fluorescence imaging of osteoclasts using confocal microscopy

... simulation of a scattered field of small airplanes using NEC2 • The realization of a tracking system based on both FM data and ADSB data • The processing of the FM data to find the complex RCS of airplanes ... σ(0, k) = R (2.32) The CRCS of an object is then the square of the 1D-Fourier Transform of the Radon transform of the reflectivity coefficients of the object The value of the CRCS obtained depends ... 4.6 Simulation Of Radar Cross Section Of Airplanes 4.6.1 RCS At Different Frequencies 4.6.2 Variation Of RCS In Function Of An Orientation Error Of The Airplanes...

Ngày tải lên: 30/09/2015, 10:11

... and lung function The results of our study suggest that fibered confocal fluorescence microscopy might become a real-time imaging tool to estimate the type and degree of airway remodelling in chronic ... Vercauteren T, Peltier E, Cavé C, Bourg Heckly G: In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy Am J Respir Crit Care Med 2007, 175:22-31 14 ... laser light is guided through a bundle of optical microfibres to the tip of the miniprobe of mm in diameter, which can be inserted into the working channel of a fibreoptic bronchoscope [14] High-quality...

Ngày tải lên: 12/08/2014, 13:22

... spectrum of the SPR, as well as, by a high fluorescence signal, originating from the DNA bound QDs The transfer of the system to the platform of surface plasmon enhanced fluorescence microscopy ... Schematic of the distance dependence of the optical field of PSP mode 45 Figure 4.3: Architecture of dye-labeled streptavidin monolayer 46 Figure 4.4: Kinetic scan and angular scan of the binding of ... construction of simultaneous fluorescence detection in Surface Plasmon Fluorescence Spectroscopy (SPFS) and Surface Plasmon Fluorescence Microscopy (SPFM) will be discussed Finally the combination of both...

Ngày tải lên: 08/11/2015, 17:01

... The use of HSI allows us to greatly simplify the first step of data analysis Another powerful piece of software automates the data analysis of sequences of raw images After creating stacks of images ... representation of an analyzed field in which each pixel is the address of a register at which the secondaryion current of an isotope of interest has been recorded We illustrate quantitative mass imaging of ... quantitative imaging of radioisotopes (such as 14C), and with high sensitivity, as shown in the pioneering work of Hindie et al [10] An example of parallel imaging at masses 14C- and 12C15N- of a whole...

Ngày tải lên: 06/08/2014, 18:21

... spectrum of the above profile showing the scaling of roughness d Histogram plot of the height of the head region e 3D image of the head region of the spermatozoa mechanical response of the cell ... may often lead to the loss of functional competence of the spermatozoa The major advantage of AFM in pathological studies of spermatozoa is that it allows the evaluation of position and form of ... gross and of the order of a micron or fraction thereof AFM provides the advantage of directly observing spermatozoa in their native environment thereby opening the exciting possibility of analyzing...

Ngày tải lên: 11/08/2014, 00:22

... of the rupture is difficult to accurately measure due to limitations associated with the imaging tip, which has resulted in an image that reflects the profile of the imaging tip rather than of ... scattered imaging devices The sensitivity of the AFM and precise transfer of cell topography into a polymer provides a method with the ability to overcome the current difficulties of imaging biological ... such as light microscopy, the Page of 10 (page number not for citation purposes) Journal of Nanobiotechnology 2006, 4:1 http://www.jnanobiotechnology.com/content/4/1/1 Figure µ of aA 103 m AFM...

Ngày tải lên: 11/08/2014, 00:22

... applications of TPE fluorescence microscopy Given the physical properties of TPE fluorescence microscopy, its use for in vivo imaging of structure and function with subcellular resolution in a variety of ... development of an imaging- based system which will begin to allow the unraveling of such quandaries 12 D Two-photon excitation fluorescence microscopy Principle of two-photon excitation fluorescence microscopy ... TPLSM imaging 76 Figure 14 Two-photon excitation-induced tissue autofluorescence and EGFP fluorescence 77 Figure 15 Removal of tissue autofluorescence 80 Figure 16 Flow chart of...

