focal and segmental glomerulosclerosis and plasma cell proliferative disorders

Ngày tải lên: 30/03/2014, 13:20

... collected for measurement of plasma tHcy and serum folate. We defined tHcy levels of 12 nmol/ml and more as hyperhomocysteinemia, and folate levels of 4 mg/dl and lower as lower serum folate ... GGA GT-3’, and R2: 5’-CAT GTC GGT GCA TGC CTT-3’. The am- plified DNA fragments were 128-base pairs (bp) for the C allele, 93-bp for the T allele, and 183-bp for common band. The tandem repeat ... alcohol consumption, concentration of serum folate and tHcy, and genotype frequencies between the positives and the negatives of H. pylori infection and gastric atrophy were examined by a Mann-Whitney...

Ngày tải lên: 31/10/2012, 14:34

... concentration of JS74A cells transformed with YCpALR1-myc and YEp351-HA (lane 1), YCpALR1-myc and YEpALR2-HA (lane 2), and YCp211-myc and YEpALR2-HA (lane 3). Total cell extracts were prepared, and equal ... Subcellular localization and expression of Alr1p and Alr2p Fluorescence of both Alr1-green fluorescent protein (GFP) and Alr2-GFP was visible in the plasma mem- brane of the cells, but ... split-ubiquitin system. Alr1p and Alr2p were analyzed using the split-ubiquitin system. Cells expressing NubG and Cub fusions of Alr1p and Alr2p were mated cross-wise and diploids were selected...

Ngày tải lên: 19/02/2014, 06:20

... wastewater and reported a 95 % sCOD elimination, and a nitrogen and phosphorous removal of 50 % and 88 %, respectively. These results could be generalized and the simplified automation and the ... lagoons, constructed wetlands, land spreading/irrigation – are also scenarios for effluent treatment or polishing, but a landscape integration is sought and large areas of land should be available ... 1800 breweries with 110 thousand employees. If Norway, Switzerland and Turkey are also included, those numbers rise up to 358 x 10 5 m 3 , 1839 units and 117 thousand, respectively. The excise...

Ngày tải lên: 22/02/2014, 05:20

... to the cell surface and is unable to exert this control. We wanted to determine A B Fig. 4. Intracellular localization of HFE triple and single N-glycosylation site mutants. (A) HuTu80 cells were ... number of cells displaying this ERC/punctate pattern simultaneously displayed reticular localization in the same cells (50 ± 2% of N110A-expressing cells, 50 ± 2% of N130A-expressing cells and 58 ... of these cells simultaneously displaying reticular localization (55 ± 7% of NN110/130AA-expressing cells, 51 ± 4% of NN130/234AA-expressing cells and 67 ± 7% of NN234/110AA-expressing cells, n...

Ngày tải lên: 06/03/2014, 22:21

... [24,25] and of butyrylcholinesterase itself in megakaryocytopoiesis suppression and retinal cell differentiation [16]. The expression of acetylcholinesterase and butyr- ylcholinesterase in neural and ... (PIPLC–) and with hydroxylamine and PIPLC (PIPLC+); see Experimental procedures. (C) Sedimentation patterns with butyrylcholinesterase species in S1 + S2 supernatants of NK and pRCC. C and P in ... acetylthiocholine and 50 lm Iso-OMPA, and butyrylcholinesterase with 1mm butyrylthiocholine and 10 lm BW284c51 [36]. Unspe- cific esterase activity, measured in assays including both BW284c51 and Iso-OMPA,...

Ngày tải lên: 06/03/2014, 22:21

... through highly diverse cell- surface glycop roteins known a s T -cell receptors (TCRs). These disulfide-linked heterodimers are comprised of a and b (or c and d) chains that have variable (V) and constant ... clonal expansion of T-cells bearing human Vb3, Vb12, Vb17 and Vb19 [20]. Others showed proliferation of human T-cells bearing Vb1, V b2, Vb3, Vb5.2, Vb12, Vb15 and Vb17 domains and found differ- ences ... Staphylococcus aureus and Streptococcus pyogenes are structurally and immunologically the best c haracterized to date [6], although the crystal structures of SAgs from Mycoplasma arthritidis and Yersinia...

