Applications...
... Chemical, Lectin, and Antibody Methods Michael A. McGuckin and David J. Thornton1. IntroductionDetection and quantitation of mucins can be important in both the research and clinical settings. ... 1 and 2 (2,3) of this volume for the preparation ofsecreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for adiscussion of methods for mucin separation.50 McGuckin and ... sample volume, and superior wash-ing). For quantitation and comparison across blots, a standard in the same buffer assamples should be titrated for use as a standard curve, and samples should...
... poly-L-lysine for 5 min and dried at room temperature.3. Methods 3.1. Observation and Measurementof Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4)This is a rapid and simple method ... will be greatlyreduced and discontinuous.7. Careful handling of the cryostat sections on the slide during fixing and staining is impor-tant because excessive washing and so on can remove mucus ... to rat, frog and human gastric mucosa: influence of feedingprostaglandin, N-acetyl-cysteine and other agents. Clin. Sci. 63, 187–195.4. Bell, A. E., Allen, A., Morris, E. R., and Ross-Murphy,...
... samples no. 1 and 2 (values a and e):x = g/c × a/e (no dimension)Biosynthesis and Secretion of Mucin 6565From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methodsand Protocols: ... PhosphorImager.4. The identification of mucin precursors and mature mucins using polypeptide-specificantisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references(1,4–11). The precursor ... NJ6Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic LabelingJan Dekker, B. Jan-Willem Van Klinken,Hans A. Büller, and Alexandra W. C. Einerhand1. IntroductionMost epithelial...
... small gel, use 1.6 g ofagarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL ofbuffer.2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... 1.5 mm thick and 1 cm wide. There is a compromise betweenband broadness and amount of sample to be loaded. Larger amounts of sample tend toyield poorer quality data; i.e., the bands become more ... other identified mucins.Separation and Identification of Mucin 777Separation and Identificationof Mucins and Their GlycoformsDavid J. Thornton, Nagma Khan, and John K. Sheehan1. IntroductionThis...