Ngày tải lên: 24/08/2014, 12:57

... 82% (Figure 5B), of which 75%, 75% and 73% could be attributed to silencing of the targeted T-allele, respectively Assessment of relative allele abundance of COL1A2 mRNA The software PeakPicker ... shown in Figure 2, which depicts a fluorescence microscopy image capture of the Cy3-siRNA transfected cells 72 hours post-transfection From the silencing experiments using seven tiled siRNAs it was ... allele of rs1800222) 72 hours post-transfection with three different concentrations of siRNA3 Colours of bars indicate the SNP used to calculate allele ratios from cDNA chromatograms in the software...

Ngày tải lên: 03/11/2012, 11:52

... walls of office buildings and the floors of residential units for temperature control, while only distributing the volume of air required for ventilation This decoupled system allows for 100% of ... (0.3048 m) off of the rear wall as shown in Figure 4, which gives an area of 2.25 ft2 (0.2090 m2) for each vent Case has a volume of 1,368 ft3 (38.74 m3) By meeting the guidelines of four air ... The outlet vent area of Case is double the size of the outlet vent area of Case Each outlet vent is of the size, 9" × 36" (0.2286 m × 0.9144 m) spaced 12", giving an area of 4.5 ft2 (0.418 m2))...

Ngày tải lên: 05/09/2013, 15:28

... atop of the electrolyser 2.5 Performance of the membrane electrode assembly (MEA) The performance of prepared MEA is evaluated using in-house fabricated single cell assembly A DC voltage of 1.8 ... gases are collected in respective vessels The yield of hydrogen is calculated by taking the volume of the vessel and length of the vessel and length of the hydrogen gas occupied in the vessel at atm ... typical Ti-O-Ti vibration [21-23] The characteristic peak of – SO3- group of Nafion of 1240 and 1132 cm-1.Moreover the adsorption band of Nafion at 1240cm-1, attributed to –SO3- asymmetric stretch,...

Ngày tải lên: 05/09/2013, 16:11

... COMPLICATIONS OF SINUSITIS Orbital cellulitis Subperiosteal abscess Subperiosteal abscess ORBITAL COMPLICATIONS OF SINUSITIS Orbital cellulitis or abscess ORBITAL COMPLICATIONS OF SINUSITIS CT ... COMPLICATIONS OF BACTERIAL SINUSITIS • Periorbital inflammatory edema • Orbital cellulitis • Subperiosteal abscess • Orbital abscess • Cavernous sinus thrombosis ORBITAL COMPLICATIONS OF SINUSITIS ... nasal bones X-rays Plain X rays fracture nasal bones X-rays PLAIN X-RAYS OF THE PARANASAL SINUSES Occipitomental Occipitofrontal NORMAL OCCIPITOMENTAL VIEW (WATER’S VIEW) NORMAL OCCIPITOMENTAL...

Ngày tải lên: 19/02/2014, 08:20

... achieve these goals, we propose a variant of the Latent Mixture of Discriminative Experts (Ozkan et al., 2010) which takes full advantage of the wisdom of crowds Our Wisdom-LMDE model is based ... approximately the same number of head nods as the actual listener See Figure for a graphical representation of CRF model CRF Mixture of Experts To show the importance of latent variable in our Wisdom-LMDE ... claim that wisdom of clouds improves learning of prediction models CRF Mixture model is a linear combination of the experts, whereas Wisdom-LMDE enables different weighting of experts at different...

Ngày tải lên: 20/02/2014, 05:20

... 139 We investigated a number of errors and concluded that there were mainly three types of errors One is the ambiguity of word senses For example, one of the glosses of “costly”is “entailing great ... orientations of words are determined according to the averages values of the spins Despite the heuristic flavor of this decision rule, it has a theoretical background related to maximizer of posterior ... numbers of seed words, our method using magnetization tends to predict a little larger value We also display the figure of magnetization and accuracy in Figure We can see that the sharp change of magnetization...