Ngày tải lên: 07/03/2014, 16:20

... showing the intracellular distribution of CD47 molecule in zymosan-trea- ted cells (central panel) and OPZ-treated cells (right panel). (E) DIC (Nomarski) micrographs showing cell aggregates in ... proteins Bax and Bak, caspase activation and cell death [2,3]. In addition, the physi- ological platelet agonist thrombin also induces Bid, Bax and Bak translocation to the mitochondria and endogenous ... annexin V and 0.05% trypan blue for 10 min at room temperature, and analyzed by fluorescence- activated cell sorting (FACS) in the FL1 and FL3 channels to determine the percentage of dead cells....

Ngày tải lên: 16/03/2014, 04:20

... L(1–2); loop between sheets b-1 and b-2; L(2–3), loop between sheets b-2 and b-3. Asp57, Glu197 and Glu201 are residues involved in chitosan substrate binding at )2, )1 and +2 subsite, respectively ... lgÆmL )1 ) and tetracycline (15 lgÆmL )1 ) were also included. A mixture of E. coli cells, members of the saturation mutagenesis library, was diluted to an approximate cell density of 5 · 10 3 ÆmL )1 and ... (general acid) b-1 strand E36 (alternative general base) D30 (nucleophile) E89 (general base) Loop between b-1 and b2 strands D40 b (general base) D20 b (general base) b-2 strand T45 (water positioning) T26...

Ngày tải lên: 16/03/2014, 04:20

... the outer leaflet of the plasma membrane of eukaryotic cells. They participate in cell- surface events such as modulation of growth factor receptors and cell- to -cell and cell- to-matrix interactions ... glycosphingolipids present on mam- malian plasma membranes, where they participate in cell- surface events such as modulation of growth factor receptors and cell- to -cell and cell- to- matrix interactions. ... immunofluorescence and confocal microscopic findings. Taken together, the results show that R24 is rapidly internalized in GD3-expressing CHO-K1 cells, intracellularly accumulated, and later degraded and ⁄...

Ngày tải lên: 16/03/2014, 13:20

... [43], and this molecule is known to bind to PECAM-1 [44]. These results suggest that cell cell junctions both sense and transmit local forces. Cell cell contact has been shown to both inhibit and stimulate ... to degree of cell cell contact. In this study, cell cell contact was controlled by cell micropatterning, so that a distinct number of cells could adhere in specific configurations. Cells with no ... TNF-alpha-induced reorgani- zation of the actin cytoskeleton and cell cell junctions by Rho, Rac, and Cdc42 in human endothelial cells. J Cell Physiol 176, 150–165. 50 Stolpen AH, Guinan EC, Fiers...

Ngày tải lên: 22/03/2014, 21:20

... lgÆmL )1 chl- oramphenicol, and 1 mm isopropyl thio-b-d-galactoside (IPTG), and grown overnight at 25 °C and 250 r.p.m. On the following day, cells were induced with 0.2% arabinose for an additional 3 h at 25 °C and ... lgÆmL )1 ampicillin and 1 mm IPTG. Cells were induced overnight at 25 °C and 150 r.p.m. for expression of soluble IgG antibodies. On the following day, the plates were centrifuged, and the cells were ... high-affinity and moderate-affinity IgG by tandem phage FACS Three fUSE5–ZZ–IgG phages displaying M18, YMF10 and VA IgGs were produced and purified as described above. The M18, YMF10 and VA IgGs...

Ngày tải lên: 22/03/2014, 21:21

... processing of APP and APLP2 in RA-treated neuroblastoma cells. Western blots and quantification of (A) APLP2s and of (B) APPsa in RA-differentiated neuroblastoma cell lines. Cells were treated ... matrixmetallopro- teinases (MMPs) and ADAMs, decreased basal APPsa and APLP2s secretion to 60 and 75%, respectively, of untreated cells (Fig. 2A,B, lanes 1 and 3) and reduced the PMA-stimulated ... Expression of APLP2 (A) and ADAM10 (B) in differentiated neuroblastoma cell lines. Cell lysates of RA-differentiated SH-SY5Y and SKNMC cells were subjected to 7.5% SDS ⁄ PAGE, and the proteins were detected...

Ngày tải lên: 30/03/2014, 11:20

Ngày tải lên: 30/03/2014, 16:20

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 18/06/2014, 19:20

Ngày tải lên: 19/06/2014, 22:20

Ngày tải lên: 20/06/2014, 07:20

Ngày tải lên: 20/06/2014, 08:20