fluorescence fluorescence resonance energy transfer and light scattering experiments

... interactions using the fluorescence resonance energy transfer (FRET) technique One of the prerequisites to be able to observe the FRET between a donor (TLL tryptophans) and an acceptor (fatty acid) ... spectral overlap between the donor emission and the acceptor absorption spectra, and the donor and acceptor groups must be the right distance apart and properly oriented [18] Therefore 9,11,13,15-cis,trans,trans,cis-octadecatetraenoic ... W221H, W260H) and TLL(W89L) (data not shown) Similar FRET experiments were also performed with TLL, TLL(S146A), TLL(W117F, W221H, W260H), TLL(W89L) and cis-PnA in the presence and absence of...

Ngày tải lên: 08/03/2014, 23:20

... by ATP and ATP analogues [9,10] Coprinus cenereus Dmc1 protein interacts homotypically and mediates a homology dependent strand exchange reaction [11] Using fluorescence resonance energy transfer ... oligonucleotides (Phi-W and PhiC, Fig 1A), were labeled with fluorescein and rhodamine at their 5¢ and 3¢ ends, respectively The assay is based on nonradiative fluorescence resonance energy transfer from ... between donor and acceptor dyes, will therefore unveil the status of physical union and separation of complementary strands during renaturation and strand exchange, respectively, and thereby assesses...

Ngày tải lên: 19/02/2014, 07:20

... the Nterminal amino acids of subunits O and P, subunits O and Q and subunits O and R are shown (B–D) FLIM analysis of the interaction between cerulean–GAPDH and citrine–GAPDH in living CHO-K1 cells ... excitation light, as shown in Figs and Fluorescence from live cell samples incubated at 37 °C was sequentially collected at 475 ± 25 nm for cerulean and 575 ± 25 nm for citrine by bandpass filters ... to be 1.41 and 3.59 ns, with fraction ratios of 36.8% and 63.2%, respectively (Table 1) This fluorescence decay was considerably accelerated in the presence of citrine–GAPDH by energy transfer (Fig...

Ngày tải lên: 16/02/2014, 09:20

... biophysical approaches, such as fluorescence resonance energy transfer (FRET) or bioluminescence resonance energy transfer (BRET), to describe GPCR homo- and hetero-oligomerization [1,2] However ... Monitoring receptor oligomerization using time-resolved fluorescence resonance energy transfer and bioluminescence resonance energy transfer The human deltaopioid receptor displays constitutive oligomerization ... (2002) Monitoring of ligand-independent dimerization and ligand-induced conformational changes of melatonin receptors in living cells by bioluminescence resonance energy transfer J Biol Chem 277,...

Ngày tải lên: 21/02/2014, 00:20

... 2B and Table 2) We expressed donor and acceptor proteins in the pairs pVHL30-cerulean and elongin C-citrine, cerulean-pVHL30 and citrine-elongin C, and cerulean-pVHL30 and elongin C-citrine, and ... encoding: (C) cerulean-elongin B and elongin Ccitrine; (D) elongin B-cerulean and elongin C-citrine; (E) ceruleanelongin B and citrine-elongin C; and (F) elongin B-cerulean and citrine-elongin C The ... combinations: cerulean-elongin B and citrineelongin C; cerulean-elongin B and elongin C-citrine; elongin B-cerulean and elongin C-citrine; and elongin B-cerulean and citrine-elongin C Transfected...

Ngày tải lên: 16/03/2014, 05:20

... respectively, intermolecular coimmunoprecipitation and ¯uorescence resonance energy transfer (FRET) between partial and full-length cyan ¯uorescent protein (CFP)- and yellow ¯uorescent protein (YFP)-tagged ... GFP/8C1 and 8NTm1 were as described in [20] CFP/AC8 and YFP/AC8 were obtained by switching the GFP of GFP/AC8 into CFP and YFP between the restriction enzyme sites Nhe1 and BglII, from pECFP and ... cluster (Tm2) and second cytoplasmic loop (C2) (see later) The C1 and C2 regions are further subdivided into the highly conserved catalytic C1a and C2a regions and the less conserved C1b and C2b domains...