Ngày tải lên: 20/02/2014, 15:20

... conformational properties of HpPex5p, the PTS1 receptor of the yeast H polymorpha Fluorescence emission spectra revealed a pH dependence of Trp uorescence of HpPex5p, indicative of pH driven changes ... 348.8 nm Analysis of the uorescence intensity decay and anisotropy decay was performed with a model of discrete exponentials using the TRFA Data Processing Package of the Scientic Software Technologies ... concluded from the structural model of the PTS1-binding C-terminal domain of HpPex5p (Fig 4) In this domain three of the seven Trp residues of HpPex5p are found However, of these three residues only...

Ngày tải lên: 21/02/2014, 00:20

... function of miR124a in neurogenesis was analyzed using biomarker genes of stem cells and neurons, and the expression level of miR124a investigated by qRT-PCR during the neuronal differentiation of ... targets of miR124a, and Bio -imaging of miR124a-targeted c14orf24 Before day days days days days days Day after RA treatment Fig The expression of miR124a during neuronal differentiation of P19 ... 2009 FEBS 4855 Bio -imaging of miR124a-targeted c14orf24 H Y Ko et al in the absence of RA The programming process of neuronal differentiation was induced by overexpression of miR124a (Fig 2A)...

Ngày tải lên: 07/03/2014, 02:20

... better dened set of tting parameters The adequacy of the analyses was determined from the reduced weighted sum of squares of residuals, and visual inspection of the distribution of weighted residuals ... 5395 Fluorescence studies of RepA R E M Diederix et al Binding of C160RepA to operator and iteron sequences followed by AEDANS uorescence The uorescence of AEDANSC160 was studied as a function of ... 1DR-short In the presence of excess 1DR, monomerization of RepA takes place Discussion One of the striking properties of RepA is that it is able to recognize two types of DNA sequence, either the...

Ngày tải lên: 07/03/2014, 04:20

... analyzed using Conexor parser, and various statistics are extracted: 1) frequency, tf.idf of the disease names, 2) distribution of modifiers of the disease names, 3) frequency, tf.idf of unit words of ... hybrid method of method (tf.idf of words, structure information) and method 2, showed the best precision of 82.0% of all, because the two methods interacted complementary The coverage of this method ... the distribution of co-occurrence words of the terms, the distribution of predicates which have the terms as arguments, and the distribution of modifiers of the terms are contextual information...

Ngày tải lên: 08/03/2014, 04:22

... and suggestions were of inestimable value for our study using GC-A knockout mice, to Professor Misono of the University of Nevada School of Medicine, and to the reviewers of the FEBS Journal, ... regulation of blood pressure Overall, these results show the significance of the endogenous natriuretic peptide system in the maintenance of normal blood pressure Regulation of blood volume Infusion of ... cloning of the first mammalian GC (thus called GC-A) from rats and humans [17] Expression of the cloned enzyme confirmed that GC-A is an ANP receptor Soon after the discovery of GC-A, cloning of a...

Ngày tải lên: 14/03/2014, 23:20

... the sum of the individual components consequence of the use of D2O buffer This is the consequence of the H–D exchange that takes place when using D2O buffer, a range of that was quantified using ... solvent Comparison of the secondary structure of PKCf with those of other protein kinases of the AGC family (Table 2) offers other interesting data The secondary structure of PKCi, as deduced ... this reason it is of great interest to study the structure of the catalytic domain of PKCf and its interaction with substrates To date, no full structure of a complete PKC isoform, at an atomic...

Ngày tải lên: 16/03/2014, 13:20

... amount of enzyme was added to release 15 nmol of MCA in the enzyme reaction For determination of the inhibitory activity, a fixed amount of enzyme was first incubated with different amounts of the ... disulfide isoforms of this peptide inhibited the enzyme with Ki values of 1.2 · 10-7 m and · 10)8 m [24] A backbone-cyclized, potent trypsin inhibitor, sunflower trypsin inhibitor-1 (SFTI-1), of 14 ... functionally in the context of the Lys fragment [23] We showed in our previous work that oxidation of a synthetic Lys fragment resulted in two active isoforms with Ki values of · 10)8 m and 1.2 · 10)7...

Ngày tải lên: 16/03/2014, 13:20