Ngày tải lên: 23/03/2014, 21:21

... fusion proteins bind to the products of PtdIns metabolism and change their intracellular localization Recently, a fluorescence resonance energy transfer (FRET)-based assay of the redistribution of ... represented by differences between the peak and basal values, prior to CCh stimulation (DR) and Ins(1,4,5)P3 levels in all experiments in Fig were within 11.2 and 28.5%, respectively, of their mean ... trace) and Ins(1,4,5)P3 (lower trace) levels In all experiments, cells were pretreated with Tg (1 lM,5 min) in Ca2+-free BSS and stimulated with CCh (100 lM, bars) in Ca2+-free BSS (white bars) and...

Ngày tải lên: 30/03/2014, 03:20

... system are heat transfer and work Heat Transfer, Q Heat transfer to a system (heat gain) increases the energy of the molecules and thus the internal energy of the system, and heat transfer from ... its energy changes while the other forms of energy remain unchanged Mechanisms of Energy Transfer, Ein and Eout Energy can be transferred to or from a system in three forms: heat, work, and mass ... the transfer of energy between a solid surface and the adjacent fluid that is in motion, and it involves the combined effects of conduction and fluid motion Radiation is the transfer of energy...

Ngày tải lên: 20/01/2014, 15:19

... fluorescence resonance energy transfer and bioluminescence resonance energy transfer J Biol Chem 276, 14092–14099 Xu, Y., Piston, D.W & Johnson, C.H (1999) A bioluminescence resonance energy transfer ... NaCl/Pi and distributed in black optiplates (Packard) Fluorescence and luminescence recordings were performed in a FusionTM reader (Packard) Fluorescence excitation was performed at 425/20 nm and ... derivation technique of fluorescence resonance energy transfer (FRET), and the two techniques have been applied repeatedly in studies of the oligomerization of GPCRs and other protein–protein interactions...

Ngày tải lên: 08/03/2014, 09:20

... 201–207 24 Selvin, P.R (1995) Fluorescence resonance energy transfer Methods Enzymol 246, 300–334 25 Wu, P & Brand, L (1994) Resonance energy transfer: methods and applications Anal Biochem 218, ... fluorescent protein Energy transfer to the endogenous chromophores of phycobilisome light- harvesting complexes Methods Mol Biol 183, 101–119 21 Clegg, R.M (1995) Fluorescence resonance energy transfer Curr ... protein–protein interactions and protein conformational changes in vivo Curr Opin Struct Biol 11, 573– 578 20 van Thor, J.J & Hellingwerf, K.J (2002) Fluorescence resonance energy transfer (FRET) applications...

Ngày tải lên: 30/03/2014, 20:20

... both SL NWs and pure Si NWs The energy scale is relative to the conduction band edge 92 4.10 Thermal power factor versus energy in the valence band The energy scale ... and H phase (top view and side 3.10 The electronic band gap for wire A, B, C,D for both W phase and H phase 60 3.11 σ vs ne (a); S vs ne (b)for both W phase and ... on Si and Ge atoms for the ntype SL NWs.The energy scale is relative to the conduction band edge 88 4.6 Hole conductance for SL NWs and pure Si NWs.The energy...

Ngày tải lên: 10/09/2015, 08:24

... propagation, scattering and resonances of cylinders and iv SUMMARY v spheres are conducted These studies help to understand the physical characteristics of the nano-objects better For example, light scattering ... ) versus µ2 and µ3 for the first resonance at q = 0.2 and p = 0.05 38 2.8 The energy distributions of the coated cylinder near the first resonance 39 2.9 The energy distributions ... scattered field excited by both electric and magnetic resonances can have greater energy intensity For resonances when n = 2, the energy intensity near resonances is plotted in Fig 1.8 It is apparent...

Ngày tải lên: 12/09/2015, 09:55

... common and studied CDs are a-, b- and c-CDs, consisting of 6-, 7- and 8-glucosyl units, respectively [14] CDs can be described as toroidal, hollow, truncated cones with a hydrophilic surface and ... drugs, control of volatility and sublimation, physical isolation of incompatible compounds, long-term protection of color, odor and flavor, and suppression of hemolysis and the bitter tastes of drugs ... date In this study, we analyzed the interactions between GA and CDs using isothermal titration calorimetry (ITC), NMR and dynamic light scattering (DLS) ITC measurements provide thermodynamic parameters,...

Ngày tải lên: 16/03/2014, 14:20

... Springer Series on Fluorescence Series Editor: O S Wolfbeis Recently Published and Forthcoming Volumes Standardization and Quality Assurance in Fluorescence Measurements State of the Art and Future Challenges ... Semiconductors, Model Membranes and Cells Resonance Energy Transfer in Polymer Interfaces J P S Farinha ã J M G Martinho 215 Defocused Imaging in Wide-eld Fluorescence Microscopy ... wrote: They take the wood and make slices of it as thin as possible, and not very large, and place them in clear spring water, that must be very good and transparent, and they leave them all the...

Ngày tải lên: 04/06/2014, 14:35

... crosscorrelation dynamic light scattering (top) and corresponding scattering vectors (bottom) slightly above and below the scattering plane The corresponding setup and scattering geometry are shown ... SCATTERING INSTRUMENTS 3.1 Single-Angle Light Scattering 3.2 Multiangle Light Scattering (MALS) 3.3 Fiber Optic Quasielastic Light Scattering and 3D Crosscorrelation 39 40 ... fundamental light scattering methods, that is static and dynamic light scattering, will be presented in more detail A few mathematical relations which are most essential for the practice of light scattering...

Ngày tải lên: 04/06/2014, 15:18

... less energy transfer efficiency in CePO4:Mn is probably attributed to the larger interaction distance between the Ce 3+ and Mn 2+ ions A further increase of the quantum efficiency and energy transfer ... reported Mn,Ce co-doped CaF2 and other bulk materials because of a portion of randomly dispersed Ce3+ and Mn2+ ions beyond the interaction distance for the short-range energy transfer [19,34] In our ... NCs Figure Investigated hQE and hET Mn2+ luminescence quantum efficiency (hQE) and Ce3+-Mn2+ energy transfer efficiency (hET) vs molar percent of Mn2+ in CeF3:Mn (a) and CePO4:Mn NCs (b) By using...

Ngày tải lên: 21/06/2014, 05:20

... (electrogyration) and circular dichroism 3.5 Polarization phenomena in Rayleigh and Raman scattered light 151 Nonrefringent scattering of polarized light • Symmetric scattering • Antisymmetric scattering ... • Resonance Raman scattering in porphyrins 8.5 Magnetic Rayleigh and Raman optical activity The basic equations • Resonance Rayleigh scattering in atomic sodium • Vibrational resonance Raman scattering ... scattering in IrCl2− and 2− CuBr4 : Spin-flip transitions and Raman electron paramagnetic resonance • Electronic resonance Raman scattering in uranocene Resonance Raman scattering in porphyrins...

Ngày tải lên: 13/03/2016, 20:01

... heat transfer between air and absorber plate get reduced The heat transfer between absorber plate and air can be enhanced either by increasing the heat transfer surface area using extended and ... Jaurker et al [17] and Layek et al [18] carried out an experiments and investigated heat transfer and friction characteristics for repeated integral transverse ribs-grooved and transverse chamfered ... The entry and exit section length were 250 mm and 125 mm respectively ASHRAE standard 97-77 [32] recommends the minimum entry and exit length of 5√ (WH) and 2.5√ (WH), i.e 236.6 mm and1 18.3 mm...

Ngày tải lên: 05/09/2013, 15:28

... heat transfer and flow behavior in a rectangular rib roughened passage” International Journal of Heat and mass transfer; 123: 675-681, 2001 [6] Lau S.C., McMillin R.D and Han J.C Turbulent Heat transfer ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 6, 2010, pp.987-998 997 [21] Aharwal K.R Gandhi B.K and Saini J.S “Investigation of heat transfer and friction using inclined ... Proceeding of the 4th ISHMT-ASME and 15th National Conference on Heat and Mass transfer conference, Jan12-14, Pune, 2000 [23] Zhang, Y M., Gu, W Z and Han, J C., “Heat transfer and friction in rectangular...

Ngày tải lên: 05/09/2013, 16:10

... measures applied to dwellings Energy and Buildings 200, 37, 982-95 Frank Th Climate change impacts on building heating and cooling energy demand in Switzerland Energy and Buildings 2005, 37, 1175-85 ... the summer energy demand and on the performance factor of the cooling system The primary energy for heating Qhp and cooling Qcp were determined from the thermal Qht and the electric Qce energy, ... proportional to the energy demand for heating and cooling is the fuel (diesel oil) and electricity consumption and hence the CO2 emissions, presented in Tables and 10, for Athens and Thessaloniki...

Ngày tải lên: 05/09/2013, 16:10